WO2000037918A2 - A method and an apparatus for cutting of tissue blocks - Google Patents
A method and an apparatus for cutting of tissue blocks Download PDFInfo
- Publication number
- WO2000037918A2 WO2000037918A2 PCT/DK1999/000720 DK9900720W WO0037918A2 WO 2000037918 A2 WO2000037918 A2 WO 2000037918A2 DK 9900720 W DK9900720 W DK 9900720W WO 0037918 A2 WO0037918 A2 WO 0037918A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tissue block
- tissue
- moulding
- cutting
- mould
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Definitions
- the present invention relates to a method and an apparatus for cutting of a tissue block for pathological examination.
- the invention further relates to a method an apparatus for preparing a tissue block for sectioning in such an apparatus by use of such method.
- the invention also relates to a tissue embedding obtained by said method and apparatus for preparing a tissue block.
- the cutting of larger tissue blocks for pathological examination has normally been performed by hand.
- This technique involves a special pathology knife that is used for cutting slices of parenchymateous organs such as brain, liver, kidney and heart.
- This cutting technique is quick and sufficient for the daily qualitative examinations on a pathological institute.
- the technique result in tissue sections with highly variable form and thickness, just as the hand cutting does not prevent deformation of the organ.
- US 4,820,504 discloses a method of preparing a multi-specimen tissue block and sections thereof, where a plurality of different anitigenically reactive tissue specimens are formed into a rods and embedded in a medium and then sliced off into sections which each contain a cross-section of the rod.
- the resulting tissue slices are inaccurate in form due to possible deformation during the formation and during the slicing of a rod.
- these sections are only usable as check samples of the different tissues in the multi-specimen tissue block from which the section is sliced off.
- the present techniques for preparing tissue sections for pathological examination are in accurate and does prevent deformation of the tissue block to an acceptable degree.
- Another object of the invention is to provide for new quantitative unbiased stereological techniques which require cutting of the tissue in question in sections with equal thickness and orientation.
- tissue block in slices with a predetermined orientation in the tissue block preferably corresponding to orientation of the plane of a scanning, such as a CT, MR or PET scanning, wherein the tissue block, such as an internal organ or other internal anatomical structures, is placed with a predetermined position and then simultaneously sliced into a multiple of sections.
- a scanning such as a CT, MR or PET scanning
- the invention in a second aspect, involves an apparatus for cutting of a tissue block in slices with a predetermined orientation in the tissue block for obtaining a direct correlation of CT, MR or PET images for pathological examination, said apparatus comprising a support surface for receiving a tissue block, sectioning means comprising a multiple of cutting members, and driving means for moving the sectioning means towards the support surface for slicing a tissue block into sections.
- the invention circumvents the problems that lead to bias when quantitative organ or tissue examinations are desired.
- the invention is ideally suited for the new quantitative unbiased stereological techniques which require cutting of a tissue block in sections with equal thickness and orientation.
- the invention also allows the resulting organ or tissue sections to be directly correlated to corresponding scanning planes from imaging modalities such as e.g. computerised tomography (CT), magnetic resonance imaging (MRJ) and positron emission tomography (PET).
- CT computerised tomography
- MRJ magnetic resonance imaging
- PET positron emission tomography
- the invention involves a method and an apparatus for preparing a tissue block for sectioning in the slicing machine.
- the organ or tissue is embedded in an alginate plastic polymer mould that together with the embedded tissue subsequently can be sectioned in the tissue slicing machine, finally the invention also involves a tissue embedding prepared by the use of such method.
- the sectioning means comprise a multiple of parallel cutting members arranged in a cutting frame.
- the sections obtained by the simultaneous sectioning of the tissue block can be easily produced by lowering a frame with cutting members down to and through the underlying tissue block.
- the distance between the cutting members can be adjusted.
- sections of a predetermined thickness can be obtained.
- the tension of the cutting members can preferably also be adjusted.
- the risk of causing a deformation of the tissue during the cutting action can be adjusted.
- the cutting members are razor blades. This ensures a sharp and accurate cut without deforming the tissue block during the slicing.
