WO2000042221A3 - Method for the affinity isolation of newly synthesized rna - Google Patents

Method for the affinity isolation of newly synthesized rna Download PDF

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Publication number
WO2000042221A3
WO2000042221A3 PCT/US2000/000691 US0000691W WO0042221A3 WO 2000042221 A3 WO2000042221 A3 WO 2000042221A3 US 0000691 W US0000691 W US 0000691W WO 0042221 A3 WO0042221 A3 WO 0042221A3
Authority
WO
WIPO (PCT)
Prior art keywords
newly synthesized
synthesized rna
affinity isolation
mrna
methods
Prior art date
Application number
PCT/US2000/000691
Other languages
French (fr)
Other versions
WO2000042221A2 (en
WO2000042221A9 (en
Inventor
Jose R Pardinas
Kyle W H Chan
Original Assignee
Signal Pharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Signal Pharm Inc filed Critical Signal Pharm Inc
Priority to CA002359814A priority Critical patent/CA2359814A1/en
Priority to EP00911576A priority patent/EP1144687A2/en
Priority to AU33455/00A priority patent/AU767583B2/en
Priority to JP2000593778A priority patent/JP2002534136A/en
Publication of WO2000042221A2 publication Critical patent/WO2000042221A2/en
Publication of WO2000042221A3 publication Critical patent/WO2000042221A3/en
Publication of WO2000042221A9 publication Critical patent/WO2000042221A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Methods are provided for the isolation of newly synthesized mRNA. Such methods involve the incorporation of biotinylated rNTP analogues into cellular mRNA, and separating biotinylated mRNA from unlabeled RNA. The methods provided herein may be used, for example, for gene discovery, drug screens and studies of the regulation of gene expression.
PCT/US2000/000691 1999-01-11 2000-01-11 Method for the affinity isolation of newly synthesized rna WO2000042221A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002359814A CA2359814A1 (en) 1999-01-11 2000-01-11 Method for the affinity isolation of newly synthesized rna
EP00911576A EP1144687A2 (en) 1999-01-11 2000-01-11 Method for the affinity isolation of newly synthesized rna
AU33455/00A AU767583B2 (en) 1999-01-11 2000-01-11 Method for the affinity isolation of newly synthesized RNA
JP2000593778A JP2002534136A (en) 1999-01-11 2000-01-11 Method for affinity isolation of newly synthesized RNA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/228,455 1999-01-11
US09/228,455 US6413720B1 (en) 1999-01-11 1999-01-11 Method for the affinity isolation of newly synthesized RNA

Publications (3)

Publication Number Publication Date
WO2000042221A2 WO2000042221A2 (en) 2000-07-20
WO2000042221A3 true WO2000042221A3 (en) 2000-09-28
WO2000042221A9 WO2000042221A9 (en) 2001-11-08

Family

ID=22857241

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/000691 WO2000042221A2 (en) 1999-01-11 2000-01-11 Method for the affinity isolation of newly synthesized rna

Country Status (6)

Country Link
US (2) US6413720B1 (en)
EP (1) EP1144687A2 (en)
JP (1) JP2002534136A (en)
AU (1) AU767583B2 (en)
CA (1) CA2359814A1 (en)
WO (1) WO2000042221A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6413720B1 (en) * 1999-01-11 2002-07-02 Signal Pharmaceuticals, Inc. Method for the affinity isolation of newly synthesized RNA
US20040058330A1 (en) * 2002-09-20 2004-03-25 Prokaria, Ltd. Methods of use for thermostable RNA ligases
DE102008028080A1 (en) * 2008-06-13 2009-12-24 Thomas Reinhardt Method for obtaining fresh water, by spraying saline water by a droplet producer into droplets with size pre-determined for large part of a pre-defined size area, where the droplets are sprayed in an extensively surrounded moistening area
EP2316030B1 (en) 2008-07-25 2019-08-21 Wagner, Richard W. Protein screeing methods
US20100062946A1 (en) * 2008-09-08 2010-03-11 Lis John T Genome-wide method for mapping of engaged rna polymerases quantitatively and at high resolution

