WO2000032790A3 - Desaturases liees a la membrane - Google Patents

Desaturases liees a la membrane Download PDF

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Publication number
WO2000032790A3
WO2000032790A3 PCT/US1999/028589 US9928589W WO0032790A3 WO 2000032790 A3 WO2000032790 A3 WO 2000032790A3 US 9928589 W US9928589 W US 9928589W WO 0032790 A3 WO0032790 A3 WO 0032790A3
Authority
WO
WIPO (PCT)
Prior art keywords
desaturase
delta
sphingolipid
membrane
relates
Prior art date
Application number
PCT/US1999/028589
Other languages
English (en)
Other versions
WO2000032790A2 (fr
Inventor
Edgar B Cahoon
Rebecca E Cahoon
William D Hitz
Anthony J Kinney
Original Assignee
Du Pont
Edgar B Cahoon
Rebecca E Cahoon
William D Hitz
Anthony J Kinney
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Edgar B Cahoon, Rebecca E Cahoon, William D Hitz, Anthony J Kinney filed Critical Du Pont
Priority to AU21631/00A priority Critical patent/AU2163100A/en
Priority to US09/857,524 priority patent/US6864077B1/en
Publication of WO2000032790A2 publication Critical patent/WO2000032790A2/fr
Publication of WO2000032790A3 publication Critical patent/WO2000032790A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne un fragment isolé d'acide nucléique codant pour une delta-6 désaturase ou une désaturase de sphingolipide. L'invention concerne aussi la construction d'un gène chimère codant pour l'intégralité ou une partie de delta-6 désaturase ou de désaturase de sphingolipide, à orientation sens ou antisens, l'expression du gène chimère menant à la production de quantités modifiées de delta-6 désaturase ou de désaturase de sphingolipide dans une cellule hôte transformée.
PCT/US1999/028589 1998-12-03 1999-12-02 Desaturases liees a la membrane WO2000032790A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU21631/00A AU2163100A (en) 1998-12-03 1999-12-02 Membrane-bound desaturases
US09/857,524 US6864077B1 (en) 1998-12-03 1999-12-02 Membrane-bound desaturases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11078498P 1998-12-03 1998-12-03
US60/110,784 1998-12-03

Publications (2)

Publication Number Publication Date
WO2000032790A2 WO2000032790A2 (fr) 2000-06-08
WO2000032790A3 true WO2000032790A3 (fr) 2000-11-16

Family

ID=22334913

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/028589 WO2000032790A2 (fr) 1998-12-03 1999-12-02 Desaturases liees a la membrane

Country Status (3)

Country Link
AR (1) AR021544A1 (fr)
AU (1) AU2163100A (fr)
WO (1) WO2000032790A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3086291A1 (fr) * 2017-12-22 2019-06-27 Basf Plant Science Company Gmbh Procede et moyens d'isolement de proteines liees a une membrane a partir d'un echantillon biologique, de preference une farine de graine de plante traitee

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021022A2 (fr) * 1994-12-30 1996-07-11 Rhone-Poulenc Agrochimie Production d'acide linoleique gamma au moyen d'une δ6-desaturase
WO1998045461A1 (fr) * 1997-04-09 1998-10-15 Rhone-Poulenc Agro Region regulatrice 5' d'oleosine servant a la modification de la composition lipidique de graines vegetales

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996021022A2 (fr) * 1994-12-30 1996-07-11 Rhone-Poulenc Agrochimie Production d'acide linoleique gamma au moyen d'une δ6-desaturase
WO1998045461A1 (fr) * 1997-04-09 1998-10-15 Rhone-Poulenc Agro Region regulatrice 5' d'oleosine servant a la modification de la composition lipidique de graines vegetales

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DELHAIZE ET AL.: "S276", EMBL SEQUENCE DATABASE, 1 May 1999 (1999-05-01), HEIDELBERG DE, XP002145315 *
DELHAIZE ET AL.: "Triticumaestivium S276) (S276) mRNA, complete cds", EMBL SEQUENCE DATABASE, 6 January 1999 (1999-01-06), HEIDELBERG DE, XP002145280 *
OLGA SAYANOVA ET AL: "Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of hig levels of Delta6-desaturated fatty acids in transgenic tobacco", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 94, 15 April 1997 (1997-04-15), pages 4211 - 4216, XP002106758, ISSN: 0027-8424 *
SHEN ET AL.: "Putative cytochrome B5", EMBL SEQUENCE DATABASE, 1 November 1998 (1998-11-01), HEIDELBERG DE, XP002145279 *
SPERLING ET AL: "A sphingolipid desaturase from higher plants. Identification of a new cytochrome b5 fusion protein", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 273, no. 44, 30 October 1998 (1998-10-30), pages 28590 - 28596, XP002132572, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
AR021544A1 (es) 2002-07-24
AU2163100A (en) 2000-06-19
WO2000032790A2 (fr) 2000-06-08

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