- the cutting members can be wires.
- the cutting members can be wires.
- the support surface is provided with positioning means for allowing accurate positioning of a tissue block, preferably embedded in an embedding having predetermined reference surfaces. This ensures that the tissue can be positioned relative to the cutting members in such a way that the resulting sections correspond to scanning planes used in a scanning.
- the support surface is provided with vacuum supply means for retaining the tissue block in a predetermined position.
- vacuum supply means for retaining the tissue block in a predetermined position.
- centring means with a laser pointer are provided for accurate positioning of the tissue block on the support surface.
- the laser can be used for accurate position of the tissue block relative to the cutting members by assisting the positioning of the tissue block in the centre of the support surface. 5
- This positioning could also comprise concentric centring marking circles in the support surface and possibly supplemented with an aiming crossing lines. This could e.g. be in the form of concentric recesses in the support surface.
- concentric circular suction rings are provided that can be supplied with vacuum from the vacuum supply means for retaining the tissue block. This is particularly advantageous since the vacuum can be used not only for the retention but also for the aligning or centring of the tissue block.
- the cutting members are preferably connected to vibration means for vibration during the slicing action, in order to facilitate the cutting action and prevent deformation of the tissue during the cutting action.
- the vibration means could advantageously comprise a pneumatic vibrator that is 20 connected to pneumatic supply means.
- the vacuum in the vacuum supply means could preferably be generated by vacuum generating means connected to the pneumatic supply means.
- vacuum generating means connected to the pneumatic supply means.
- the driving means comprise pillar guiding means provided on the support surface and linear actuation means for linear movement of the sectioning means towards the support surface along the path defined by the pillar guiding means. This allows an accurate and smooth linear movement of the cutting
- the linear actuation means preferably comprise a threaded driving spindle parallel to the guide means and a corresponding threading in the cutting frame.
- the threaded driving spindle is provided with a handle for manual operation. This offers a simple apparatus for carrying out the sectioning.
- the driving spindle can be pneumatically or electrically driven.
- the invention also relates to method and an apparatus for preparing a tissue block.
- This method comprises the steps of filling a moulding form with an appropriate amount of non-toxic, biologically inert polymer moulding material, said form having at least one reference surface, and positioning a tissue block in said polymer moulding material in a predetermined position relative to said at least one reference surface, while the polymer moulding material is in its soft state.
- the tissue block is provided with regular outer surfaces that due to the form of the mould can adapted to the support surface of the sectioning apparatus.
- the tissue block is in a preferred embodiment positioned in the polymer material with an orientation that corresponds to the orientation of the tissue block in vivo.
- the tissue block is embedded in a bottom mould part and a top mould is formed in a top moulding form that is filled with polymer moulding material and placed on the top of the lower moulding part with a partly encased tissue block, so that the tissue block is completely encased by in the moulding.
- This provides an effective insurance against the otherwise free top part of the tissue to be deformed by the cutting members.
- the tissue block is in a preferred method of preparation fixed to a reference moulding of predetermined dimensioned and that said reference moulding is pivoted into a predetermined position in one or more directions, and then moulded into at least a bottom moulding.
- the orientation of the tissue block can be vary accurately embedded relative to the reference surfaces.
- the polymer material that is preferably used is a cold polymerisate that polymerises by addition of water, such as an algino plastic polymer.
- the invention also relates to a tissue embedding comprising a tissue block made by this preparation method and apparatus.
- This tissue embedding providing the tissue block with regular reference surfaces ensures an accurate cutting of slices of the block for pathological and other purposes. It is realised that this technique of embedding the tissue block in an alginate or similar suitable moulding material can advantageously be used prior to any cutting action, whether a slice at the time is cut or - as it is the case in the first aspect of the invention - that the slices are cut simultaneously.
- Fig. 1 is a perspective view of an apparatus for sectioning a tissue block according to the invention
- fig. 2 shows a cutting frame of said apparatus
- fig. 3a and 3b show the tissue block embedded in an alginate bottom and with an alginate top mould
- fig. 4a to 4g show the embedding apparatus and the steps in the oriented alginate embedding procedure
- fig. 5 is a top view of the embedding apparatus of figs. 4a-4g.
