WO2000029426A1 - Compositions and methods for controlling plant pests - Google Patents
Compositions and methods for controlling plant pests Download PDFInfo
- Publication number
- WO2000029426A1 WO2000029426A1 PCT/US1999/024827 US9924827W WO0029426A1 WO 2000029426 A1 WO2000029426 A1 WO 2000029426A1 US 9924827 W US9924827 W US 9924827W WO 0029426 A1 WO0029426 A1 WO 0029426A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- strain
- plants
- insect infestations
- novel
- effective amount
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/832—Bacillus
- Y10S435/839—Bacillus subtilis
Definitions
- the present invention is in the field of biopesticides. More particularly, this invention relates to the finding that a novel strain o ⁇ Bacillus subtilis, AQ713, can inhibit a broad range of fungal and bacterial plant diseases and also have activity against insects. The invention also relates to fungicidal, bactericidal, and insecticidal compositions comprising this novel Bacillus strain and the antibiotics and metabolites produced by this strain either alone, or in combination with other chemical and biological pesticides.
- PHYTOPHTHORA INFESTANS HE CAUSE OF LATE BLIGHT OF POTATO (Academic Press, San Diego 1991).
- Biopesticides living organisms and the naturally produced compounds produced by these organisms
- Biopesticides developed from microorganisms are highly desired for integrated pest management programs in agriculture, public health and urban settings.
- One commonly used biopesticide is the gram positive bacterium Bacillus thuringiensis.
- Pesticidal B. thuringiensis strains are known to produce crystal proteins during sporulation, which are specifically toxic to certain orders and species of insects and nematodes (See, e.g., U.S. Patent No. 4,999,192 and U.S. Patent No. 5,208,017). Proteinaceous endotoxins produced by B.
- thuringiensis also act as insecticidal agents against corn rootworm and other beetles (see e.g., U.S. Patent No. 5,187,091; Johnson, TJ. et al. (1993), J. Economic Entomology, 86:330-333).
- B. thuringiensis endotoxins have been shown to be effective as purified crystals, washed cell pellets, and expressed proteins.
- Warren et al. disclose non- endotoxin proteins produced during the vegetative stage o ⁇ Bacillus cereus and B. thuringiensis. These vegetative proteins, called Vipl and Vip2 have potent activity against corn rootworm (northern and western). Estruch et al.
- beta-exotoxin One B. thuringiensis thermostable metabolite, termed beta-exotoxin has also been shown to have pesticidal properties. Burgjeron and Biache (1979), Entomophaga 11:279-284 report a beta exotoxin that is active against Colorado potato beetle (Leptinotarsa decemlineata). In addition, the known B. thuringiensis beta-exotoxins exhibits non-specific pesticidal activity, killing not only nematodes, but also flies, armyworm, mites, and corn rootworm.
- Sigma exotoxin has structure similar to beta-exotoxin, and is active against Colorado potato beetle (Argauer et al. (1991) J. Entomol. Sci. 26:206-213 ⁇ ).
- Alpha-exotoxin is toxic against larvae of Musca domestica (Cluthy (1980) FEMS Microbiol. Lett. 8:1-7).
- Gamma-exotoxins are various proteolytic enzymes, chitinases and proteases. The toxic effects of gamma exotoxins are only expressed in combination with beta-exotoxin or delta- endotoxin. Forsberg et al. (1976) "Bacillus thuringiensis: Its effects in Environmental Quality," National Research Council of Canada.
- Bacillus spp. includes B. subtilis, B. cereus, B. mycoides, B. thuringiensis strains that exhibit antifungal activity.
- Bacillus spp. includes B. subtilis, B. cereus, B. mycoides, B. thuringiensis strains that exhibit antifungal activity.
- Zwittermicin-A is a water soluble, acid stable linear aminopolyol molecule (see, He et al, (1994) Tetra Lett. 35(16):2499-2502.
- biopesticides include, for example, wettable powders, dry flowables, microencapsulation of effective agents, liquid or solid formulations of antibiotic fractions from suitable cultures.
- wettable powders dry flowables
- microencapsulation of effective agents liquid or solid formulations of antibiotic fractions from suitable cultures.
- Bacillus metabolites are the cyclic lipopeptides of the iturin class, some of which are potent fungicidal agents. These agents consist of a cyclic octapeptide with seven ⁇ -amino acids and one ⁇ -amino acid with an aliphatic side chain. There are several groups of iturins that differ in order and content of the amino acid sequence. These are shown in Table 1 below. Generally, a suite of related molecules is produced with differences in the length and branching of the aliphatic amino acid residue.
- R CH 3 .CH(CH 3 ) 2 , CH 3 CH 2 CH
- a research group at the USDA has investigated the structure/activity relationship of the iturins by synthesizing a number of analogs differing in the amino acid chain length.
- the researchers reported that the activity of the iturins increased with the length of the fatty acid side chain and the terminal branching in the order iso>normal>antiiso (Bland et al. (1995) Proc. Plant Growth Regulation Soc. Am. 22nd: 105-107). They also state that the "amounts of iturins obtained from natural production are inadequate to be commercially viable" based on their work with a number of iturin producing strains o ⁇ Bacillus.
- Another groups of cyclic lipopeptides isolated from B. cereus are the plipastatins.
- Surfactin contains a C14 or C15 ⁇ -hydroxy fatty acid linked by a lactone ring to a heptapeptide moiety with a LLDLLDL (SEQ ID NO:l) sequence (Arima et al. (1968) Biochem. Biophys. Res. Commun 31:488-494. Sandrin et al. ((1990) Biotechnol. Appl. Biochem. 12:370-375) found B. subtilis strains that produced both surfactin and iturin A, the bacillomycins F and L and mycosubtilin.
- the novel microorganism AQ713 discovered by the inventors, previously thought to be a strain o ⁇ Bacillus megaterium and now identified as a strain of Bacillus subtilis, produces A iturins, plipastatins and surfactins. Production of this combination of lipopeptides by a microorganism has not been previously reported.
- the inventors have discovered that AQ713 also produces a newly described group of compounds designated as "agrastatins.”
- the combination of all three of the above known compounds with the novel agrastatins is also novel.
- a novel antibiotic-producing and metabolite-producing strain o ⁇ Bacillus subtilis previously identified as Bacillus megaterium, is provided that exhibits broad fungicidal and bactericidal activity and also exhibits insecticidal activity. Also provided is a novel metabolite from the novel B. subtilis with activity against foliar and subterranean insects. Also provided is a method of treating or protecting plants from fungal, bacterial and insect infections comprising the step of applying an effective amount of the antibiotic-producing Bacillus subtilis.
- the antibiotic- producing Bacillus subtilis can be provided as a suspension in a whole broth culture or as an antibiotic-containing supernatant obtained from a whole broth culture of the antibiotic-producing strain o ⁇ Bacillus.
- Also provided is a method of treating or protecting plant roots from subterranean infestations comprising the step of applying an effective amount of the novel metabolite- producing Bacillus subtilis, whole broth culture or culture supernatant.
- the novel metabolite-producing Bacillus subtilis can be provided as a suspension in a whole broth culture or as a metabolite-containing supernatant or a purified metabolite obtained from a whole broth culture of the microorganism.
- novel compounds, agrastatins produced by the novel strain AQ713 and a novel combination of compounds comprising iturin A, a plipastatin, a surfactin and an agrastatin.
- Figure 1 shows the structure of the plipastatin antibiotics (SEQ ID NO:2).
- Figure 2 shows the HPLC chromatogram of AQ713 metabolites.
- the present invention provides a novel strain, AQ713, o ⁇ Bacillus subtilis, previously identified as a Bacillus megaterium, or mutants thereof with the broad antifungal, insecticidal and antibacterial activity.
- This novel strain is designated AQ713 and was deposited with the NRRL on March 7, 1997 under the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure under Accession No. B-21661. It was subsequently identified as Bacillus subtilis by the American Type Culture Collection (ATCC).
- ATCC American Type Culture Collection
- the invention also includes methods of treating plant roots or soil to control insect infestations with a bacterial suspension of AQ713 or a metabolite-containing supernatant of a culture of AQ713 or purified metabolites from strain AQ713.
- the invention also includes methods of preventing and treating fungal, bacterial and insect diseases in plants using such bacterial strains or antibiotic- containing supematants or pure antibiotics obtained from such bacterial strains.
- the invention also includes methods of treating plant foliage, roots or the soil surrounding the plant to control insects and insect larvae with a bacterial suspension of AQ713 or a metabolite-containing supernatant of a culture of AQ713 or purified metabolites from strain AQ713.
