Process for the preparation of asymmetrical 1,4- -dihydro-pyridine-dicarboxylic acid esters
Field of the invention
This invention relates to a new process for the preparation of asymmetrical 1 ,4-dihydro-pyridine-dicarboxylic acid esters. Technical background
It is known that 3,5-dimethyl-1 ,4-dihydro-2,6-dimethyl-4- -(2-nitro-phenyl)-pyhdine-3,5-dicarboxylate having the INN (International Non-proprietary Name) "nifedipine" can be prepared by the reaction of 1-(2-nitro-phenyl)-N,N'-bis-(2-nitro- -benzylidene)-methylene-diamine, methyl acetoacetate and methyl-3-amino-crotonate (HU 201 ,912). According to prior art a process of this type is only suitable for the preparation of symmetrical 1 ,4-dihydro-pyridine-dicarboxylic acid esters. It is also known that when preparing asymmetrical 1 ,4-dihydro- -pyridine-dicarboxylic acid esters by this reaction route - i.e. by a method in which the aldehyde component is present in the reaction mixture in "protected" form (in this case in form of the so-called "benzylidene" compound) - symmetrical esters are also formed which contaminate the product (EP 534,520).
It is also known that e.g. in the preparation of a mixed ester having the INN "nitrendipine" symmetrical esters are formed in a competitive reaction and contaminate the product to a large extent; this applies if the aldehyde is used either in free or protected form [Lyubomir D. Raev, Ivo C. Ivanov Arch
Pharm. (Weinheim) 322, 253-256 (1989)].
Pursuant to prior art asymmetrical 1 ,4-dihydro-pyridine- -dicarboxylic acid esters can be prepared by three types of reactions.
H. H. Fox et al [J. Org. Chem. 16, 1259 (1951)] describe the reaction of aldehydes, beta-oxo-carboxylic acid esters and enamino-carboxylic acid esters.
E. Knoevenagel [Ber. Dtsch. Chem. Ges. 31, 743 (1898)] discloses the reaction of benzylidene derivatives of beta-oxo-carboxylic acid esters with enamino carboxylic acid esters.
According to DOS 2,117,571 asymmetrical esters of pyridine dicarboxylic acids are prepared by reacting the corresponding benzylidene compound, beta-oxo-ester and ammonia. The latter process gives only a low yield for asymmetrical esters because due to the free ammonia content of the system undesired side-reactions can take place and by- -products contaminating the end-product are formed.
The chemical entity 3-ethyl-5-methyl-2-[(2-amino- -ethoxy)-methyl]-6-methyl-4-(2-chloro-phenyl)-1 ,4-dihydro- -pyridine-3,5-dicarboxylate (INN "amlodipine") is an effective coronary dilatory and antihypertensive agent. According to EP 89,167 this compound can be prepared by removing the phthaloyl group from 3-ethyl-5-methyl-2-[(2-phthalimido- -ethoxy)-methyl]-6-methyl-4-(2-chloro-phenyl)-1 ,4-dihydro- -pyridine-3,5-dicarboxylate. The phthaloyl group can be split off preferably with the aid of hydrazine. The above phthalimido
compound can be prepared by means of the method of Hantzsch by reacting phthalimido-ethoxy-acetoacetate, 2- -chloro-benzaldehyde and methyl-3-amino-crotonate. In EP 89,167 no yield is disclosed for the above cyclisation reaction. According to J. Med. Chem. 29, 1696-1702 (1986) the yield reported for said reaction is not higher than 25 %. Summary of the invention
It is the object of the present invention to provide a process for the preparation of a broad range of mixed esters of 1 ,4-dihydro-pyridine-dicarboxylic acid esters which can be carried out with high yields and gives a pure product.
The above object is solved by the process of the present invention.
