WO2000071159A1 - Use of antibodies identifying the interleukin-2 receptor for preventing and/or treating hiv infections - Google Patents
Use of antibodies identifying the interleukin-2 receptor for preventing and/or treating hiv infections Download PDFInfo
- Publication number
- WO2000071159A1 WO2000071159A1 PCT/FR2000/001399 FR0001399W WO0071159A1 WO 2000071159 A1 WO2000071159 A1 WO 2000071159A1 FR 0001399 W FR0001399 W FR 0001399W WO 0071159 A1 WO0071159 A1 WO 0071159A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- antibodies
- receptor
- interleukin
- preventing
- treating hiv
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
Definitions
- the invention relates to the use of antibodies recognizing the interleukin-2 receptor in the preparation of a medicament intended for the prevention and / or treatment of infections by an AIDS virus, in a mammal.
- AIDS viruses include human immunodeficiency viruses (HIN), simian immunodeficiency viruses (SIN) and feline immunodeficiency viruses (FIN).
- HIN human immunodeficiency viruses
- SIN simian immunodeficiency viruses
- FIN feline immunodeficiency viruses
- the invention originates from the discovery of an analogy of three-dimensional structure between zones of interleukin-2 (IL-2) participating in the binding of IL-2 on the ⁇ , ⁇ , and ⁇ of the IL-2 receptor, and envelope proteins of AIDS viruses, in particular protein gp41 for HIN and SIN, gp36 for FIN, when these proteins are in the form of trimers. These trimers have already been described in the literature.
- Another approach to vaccination against infections caused by AIDS viruses is a vaccine agent based on an immunogenic and not IL2-like envelope protein polypeptide (or a similar peptide mimicking said polypeptide).
- an immunogenic and not IL2-like envelope protein polypeptide or a similar peptide mimicking said polypeptide.
- This is the case in particular of the polypeptide 650-690, in particular 660-680 (in particular 667-672) of the protein gp41 of HIN1.
- the numbering used here is that of the SWISSPROT bank (version 34), and reference is made in particular to the sequence having the code
- E ⁇ N_HN1BR E ⁇ N_HN1BR.
- any peptide homologous to the envelope protein of FIN or SIN in the case of a vaccine against these viruses.
- the formulation of the vaccine compositions, and their mode of administration are known per se and will not be described further here.
- RIL-2 interleukin-2 receptor
- lymphocytes There are several types of cells, lymphocytes and others, which express on their surface one or more subunits of the IL-2 receptor.
- antibodies or antibody fractions containing paratopes directed against the IL-2 receptor, to block, on the receptors of the target cells of the virus, the sites of interaction with the IL-2 and / or their vicinity, and thus prevent the attachment of viral particles to these cells and / or the penetration of said viral particles into said cells, in order to prevent or limit the infection of subjects having been exposed, or dreading d have been exposed to the virus.
- infection here designates the penetration of the virus into the target cells.
- the antibodies or antibody fraction used are those which recognize at least one of the ⁇ , ⁇ and ⁇ subunits of the IL-2 receptor, including the antibodies which recognize more precisely the binding site of the IL-2 on its receiver. In both cases, these antibodies can prevent the virus from attaching to the target cells, if only by steric hindrance.
- analogs of the antibodies which have just been mentioned.
- analog is understood here to mean any peptide mimicking the paratopes of these antibodies, or alternatively any peptide capable of binding to at least one of the ⁇ , ⁇ and ⁇ subunits of the IL-2 receptor.
- Such peptides include fragments of IL-2 containing its binding site on the RIL-2 receptor, as well as any peptide capable of mimicking the binding site of IL-2 on its receptor.
- This definition of analogs excludes IL-2 itself, given the known risks associated with the undesirable side effects of IL-2, especially at large doses.
- the antibodies directed against the IL-2 receptor of the subject to be protected can be obtained according to the usual methods. Some have already been described. They may in particular be polyclonal antibodies purified by affinity chromatography, monoclonal antibodies, chimeric antibodies as described in patent FR-2,641,468, or bispecific antibodies recognizing two of the IL-2 receptor subunits.
