WO2000075289A8 - Compositions and methods for targeted gene insertion - Google Patents

Compositions and methods for targeted gene insertion

Info

Publication number
WO2000075289A8
WO2000075289A8 PCT/US2000/015783 US0015783W WO0075289A8 WO 2000075289 A8 WO2000075289 A8 WO 2000075289A8 US 0015783 W US0015783 W US 0015783W WO 0075289 A8 WO0075289 A8 WO 0075289A8
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
targeted gene
gene insertion
negative selection
Prior art date
Application number
PCT/US2000/015783
Other languages
French (fr)
Other versions
WO2000075289A1 (en
Inventor
Eric Lam
Original Assignee
Univ Rutgers
Eric Lam
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rutgers, Eric Lam filed Critical Univ Rutgers
Priority to AU54744/00A priority Critical patent/AU5474400A/en
Publication of WO2000075289A1 publication Critical patent/WO2000075289A1/en
Publication of WO2000075289A8 publication Critical patent/WO2000075289A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

DNA constructs and methods for targeted integration of heterologous genes into a genome are disclosed. A transposon/transposase system is used to generate the substrates for homologous recombination in transgenic organisms. Targeted integration is selected in progeny using a combination of positive and negative selection markers. By eliminating the negative selection, the constructs and methods are adaptable for performing activation tagging, which relies on random integration events.
PCT/US2000/015783 1999-06-08 2000-06-08 Compositions and methods for targeted gene insertion WO2000075289A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU54744/00A AU5474400A (en) 1999-06-08 2000-06-08 Compositions and methods for targeted gene insertion

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60/138,068 1999-06-08
US13806899P 1999-06-11 1999-06-11

Publications (2)

Publication Number Publication Date
WO2000075289A1 WO2000075289A1 (en) 2000-12-14
WO2000075289A8 true WO2000075289A8 (en) 2004-02-19

Family

ID=33297804

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/015783 WO2000075289A1 (en) 1999-06-08 2000-06-08 Compositions and methods for targeted gene insertion

Country Status (2)

Country Link
AU (1) AU5474400A (en)
WO (1) WO2000075289A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6891082B2 (en) 1997-08-01 2005-05-10 The Johns Hopkins University School Of Medicine Transgenic non-human animals expressing a truncated activintype II receptor
CA2460617A1 (en) * 2001-08-28 2003-03-13 Japan Tobacco Inc. Method of modifying genome in higher plant
US7164056B2 (en) 2002-05-03 2007-01-16 Pioneer Hi-Bred International, Inc. Gene targeting using replicating DNA molecules
CA2767724A1 (en) 2009-07-23 2011-01-27 Chromatin, Inc. Sorghum centromere sequences and minichromosomes

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5225341A (en) * 1990-07-19 1993-07-06 The Regents Of The University Of California Biologically safe plant transformation system using a ds transposon

Also Published As

Publication number Publication date
AU5474400A (en) 2000-12-28
WO2000075289A1 (en) 2000-12-14

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