WO2000037656A3 - Enzymes biosynthetiques flavonoides - Google Patents

Enzymes biosynthetiques flavonoides Download PDF

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Publication number
WO2000037656A3
WO2000037656A3 PCT/US1999/030338 US9930338W WO0037656A3 WO 2000037656 A3 WO2000037656 A3 WO 2000037656A3 US 9930338 W US9930338 W US 9930338W WO 0037656 A3 WO0037656 A3 WO 0037656A3
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WO
WIPO (PCT)
Prior art keywords
flavonoid biosynthetic
biosynthetic enzyme
biosynthetic enzymes
flavonoid
relates
Prior art date
Application number
PCT/US1999/030338
Other languages
English (en)
Other versions
WO2000037656A2 (fr
Inventor
Omolayo O Famodu
Brian Mcgonigle
Joan T Odell
Gary M Fader
Saverio Carl Falco
Original Assignee
Du Pont
Omolayo O Famodu
Brian Mcgonigle
Joan T Odell
Gary M Fader
Saverio Carl Falco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Omolayo O Famodu, Brian Mcgonigle, Joan T Odell, Gary M Fader, Saverio Carl Falco filed Critical Du Pont
Priority to AU21992/00A priority Critical patent/AU2199200A/en
Publication of WO2000037656A2 publication Critical patent/WO2000037656A2/fr
Publication of WO2000037656A3 publication Critical patent/WO2000037656A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne un fragment d'acide nucléique isolé codant une enzyme biosynthétique flavonoïde. En outre, cette invention concerne la synthèse d'un gène chimère codant tout ou partie de l'enzyme biosynthétique flavonoïde, dans une orientation sens ou antisens; l'expression du gène chimère ayant pour effet de produire des taux modifiés de l'enzyme biosynthétique flavonoïde dans une cellule hôte transformée.
PCT/US1999/030338 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides WO2000037656A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU21992/00A AU2199200A (en) 1998-12-21 1999-12-20 Flavonoid biosynthetic enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11319098P 1998-12-21 1998-12-21
US60/113,190 1998-12-21

Publications (2)

Publication Number Publication Date
WO2000037656A2 WO2000037656A2 (fr) 2000-06-29
WO2000037656A3 true WO2000037656A3 (fr) 2000-09-14

Family

ID=22348054

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1999/030337 WO2000037652A2 (fr) 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides
PCT/US1999/030338 WO2000037656A2 (fr) 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US1999/030337 WO2000037652A2 (fr) 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides

Country Status (2)

Country Link
AU (2) AU2199200A (fr)
WO (2) WO2000037652A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004500803A (ja) * 1999-05-20 2004-01-15 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク イソフラボノイドメチル化酵素
US6878859B1 (en) 1999-05-20 2005-04-12 The Samuel Roberts Noble Foundation, Inc. Isoflavonoid methylation enzyme
EP1515600B1 (fr) 2002-06-14 2015-08-12 Syngenta Participations AG Tomate cultivee exprimant des flavonols et son procede de production
US7442851B2 (en) 2003-10-29 2008-10-28 The Samuel Roberts Noble Foundation Plant isoflavonoid hydroxylases and methods of use thereof
PL1750760T3 (pl) 2004-06-02 2018-02-28 Universal Stabilization Technologies, Inc. Konserwacja za pomocą parowania
KR101079292B1 (ko) 2008-08-28 2011-11-04 건국대학교 산학협력단 생물학적 변환을 통해 제니스테인을 2-하이드록시 제니스테인으로 전환시키는 방법

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 1977, vol. 180, no. 2, 1977, pages 543 - 549, ISSN: 0003-9861 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1977, POULTON J E ET AL: "O METHYLATION OF FLAVONOID SUBSTRATES BY A PARTIALLY PURIFIED ENZYME FROM SOYBEAN CELL SUSPENSION CULTURES", XP002139381, Database accession no. PREV197764047255 *
HE X.Z., ET AL.: "Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete cds.", EMBL ACCESSION NO:AF000976, 5 November 1997 (1997-11-05), XP002139379 *
HE X.Z., ET AL.: "Medicago sativa isoflavone-O-methytransferase mRNA, complete cds.", EMBL ACCESSION NO:U97125, 5 November 1997 (1997-11-05), XP002139380 *
HE XIAN-ZHI ET AL: "Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-0-methyltransferase.", PLANT MOLECULAR BIOLOGY JAN. 1, 1998, vol. 36, no. 1, 1 January 1998 (1998-01-01), pages 43 - 54, XP002139377, ISSN: 0167-4412 *
HE, X.Z., ET AL.: "Medicago sativa 7-O-methyltransferase (7-IOMT(6)) mRNA, complete cds.", EMBL ACCESSION NO:AF000975, 5 November 1997 (1997-11-05), XP002139378 *
SHOEMAKER R., ET AL.: "sa87h11.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID:Gm-c1004-6334 5' similar to TR:O22308 O22308 7-O-METHYLTRANSFERASE.[3] TR:O22309 TR:O22555 ;, mRNA sequence.", EMBL ACCESSION NO:AI507849, 17 March 1999 (1999-03-17), XP002139373 *
SHOEMAKER, R., ET AL.: "sa82g01.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID:Gm-c1004-5833 5' similar to SW:ZRP4_MAIZE P47917O-METHYLTRANSFERASE ZRP4 ;, mRNA sequence.", EMBL ACCESSION NO:AI441988, 16 March 1999 (1999-03-16), XP002139376 *
SHOEMAKER, R., ET AL.: "sa88b04.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID:Gm-c1004-6344 5' similar to TR:O22308 O22308 7-O-METHYLTRANSFERASE.[3] TR:O22309 TR:O22555 ;, mRNA sequence.", EMBL ACCESSION NO:AI507864, 17 March 1999 (1999-03-17), XP002139375 *
SHOEMAKER, R., ET AL.: "sf38a06.y1 Gm-c1028 Glycine max cDNA clone GENOME SYSTEMS CLONE ID:Gm-c1028-2219 5' similar to TR:O22555 O22555 O-METHYTRANSFERASE.[2] TR:O22308 ;, mRNA sequence.", EMBL ACCESSION NO:AW203767, 10 December 1999 (1999-12-10), XP002139374 *

Also Published As

Publication number Publication date
WO2000037656A2 (fr) 2000-06-29
AU2199100A (en) 2000-07-12
AU2199200A (en) 2000-07-12
WO2000037652A2 (fr) 2000-06-29
WO2000037652A3 (fr) 2000-11-16

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