- fig. 1 a preferred embodiment of the tissue slicing machine is shown.
- the tissue slicing machine stand on an aluminium or steel base plate 1 that preferably rest on a rubber pad or rubber knobs attached to the base plate 1.
- the base plate 1 is connected to the aluminium or steel top plate 2 by pillar guiding means comprising two the pillars 3 which through operation of the crank and spindle 8, 9 allow lowering and elevation of the top plate 2 in relation to the base plate 1.
- pillar guiding means comprising two the pillars 3 which through operation of the crank and spindle 8, 9 allow lowering and elevation of the top plate 2 in relation to the base plate 1.
- the cutting frame 12 is fixed in place by screws or a handle on the side of the cutting frame 12.
- the cutting frame 12 comprises a number of cutting members 14 (see fig. 2), preferably in the form of thin razor blades of hardened steel.
- the razor blades 14 are spaced by spacing blocks 38 that can be made of metal or plastic.
- the cutting frame 12 is exchangeable as a whole when the razor blades 12 are worn out and has lost their sharpness.
- the knife frame 12 allow changing or removal of individual blades 14, just as spacing blocks 38 of different thickness can be used.
- a pneumatic or electric operated vibrator 4 is placed on the side of the top plate 2 . It will when activated set the sectioning means comprising the top plate 2 and knife frame 12 into vibrations along the long axis of the razor blades 14. This facilitates the cutting procedure by lowering of the friction as the knives 14 pass through the tissue 20 and alginate block 25 (see figs. 3 a and 3b).
- a pneumatic valve 7 for pressurised air to operate the embodiment with the pneumatic vibrator 4.
- the pneumatic vibrator 4 is connected to the pneumatic air valve 7 through a pneumatic hose 5.
- the pneumatic air valve 7 is connected to a pressurised air source at the pneumatic intake 6.
- a valve 13 for vacuum with vacuum outtake 10 is placed on the other side of the top plate 2 or on the base plate 1 in another embodiment.
- the vacuum outtake 10 is connected to a vacuum pump (not shown).
- the vacuum is produced by a second pneumatic air flow valve (not shown).
- the vacuum hose 15 connects the vacuum valve 13 to a recess and associated apertures 16 for retention and vacuum fixation of alginate and tissue block 25.
- concentric circles 17 and a cross hair cut allow centring of alginate and tissue block 25.
- a laser pointer 11 that point to the central vacuum hole of the vacuum apertures 16, is provided on the top plate.
- Fig. 2 show an embodiment of the cutting frame 12 consisting of knives 14 that are angled in relation to the horizontal plane of the base plate 1. This embodiment will reduce friction and deformation during the cutting of the tissue alginate block 25.
- the knife frame 12 consist of razor blades 14 that cannot be replaced, and the whole frame 12 must be changed when the blades become dull.
- the knife frame allow exchanging of individual blades and use of spacing blocks 38 with different thickness.
- the bearing construction of the tissue slicing machine comprises a pillar guided base and top plate 1 and 2.
- the top plate 2 contains a set of parallel oriented knives 14 positioned in a frame 12.
- the knives 14 are mounted in a "knife frame set" and the distance between the knives 14 are spaced by high tolerance spacing blocks 38 with an equal thickness. Changing between different knife frame sets can vary the knives distance.
- the frame of the tissue slicing machine is equipped with a pneumatic vibrator 4 that make the knife frame set vibrate along its longitudinal axis, i.e. along the cutting edge of the knives.
- the vibration 4 of the knife frame 12 diminishes friction as it moves through alginate and tissue block 25.
- the knife frame with vibrator is mounted on a columnar lead equipped with a crank 19 that by turning allow movement of the knife frame 12 in the vertical plane.
- the base plate 1 of the tissue slicing machine is equipped with a suction pad that is activated by opening a vacuum valve after placement of the alginate tissue block.
- the concentric rings of the suction pad also serve to centre the alginate tissue block, just as a laser pointer identifies the centre.
- any tissue block 20 or organ can be embedded into an alginate plastic mould 25.