According to the present invention there is provided a process for the preparation of asymmetrical 1 ,4-dihydro- -pyridine-dicarboxylic acid esters of the general Formula
(wherein
R1 and R2 each stands for lower alkyl or an ethyl group substituted by methoxy or cyano, with the proviso that
R1 and R2 are different; R3 stands for lower alkyl optionally substituted by hydroxy
or halogen, or a benzyloxymethyl group or a group of the general Formula -CH2-0-CH2-CH2-X; X stands for halogen, azido or -NR4R5; R4 and R5 are identical or different and each stands for hydrogen or lower alkyl, or R4 and R5 together with the nitrogen atom, they are attached to, form a phthaloyl group; A is nitro or halogen and n is 1 or 2) which comprises a) reacting a compound of the general Formula
( A ) n (wherein A and n are as stated above) with a compound of the general Formula
R1OOC — CH2 — C — CH3 Ml
II o and an amino crotonic acid ester of the general Formula
R2OOC — CH =C R3 V|
NH2
(wherein R1, R2 and R3 are as stated above); or b) reacting a compound of the general Formula II with
a beta-keto-carboxylic acid ester of the general Formula
R2OOC — CH2 — C R3 i
II o and an amino-crotonic acid ester of the general Formula
R1OOC — CH = C — CH_
I v
NH2
(wherein R1, R2 and R3 are as stated above) and if desired transforming a compound of the general Formula I thus obtained into another compound of the general Formula I.
Detailed description of the invention
The present invention is based on the recognition that on reacting compounds of the general Formula II either with compounds of the general Formulae III and VI, or with compounds of the general Formulae IV and V the desired mixed esters of the general Formula I are obtained with high yields and in pure form, practically free of by-products. This recognition is so much the more surprising as in spite of the complicated reaction mechanism the process of the present invention provides the desired mixed esters in highly pure form and no symmetrical esters are formed in the reaction. It could not be aforeseen that the mixed esters of the general Formula I would be formed in pure form and free of symmetrical esters.
The definitions used throughout the patent specification and the claims are to be interpreted as follows:
The term "lower alkyl" relates to straight or branched chained saturated hydrocarbon groups having 1-4 carbon atoms (e.g. methyl, ethyl, n-propyl, isopropyl, π-butyl, secondary butyl etc.).
The term "halogen" encompasses the fluorine, chlorine, bromine and iodine atoms and stands preferably for chlorine or bromine, particularly for chlorine.
The term "amino protective group" relates to groups generally known from prior art for the protection of the amino group. Such groups may be e.g. alkylsulfonyloxy (e.g. methanesulfonyloxy), benzenesulfonyloxy, p-toluenesulfonyloxy, aralkyl (e.g. benzyl) or acyl (e.g. lower alkanoyl such as acetyl; or aroyl e.g. benzoyl) etc.
According to a preferred embodiment of the process of the present invention compounds of the general Formula I are prepared in which A stands for chlorine in position 2, or nitro in position 2 or 3; n is 1 ; R1 stands for methyl; R2 represents ethyl or 2-methyl-propyl and R3 stands for methyl, hydroxymethyl, chloromethyl, phthalimido-ethoxy-methyl, benzyloxymethyl or 2-chloro-ethoxy-methyl.
According to a particularly preferred embodiment of the process of the present invention 3-ethyl-5-methyl-2-[(2- phthaiimido-ethoxy)-methyl]-6-methyl-4-(2-chloro-phenyl)-1 ,4- -dihydro-pyridine-3,5-dicarboxylate is prepared.
The process of the present invention is suitable for the preparation of calcium antagonists widespreadly used in therapy (e.g. nitrendipin, nisoldipin, felodipin, nimodipin etc.).
According to the process of the present invention valuable pharmaceutical intermediates can also be prepared. Thus the process of the present invention is suitable for the preparation of 3-ethyl-5-methyl-2-[(2-phthalimido-ethoxy)- -methyl]-6-methyl-4-(2-chloro-phenyl)-1 ,4-dihydro-pyridine-3,5- -dicarboxylate which can be used as intermediate in the manufacture of amlodipin (EP 89,167).
According to a further aspect of the present invention there is provided 3-ethyl-5-methyl-2-(benzyloxymethyl)-6- -methyl-4-(2-chloro-phenyl)-1,4-dihydro-pyridine-3,5-dicarboxylate which is a new compound of the general Formula I, never described in prior art.
