WO2000037652A3 - Enzymes biosynthetiques flavonoides - Google Patents

Enzymes biosynthetiques flavonoides Download PDF

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Publication number
WO2000037652A3
WO2000037652A3 PCT/US1999/030337 US9930337W WO0037652A3 WO 2000037652 A3 WO2000037652 A3 WO 2000037652A3 US 9930337 W US9930337 W US 9930337W WO 0037652 A3 WO0037652 A3 WO 0037652A3
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WO
WIPO (PCT)
Prior art keywords
flavonoid biosynthetic
biosynthetic enzyme
biosynthetic enzymes
flavonoid
relates
Prior art date
Application number
PCT/US1999/030337
Other languages
English (en)
Other versions
WO2000037652A2 (fr
Inventor
Omolayo O Famodu
Brian Mcgonigle
Joan T Odell
Gary M Fader
Saverio Carl Falco
Original Assignee
Du Pont
Omolayo O Famodu
Brian Mcgonigle
Joan T Odell
Gary M Fader
Saverio Carl Falco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Omolayo O Famodu, Brian Mcgonigle, Joan T Odell, Gary M Fader, Saverio Carl Falco filed Critical Du Pont
Priority to AU21991/00A priority Critical patent/AU2199100A/en
Publication of WO2000037652A2 publication Critical patent/WO2000037652A2/fr
Publication of WO2000037652A3 publication Critical patent/WO2000037652A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne un fragment d'acide nucléique isolé codant une enzyme biosynthétique flavonoïde. En outre, cette invention concerne la synthèse d'un gène chimère codant tout ou partie de l'enzyme biosynthétique flavonoïde, dans une orientation sens ou antisens; l'expression du gène chimère ayant pour effet de produire des taux modifiés de l'enzyme biosynthétique flavonoïde dans une cellule hôte transformée.
PCT/US1999/030337 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides WO2000037652A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU21991/00A AU2199100A (en) 1998-12-21 1999-12-20 Flavonoid biosynthetic enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11319098P 1998-12-21 1998-12-21
US60/113,190 1998-12-21

Publications (2)

Publication Number Publication Date
WO2000037652A2 WO2000037652A2 (fr) 2000-06-29
WO2000037652A3 true WO2000037652A3 (fr) 2000-11-16

Family

ID=22348054

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1999/030338 WO2000037656A2 (fr) 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides
PCT/US1999/030337 WO2000037652A2 (fr) 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US1999/030338 WO2000037656A2 (fr) 1998-12-21 1999-12-20 Enzymes biosynthetiques flavonoides

Country Status (2)

Country Link
AU (2) AU2199100A (fr)
WO (2) WO2000037656A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004500803A (ja) * 1999-05-20 2004-01-15 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク イソフラボノイドメチル化酵素
US6878859B1 (en) 1999-05-20 2005-04-12 The Samuel Roberts Noble Foundation, Inc. Isoflavonoid methylation enzyme
US7851680B2 (en) 2002-06-14 2010-12-14 Syngenta Participations Ag Flavonol expressing domesticated tomato and method of production
WO2005042747A2 (fr) * 2003-10-29 2005-05-12 The Samuel Roberts Noble Foundation, Inc. Hydroxylases d'isoflavonoides de plantes et leurs procedes d'utilisation
CA2569276C (fr) 2004-06-02 2018-01-23 Victor Bronshtein Conservation par vaporisation
KR101079292B1 (ko) 2008-08-28 2011-11-04 건국대학교 산학협력단 생물학적 변환을 통해 제니스테인을 2-하이드록시 제니스테인으로 전환시키는 방법

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
AKASHI TOMOYOSHI ET AL: "CYHP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS OCT. 9, 1998, vol. 251, no. 1, 9 October 1998 (1998-10-09), pages 67 - 70, XP000914524, ISSN: 0006-291X *
AKASHI, T., ET AL.: "cloning of cytochrome P450 cDNAs from cultured Glycyrrhiza echinata L. cells and their transcriptional activation by elicitor-treatment", EMBL SEQUENCE DATA LIBRARY, 23 September 1997 (1997-09-23), heidelberg, germany, XP002139419 *
CLEMENS STEPHAN ET AL: "Characterization of cytochrome P450-dependent isoflavone hydroxylases from chickpea.", PHYTOCHEMISTRY (OXFORD) 1993, vol. 32, no. 3, 1993, pages 653 - 657, XP000920560, ISSN: 0031-9422 *
DANIEL, S., ET AL.: "elicitor-induced metabolic changes in cel cultures of chickpea (Cicer arietinum L.) cultivars resistant and susceptible to Ascochyta rabiei. I. investigations of enzyme activities involved in isoflavone and pterocarpan phytoalexin biosynthesis", PLANTA, vol. 182, no. 2, 1990, pages 270 - 278, XP000914222 *
HINDERER W ET AL: "MICROSOMAL ISOFLAVONE 2' AND 3'-HYDROXYLASES FROM CHICKPEA CICER-ARIETINUM L. CELL SUSPENSIONS INDUCED FOR PTEROCARPAN PHYTOALEXIN FORMATION", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) LETTERS 1987, vol. 214, no. 1, 1987, pages 101 - 106, XP002139422, ISSN: 0014-5793 *
KESSMANN H ET AL: "STRESS RESPONSES IN ALFALFA MEDICAGO-SATIVA L. V. CONSTITUTIVE AND ELICITOR-INDUCED ACCUMULATION OF ISOFLAVONOID CONJUGATES IN CELL SUSPENSION CULTURES", PLANT PHYSIOLOGY (BETHESDA) 1990, vol. 94, no. 1, 1990, pages 227 - 232, XP002139423, ISSN: 0032-0889 *
OVERKAMP, S. AND BARZ, W.: "cloning of two Cicer arietinum L. cDNAs encoding cytochrome P450 highly homologous to isoflavone 2' hydroxylase from licorice", EMBL SEQUENCE DATA LIBRARY, 6 November 1998 (1998-11-06), heidelberg, germany, XP002139420 *
SHOEMAKER, R., ET AL.: "public soybean EST project", EMBL SEQUENCE DATA LIBRARY, 15 December 1999 (1999-12-15), heidelberg, germany, XP002139424 *

Also Published As

Publication number Publication date
WO2000037656A3 (fr) 2000-09-14
AU2199200A (en) 2000-07-12
WO2000037652A2 (fr) 2000-06-29
AU2199100A (en) 2000-07-12
WO2000037656A2 (fr) 2000-06-29

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