- the tissue 20 is first embedded in an alginate bottom mould 22. This can be done by pouring the mixture of alginate powder and water in to a moulding form 21, such as a plastic jar, followed by placement of the tissue 20 into the still soft alginate-water mixture in the mould 22.
- a moulding form 21 such as a plastic jar
- an alginate top mould 23 can be cast in a similar fashion by placing a second moulding form 24, such as a second plastic jar 24.
- This top mould 23 can subsequently be removed for better placement of the alginate bottom 22 in the tissue slicing machine as shown in fig. 1 by then use of anatomical landmarks.
- the tissue 20 can be cast entirely into alginate followed by CT or MRI scanning of the tissue and alginate block.
- CT or MRI scanning of the tissue and alginate block.
- the resulting tissue sections will correspond to the scanning planes.
- alginate embedded tissue can be cut on prior art tissue sectioning machines, such as cryostats, vibratomes and microtomes.
- alginate plastic polymer from Bayer Dental was used.
- the alginate is a non-toxic cold polymerisate that polymerises after addition of water.
- the alginate powder is stirred into the water and then poured into a plastic jar or other moulding form 21 of appropriate size for the tissue block in question.
- the organ or tissue block 20, such as a pig brain, is then placed in the still soft polymer and hold in place until the alginate hardens.
- the embedding is the crucial step in the process and care must be taken to orient the tissue 20 in the alginate as it is oriented in vivo. For less accuracy this can achieved by the use of an angle protractor 34-36 and anatomical landmarks on the tissue 20 in question.
- tissue embedder For high accuracy the tissue embedder must be used.
- a further option is to cast another alginate mould 24on top of the tissue and alginate bottom 22. This done in order to support the tissue 20 during the cutting procedure and avoid tissue deformation. In the following this will be described as a tissue and alginate bottom 22 and an alginate lid 24.
- An alternative strategy that can be used, if no scanning is needed before pathological extraction of the organ, is to embed the organ 20 in alginate and then perform the desired computer assisted scanning modality on tissue and alginate block 25 followed by the sectioning as described by the first aspects of the present invention.
- This strategy abolishes the need for orientation of the tissue block 20 as the resulting digital image scanning planes will correspond to the histological sections provided that the tissue and alginate block 25 is placed in the cutting machine in the same fashion as in the CT, MRI or PET scanner.
- the tissue block 25 with the embedded tissue 20 is placed on the suction pads 16 of the support surface and centred in relation to the cutting frame 12 by use of the concentric circles 17 of the tissue slicing machine base plate 1 and the laser pointer 11.
- the alginate tissue block 25 is fixed by activation of the vacuum valve 13 and is now ready for the cutting. This can be done with or without the alginate lid 24.
- An opening of the pneumatic valve 7 activates the pneumatic vibrator 4 and the cutting frame 12 starts vibrating.
- the crank 19 of the columnar lead is turned and the cutting frame 12 is lowered through the alginate and tissue block 25.
- the cutting results in a set of alginate and tissue slabs (not shown) that are of equal thickness and oriented corresponding to the scanning plane of the given computerised scanning modality.
- FIG. 4a to 4g a method and an apparatus for preparing a tissue block 20 by embedding the tissue block 20 in alginate plastic polymer. This can be done in such a way that the tissue 20 is oriented to existing CT, MRI or PET scanning planes.
- Fig. 4a shows the embedding apparatus that comprises of a central plastic rod 28 with a spheric top end 28a and two concentric plastic cylinders, i.e. an inner cylinder 29 and an outer cylinder 30.
- the rod 28 is fixed to a plastic base plate 31, where upon also the cylinders 29, 30 rest in their retracted positions.
- On top of the outer cylinder 30 four plastic pins 33 with or without a screw thread are placed at 90 degrees interval and orthogonal to the long axis of the cylinder 30 (see fig. 5).
- a reference moulding form 27 is placed on plate means 32 comprising two half parts placed on the inner cylinder 29 on each side of the rod 28.
- a reference moulding form is defined.