- the invention extends to the use of any antibody fragment containing a paratope directed against the IL-2 receptor, for example Fab, Fab 'and F (ab') 2 fragments.
- an antibody is considered to have no marked immunosuppressive effect when, in conventional tests for lymphoproliferation in vitro (induced for example by the pokeweed mitogen, concanavalin A, or IL-2 ), the inhibition, caused by the antibody, of proliferation, does not exceed 25%, and in particular does not exceed 20%.
- Such non-immunosuppressive antibodies can therefore be selected by simple routine experiments.
- the amounts of antibody or antibody fractions required to block RIL-2 sites can be determined, for each species and for each antibody, by simple routine in vitro experiments. The same is true for the peptide analogues of these antibodies.
- the medicament obtained according to the invention can be in the form of injectable solutions or in the form of lyophilized preparations making it possible to reconstitute an injectable solution at the time of use.
- a medicament can be administered in vivo intravenously, or ex vivo by addition to blood samples intended to be reinjected.
- blood samples intended to be reinjected for example, between 1 and 100 mg of active principle are administered per kg of body weight per day.
- molar doses equivalent to those just indicated are used. Treatment is continued as long as the viral load of the treated individual is detectable.
- Anti-RIL2 antibodies 33B31 also called ARIL-2 (anti- ⁇ RIL2); xlone A41
- the cells are preincubated with at least one of the anti-RIL2 antibodies at a temperature of 4 ° C., one hour before being brought into contact with the virus.
- the possible intracellular penetration of the virus is revealed with a fluorescent antibody (clone KC57 FITC) directed against the core of the virus.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Communicable Diseases (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Oncology (AREA)
- Public Health (AREA)
- AIDS & HIV (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00931335A EP1178827A1 (en) | 1999-05-21 | 2000-05-22 | Use of antibodies identifying the interleukin-2 receptor for preventing and/or treating hiv infections |
APAP/P/2001/002354A AP2001002354A0 (en) | 1999-05-21 | 2000-05-22 | Use of antibodies recognizing the interleukin-2 receptor in the prevention and /or treatment of infections by the AIDS viruses. |
CA002373991A CA2373991A1 (en) | 1999-05-21 | 2000-05-22 | Use of antibodies identifying the interleukin-2 receptor for preventing and/or treating hiv infections |
AU49303/00A AU4930300A (en) | 1999-05-21 | 2000-05-22 | Use of antibodies identifying the interleukin-2 receptor for preventing and/or treating hiv infections |
EA200101228A EA200101228A1 (en) | 1999-05-21 | 2000-05-22 | USE OF ANTIBODIES THAT RECOGNIZE THE INTERLEUKIN-2 RECEPTOR FOR THE PREVENTION AND / OR TREATMENT OF INFECTIONS CAUSED BY AIDS VIRUSES |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR99/06528 | 1999-05-21 | ||
FR9906528A FR2793691B1 (en) | 1999-05-21 | 1999-05-21 | USE OF ANTIBODIES RECOGNIZING THE INTERLEUKIN-2 RECEPTOR IN THE PREVENTION AND / OR TREATMENT OF AIDS VIRUS INFECTIONS |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000071159A1 true WO2000071159A1 (en) | 2000-11-30 |
Family
ID=9545913
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FR2000/001399 WO2000071159A1 (en) | 1999-05-21 | 2000-05-22 | Use of antibodies identifying the interleukin-2 receptor for preventing and/or treating hiv infections |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1178827A1 (en) |
AP (1) | AP2001002354A0 (en) |
AU (1) | AU4930300A (en) |
CA (1) | CA2373991A1 (en) |
EA (1) | EA200101228A1 (en) |