- the invention also includes a solvent-extractable metabolite with activity on insects with a molecular weight of less than 10,000
- the invention further includes novel compounds, agrastatins, produced by the novel microorganism. Also included is a novel combination comprising an A- type iturin, a plipastatin, a surfactin and an agrastatin.
- biological control is defined as control of a pathogen or insect by the use of a second organism.
- Known mechanisms of biological control include enteric bacteria that control root rot by out-competing fungi for space on the surface of the root.
- Bacterial toxins, such as antibiotics, have been used to control pathogens.
- the toxin can be isolated and applied directly to the plant or the bacterial species may administered so it produces the toxin in situ.
- bacteria includes any prokaryotic organism that does not have a distinct nucleus.
- fungus or “fungi” includes a wide variety of nucleated spore- bearing organisms that are devoid of chlorophyll. Examples of fungi include yeast, molds, mildews, rusts, and mushrooms.
- Fungicidal means the ability of a substance to increase mortality or inhibit the growth rate of fungi.
- a “mutant” is an organism bearing a mutant gene that expresses itself in the phenotype of the organism.
- Antibiotic includes any substance that is able to kill or inhibit a microorganism. Antibiotics may be produced by a microorganism or by a synthetic process or semisynthetic process. The term, therefore, includes a substance that inhibits or kills fungi, for example, zwittermicin-A or kanosamine.
- Antifungal includes any substance that is able to kill or inhibit the growth of fungi.
- the term “culturing” refers to the propagation of organisms on or in media of various kinds.
- Whole broth culture refers to a liquid culture containing both cells and media.
- Supernatant refers to the liquid broth remaining when cells grown in broth are removed by centrifugation, filtration, sedimentation, or other means well known in the art.
- an “effective amount” is an amount sufficient to effect beneficial or desired results.
- An effective amount can be administered in one or more administrations.
- an “effective amount” is that amount sufficient to ameliorate, stabilize, reverse, slow or delay progression of the fungal or bacterial disease states.
- insects includes all organisms in the class "Insecta.”
- Pre-adult insects refers to any form of an organism prior to the adult stage, including, for example, eggs, larvae, and nymphs.
- Insecticidal refers to the ability of a substance to increase mortality or inhibit growth rate of insects.
- Nematicidal refers to the ability of a substance to increase mortality or inhibit the growth rate of nematodes.
- Pesticidal refers to the ability of a substance to increase mortality or inhibit the growth rate of insects, nematodes and mites.
- “Positive control” means a compound known to have pesticidal activity.
- “Positive controls” include, but are not limited to commercially available chemical pesticides.
- the term “negative control” means a compound known not to have pesticidal activity. Examples of negative controls are water or ethyl acetate.
- the term “solvent” includes any liquid that holds another substance in solution.
- Solvent extractable refers to any compound that dissolves in a solvent and which then may be isolated from the solvent. Examples of solvents include, but are not limited to, organic solvents like ethyl acetate.
- metabolite refers to any compound, substance or byproduct of a fermentation of a microorganism that has pesticidal activity.
- Antibiotic as defined above is a metabolite specifically active against a microorganism.
- agrastatins refers to a group of novel compounds having the following structures (SEQ ID NO:3):
- R is a branched or straight aliphatic side chain, C8-C20; X is either Ala or Val; R, is an acetate or an ester derivative; and Glx is Gin or Glu.
- X is either Ala or Val; R, is an acetate or an ester derivative; and Glx is Gin or Glu.
- the present invention provides a method of treating or protecting plants from fungal, insect and bacterial infections comprising applying an effective amount of a supernatant obtained from a whole broth culture o ⁇ Bacillus subtilis AQ713 within the present invention.
- the supernatant may be obtained well known in the art including centrifugation, filtration, sedimentation and the like.
- the invention encompasses a method of treating or protecting plants from fungal, insect and bacterial infections comprising applying an effective amount of the whole broth of the novel strain Bacillus subtilis.
- the invention encompasses a method of treating or protecting plants from fungal, insect, and bacterial diseases comprising applying an effective amount of the antibiotic produced by the novel strain o ⁇ Bacillus subtilis.
- the present invention provides a method of treating or protecting plants and plant roots from insect and larvae infestations comprising applying an effective amount of a supernatant obtained from a whole broth culture of Bacillus subtilis AQ713 within the present invention. The supernatant may be obtained well known in the art including centrifugation, filtration, sedimentation and the like.
- the invention encompasses a method of treating or protecting plants and plant roots from insect and larvae infestations comprising applying an effective amount of the whole broth of the novel strain Bacillus subtilis.
- the invention encompasses a method of treating or protecting plant roots from insect infestations comprising applying an effective amount of the metabolite produced by the novel strain o ⁇ Bacillus subtilis.
- compositions within the present invention can be formulated as wettable powders, granules and the like, or can be microencapsulated in a suitable medium and the like.
- suitable formulations include, but are not limited to soluble powders, wettable granules, dry flowables, aqueous flowables, wettable dispersible granules, emulsifiable concentrates and aqueous suspensions.
- suitable formulations will be known to those skilled in the art.
- a novel group of compounds designated "agrastatins” are provided. These compounds exhibit antibacterial and antifungal activity in addition to anti-insect activity.
- a novel combination comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin is provided.
- methods of treating or protecting plants from insect, fungal and bacterial diseases comprising applying an effective amount of a novel combination of compounds comprising an A-type iturin, a plipastatin, a surfactin and an agrastatin are provided.
- a lipopeptide extract isolated from strain AQ713 with insecticidal activity and a surfactin lipopeptide isolated from strain AQ713 with insecticidal activity.
- this invention also provides a method for treating or protecting plants and/or fruits from insect infestations by applying an effective amount of the isolated lipofectin or isolated surfactin to the foliage, roots or the soil surrounding the plants or roots.
- These isolated compositions can be combined with other known pesticides or insecticides and may be formulated as described above for AQ713 and applied as wettable powders, granules, flowables or microencapsulated.
- Biolog, Inc. Hayward, CA
- Bochner (1989) Nature 339:157-158 The Biolog microplate is comprised of prefilled and dried panel wells with 95 different carbon substrate plates available for gram positive and gram negative bacteria.
- the isolate was grown in liquid medium at 28°C and after 24 hr a washed cell suspension (0.85% saline) was inoculated into each panel well of a GP Microplate (Biolog, Inc.). After 24 hr at 28°C, carbon utilization reactions were assessed. Substrate utilization profiles were then compared to the Biolog Gram- Positive Data Base (release 3.50) and isolated to closest similar species. Biolog results gave a similarity index of 0.883 to Bacillus megaterium.
- a more extensive characterization of AQ713 was conducted by the American Type Culture Collection, (ATCC) 10801 University Boulevard., Manassas, VA, 20110- 2209.
- Isolate identified as: Using the available physiological and biochemical data, this strain most closely resembles Bacillus subtilis.
- the motile cells are found in singly, with one endospore formed in the central or subterminal region. The cells are uniformly stained Gram positive.
- Bacillus samples were grown in a Bacillus culture media.
- Medium 2 contained 5% peptone, 5% dextrose, 3% yeast extract, 3% malt extract, 1.5% proflo cotton seed extract (59% protein, 4.26% fat, 6.73% ash, 3.19% fiber and trace amounts of gossypol; the balance is water), 10% soy flour, and 0.5% MgSO 4 x 7H 2 O.
- Medium 3 contained the same ingredients, except with 20% peptone and 3.4% KH 2 PO 4 and 4.3% K 2 HPO 4 .
- One day old streaked cultures were used to inoculate 250 mL baffled shake flasks. Flasks were shaken at 200 ⁇ m at 29°C for 5 days.
- To assay insecticidal activity 35 mL of culture broth were centrifuged at 5,200 ⁇ m for 20 minutes and the supernatant used in microassay described below.
- Assays were performed in 96-well microplates. Each well contained a solid agar substrate, a test organism and either a positive control, a negative control or supernatant obtained as described in Example 1 from the novel Bacillus strain.
- a 1 ppm solution of Avid® (avermectin) was used as a positive control. Deionized water was used as a negative control. Two replicates of test sample or control were used for each assay. 40 ⁇ L of supernatant sample or whole broth grown in medium 1, 2 or 3 were dispensed into each sample well. Plates were then placed in a fume hood to dry for approximately 2-3 hours until the agar solution was dried.