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0480408A1 (en) * 1990-10-11 1992-04-15 Asahi Kasei Kogyo Kabushiki Kaisha Method for the measurement of reverse transcriptase by immobilized primer
WO1997007244A1 (en) * 1995-08-11 1997-02-27 The United States Of America, Represented By The ISOLATION OF AMPLIFIED GENES VIA cDNA SUBTRACTIVE HYBRIDIZATION
US5888727A (en) * 1996-05-23 1999-03-30 Wisconsin Alumni Research Foundation Method of inhibition of nucleo-cytoplasmic transport by M protein of vesicular stomatitis virus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3613401A1 (en) * 1986-04-21 1987-12-17 Boehringer Mannheim Gmbh METHOD FOR PRODUCING PLASMINOGEN ACTIVATORS IN PROKARYONTS
WO1997037045A1 (en) * 1996-04-03 1997-10-09 Johnson & Johnson Consumer Products, Inc. Improved technique for differential display
US6413720B1 (en) * 1999-01-11 2002-07-02 Signal Pharmaceuticals, Inc. Method for the affinity isolation of newly synthesized RNA

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0480408A1 (en) * 1990-10-11 1992-04-15 Asahi Kasei Kogyo Kabushiki Kaisha Method for the measurement of reverse transcriptase by immobilized primer
WO1997007244A1 (en) * 1995-08-11 1997-02-27 The United States Of America, Represented By The ISOLATION OF AMPLIFIED GENES VIA cDNA SUBTRACTIVE HYBRIDIZATION
US5888727A (en) * 1996-05-23 1999-03-30 Wisconsin Alumni Research Foundation Method of inhibition of nucleo-cytoplasmic transport by M protein of vesicular stomatitis virus

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL BIOCHEMISTRY, vol. 123, no. 1, 1982, pages 94 - 100, ISSN: 0003-2697 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1982, MILLER D W ET AL: "5' MERCURI UTP AS A SUBSTRATE FOR TRANSCRIPTION", XP002140785, Database accession no. PREV198375056909 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1983, VAN DE WALLE C ET AL: "SPECIES OF RNA SYNTHESIZED DURING EARLY GERMINATION IN THE RADICLE OF THE LENTIL VICIA-LENS EMBRYO", XP002140784, Database accession no. PREV198376044783 *
MELVIN W T ET AL: "INCORPORATION OF 6 THIO GUANOSINE AND 4 THIO URIDINE INTO RNA APPLICATION TO ISOLATION OF NEWLY SYNTHESIZED RNA BY AFFINITY CHROMATOGRAPHY", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 92, no. 2, 1978, pages 373 - 380, XP000914888, ISSN: 0014-2956 *
PHYSIOLOGIA PLANTARUM, vol. 57, no. 2, 1983, pages 181 - 188, ISSN: 0031-9317 *
See also references of EP1144687A2 *
WOODFORD T A ET AL: "SELECTIVE ISOLATION OF NEWLY SYNTHESIZED MAMMALIAN MESSENGER RNA AFTER IN-VIVO LABELING WITH 4 THIOURIDINE OR 6 THIOGUANOSINE", ANALYTICAL BIOCHEMISTRY, vol. 171, no. 1, 1988, pages 166 - 172, XP000914891, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
WO2000042221A2 (en) 2000-07-20
CA2359814A1 (en) 2000-07-20
AU3345500A (en) 2000-08-01
JP2002534136A (en) 2002-10-15
WO2000042221A9 (en) 2001-11-08
US6960438B2 (en) 2005-11-01
US20020146739A1 (en) 2002-10-10
AU767583B2 (en) 2003-11-20
EP1144687A2 (en) 2001-10-17
US6413720B1 (en) 2002-07-02

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