According to process a) of the process of the present invention 1 mole of a compound of the general Formula II is reacted with 3-4.5 moles of a compound of the general Formula III and 3-4.5 moles of a compound of the general Formula VI. It is preferred to use 3 moles of a compound of the general Formula III and 3 moles of a compound of the general Formula VI, related to 1 mole of a compound of the general Formula II. The reaction is carried out in an inert organic solvent. As reaction medium polar protic solvents, preferably lower alkanols (e.g. methanol, ethanol, propanol, isopropanol or isobutanol), water or amides (e.g. formamide, dimethyl formamide) or dipolar aprotic solvents (e.g. acetonitrile, dimethyl sulfoxide, nitro benzene, etc.) or mixtures thereof can be used. One may work preferably by using a lower alkanol as reaction medium.
The reaction temperature can be varied between wide ranges. The reaction can be performed at a temperature between room temperature and 120°C, preferably at 30-90°C. One may work preferably at the boiling point of the solvent used as reaction medium (preferably a lower alkanol).
The reaction time may also be varied between wide ranges, depending on the reaction temperature. The reaction takes generally 1-50 hours. According to an advantageous form of realization of the process of the present invention the reaction is carried out in alkanol as medium, at the boiling point of the reaction mixture, for 10-25 hours.
The reaction conditions used for process b) of the present invention are similar to those applied for process a) (e.g. solvent, temperature, reaction time).
The reaction mixture may be worked up by methods known per se. A reaction product poorly soluble in the cold in the solvent used can be separated by cooling and subsequent filtration or centrifuging. In this case generally a second generation of the product can be recovered by evaporating the solvent. If the desired product is in solution at the end of the reaction, it can be isolated in crystalline or oily form by evaporating the solvent. The oil thus obtained can be generally crystallized. Alternatively the desired product can be precipitated from the reaction mixture by adding water or an aqueous acid. The product thus obtained can be purified, if desired, by conventional methods (e.g. recrystallization or chromatographical separation).
The compounds of the general Formula I contain a chiral carbon atom. The present invention encompasses the preparation of all optical isomers of the compound of the general Formula 1 and optional ratio mixtures thereof, including the racemates.
A compound of the general Formula I obtained by the process of the present invention can be optionally converted into another compound of the general Formula I.
Said subsequent transformations can be carried out in a known manner.
Thus e.g. a compound of the general Formula I, wherein R3 stands for benzyloxymethyl, can be converted by hydrogenation into a compound of the general Formula I wherein R3 stands for hydroxymethyl. The reaction can be carried out by catalytic hydrogenation; as catalyst e.g. palladium or platinum can be used. Hydrogenation can be performed in a solvent (e.g. acetic acid) under a pressure of 2-6 atm and at room temperature.
According to another optional subsequent transformation the phthaloyl group is removed from a compound of the general Formula I, wherein R3 stands for phthalimido-ethoxy-methyl, to yield the corresponding compound of the general Formula I wherein R3 stands for 2- -amino-ethoxy-methyl. The phthaloyl group can be removed e.g. by treatment with hydrazine.
The starting materials of the general Formula II are generally known compounds. However, 1-(2,3-dichloro-
-phenyl)-N,N'-bis-(2,3-dichloro-benzylidene)-methylene-diamine of the general Formula II is a new compound.
According to a further aspect of the present invention there is provided 1-(2,3-dichloro-phenyl)-N,N'-bis-(2,3- dichloro-benzylidene)-methylene-diamine of the general Formula II.
The preparation of 1-(2,3-dichloro-phenyl)-N,N'-bis- (2,3-dichloro-benzylidene)-methylene-diamine is carried out by reacting 2,3-dichloro-benzaldehyde with ammonia. The desired compound is obtained within a short reaction time, with high yields and in pure form. The reaction may be carried out in an aliphatic alcohol (preferably ethanol) as medium at room temperature.
The beta-keto-esters of the general Formulae III and IV used as starting material are commercially available compounds.