- Test organisms were either pre-adult corn rootworm (Diabrotica undecimpunctata), pre-adult German cockroaches (Blatella germanica), pre-adult beet armyworms (Spodoptera exigua), pre-adult flies (Drosophila melanogaster), or the N2 strain of the nematode Caenorhabditis elegans. Test organisms were diluted in 0.1% agar to a concentration of approximately 5 organisms per 25 ⁇ L of agar dispensed into each well. The microplate was sealed with an airtight substance such as Mylar®, and each well ventilated with a pin press. The plates were incubated at 27°C for up to 7 days.
- Table 3 A Score Rating of AQ713 against Insect Pests Supernatant Test 1
- AQ713 was tested again against beet armyworm and corn rootworm in a third medium in shake flask or a 10 liter fermenter, called medium 4. It contained the same ingredients as medium 3 without proflo cotton seed extract. All other procedures were the same as above. The test was repeated two or three times.
- Table 3B Score Rating of AQ713 against Insect Pests Supernatant Test
- AQ713 was tested in medium 4 using batches of AQ713 grown twice in two different 10 liter fermenters and twice in one 400 liter fermenters for 48 hours.
- the green peach aphid, Myzus persicae was tested in addition to corn rootworm and armyworm. Both whole broth (WB) and supernatant (S) were tested from one of the 400 liter batches.
- WB whole broth
- S supernatant
- the bottom of the well was covered with aphids.
- a column of wells When a column of wells is filled, the plate is capped with a cap strip to keep the aphids in place.
- the test plates are incubated at 20-22°C. The test is rated in 48 hours, using a microscope to count the number of aphids alive and dead. The wells are then given a score on a scale from 1 to 4 as with the other insects (4 is no dead insects, 1 is 100% kill).
- AQ713 produces a solvent-extractable metabolite that kills com rootworm.
- a 50-mL culture of AQ713 was grown in media 2. One mL was placed into a micro fuge tube and spun at 12,000 ⁇ m for 15 minutes. The supernatant was removed. 500 microliters of supernatant was placed on top of a 10,000 Dalton molecular weight centricon filter. These were centrifuged according to the manufacturer's instructions (12,000 ⁇ m for 35 minutes). The filtrate was collected and the retentate recovered by centrifugation and washing of the filter.
- the dried organic extract was redissolved in 2.5 mL acetone. A 40 ⁇ L aliquot was removed and diluted to 800 ⁇ L with 70% acetone/water. This is a 1 OX concentration of the organic extract.
- a 96-well plate assay (described below) plant pathogen assay with Pythium ultimum and Botrytis cinerea was conducted to determine activity of the organic extract. The whole broth gave 100% control (score of 1), but the 10X organic extract gave no control of the two plant pathogens (score of 4). This indicates that the active antibiotics, unlike the com rootworm active metabolites produced by AQ713 are not extractable in an organic solvent such as ethyl acetate.
- a butanol extract was made of the fermentation broth by first extracting the broth two times with an equal volume of ethyl acetate and separating the layers. The aqueous fraction was then extracted two times with an equal volume of butanol. The butanol extracts were combined and solvent was removed with a rotary evaporator. A powder was obtained by freeze drying the resulting extract.
- the powder was dissolved in 80% acetonitrile/water and sonicated.
- the solution was applied to a C-18 solid phase extraction (SPE) cartridge that had been activated with methanol and equilibrated with 80% acetonitrile/water.
- SPE solid phase extraction
- the SPE cartridge was eluted with 80% ACN/water and this eluent was collected and the solvents removed. The eluent was further purified by HPLC.
- a C-18 HPLC column (1 cm X 25 cm) was used (UN detection at 210 nm) with an acetonitrile + 0.05% TF A/water + 0.05% TFA solvent gradient as follows: 0-20 minutes, 33% AC ⁇ ; 20- 30 minutes, 40% AC ⁇ ; 30-45 minutes, 45-55% AC ⁇ ; and 45-63 minutes, 55% AC ⁇ .
- An HPLC chromatogram of AQ713 shows the presence of the iturins, iturin- like compounds (plipastatins and agrastatins) and surfactins, see Figure 1. Iturins A2, A3, A4, A7 and A6 were identified by a combination of ⁇ MR data and LC mass spectrometry data and comparison to literature values.
- agrastatin A was found to contain the following amino acids: Thr; 3 Glu; Pro; Ala; Val; 2 Tyr; and Om. This make up differs from plipastatin A by the presence of Val and the loss of He.
- the molecular weight of agrastatin A was determined to be 1448 which corresponds to the following structure (SEQ ID ⁇ O:4):
- spores were scraped from the surface of petri plates and 0.1-0.2 mL aliquots of deionized water and spore suspension (concentration approximately 2 X 10 6 spores/mL) of pathogen were spread onto the agar.
- AQ713 was grown for 72 hours in the medium 2 or 3 as described in Example 2. To obtain supematants, the whole broth culture was centrifuged at 5,200 ⁇ m for 20 minutes. The fungal plant pathogens were pipetted onto the 96-well plates (8 wells/pathogen). The presence or absence of fungal growth was recorded for each of 8 wells. Approximately 40 ⁇ L of AQ713 supernatant or 20 ⁇ L of whole broth was added to each well. A score of "1" means complete inhibition of fungal growth. A score of "4" means no inhibition of fungal growth. Results are shown in Table 7.
- Botrytis cinerea 1 Botrytis cinerea 1
- Example 9 Activity of AQ713 Against Bacterial Plant Pathogens
- a standard agar diffusion assay was set up as in Example 6. A lawn of each bacterial pathogen was spread over the surface of a petri plate. 100 ⁇ L of AQ713 whole broth grown in medium 2 was placed in each well. The size of the zone was measured in millimeters.
- AQ713 was active against all species of bacterial plant pathogens tested in vitro.
- AQ713 The activity of AQ713 was tested against gray mold, Botrytis cinerea, on beans and geranium leaves, Alternaria solani on tomato seedlings, and downy mildew of lettuce, Bremia lactucae.
- a second test was set up using detached tomato seedlings (stems broken off at the ground level) placed in mason jars filled with water put under domes and stored as above. The plants were sprayed as above and the symptoms of A. solani were recorded four days later. There were no symptoms on the negative control. On the positive control, there were uniform lesions over the seedlings. The AQ713 treatment was rated 1 (few or no lesions). Two days later, the plants in the positive control were destroyed, but the AQ713 treated seedlings were virtually clean and looked the same as the negative controls (water sprayed plants).
- lettuce seeds were planted in a layer of sterilized potting mix containing peat, perlite and vermiculite in small clear plastic plant condominiums about 8 centimeters high and wide. After the lettuce germinated (one week), the lettuce seedlings were sprayed with the AQ713 broth or supernatant sample. The plants were allowed to dry and then downy mildew spore suspension from infected lettuce seedlings was sprayed onto the seedlings. The plastic covers were placed over the plants and incubated at 18-20°C in a Percival incubator. One week later, the test was evaluated. AQ713 did not prevent downy mildew from Bremia on lettuce seedlings.
- Grape Downy Mildew AQ713 was grown in a soy-based medium in a 400 liter fermenter for 48 hours. Grape plants (cultivar Chardonnay) were sprayed with a hand-held sprayer to run-off with whole broth from the 400 liter fermentation ran diluted with sterile water to 0.5X and 0.25X concentrations. When the foliage dried, the plants were sprayed a second time. After drying, the plants were inoculated with the pathogen causing grape downy mildew, Plasmopara viticola. Three plants were treated for each dose. Each plant was evaluated by estimating the percent disease control based on a scale from 0 to 100% control. 100% control is a plant with no visible lesions. A chemical fungicide, metalaxyl, was used for comparison. The results were as follows:
- AQ713 was grown in a soy-based medium in a 400 liter fermenter for 48 hours. Squash plants (Crookneck and Aco ) were sprayed with a hand-held sprayer to mn-off with whole broth from the 400 liter fermentation ran and a sample diluted with sterile water to 0.5 X concentration. After drying, the plants were inoculated with the squash powdery mildew pathogen, Sphaerothecafuliginea. Two plants were treated for each dose. Spray dried powder of the whole broth was also tested. The 400 liter fermentation broth was spray dried to remove the water. 10% and 2.5% spray dried powder solutions were sprayed on the plants to run-off as above. The incidence of powdery mildew disease was rated on a score from 0 to 5. The 5 rating is 100% disease whereas the 0 rating is no disease. The results are shown below in Table 10.
- AQ713 was grown in a soy-based medium for 72 hours in a 250 mL shake flask.
- the disease, causative pathogen and host are listed in Table 11 below. This whole broth culture was tested on the plants as shown in Table 11 below.
- Each broth was sprayed to run-off at IX concentration on the test plants with a hand held sprayer, allowed to dry and then sprayed a second time.