FR (1) | FR2793691B1 (en) |
OA (1) | OA12311A (en) |
WO (1) | WO2000071159A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7438907B2 (en) | 2002-11-15 | 2008-10-21 | Genmab A/S | Human monoclonal antibodies against CD25 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0340604A2 (en) * | 1988-05-06 | 1989-11-08 | Innothérapie S.A. | Monoclonal antibody and its use |
JPH024800A (en) * | 1988-06-24 | 1990-01-09 | Ube Ind Ltd | Module for removing interleukin ii receptor and method for removing interleukin ii receptor using said module |
EP0449769A1 (en) * | 1990-03-16 | 1991-10-02 | Sandoz Ltd. | CD 25 binding molecules |
WO1993011238A1 (en) * | 1991-12-06 | 1993-06-10 | Sumitomo Pharmaceuticals Company, Limited | Humanized b-b10, an anti-il2 receptor antibody |
-
1999
- 1999-05-21 FR FR9906528A patent/FR2793691B1/en not_active Expired - Fee Related
-
2000
- 2000-05-22 EA EA200101228A patent/EA200101228A1/en unknown
- 2000-05-22 AP APAP/P/2001/002354A patent/AP2001002354A0/en unknown
- 2000-05-22 AU AU49303/00A patent/AU4930300A/en not_active Abandoned
- 2000-05-22 OA OA1200100307A patent/OA12311A/en unknown
- 2000-05-22 EP EP00931335A patent/EP1178827A1/en not_active Withdrawn
- 2000-05-22 WO PCT/FR2000/001399 patent/WO2000071159A1/en not_active Application Discontinuation
- 2000-05-22 CA CA002373991A patent/CA2373991A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0340604A2 (en) * | 1988-05-06 | 1989-11-08 | Innothérapie S.A. | Monoclonal antibody and its use |
JPH024800A (en) * | 1988-06-24 | 1990-01-09 | Ube Ind Ltd | Module for removing interleukin ii receptor and method for removing interleukin ii receptor using said module |
EP0449769A1 (en) * | 1990-03-16 | 1991-10-02 | Sandoz Ltd. | CD 25 binding molecules |
WO1993011238A1 (en) * | 1991-12-06 | 1993-06-10 | Sumitomo Pharmaceuticals Company, Limited | Humanized b-b10, an anti-il2 receptor antibody |
Non-Patent Citations (6)
Title |
---|
BELL K., ET AL.: "An immunotoxin directed against cd25 selectively kills hiv-infected pb", INTERNATIONAL CONFERENCE ON AIDS AND THE STD WORLD CONGRESS., XX, XX, vol. 02., 19 July 1992 (1992-07-19), XX, pages B160., XP002134285 * |
DATABASE WPI Week 9008, Derwent World Patents Index; AN 1990-053531, XP002134286 * |
J. BORVAK ET AL.: "Expression of CD25 defines peripheral blood mononuclear cells with productive versus latent HIV infection.", THE JOURNAL OF IMMUNOLOGY, vol. 155, no. 6, 15 September 1995 (1995-09-15), Baltimore, MD, USA, pages 3196 - 3204, XP002134283 * |
K. BELL ET AL.: "Combined use of an immunotoxin and cyclosporine to prevent both activated and quiescent peripheral blood T cells from producing type 1 human immunodeficiency virus.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE U.S.A., vol. 90, no. 4, 15 February 1993 (1993-02-15), Washington, DC, États-Unis, pages 1411 - 1415, XP002134282 * |
M. KOTANI ET AL.: "Generation of monoclonal antibodies to the rabbit interleukin-2 receptor alpha chain (CD25) and its distribution in HTLV-1-transformed rabbit T cells.", JAPANESE JOURNAL OF CANCER RESEARCH, vol. 84, no. 7, July 1993 (1993-07-01), Japon, pages 770 - 775, XP000891641 * |
O. RAMILO ET AL.: "Role of CD25+ and CD25- T cells in acute HIV infection in vitro.", THE JOURNAL OF IMMUNOLOGY, vol. 11, no. 1, 1 June 1993 (1993-06-01), Baltimore, MD, USA, pages 5202 - 5208, XP002134284 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7438907B2 (en) | 2002-11-15 | 2008-10-21 | Genmab A/S | Human monoclonal antibodies against CD25 |
US8182812B2 (en) | 2002-11-15 | 2012-05-22 | Genmab A/S | Human monoclonal antibodies against CD25 |
US8961968B2 (en) | 2002-11-15 | 2015-02-24 | Genmab A/S | Human monoclonal antibodies against CD25 |