The starting materials of the general Formulae V and VI are also known, except the following two compounds of the general Formula VI: ethyl-4-(phthalimido-ethoxy)-3-amino-crotonate; ethyl-4-benzyloxy-3-amino-chrotonate.
According to a further feature of the present invention there are provided the following compounds of the general Formula VI: ethyl-4-(phthalimido-ethoxy)-3-amino-crotonate; ethyl-4-benzyloxy-3-amino-chrotonate.
The above two new compounds of the general Formula
VI can be prepared by reacting ammonia with ethyl-4- -phthalimido-ethoxy-acetoacetate or ethyl-4-benzyloxy- -acetoacetate, respectively. The reaction may be carried out in an aliphatic alcohol (preferably ethanol or isopropanol) as medium under heating. The reaction provides high yields.
The advantage of the process of the present invention is that the desired mixed asymmetrical 1,4-dihydro-pyridine- -dicarboxylic acid esters of the general Formula I can be obtained with good yields, in highly pure form, free of by-products. The process is suitable for industrial scale manufacture, is readily feasible and requires no special apparatus.
Further details of the present invention are to be found in the following Examples without limiting the scope of protection to said Examples.
Examples
I
Preparation of new starting materials of the general
Formula II
Example 1
1-(2,3-dichloro-phenyl)-N,N'-bis-(2,3-dichloro-benzylidene)- -methylene-diamine
8.75 g (0.05 mole) of 2,3-dichloro-benzaldehyde are stirred in 20 ml of ethanol containing 5-7 % by weight of ammonia at room temperature for 5 hours. The precipitated product is filtered, washed with ethanol and dried. Thus 8.25 g of 1-(2,3-dichloro-phenyl)-N,N'-bis-(2,3-dichloro-benzylidene)- -methylene-diamine are obtained, yield 98 %, mp.: 160-162°C. Analysis: for the Formula C21H12CI6N2 (505.063) calc: C%=49.94; H%=2.39; N%=5.55; Cl%=42.17; found: C%=49.51 ; H%=2.43; N%=5.32; Cl%=42.01.
II Preparation of new starting materials of compounds of the general Formula VI
Example 2
Ethyl-4-phthalimido-ethoxy-3-amino-crotonate
37 g (0.116 mole) of ethyl-4-phthalimido-ethoxy- -acetoacetate are heated to boiling in 240 ml of ethanol containing 9.5 % by weight of ammonia for 19 hours. The
reaction mixture is evaporated. The residual oil is crystallized at 0-5°C from isopropanol, filtered, washed with a small amount of isopropanol and dried. Thus 28.47 g of ethyl-4- -phthalimido-ethoxy-3-amino-crotonate are obtained in the form of light beige crystals. Yield 77.2 %, mp.: 77-78°C. Analysis: for the Formula Cι6H18N205 (318.331) calc: C%=60.37; H%=5.70; N%=8.79; found: C%=59.99; H%=5.78; N%=9.08. Example 3 Ethyl-4-benzyloxy-3-amino-crotonate
2.36 g (10 millimoles) of ethyl-4-benzyloxy-acetoacetate are reacted in 12 ml of ethanol containing 5-7 % by weight of ammonia at 40°C for 9 hours. The reaction mixture is evaporated. Thus 2.26 g of ethyl-4-benzyloxy-3-amino-crotonate are obtained in the form of a faint yellow oil. Yield 95.7 %. n20 = 1.5356.