- Three plants were treated for each disease and treatment. After drying, the plants were inoculated with the pathogens. Each plant was evaluated by estimating the percent disease control based on a scale from 0 to 100% control. 100% control refers to a plant with no visible lesions. Chemical fungicides were used for comparison Disease index is the severity of the disease on the untreated control. Table 12
- AQ713 showed activity that was equivalent to chemical fungicides on all the pathogens tested.
- Bacillus strain AQ713 was grown in a ten liter fermenter in a soy-based medium for 48 hours. The whole broth culture at IX strength was sprayed onto three week-old cauliflower and Brassels sprouts plants at the full cotyledon stage with an artist's air brash powered by compressed air. Three replicates of 15-25 seedlings/pot were sprayed per treatment. QuadrisTM, an azoxystrobin fungicide from Zeneca, was also sprayed on plants (three per treatment) at rates of 250 ppm and 125 ppm.
- a spore suspension of downy mildew, Peronospora parasitica, at 1-5 X 10 4 spores/mL was sprayed onto the Brassica plants after the AQ713 and Quadris sprays dried. The plants were held at 15-17°C for 24 hours for infection, then the seedlings were incubated at 20-24°C for six days. The pots were returned to 15-17°C overnight to allow sporulation of the pathogen until the test was rated. Each plant was evaluated by estimating the percent disease control based on a scale from 0 to 100% control. 100% control is a plant with no sporalating lesions. The results averaged across replicate pots are shown below in Table 14.
- AQ713 was grown in a ten liter fermenter in a soy-based medium for 72 hours.
- the bacterial culture was diluted with sterile water to 0.5X and 0.25X concentrations.
- the culture at IX, 0.5X and 0.25X concentrations was sprayed onto three week-old pepper plants with an artist's air brush powered by compressed air. Three plants were sprayed per treatment.
- QuadrisTM an azoxystrobin fungicide from Zeneca, was also sprayed on plants (three per treatment) at concentrations of 500 ppm, 250 ppm and 125 ppm.
- combinations of Quadris plus the whole broth culture of AQ713 in a 1 :1 ratio were sprayed onto pepper plants (three per treatment).
- the whole broth is vortexed and the pH is adjusted to 1.5 with HC1, vortexed again, then centrifuged for 15 minutes at 10,500 ⁇ m.
- the supernatant is poured off and discarded.
- the pellet is suspended in 80% acetonitrile/water (ACN/H 2 0) and then sonicated for 30 minutes.
- the sample is centrifuged again for 15 minutes and then the pellet is suspended again in 80% ACN/H 2 0 by vortexing. It is centrifuged again for 15 minutes and then dried in a speed vacuum overnight. The sample is then redissolved in 80% ACN/H 2 0.
- Surfactin one of the lipopeptides, was tested alone. Surfactin was purchased from Sigma Chemicals (St. Louis, MO) and is identical to the surfactin in AQ713 as verified by HPLC. Therefore, Sigma surfactin was used in tests against insects.
- the lipopeptide extract of strain AQ713 is insecticidal. Surfactin alone shows activity against aphids and com rootworm, but not armyworm. Therefore, the insecticidal activity of strain AQ713 can be partially explained by the lipopeptides in strain AQ713.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR9915339-4A BR9915339A (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
IL14303799A IL143037A0 (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
JP2000582413A JP2002530290A (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
CA002350782A CA2350782A1 (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
SK656-2001A SK6562001A3 (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
HU0104242A HUP0104242A2 (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
AU12233/00A AU1223300A (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
EP99972218A EP1131342A4 (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
KR1020017006053A KR20010075707A (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
NO20012344A NO20012344L (en) | 1998-11-12 | 2001-05-11 | Compositions and Methods for Controlling Plant Diseases |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10826698P | 1998-11-12 | 1998-11-12 | |
US60/108,266 | 1998-11-12 | ||
US09/223,587 | 1998-12-30 | ||
US09/223,587 US6103228A (en) | 1997-05-09 | 1998-12-30 | Compositions and methods for controlling plant pests |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000029426A1 true WO2000029426A1 (en) | 2000-05-25 |
Family
ID=26805719
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1999/024827 WO2000029426A1 (en) | 1998-11-12 | 1999-10-21 | Compositions and methods for controlling plant pests |
Country Status (16)
Country | Link |
---|---|
US (3) | US6103228A (en) |
EP (1) | EP1131342A4 (en) |
JP (1) | JP2002530290A (en) |
KR (1) | KR20010075707A (en) |
CN (1) | CN1335854A (en) |
AU (1) | AU1223300A (en) |
BR (1) | BR9915339A (en) |
CA (1) | CA2350782A1 (en) |
CZ (1) | CZ20011620A3 (en) |
HU (1) | HUP0104242A2 (en) |
IL (1) | IL143037A0 (en) |
MX (1) | MXPA01004814A (en) |
NO (1) | NO20012344L (en) |
PL (1) | PL348637A1 (en) |
SK (1) | SK6562001A3 (en) |
WO (1) | WO2000029426A1 (en) |
Cited By (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004002510A1 (en) * | 2002-06-28 | 2004-01-08 | Faculte Universitaire Des Sciences Agronomiques De Gembloux | Novel use of lipopeptide preparations |
WO2009037242A2 (en) * | 2007-09-20 | 2009-03-26 | Basf Se | Combinations comprising a fungicidal strain and an active compound |
WO2010033714A1 (en) | 2008-09-17 | 2010-03-25 | Agraquest, Inc. | Method for using a bacillus subtilis strain to enhance animal health |
US7994138B2 (en) | 2004-06-01 | 2011-08-09 | Agscitech Inc. | Microbial biosurfactants as agents for controlling pests |
US8211828B2 (en) | 2007-01-19 | 2012-07-03 | Basf Se | Fungicidal mixtures of 1-methylpyrazol-4-ylcarboxanilides and azolopyrimidinylamines |
WO2012130221A2 (en) | 2011-04-01 | 2012-10-04 | Abitep Gmbh | Means for treating microbial diseases in cultivated plants |
WO2013060910A1 (en) * | 2011-10-28 | 2013-05-02 | Investigaciones Y Aplicaciones Biotecnologicas, S.L. | New strain of bacillus subtilis for combating plant diseases |
WO2013067275A1 (en) * | 2011-11-04 | 2013-05-10 | Agraquest. Inc. | Biocontrol of nematodes |
WO2013126387A2 (en) * | 2012-02-21 | 2013-08-29 | Dupont Nutrition Biosciences Aps | Composition |
WO2013178650A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the respiratory chain at complex iii |
WO2014147534A1 (en) * | 2013-03-20 | 2014-09-25 | Basf Corporation | Synergistic compositions comprising a bacillus subtilis strain and a pesticide |
WO2014178032A1 (en) | 2013-05-03 | 2014-11-06 | Universidad Eafit | Production process for biomass and fengycin metabolites of bacillus species and compositions thereof for biological pest control |
EP2871245A1 (en) * | 2013-11-08 | 2015-05-13 | Symborg, S.L. | A bacillus subtilis strain deposited under deposit number CECT 8258 and method for protecting or treating plants |
AU2014203689B2 (en) * | 2007-09-20 | 2016-02-04 | Bayer Cropscience Lp | Combinations comprising a fungicidal strain and an active compound |
US9364006B2 (en) | 2012-05-30 | 2016-06-14 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
US9380787B2 (en) | 2012-05-30 | 2016-07-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of ATP production and inhibitors of the cell wall synthesis |
