WO2000058351A3 - ZWITTERMICIN A BIOSYNTHETIC GENE FROM $i(BACILLUS CEREUS) - Google Patents

ZWITTERMICIN A BIOSYNTHETIC GENE FROM $i(BACILLUS CEREUS) Download PDF

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Publication number
WO2000058351A3
WO2000058351A3 PCT/US2000/007570 US0007570W WO0058351A3 WO 2000058351 A3 WO2000058351 A3 WO 2000058351A3 US 0007570 W US0007570 W US 0007570W WO 0058351 A3 WO0058351 A3 WO 0058351A3
Authority
WO
WIPO (PCT)
Prior art keywords
zwittermicin
bacillus cereus
biosynthetic gene
disclosed
hosts
Prior art date
Application number
PCT/US2000/007570
Other languages
French (fr)
Other versions
WO2000058351A2 (en
Inventor
Jo Handelsman
Jocelyn L Milner
Elizabeth A Stohl
Elizabeth A Emmert
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Priority to EP00950199A priority Critical patent/EP1163347A2/en
Priority to AU63337/00A priority patent/AU6333700A/en
Priority to JP2000608050A priority patent/JP2003528569A/en
Publication of WO2000058351A2 publication Critical patent/WO2000058351A2/en
Publication of WO2000058351A3 publication Critical patent/WO2000058351A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Disclosed herein is an isolated DNA construct encoding enzymes necessary for the biological synthesis of zwittermicin A, a unique aminopolyol antibiotic produced by Bacillus cereus that is active against diverse bacteria and lower eucaryotes. Also disclosed herein are hosts transformed to contain and express the DNA construct, and a method to produce zwittermicin A using the construct and transformed hosts.
PCT/US2000/007570 1999-03-23 2000-03-22 ZWITTERMICIN A BIOSYNTHETIC GENE FROM $i(BACILLUS CEREUS) WO2000058351A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00950199A EP1163347A2 (en) 1999-03-23 2000-03-22 Zwittermicin a biosynthetic gene from bacillus cereus
AU63337/00A AU6333700A (en) 1999-03-23 2000-03-22 Zwittermicin a biosynthetic gene from bacillus cereus
JP2000608050A JP2003528569A (en) 1999-03-23 2000-03-22 Biosynthetic gene for zettermycin A from Bacillus cereus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12576999P 1999-03-23 1999-03-23
US60/125,769 1999-03-23

Publications (2)

Publication Number Publication Date
WO2000058351A2 WO2000058351A2 (en) 2000-10-05
WO2000058351A3 true WO2000058351A3 (en) 2001-10-04

Family

ID=22421339

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/007570 WO2000058351A2 (en) 1999-03-23 2000-03-22 ZWITTERMICIN A BIOSYNTHETIC GENE FROM $i(BACILLUS CEREUS)

Country Status (4)

Country Link
EP (1) EP1163347A2 (en)
JP (1) JP2003528569A (en)
AU (1) AU6333700A (en)
WO (1) WO2000058351A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8759031B2 (en) * 2007-04-12 2014-06-24 Wisconsin Alumni Research Foundation Type I polyketide synthase extender units
US8437622B2 (en) 2011-08-23 2013-05-07 Echostar Technologies L.L.C. Altering presentation of received content based on use of closed captioning elements as reference locations

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996008504A1 (en) * 1994-09-12 1996-03-21 Wisconsin Alumni Research Foundation Zwittermicin resistance gene and biocontrol bacteria with the gene
WO1996039483A1 (en) * 1995-06-06 1996-12-12 Wisconsin Alumni Research Foundation Novel bacillus cereus strain dga34
WO1996039532A1 (en) * 1995-06-06 1996-12-12 Wisconsin Alumni Research Foundation Novel bacillus cereus strain as4-12

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996008504A1 (en) * 1994-09-12 1996-03-21 Wisconsin Alumni Research Foundation Zwittermicin resistance gene and biocontrol bacteria with the gene
WO1996039483A1 (en) * 1995-06-06 1996-12-12 Wisconsin Alumni Research Foundation Novel bacillus cereus strain dga34
WO1996039532A1 (en) * 1995-06-06 1996-12-12 Wisconsin Alumni Research Foundation Novel bacillus cereus strain as4-12

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CUNDLIFFE E: "HOW ANTIBIOTIC-PRODUCING ORGANISMS AVOID SUICIDE", ANNUAL REVIEW OF MICROBIOLOGY, 1989, pages 207 - 234, XP000979961, ISSN: 0066-4227 *
MILNER JOCELYN L ET AL: "Zwittermicin a resistance gene from Bacillus cereus.", JOURNAL OF BACTERIOLOGY, vol. 178, no. 14, 1996, pages 4266 - 4272, XP002159148, ISSN: 0021-9193 *
STOHL E A ET AL: "Zwittermicin A biosynthetic cluster", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 237, no. 2, 17 September 1999 (1999-09-17), pages 403 - 411, XP004183533, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
WO2000058351A2 (en) 2000-10-05
JP2003528569A (en) 2003-09-30
AU6333700A (en) 2000-10-16
EP1163347A2 (en) 2001-12-19

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