D
III
Preparation of dihydro-pyridine-dicarboxylic acid esters of the general Formula I
Example 4
3-ethyl-5-methyl-2-f(2-phthalimido-ethoxy)-methvπ-6-methyl-4-
-(2-chloro-phenyl)-1 ,4-dihvdro-pyridine-3,5-dicarboxylate
Method a)
A mixture of 11.31 g (0.028 mole) of 1-(2-chloro-
-phenyl)-N,N'-bis-(2-chloro-benzylidene)-methylene-diamine, 9.8 g (0.084 mole) of methyl acetoacetate, 27 g (0,84 mole) of
ethyl-4-phthalimidoethoxy-3-amino-crotonate and 230 ml of isopropanol is heated to boiling for 26 hours. The reaction mixture is evaporated. The residual oil is crystallized from acetic acid at 5-10°C, filtered and suspended in cold (0-5°C) methanol. Thus 17.66 g of 3-ethyl-5-methyl-2-[(2-phthalimido- -ethoxy)-methyl]-6-methyM-(2-chloro-phenyl)-1,4-dihydro-pyridine- -3,5-dicarboxylate are obtained, yield 38.8 %, mp.: 147-149°C. Analysis: for the Formula C28H27CIN207 (538.986) calc: C%=62.39; H%=5.05; Cl%=6.58; N%=5.19; found: C%=62.42; H%=5.14; Cl%=6.32; N%=5.09. Method b)
A mixture of 8.86 g (0.022 mole) of 1-(2-chloro-phenyl)- N,N'-bis-(2-chloro-benzylidene)-methylene-diamine, 7.61 g (0.066 mole) of methyl-amino-crotonate, 21 g (0.066 mole) of ethyl-4-phthalimido-ethoxy-acetoacetate and 140 ml of isopropanol is heated to boiling for 26 hours. The reaction mixture is evaporated. The residual oil is worked up as described in method a). Thus 10.22 g of 3-ethyl-5-methyl-2- -[(2-phthalimido-ethoxy)-methyl]-6-methyl-4-(2-chloro-phenyl)- -1 ,4-dihydro-pyridine-3,5-dicarboxylate are obtained, yield 28.7 %. Mp.: 145-147°C.
Analysis: for the Formula C28H27CIN207 (538.986) calc: C%=62.39; H%=5.05; Cl%=6.58; N%=5.19; found: C%=63.05; H%=4.98; Cl%=6.47; N%=5.04.
Example 5
3-ethyl-5-methyl-2,6-dimethyl-4-(3-nitro-phenyl)-1 ,4-dihvdro- pyridine-3,5-dicarboxylate (nitrendipin) Method a)
A mixture of 4.33 g (10 millimoles) of 1-(3-nitro-phenyl)- -N,N'-bis-(3-nitro-benzylidene)-methylene-diamine, 5.18 g (45 millimoles) of methyl-3-amino-crotonate, 5.86 g (45 millimoles) of ethyl acetoacetate and 30 ml of isopropanol is stirred and heated under reflux for 15 hours. The solution is cooled to 0°C. After 30 minutes the precipitated crystals are filtered. The mother-lye is evaporated in vacuo and the crystals are filtered. The united crystalline product is washed with cold isopropanol and dried. Thus 9.3 g of 3-ethyl-5-methyl-2,6-dimethyl-4-(3- -nitro-phenyl)-1 ,4-dihydro-pyridine-3,5-dicarboxylate are obtained, yield 86.0 %, mp.: 156-158°C. Analysis: for the Formula Cι8H20N2O6 (360.369) calc: C%=59.99; H%=5.59; N%=7.77; found: C%=59.76; H%=5.61 ; N%=7.83. Method b)
A mixture of 4.33 g (10 millimoles) of 1-(3-nitro-phenyl)- -N,N'-bis-(3-nitro-benzylidene)-methylene-diamine, 5.81 g (45 millimoles) of ethyl-3-amino-crotonate and 5.22 g (45 millimoles) of methyl-acetoacetate are reacted in an analogous manner to method a). Thus 9.19 g of 3-ethyl-5-methyl-2,6-dimethyl-4-(3- -nitro-phenyl)-1 ,4-dihydro-pyridine-3,5-dicarboxylate are obtained, yield 85.0 %, mp.: 155-157°C.