US9386773B2 (en) | 2012-05-30 | 2016-07-12 | Bayer Cropscience Ag | Compositions comprising a biological control agent and a fungicide from the group consisting of inhibitors of the respiratory chain at complex I or II |
US9457054B2 (en) | 2010-03-17 | 2016-10-04 | Bayer Cropscience Lp | Method for using a Bacillus subtilis strain for prophylaxis and treatment of gastro-intestinal conditions |
US9596860B2 (en) | 2012-05-30 | 2017-03-21 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the mitosis and cell division or compounds having a multi-site action |
US9700056B2 (en) | 2012-05-30 | 2017-07-11 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis |
EP3205208A1 (en) | 2016-02-09 | 2017-08-16 | Basf Se | Mixtures and compositions comprising paenibacillus strains or fusaricidins and chemical pesticides |
EP3205209A1 (en) | 2016-02-09 | 2017-08-16 | Basf Se | Mixtures and compositions comprising paenibacillus strains or metabolites thereof and other biopesticides |
US9795138B2 (en) | 2009-05-06 | 2017-10-24 | Bayer Cropscience Lp | Method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure |
US10076119B2 (en) | 2012-11-22 | 2018-09-18 | Basf Corporation | Pesticidal mixtures |
US10251400B2 (en) | 2014-05-23 | 2019-04-09 | Basf Se | Mixtures comprising a Bacillus strain and a pesticide |
WO2020084609A1 (en) * | 2018-10-21 | 2020-04-30 | Grace Breeding Ltd. | Dual-route administration of composition for improved protection of plants against pathogens |
WO2020092381A1 (en) * | 2018-10-30 | 2020-05-07 | AgBiome, Inc. | Bacterial compositions and methods for controlling plant pests and improving plant health |
US10779536B2 (en) | 2014-11-07 | 2020-09-22 | Basf Se | Pesticidal mixtures |
US10905122B2 (en) | 2016-03-16 | 2021-02-02 | Basf Se | Use of tetrazolinones for combating resistant phytopathogenic fungi on cereals |
US10986842B2 (en) | 2015-11-10 | 2021-04-27 | Chr. Hansen A/S | Microbial pesticidal composition and production thereof |
US11241012B2 (en) | 2016-03-16 | 2022-02-08 | Basf Se | Use of tetrazolinones for combating resistant phytopathogenic fungi on soybean |
US11425909B2 (en) | 2016-03-16 | 2022-08-30 | Basf Se | Use of tetrazolinones for combating resistant phytopathogenic fungi on fruits |
Families Citing this family (72)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
HUP0200562A2 (en) * | 1999-03-30 | 2002-06-29 | Agraquest Inc | A strain of baccilus pumilus for controlling plant diseases |
US6245551B1 (en) | 1999-03-30 | 2001-06-12 | Agraquest, Inc. | Strain of Bacillus pumilus for controlling plant diseases caused by fungi |
US6808917B1 (en) | 2001-02-02 | 2004-10-26 | Thomas D. Johnson | Controlling plant pathogens with fungal/bacterial anatagonist combinations |
WO2003013251A1 (en) * | 2001-08-10 | 2003-02-20 | Showa Denko K.K. | Fungicidal and/or bactericidal composition, production process thereof and sterilization method using the composition |
JP4071036B2 (en) * | 2001-11-26 | 2008-04-02 | クミアイ化学工業株式会社 | Bacillus sp. D747 strain and plant disease control agent and pest control agent using the same |
US6589524B1 (en) | 2002-02-07 | 2003-07-08 | Ecomicrobials, Llc | Strains of Bacillus for biological control of pathogenic fungi |
JP4313980B2 (en) * | 2002-04-10 | 2009-08-12 | 社団法人農林水産先端技術産業振興センター | A method for controlling diseases and pests of gramineous plants using symbiotic fungi, seeds combined with control agents and control agents |
JP4189224B2 (en) * | 2003-01-14 | 2008-12-03 | 株式会社カンサイ | Microorganisms belonging to the genus Bacillus and control agents using the same |
US7244607B2 (en) * | 2003-10-01 | 2007-07-17 | The United States Of America, As Represented By The Secretary Of Agriculture | Chromobacterium subtsugae sp. nov. for control of insect pests |
CN1311067C (en) * | 2004-04-14 | 2007-04-18 | 北京天地绿园农业科学研究院 | Fermentation and preparation for composite bacterial prepn and organic bacterial manure for preventing and controlling soil plant diseases and insect pests |
US20060033091A1 (en) * | 2004-07-23 | 2006-02-16 | Lmt Mercer Group Inc. | Thermoplastic fencing construction and method of assembly thereof |
WO2008131002A2 (en) | 2007-04-16 | 2008-10-30 | Modular Genetics, Inc. | Generation of acyl amino acids |
CL2008000602A1 (en) * | 2008-02-27 | 2008-06-27 | Univ Austral De Chile | BACILLUS SUBTILIS CEPA WITH DEPOSIT PTA-8805 WITH ANTAGONIST ACTIVITY ON RHIZOCTONIA SOLANI; FORMULATION OF CONCENTRATED BIOPRODUCTS AND CONCENTRATED LIQUID THAT INCLUDES SUCH BACTERIA; USE OF BIOPRODUCTS TO REDUCE AND CONTROL BIOLOGICAL |
EP2179652B1 (en) | 2008-09-10 | 2019-05-08 | ABiTEP GmbH Gesellschaft für AgroBioTechnische Entwicklung und Produktion | Use of an antibacterial agent for treating bacterial infection in crop plants |
PE20120536A1 (en) * | 2009-03-26 | 2012-05-05 | Basf Se | COMBINATION OF A SYNTHETIC FUNGICIDE AND BACILLUS SUBTILIS QST 173 AGENT FOR THE CONTROL OF PHYTOPATOGENIC FUNGI |
WO2011099878A2 (en) | 2010-02-12 | 2011-08-18 | Grecilda Sanchez Zaballero | Organic pesticide |
US8951585B2 (en) | 2010-02-25 | 2015-02-10 | Marrone Bio Innovations, Inc. | Compositions and methods for modulating plant parasitic nematodes |
CN101892176B (en) * | 2010-03-23 | 2012-07-04 | 天津科技大学 | Microbe generating antifungal lipopeptid and preparation method and application of antifungal lipopeptid thereof |
CN101870959B (en) * | 2010-06-11 | 2012-06-06 | 河北省科学院生物研究所 | Application of bsacillus subtilis, microbial inoculum thereof and preparations thereof in fruit fresh keeping field |
ES2607059T3 (en) | 2010-09-20 | 2017-03-29 | Wisconsin Alumni Research Foundation | Xenorhabdus, lipopeptide and mosquitocidal procedures |
TW201225844A (en) | 2010-10-25 | 2012-07-01 | Marrone Bio Innovations Inc | Chromobacterium bioactive compositions and metabolites |
ES2649937T3 (en) | 2010-12-21 | 2018-01-16 | Bayer Cropscience Lp | Bacillus sandpaper type mutants and their use procedures to improve plant growth, promote plant health and control diseases and pests |
RU2013157207A (en) | 2011-05-24 | 2015-06-27 | БАЙЕР КРОПСАЙЕНС ЭлПи | SYNERGETIC COMBINATIONS OF POLYENE FUNGICIDES AND NERIBOSOMAL PEPTIDES AND RELATED APPLICATION METHODS |
AU2012259893B2 (en) * | 2011-05-26 | 2015-09-24 | Sds Biotech K.K. | Strain belonging to Bacillus genus, microbiological agent, and plant cultivation method |
PT2736340T (en) | 2011-07-25 | 2018-03-22 | Bayer Cropscience Lp | Biocontrol of nematodes |
ES2613806T3 (en) | 2011-07-26 | 2017-05-26 | The Medicines Company | Diffusion test with oritavancin disc |
FR2980802B1 (en) | 2011-10-03 | 2014-12-26 | Univ Lille 1 Sciences Et Technologies Ustl | METHOD FOR PRODUCING BIOSURFACTANTS AND DEVICE FOR IMPLEMENTING THE SAME |
CA2852531C (en) | 2011-10-25 | 2020-04-28 | Marrone Bio Innovations, Inc. | Chromobacterium formulations, compostions, metabolites and their uses |
WO2013152353A1 (en) | 2012-04-06 | 2013-10-10 | Bayer Cropscience Lp | Biocontrol of nematodes |
US9017442B2 (en) | 2012-04-20 | 2015-04-28 | Novozymes Bioag A/S | Use of synergistic microorganisms and nutrients to produce signals that facilitate the germination and plant root colonization of mycorrhizal fungi in phosphorus rich environments |
JP6285423B2 (en) * | 2012-05-30 | 2018-02-28 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | Composition comprising a biopesticide and an insecticide |
BR112014029118A2 (en) * | 2012-05-30 | 2017-06-27 | Bayer Cropscience Ag | compositions comprising a biological control agent and an insecticide |