US9598493B2 (en) | 2002-11-15 | 2017-03-21 | Genmab A/S | Human monoclonal antibodies against CD25 |
US10703818B2 (en) | 2002-11-15 | 2020-07-07 | Genmab A/S | Human monoclonal antibodies against CD25 |
Also Published As
Publication number | Publication date |
---|---|
EP1178827A1 (en) | 2002-02-13 |
FR2793691B1 (en) | 2003-10-03 |
FR2793691A1 (en) | 2000-11-24 |
EA200101228A1 (en) | 2002-08-29 |
OA12311A (en) | 2006-05-12 |
CA2373991A1 (en) | 2000-11-30 |
AU4930300A (en) | 2000-12-12 |
AP2001002354A0 (en) | 2001-12-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Sopori et al. | Effect of nicotine on the immune system: possible regulation of immune responses by central and peripheral mechanisms | |
FR2773156A1 (en) | NOVEL ANTI-RETROVIRAL IMMUNOGENS (TOXOIDS), NOVEL PREPARATION METHODS AND APPLICATION TO AIDS PREVENTION AND TREATMENT | |
FR2766826A1 (en) | VECTORS DERIVED FROM ANTIBODIES FOR TRANSFERRING SUBSTANCES IN CELLS | |
US7147849B2 (en) | Pharmaceutical formulation | |
EP1169057B1 (en) | Anti-hiv 1 vaccine comprising the entire or part of the tat hiv-1 protein | |
FR2622587A1 (en) | LYSYL-ARGINYL-ASPARTYL-SERINE PEPTIDE AND ITS APPLICATIONS AS A MEDICAMENT, IN PARTICULAR ANTITHROMBOTICS | |
JP3215110B2 (en) | Composition for treating chronic fatigue syndrome | |
EP1368372B1 (en) | Peptides having affinity for the gp120 viral protein and use thereof | |
CA1273304A (en) | Compositions and process for protecting lymphocytes t againtst the etiologic agent of lymphadenopathies and aquired immunodeficiency syndrome | |
WO2000071159A1 (en) | Use of antibodies identifying the interleukin-2 receptor for preventing and/or treating hiv infections | |
FR2945952A1 (en) | ANTI-CK8 ANTIBODIES FOR USE AS A MEDICINAL PRODUCT. | |
JP2003507011A (en) | IL-16 antagonist | |
US5567805A (en) | The cellular receptor for the CS3 peptide of human immunodeficiency virus | |
FR2828687A1 (en) | NOVEL PEPTIDES AND THEIR USE AS MEDICAMENTS AGAINST IVF INFECTION IN CATS | |
JP5836968B2 (en) | Method for treating IgE-mediated diseases | |
EP1041997B1 (en) | Use of citrulline peptides derived from filaggrin for treating autoimmune diseases | |
EP0095426A1 (en) | Synthetic polypeptides containing at least one sequence of the choleric B1 sub-unit toxin, and medicines containing them | |
FR2769226A1 (en) | Use of neuronal potassium channel blockers, especially kaliotoxin peptides | |
EP1015018A1 (en) | Veterinary medicine containing native or modified equine chorionic gonadotropin | |
LU88102A1 (en) | POLYADENYLIC ACID COMPLEXES WITH POLYURIDYLIC ACID | |
FR2731355A1 (en) | NOVEL IMMUNOGENS, NEW ANTIBODIES, PREPARATION METHOD AND PHARMACEUTICAL COMPOSITIONS COMPRISING THE SAME | |
FR2668488A1 (en) | New peptide(s) common to mycoplasm adhesion molecule and HIV proteins | |
Kabeya et al. | Bovine leukaemia virus envelope peptides cause immunomodulation in BALB/c mice | |
EP0375767B1 (en) | Peptides with protective properties with regard to a pathogenic virus of the hiv type in sensitive cells | |
CA3163277A1 (en) | Immunogenic conjugate intended to induce an immune response directed against interleukin-6 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2373991 Country of ref document: CA Ref document number: 2373991 Country of ref document: CA Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2000931335 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 200101228 Country of ref document: EA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 09979271 Country of ref document: US |
|
WWP | Wipo information: published in national office |
Ref document number: 2000931335 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 2000931335 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: JP |