Example 6
3-(2-methyl-propyl)-5-methyl-2,6-dimethyl-4-f2-nitro-phenyl)- -1 ,4-dihvdro-pyridine-3,5-dicarboxylate (nisoldipin)
A mixture of 4.33 g (10 millimoles) of 1-(2-nitro-phenyl)- -N,N'-bis-(2-nitro-benzylidene)-methylene-diamine, 3.45 g (30 millimoles) of methyl-3-amino-crotonate, 4,75 g (30 millimoles) of 2-methyl-propyl-acetoacetate and 30 ml of n-butanol is stirred and heated under reflux for 20 hours. The solution is evaporated in vacuo. The oily residue is purified by flash chromatography on Kieselgel 60 and elution with a 6:4 mixture of ethyl acetate and n-hexane. A warm 1 :1 mixture of ethyl acetate and n-hexane (20 ml) is poured on the oil thus obtained, whereupon the product crystallized on cooling is filtered, washed with cold ethanol and dried. Thus 2.9 g of 3- -(2-methyl-propyl)-5-methyl-2,6-dimethyl-4-(2-nitro-phenyl)-1,4- -dihydro-pyridine-3,5-dicarboxylate are obtained, yield 25 %, mp.: 142-143°C. Example 7
3-ethyl-5-methyl-2-benzyloxymethyl-6-methyl-4-(2-chloro-phenyl)- -1 ,4-dihvdro-pyridine-3.5-dicarboxylate
A mixture of 8.64 g (20 millimoles) of 1-(2-chloro- -phenyl)-N,N'-bis-(2-chloro-benzylidene)-methylene-diamine, 6.69 g (60 millimoles) of methyl-3-amino-crotonate, 13.7 g (60 millimoles) of ethyl-4-benzyloxy-acetoacetate and 200 ml of methanol is heated to boiling for 27 hours. The reaction mixture can be worked up by two methods. a) The reaction mixture is evaporated to one-third of
the original volume in vacuo and crystallized at a temperature between 0°C and 5°C. The precipitated crystals are filtered and recrystallized from methanol. Thus 11.7 g of 3-ethyl-5- -methyl-2-benzyloxymethyl-6-methyl-4-(2-chloro-phenyl)-1,4- -dihydro-pyridine-3,5-dicarboxylate are obtained, yield 40 %, mp.: 113-115°C. b) The reaction mixture is cooled to a temperature of 10°C below the boiling point, whereupon a mixture of 110 ml of 12 N hydrochloric acid, 110 ml of water and 220 ml of methanol is added dropwise within 20 minutes. The mixture is stirred at room temperature for 3 hours and allowed to stand in a refrigerator for 48 hours. After filtration 13.5 g of 3-ethyl-5- methyl-2-benzyloxymethyl-6-methyl-4-(2-chloro-phenyl)-1 ,4- -dihydro-pyridine-3,5-dicarboxylate are obtained, yield 46 %. Mp.: 111-113°C
Analysis: for the Formula C25H26CIN05 (454.932) calc: C%=66.00; H%=5.76; N%=3.08; Cl%=7.79; found: C%=66.04; H%=5.78; N%=3.13; Cl%=7.85. Example 8
3-ethyl-5-methyl-2-hvdroxymethyl-6-methyl-4-(2-chloro-phenv0- -1 ,4-dihydro-pyridine-3,5-dicarboxylate Method a)
. A mixture of 2.0 g (5 millimoles) of 1-(2-chloro-phenyl)- -N,N'-bis-(2-chloro-benzylidene)-methylene-diamine, 1.71 g (15 millimoles) of methyl-3-amino-crotonate, 2.19 g (15 millimoles) of ethyl-4-hydroxy-acetoacetate and 50 ml of methanol is heated to boiling for 20 hours. After clarification
the solution is evaporated, the residual oil is subjected to column chromatography on Kieselgel 60 and eluted with a 4:6 mixture of n-hexane and ethyl acetate. The oily residue obtained after evaporation is crystallized from diisopropyl ether. Thus 3.4 g of 3-ethyl-5-methyl-2-hydroxymethyl-6- -methyl-4-(2-chloro-phenyl)-1 ,4-dihydro-pyridine-3,5-dicarboxylate are obtained in the form of a faint yellow solid substance. Yield 62 %, mp.: 131-133°C. Method b)
7.97 g (18 millimoles) of 2-benzyloxymethyl-6-methyl-4- -(2-chloro-phenyl)-1 ,4-dihydro-pyridine-3,5-dicarboxylate are dissolved in 120 ml of ethyl acetate, whereupon 1.54 g of a Selcat-Q type palladium-charcoal catalyst and 6 drops of acetic acid are added and the mixture is hydrogenated under a pressure of 6 atm at room temperature. The catalyst is filtered off and the filtrate is evaporated. The residual yellow oil is dissolved in hot acetic acid, cooled and poured into icecold water under stirring. The precipitated yellow powder is filtered, washed, dried, suspended in diisopropyl ether, filtered and dried again. Thus 5.8 g of 3-ethyl-5-methyl-2-hydroxymethyl-6- -methyl-4-(2-chloro-phenyl)-1,4-dihydro-pyridine-3,5-dicarboxylate are obtained, yield 90.6 %. Mp.: 134-136°C. Analysis: for the Formula C18H20CINO5 (365.814) calc: C%=59.10; H%=5.51 ; N%=3.83; Cl%=9.69; found: C%=57.36; H%=5.55; N%=3.87; Cl%=9.64.