CN104837343A (en) | 2012-11-29 | 2015-08-12 | 拜尔农作物科学有限合伙人公司 | Methods of controlling fungal pathogens using polyene fungicides |
EP2925137A1 (en) * | 2012-11-30 | 2015-10-07 | Bayer CropScience AG | Binary fungicidal or pesticidal mixture |
US8808719B1 (en) | 2013-03-15 | 2014-08-19 | Marrone Bio Innovations, Inc. | Use of Chromobacterium substugae formulations, compostions and compounds to modulate cornworm rootworm larvae infestation |
WO2014151837A1 (en) | 2013-03-15 | 2014-09-25 | Bayer Cropscience Lp | A bacillus subtilis strain for use in maintaining or improving the paw health of poultry |
CN106535642A (en) * | 2014-02-10 | 2017-03-22 | 爱贝克斯生态学有限责任公司 | Bio-derived compositions for use in agriculture |
BR112016027807B1 (en) | 2014-05-28 | 2021-09-28 | Bayer Cropscience Lp. | FUNGICIDE COMPOSITIONS AND THEIR METHOD OF PRODUCTION, AS WELL AS METHODS FOR CONTROL AND TREATMENT OF FUNGIAL AND BACTERIAL DISEASES IN PLANTS, PLANT OR PART OF PLANT AND SEED |
AP2017009761A0 (en) * | 2014-08-16 | 2017-02-28 | Dcm Shriram Ltd | Novel bacterium of bacillus genus and uses thereof |
CN107105674B (en) | 2014-12-22 | 2020-10-02 | 拜耳作物科学有限合伙公司 | Methods of treating or preventing pineapple disease using bacillus subtilis or bacillus pumilus strains |
CN107960103B (en) | 2014-12-29 | 2021-12-03 | Fmc有限公司 | Microbial compositions and methods of use for promoting plant growth and treating plant diseases |
BR112017014057A2 (en) * | 2014-12-29 | 2018-01-16 | Fmc Corp | bacillus amiloliquefaciens rti472 compositions and methods of use to benefit plant growth and treat plant diseases |
US11371066B2 (en) | 2015-07-13 | 2022-06-28 | Modular Genetics, Inc. | Generation of acyl alcohols |
CN109068649A (en) | 2016-04-24 | 2018-12-21 | 拜耳农作物科学股份公司 | Fluopyram and/or bacillus subtilis are used to prevent and treat the purposes of the wilt disease of Musaceae plant |
MX2017012136A (en) * | 2016-05-22 | 2019-05-30 | Ibex Bionomics Llc | Enhancer compositions for agricultural chemicals and agricultural chemical compositions. |
EP3400801A1 (en) | 2017-05-10 | 2018-11-14 | Bayer CropScience Aktiengesellschaft | Plant health effect of purpureocillium lilacinum |
FR3061410B1 (en) * | 2016-12-30 | 2020-07-10 | Lipofabrik | BIOSTIMULANT COMPOSITION OF PLANT GROWTH OBTAINED FROM SURNANTANT OF BACILLUS STRAIN CULTURE SP. |
WO2018140542A1 (en) | 2017-01-26 | 2018-08-02 | Bayer Cropscience Lp | Method of promoting bacillus spore germination |
US20200022375A1 (en) * | 2017-03-30 | 2020-01-23 | Advanced Biological Marketing, Inc, | Alleviation of corn rootworm damage with microbial seed treatments |
US11795197B2 (en) | 2017-06-07 | 2023-10-24 | Cedars-Sinai Medical Center | Peptide compositions for immuno-oncology molecular imaging and targeted drug delivery |
CN108271776B (en) * | 2017-12-25 | 2021-06-11 | 武汉科诺生物科技股份有限公司 | Use of bacillus lipopeptide metabolite as microbial agent aid |
WO2019165413A1 (en) | 2018-02-26 | 2019-08-29 | Locus Agriculture Ip Company, Llc | Materials and methods for control of insect pests using entomopathogenic fungi |
US11447430B2 (en) | 2018-05-08 | 2022-09-20 | Locus Agriculture Ip Company, Llc | Microbe-based products for enhancing plant root and immune health |
CN109161506A (en) * | 2018-09-20 | 2019-01-08 | 宁国市百立德生物科技有限公司 | One bacillus subtilis and its application |
US20210360930A1 (en) * | 2018-09-27 | 2021-11-25 | Locus Ip Company, Llc | Compositions and Methods for Controlling Pathogens in Livestock Production Operations |
US11807862B2 (en) | 2019-01-15 | 2023-11-07 | Seminis Vegetable Seeds, Inc. | Green bean plants with improved disease resistance |
WO2020200959A1 (en) | 2019-03-29 | 2020-10-08 | Bayer Aktiengesellschaft | Method for controlling ganoderma disease in oil palm |
WO2020210074A1 (en) | 2019-04-12 | 2020-10-15 | Locus Ip Company, Llc | Pasture treatments for enhanced carbon sequestration and reduction in livestock-produced greenhouse gas emissions |
AU2020288624A1 (en) | 2019-06-04 | 2022-02-03 | Cocoon Biotech Inc. | Silk-based products, formulations, and methods of use |
WO2021022069A1 (en) | 2019-08-01 | 2021-02-04 | Bayer Cropscience Lp | Method of improving cold stress tolerance and crop safety |
AU2021204717A1 (en) | 2020-07-15 | 2022-02-03 | Seminis Vegetable Seeds, Inc. | Green Bean Plants with Improved Disease Resistance |
AU2021328578A1 (en) | 2020-08-18 | 2023-03-16 | Bayer Cropscience Lp | Compositions and methods for controlling fungal diseases in plants |
CN114703078B (en) * | 2020-12-16 | 2023-08-29 | 沈阳恩柽研究院有限公司 | Indolyl golden fungus and its application |
EP4263799A1 (en) | 2020-12-17 | 2023-10-25 | Basf Se | Spore compositions, production and uses thereof |
CN113142213B (en) * | 2021-04-25 | 2022-02-18 | 河北省科学院生物研究所 | Application of surfactin family lipopeptide in pest control |
CN113100237B (en) | 2021-04-25 | 2021-11-12 | 河北省科学院生物研究所 | Application of lipopeptide of plumogen family in pest control |
CN113115777B (en) * | 2021-04-25 | 2022-02-11 | 河北省科学院生物研究所 | Application of iturin family lipopeptide in pest control |
WO2022241209A1 (en) * | 2021-05-13 | 2022-11-17 | Bayer Cropscience Lp | Compositions and methods for use in identifying or quantifying microorganisms |
WO2023036938A1 (en) | 2021-09-10 | 2023-03-16 | Basf Se | Exopolysaccharide production microorganisms and uses thereof |
WO2023144351A1 (en) | 2022-01-28 | 2023-08-03 | Acies Bio D.O.O. | Control of plant pests by microbial agents |
CN115428808B (en) * | 2022-09-22 | 2023-03-28 | 西昌学院 | Potato late blight control medicament and application thereof |
WO2024088886A1 (en) | 2022-10-26 | 2024-05-02 | Bayer Aktiengesellschaft | Use for delaying greening and/or formation of solanine in potatoes |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR7981M (en) * | 1967-10-21 | 1970-06-08 | ||
US5047239A (en) * | 1984-05-01 | 1991-09-10 | The United States Of America As Represented By The Secretary Of Agriculture | Biological control of fruit rot |
JPS6151504A (en) * | 1984-08-21 | 1986-03-14 | Yasushi Ishii | Twist angle meter |
US4743689A (en) * | 1984-11-20 | 1988-05-10 | Nippon Kayaku Kabushiki Kaisha | Antibiotic derivative of adenine |
JPS62210996A (en) | 1986-03-11 | 1987-09-17 | Nippon Kayaku Co Ltd | Production of antibiotic substance emimycin |
US5049379A (en) * | 1987-07-27 | 1991-09-17 | Wisconsin Alumni Research Foundation | Fungicidal toxin and method and inoculum for controlling root rot and damping off |
GB8701234D0 (en) * | 1987-01-21 | 1987-02-25 | Agricultural Genetics Co | Strain of microorganism |
US4999192A (en) * | 1988-02-12 | 1991-03-12 | Mycogen Corporation | Novel coleopteran-active bacillus thuringiensis isolate |
GB8805394D0 (en) * | 1988-03-07 | 1988-04-07 | Agricultural Genetics Co | Antibiotic |
US5055293A (en) * | 1988-03-09 | 1991-10-08 | Purdue Research Foundation | Biological pesticide |
US5045314A (en) * | 1989-11-14 | 1991-09-03 | The United States Of America As Represented By The Secretary Of Agriculture | Control of parasitic nematode ova/larvae with a bacillus laterosporus |
CA2035738C (en) * | 1990-02-07 | 2001-04-24 | Zongling Liu | Biological agent for control of crop fungal disease |
US5187091A (en) * | 1990-03-20 | 1993-02-16 | Ecogen Inc. | Bacillus thuringiensis cryiiic gene encoding toxic to coleopteran insects |
US5208017A (en) * | 1991-02-21 | 1993-05-04 | Mycogen Corporation | Biologically active Bacillus thuringiensis isolates |