- This form is filled with polymer moulding material 26 in which the tissue block 20 is placed. This means that the tissue block 20 is embedded in a reference mould 26, such as shown in fig. 4b, where the plate means 32 and the form 27 is removed.
- Fig. 4c shows a tissue and alginate reference mould 26 placed in the embedding apparatus.
- the alginate mould 26 is first fixed with two plastic pins 33 facing each other at 180 degrees. This plane can be defined as the X plane.
- the tilt angle of the tissue and alginate reference mould 26 in relation to the horizontal Z plane, as determined from the desired CT, MRI or PET scanning, is determined by an angle protractor 36 (see fig. 4d) in one embodiment.
- the angle protractor 36 is equipped with a laser guide 34 directing a beam 35 at the mould for a measure of the angle of inclination.
- Fig. 4e shows the casting of the second alginate bottom 22.
- the shape of the bottom mould 22 is adapted to fit into the tissue slicing machine of fig. 1.
- the outer cylinder 30 is raised and a second base plate 37 is slid into a corresponding horizontal opening in the outer plastic cylinder 30, followed by casting of the second alginate bottom 22, as the outer cylinder 30 forms the side part of the moulding form.
- Fig. 4f shows the placement of a top form 21 on the outer cylinder 30 followed by casting of an alginate top mould 24 on the top of the bottom mould 22 and the tissue block 20.
- Fig. 4g shows the embedded tissue block 20 with its alginate moulding 25 - i.e. the reference mould 26, bottom mould 22 and top mould 24 - free of the embedding apparatus after the outer cylinder 30 has been slid back towards the base plate 31.
- Fig. 5 shows a top view of the outer cylinder 30, inner cylinder 29, centre rod 28 and plastic pins 33 for pivoting the reference mould 26 of the tissue block.
- This embedding apparatus allows accurate three dimensional orientation of a tissue reference moulding 26 in relation to CT, MRI or PET scans.
- a second moulding is performed to produce a second alginate bottom 22 with an outer surface that will fit into the tissue slicing machine.
- a final alginate lid 24 can be cast on top of the tissue 20 and alginate bottom 22 in order to avoid tissue deformations during the cutting procedure.
- the embedding apparatus comprises a circular rod 29 of transparent plastic, such as plexi-glass or similar material, two outer concentric plastic cylinders 29 and 30, fixation pins 33, semicircular plastic plates32 and an insertable base plate 37 that preferably also is made from a plastic material.
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020017007792A KR20010099854A (en) | 1998-12-21 | 1999-12-20 | A method and an apparatus for cutting of tissue blocks |
EP99960950A EP1149275A2 (en) | 1998-12-21 | 1999-12-20 | A method and an apparatus for cutting of tissue blocks |
CA002353876A CA2353876A1 (en) | 1998-12-21 | 1999-12-20 | A method and an apparatus for cutting of tissue blocks |
JP2000589928A JP2002533670A (en) | 1998-12-21 | 1999-12-20 | Method and apparatus for cutting tissue mass |
AU17734/00A AU1773400A (en) | 1998-12-21 | 1999-12-20 | A method and an apparatus for cutting of tissue blocks |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA199801699 | 1998-12-21 | ||
DKPA199801699 | 1998-12-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2000037918A2 true WO2000037918A2 (en) | 2000-06-29 |
WO2000037918A3 WO2000037918A3 (en) | 2001-03-08 |
Family
ID=8107314
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK1999/000720 WO2000037918A2 (en) | 1998-12-21 | 1999-12-20 | A method and an apparatus for cutting of tissue blocks |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1149275A2 (en) |
JP (1) | JP2002533670A (en) |
KR (1) | KR20010099854A (en) |
AU (1) | AU1773400A (en) |
CA (1) | CA2353876A1 (en) |
WO (1) | WO2000037918A2 (en) |
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WO2002037080A1 (en) * | 2000-11-03 | 2002-05-10 | Histotech Aps | A device and a method for sectioning of tissue blocks |