Example 9
S-ethyl-δ-methyl^-chloromethyl-e-methyl^-^-chloro-phenyl)-
-1 ,4-dihydro-pyridine-3,5-dicarboxylate
Method a)
A mixture of 2.0 g (5 millimoles) of 1-(2-chloro-phenyl)- -N,N'-bis-(2-chloro-benzylidene)-methylene-diamine, 1.71 g (15 millimoles) of methyl-3-amino-crotonate, 2.47 g (15 millimoles) of ethyl-4-chloro-acetoacetate and 50 ml of methanol is heated to boiling for 32 hours. After clarification the solution is evaporated in vacuo. The residual oil is subjected to column chromatography on Kieselgel 60 and eluted with a 4:6 mixture of n-hexane and ethyl acetate. Thus 0.57 g of 3-ethyl-5-methyl-2-chloromethyl-6-methyl-4-(2-chloro- -phenyl)-1 ,4-dihydro-pyridine-3,5-dicarboxylate are obtained in the form of an oil. Method b)
A mixture of 0.4 g (1 millimole) of 1-(2-chloro-phenyl)- -N,N'-bis-(2-chloro-benzylidene)-methylene-diamine, 0.35 g (3 millimoles) of methyl-acetoacetate and 1.0 g (3 millimoles) of ethyl-4-chloro-ethoxy-3-amino-crotonate and 7 ml of isopropanol is heated to boiling for 24 hours. The reaction mixture is clarified and evaporated. The residue is purified as described in method a). Thus 0.56 g of 3-ethyl-5-methyl-2- -chloromethyl-6-methyl-4-(2-chloro-phenyl)-1 ,4-dihydro-pyridine- -3,5-dicarboxylate are obtained, mp.: 142-144°C.
Example 10
3-ethyl-5-methyl-2-(2-chloroethoxy-methyl)-6-methyl-4-(2- -chloro-phenyl)-1 ,4-dihydro-pyridine-3,5-dicarboxylate
A mixture of 2.4 g (6 millimoles) of 1-(2-chloro-phenyl)- N,N'-bis-(2-chloro-benzylidene)-methylene-diamine, 2.7 g (18 millimoles) of methyl-3-amino-crotonate, 3.75 g (18 millimoles) of 4-(2-chloro-ethoxy)-acetoacetate and 45 ml of isopropanol is heated to boiling for 15 hours. The reaction mixture is cooled, the precipitated crystalline product is filtered, washed with cold isopropanol and diisopropyl ether and dried. The crude product is recrystallized from methanol. Thus 2.47 g of 3-ethyl-5-methyl-2-(2-chloro-ethoxy-methyl)-6-methyl-4-(2-chloro- -phenyl)-1 ,4-dihydro-pyridine-3,5-dicarboxylate are obtained in the form of butter-coloured crystals. Yield 32 %, mp.: 152- 153°C.
Analysis: for the Formula C20H23CI2NO5 (428.320) calc: C%=56.08; H%=5.41 ; Cl%=16.56; N%=3.27; found: C%=56.10; H%=5.42; Cl%=16.18; N%=3.37.