JP2948936B2 (en) * | 1991-03-14 | 1999-09-13 | 森永製菓株式会社 | Control method of mold venom produced by mold belonging to genus Fusarium |
GB9206645D0 (en) * | 1992-03-26 | 1992-05-06 | Mini Agriculture & Fisheries | Biological control of post harvest pests |
JP3237240B2 (en) * | 1992-10-27 | 2001-12-10 | 昭和電工株式会社 | Plant disease control agent |
US5849870A (en) * | 1993-03-25 | 1998-12-15 | Novartis Finance Corporation | Pesticidal proteins and strains |
US5733355A (en) * | 1994-09-29 | 1998-03-31 | Susumu Hibino | Bacterial Preparation for agricultural use |
US5552138A (en) * | 1995-06-06 | 1996-09-03 | Wisconsin Alumni Research Foundation | Bacillus cereus strain AS4-12 |
US5645831A (en) * | 1996-03-22 | 1997-07-08 | Biodiscovery New Zealand Ltd. | Bacillus thuringiensis strain and metabolite which are active against corn rootworm |
US5733544A (en) * | 1996-11-18 | 1998-03-31 | University Of Saskatchewan | Nematicidal bacillus strain and metabolite and methods of use thereof |
US5753222A (en) * | 1996-11-18 | 1998-05-19 | Agritope, Inc. | Antibiotic-producing strain of bacillus and methods for controlling plant diseases |
PL198772B1 (en) * | 1997-05-09 | 2008-07-31 | Agraquest | Novel Bacillus strain for fighting against plant diseases and root pests of Diabrotica cereals |
-
1998
- 1998-12-30 US US09/223,587 patent/US6103228A/en not_active Expired - Lifetime
-
1999
- 1999-10-21 CZ CZ20011620A patent/CZ20011620A3/en unknown
- 1999-10-21 WO PCT/US1999/024827 patent/WO2000029426A1/en not_active Application Discontinuation
- 1999-10-21 HU HU0104242A patent/HUP0104242A2/en unknown
- 1999-10-21 IL IL14303799A patent/IL143037A0/en unknown
- 1999-10-21 KR KR1020017006053A patent/KR20010075707A/en not_active Application Discontinuation
- 1999-10-21 CA CA002350782A patent/CA2350782A1/en not_active Abandoned
- 1999-10-21 BR BR9915339-4A patent/BR9915339A/en not_active Application Discontinuation
- 1999-10-21 AU AU12233/00A patent/AU1223300A/en not_active Abandoned
- 1999-10-21 CN CN99815075A patent/CN1335854A/en active Pending
- 1999-10-21 SK SK656-2001A patent/SK6562001A3/en unknown
- 1999-10-21 PL PL99348637A patent/PL348637A1/en unknown
- 1999-10-21 JP JP2000582413A patent/JP2002530290A/en not_active Withdrawn
- 1999-10-21 EP EP99972218A patent/EP1131342A4/en not_active Ceased
-
2000
- 2000-03-01 US US09/516,488 patent/US6417163B1/en not_active Expired - Lifetime
-
2001
- 2001-05-11 MX MXPA01004814 patent/MXPA01004814A/en unknown
- 2001-05-11 NO NO20012344A patent/NO20012344L/en not_active Application Discontinuation
- 2001-11-27 US US09/994,234 patent/US6638910B2/en not_active Expired - Lifetime
Non-Patent Citations (4)
Title |
---|
KIMURA ET AL.: "SNA-60-367, New Peptide Enzyme Inhibitors against Aromatasa", J. ANTIBIOT.,, vol. 50, no. 6, June 1997 (1997-06-01), pages 529 - 531, XP002927239 * |
See also references of EP1131342A4 * |
TSUGE ET AL.: "Characterization of bacillus subtilis YB8, coproducer of pipopeptides surfactin and plipastatin B1", J. GEN. APPL. MICROBIOL.,, vol. 41, no. 6, 1995, pages 541 - 545, XP002927240 * |
YAMADA ET AL.: "Biological Activity of Antifungal Substances produced by Bacillus subtilis", J. PESTIC. SCI.,, vol. 15, no. 1, 1990, pages 95 - 96, XP002927241 * |
Cited By (71)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004002510A1 (en) * | 2002-06-28 | 2004-01-08 | Faculte Universitaire Des Sciences Agronomiques De Gembloux | Novel use of lipopeptide preparations |
US7994138B2 (en) | 2004-06-01 | 2011-08-09 | Agscitech Inc. | Microbial biosurfactants as agents for controlling pests |
US8680060B2 (en) | 2004-06-01 | 2014-03-25 | Agscitech Inc. | Compositions and methods for controlling pests with glycolipids |
US8211828B2 (en) | 2007-01-19 | 2012-07-03 | Basf Se | Fungicidal mixtures of 1-methylpyrazol-4-ylcarboxanilides and azolopyrimidinylamines |
EP2614716A1 (en) | 2007-09-20 | 2013-07-17 | Basf Se | Combinations comprising a fungicidal strain and at least one additional fungicide |
US9078447B2 (en) | 2007-09-20 | 2015-07-14 | Bayer Cropscience Lp | Combinations comprising a fungicidal strain and an active compound |
EP2319319A1 (en) | 2007-09-20 | 2011-05-11 | Basf Se | Combinations comprising a fungicidal strain and at least one additional fungicide |
EP3510869A1 (en) | 2007-09-20 | 2019-07-17 | Bayer Cropscience LP | Combinations comprising a fungicidal strain and an active compound |
EP2614712A1 (en) | 2007-09-20 | 2013-07-17 | Basf Se | Combinations comprising a fungicidal strain and at least one additional fungicide |
AU2014203689B2 (en) * | 2007-09-20 | 2016-02-04 | Bayer Cropscience Lp | Combinations comprising a fungicidal strain and an active compound |
US20150264939A1 (en) * | 2007-09-20 | 2015-09-24 | Bayer Cropscience Lp | Combinations Comprising a Fungicidal Strain and an Active Compound |
US9392797B2 (en) * | 2007-09-20 | 2016-07-19 | Bayer Cropscience Lp | Combinations comprising a fungicidal strain and an active compound |
EP2614715A1 (en) | 2007-09-20 | 2013-07-17 | Basf Se | Combinations comprising a fungicidal strain and at least one additional fungicide |
WO2009037242A3 (en) * | 2007-09-20 | 2010-05-14 | Basf Se | Combinations comprising a fungicidal strain and an active compound |
AU2008300629B2 (en) * | 2007-09-20 | 2014-04-03 | Basf Se | Combinations comprising a fungicidal strain and an active compound |
EA018247B1 (en) * | 2007-09-20 | 2013-06-28 | Басф Се | Combinations comprising a fungicidal strain and an active compound |
WO2009037242A2 (en) * | 2007-09-20 | 2009-03-26 | Basf Se | Combinations comprising a fungicidal strain and an active compound |
EP2614717A1 (en) | 2007-09-20 | 2013-07-17 | Basf Se | Combinations comprising a fungicidal strain and at least one additional fungicide |
EP2614714A1 (en) | 2007-09-20 | 2013-07-17 | Basf Se | Combinations comprising a fungicidal strain and at least one additional fungicide |
EP2614713A1 (en) | 2007-09-20 | 2013-07-17 | Basf Se | Combinations comprising a fungicidal strain and at least one additional fungicide |
EP2754354A1 (en) * | 2008-09-17 | 2014-07-16 | Agraquest, Inc. | Method for using a bacillus subtilis strain to enhance animal health |
WO2010033714A1 (en) | 2008-09-17 | 2010-03-25 | Agraquest, Inc. | Method for using a bacillus subtilis strain to enhance animal health |
AU2009293213B2 (en) * | 2008-09-17 | 2014-10-23 | Bayer Cropscience Lp | Method for using a Bacillus subtilis strain to enhance animal health |
EP2348880A4 (en) * | 2008-09-17 | 2011-11-23 | Agraquest Inc | Method for using a bacillus subtilis strain to enhance animal health |
AU2009293213C1 (en) * | 2008-09-17 | 2015-04-30 | Bayer Cropscience Lp | Method for using a Bacillus subtilis strain to enhance animal health |
EP2348880A1 (en) * | 2008-09-17 | 2011-08-03 | Agraquest, Inc. | Method for using a bacillus subtilis strain to enhance animal health |
US9993004B2 (en) | 2009-05-06 | 2018-06-12 | Bayer Cropscience Lp | Method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure |
US9795138B2 (en) | 2009-05-06 | 2017-10-24 | Bayer Cropscience Lp | Method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure |
US10092008B2 (en) | 2009-05-06 | 2018-10-09 | Bayer Cropscience Lp | Method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure |
US9457054B2 (en) | 2010-03-17 | 2016-10-04 | Bayer Cropscience Lp | Method for using a Bacillus subtilis strain for prophylaxis and treatment of gastro-intestinal conditions |