DE10311061A1 (en) * | 2003-03-13 | 2004-09-30 | Cell Center Cologne Gmbh | Apparatus to cut a tissue sample into thin slices, for analysis, has a casting frame to embed the sample in a cast block, to be advanced to a lateral cutting blade to cut slices in a selected thickness |
EP2128594A2 (en) | 2008-05-30 | 2009-12-02 | Shu Ichihara | Specimen slicing guide, specimen slicing apparatus and method of using the same |
CN102362168A (en) * | 2009-03-27 | 2012-02-22 | 索尼公司 | Observation device |
US8122804B2 (en) | 2006-11-01 | 2012-02-28 | Kurume University | Slicing guide device for preparing texture slices, texture slices preparing device, and method for preparing texture slices |
DE102012214476A1 (en) * | 2012-08-14 | 2014-02-20 | Leica Microsystems Cms Gmbh | Apparatus and method for producing a plurality of sample cuts in the form of a cut stack |
US9097619B2 (en) | 2011-04-27 | 2015-08-04 | Shu Ichihara | Specimen slicing guide |
WO2017009800A1 (en) | 2015-07-16 | 2017-01-19 | Ecole Polytechnique Federale De Lausanne (Epfl) | Device for high- throughput sample slicing and characterization |
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CN108318310A (en) * | 2018-05-14 | 2018-07-24 | 上海交通大学医学院附属第九人民医院 | A kind of lower device for the parallel irregular biological tissue of cutting of laser scanning guiding |
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US10281534B2 (en) | 2014-03-20 | 2019-05-07 | Life Services, LLC | Tissue-slice MRI coil and rotation mechanism |
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US10324146B2 (en) | 2016-01-12 | 2019-06-18 | Life Services, LLC | Method and apparatus for multi-part body coil |
US10827948B1 (en) | 2015-11-25 | 2020-11-10 | Life Services, LLC | Method and apparatus for multi-part close fitting head coil |
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JP5881006B2 (en) * | 2011-12-27 | 2016-03-09 | 東芝Itコントロールシステム株式会社 | Tomography equipment |
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JP7195339B2 (en) * | 2018-12-17 | 2022-12-23 | ヤマハ発動機株式会社 | Biological sample slicer |
KR102576409B1 (en) | 2021-01-18 | 2023-09-08 | 충남대학교병원 | Method for preparing cell-section using alginate bead |
KR102526386B1 (en) | 2021-03-19 | 2023-05-02 | 충남대학교병원 | Method for cell block production using alginate-PVA composite beads |
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- 1999-12-20 AU AU17734/00A patent/AU1773400A/en not_active Abandoned
- 1999-12-20 WO PCT/DK1999/000720 patent/WO2000037918A2/en not_active Application Discontinuation
- 1999-12-20 CA CA002353876A patent/CA2353876A1/en not_active Abandoned
- 1999-12-20 KR KR1020017007792A patent/KR20010099854A/en not_active Application Discontinuation
- 1999-12-20 JP JP2000589928A patent/JP2002533670A/en active Pending
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Cited By (20)
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---|---|---|---|---|
WO2002037080A1 (en) * | 2000-11-03 | 2002-05-10 | Histotech Aps | A device and a method for sectioning of tissue blocks |
DE10311061A1 (en) * | 2003-03-13 | 2004-09-30 | Cell Center Cologne Gmbh | Apparatus to cut a tissue sample into thin slices, for analysis, has a casting frame to embed the sample in a cast block, to be advanced to a lateral cutting blade to cut slices in a selected thickness |
US8122804B2 (en) | 2006-11-01 | 2012-02-28 | Kurume University | Slicing guide device for preparing texture slices, texture slices preparing device, and method for preparing texture slices |
EP2128594A2 (en) | 2008-05-30 | 2009-12-02 | Shu Ichihara | Specimen slicing guide, specimen slicing apparatus and method of using the same |
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Also Published As
Publication number | Publication date |
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WO2000037918A3 (en) | 2001-03-08 |
JP2002533670A (en) | 2002-10-08 |
CA2353876A1 (en) | 2000-06-29 |
KR20010099854A (en) | 2001-11-09 |
EP1149275A2 (en) | 2001-10-31 |
AU1773400A (en) | 2000-07-12 |
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