WO2012130221A2 (en) | 2011-04-01 | 2012-10-04 | Abitep Gmbh | Means for treating microbial diseases in cultivated plants |
DE102011015803A1 (en) | 2011-04-01 | 2012-10-04 | Abitep Gmbh | Agent for the treatment of microbial diseases in crop plants |
ES2402726A1 (en) * | 2011-10-28 | 2013-05-08 | Investigaciones Y Aplicaciones Biotecnologicas, S.L. | New strain of bacillus subtilis for combating plant diseases |
WO2013060910A1 (en) * | 2011-10-28 | 2013-05-02 | Investigaciones Y Aplicaciones Biotecnologicas, S.L. | New strain of bacillus subtilis for combating plant diseases |
US9686999B2 (en) | 2011-10-28 | 2017-06-27 | Investigaciones Y Aplicaciones Biotechnologicas, S.L. | Strain of Bacillus subtilis for combating plant diseases |
CN104010511A (en) * | 2011-11-04 | 2014-08-27 | 拜尔作物科学有限合伙公司 | Biocontrol of nematodes |
US9023339B2 (en) | 2011-11-04 | 2015-05-05 | Bayer Cropscience Lp | Biocontrol of nematodes |
WO2013067275A1 (en) * | 2011-11-04 | 2013-05-10 | Agraquest. Inc. | Biocontrol of nematodes |
WO2013126387A2 (en) * | 2012-02-21 | 2013-08-29 | Dupont Nutrition Biosciences Aps | Composition |
WO2013126387A3 (en) * | 2012-02-21 | 2013-11-07 | Dupont Nutrition Biosciences Aps | Composition comprising fermentation products from bacillus subtilis |
EP3292764A3 (en) * | 2012-05-30 | 2018-04-25 | Bayer CropScience Aktiengesellschaft | Composition comprising a biological control agent and a fungicide selected from inhibitors of the respiratory chain at complex iii |
US9380787B2 (en) | 2012-05-30 | 2016-07-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of amino acid or protein biosynthesis, inhibitors of ATP production and inhibitors of the cell wall synthesis |
US9596860B2 (en) | 2012-05-30 | 2017-03-21 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the mitosis and cell division or compounds having a multi-site action |
US9364006B2 (en) | 2012-05-30 | 2016-06-14 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide |
US9700056B2 (en) | 2012-05-30 | 2017-07-11 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis |
WO2013178650A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the respiratory chain at complex iii |
US9386773B2 (en) | 2012-05-30 | 2016-07-12 | Bayer Cropscience Ag | Compositions comprising a biological control agent and a fungicide from the group consisting of inhibitors of the respiratory chain at complex I or II |
US10076119B2 (en) | 2012-11-22 | 2018-09-18 | Basf Corporation | Pesticidal mixtures |
US11284623B2 (en) | 2012-11-22 | 2022-03-29 | Basf Corporation | Pesticidal mixtures |
EA031644B1 (en) * | 2013-03-20 | 2019-02-28 | Басф Корпорейшн | Synergistic compositions comprising a bacillus subtilis strain and a pesticide |
AU2014233858C1 (en) * | 2013-03-20 | 2018-01-18 | Basf Corporation | Synergistic compositions comprising a Bacillus subtilis strain and a pesticide |
WO2014147534A1 (en) * | 2013-03-20 | 2014-09-25 | Basf Corporation | Synergistic compositions comprising a bacillus subtilis strain and a pesticide |
AU2014233858B2 (en) * | 2013-03-20 | 2017-09-07 | Basf Corporation | Synergistic compositions comprising a Bacillus subtilis strain and a pesticide |
WO2014178032A1 (en) | 2013-05-03 | 2014-11-06 | Universidad Eafit | Production process for biomass and fengycin metabolites of bacillus species and compositions thereof for biological pest control |
EP2871245A1 (en) * | 2013-11-08 | 2015-05-13 | Symborg, S.L. | A bacillus subtilis strain deposited under deposit number CECT 8258 and method for protecting or treating plants |
WO2015067765A1 (en) * | 2013-11-08 | 2015-05-14 | Symborg S.L. | A bacillus subtilis strain deposited under deposit number cect 8258 and method for protecting or treating plants |
US10251400B2 (en) | 2014-05-23 | 2019-04-09 | Basf Se | Mixtures comprising a Bacillus strain and a pesticide |
US11083202B2 (en) | 2014-05-23 | 2021-08-10 | Basf Se | Mixtures comprising a bacillus strain and a pesticide |
US10779536B2 (en) | 2014-11-07 | 2020-09-22 | Basf Se | Pesticidal mixtures |
US10986842B2 (en) | 2015-11-10 | 2021-04-27 | Chr. Hansen A/S | Microbial pesticidal composition and production thereof |
EP3205209A1 (en) | 2016-02-09 | 2017-08-16 | Basf Se | Mixtures and compositions comprising paenibacillus strains or metabolites thereof and other biopesticides |
US11185078B2 (en) | 2016-02-09 | 2021-11-30 | Basf Se | Mixtures and compositions comprising Paenibacillus strains or metabolites thereof and other biopesticides |
EP3205208A1 (en) | 2016-02-09 | 2017-08-16 | Basf Se | Mixtures and compositions comprising paenibacillus strains or fusaricidins and chemical pesticides |
EP4292433A2 (en) | 2016-02-09 | 2023-12-20 | Basf Se | Mixtures and compositions comprising paenibacillus strains or fusaricidins and chemical pesticides |
US10905122B2 (en) | 2016-03-16 | 2021-02-02 | Basf Se | Use of tetrazolinones for combating resistant phytopathogenic fungi on cereals |
US11241012B2 (en) | 2016-03-16 | 2022-02-08 | Basf Se | Use of tetrazolinones for combating resistant phytopathogenic fungi on soybean |
US11425909B2 (en) | 2016-03-16 | 2022-08-30 | Basf Se | Use of tetrazolinones for combating resistant phytopathogenic fungi on fruits |
WO2020084609A1 (en) * | 2018-10-21 | 2020-04-30 | Grace Breeding Ltd. | Dual-route administration of composition for improved protection of plants against pathogens |
WO2020092381A1 (en) * | 2018-10-30 | 2020-05-07 | AgBiome, Inc. | Bacterial compositions and methods for controlling plant pests and improving plant health |
CN113631040A (en) * | 2018-10-30 | 2021-11-09 | 农业生物群落股份有限公司 | Compositions and methods for controlling plant pests and improving plant health |
CN113631040B (en) * | 2018-10-30 | 2023-07-07 | 农业生物群落股份有限公司 | Compositions and methods for controlling plant pests and improving plant health |
Also Published As
Publication number | Publication date |
---|---|
SK6562001A3 (en) | 2001-12-03 |
AU1223300A (en) | 2000-06-05 |
CA2350782A1 (en) | 2000-05-25 |
US20030186852A1 (en) | 2003-10-02 |
PL348637A1 (en) | 2002-06-03 |
US6103228A (en) | 2000-08-15 |
NO20012344L (en) | 2001-06-29 |
CN1335854A (en) | 2002-02-13 |
MXPA01004814A (en) | 2002-10-01 |
BR9915339A (en) | 2001-10-09 |
EP1131342A4 (en) | 2002-03-20 |
US6638910B2 (en) | 2003-10-28 |
JP2002530290A (en) | 2002-09-17 |
NO20012344D0 (en) | 2001-05-11 |
IL143037A0 (en) | 2002-04-21 |
KR20010075707A (en) | 2001-08-09 |
EP1131342A1 (en) | 2001-09-12 |
US6417163B1 (en) | 2002-07-09 |
HUP0104242A2 (en) | 2002-03-28 |
CZ20011620A3 (en) | 2001-09-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6103228A (en) | Compositions and methods for controlling plant pests | |
US6060051A (en) | Strain of bacillus for controlling plant diseases and corn rootworm | |
CA2367775C (en) | A strain of bacillus pumilus for controlling plant diseases | |
MXPA99010078A (en) | A novel strain of bacillus | |
ZA200107386B (en) | A strain of Bacillus pumilus for controlling plant diseases. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: P-327/01 Country of ref document: YU Ref document number: 99815075.4 Country of ref document: CN |
|
ENP | Entry into the national phase |
Ref document number: 2000 12233 Country of ref document: AU Kind code of ref document: A |
|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 143037 Country of ref document: IL Ref document number: 511575 Country of ref document: NZ Ref document number: P20010328A Country of ref document: HR |
|
WWE | Wipo information: entry into national phase |
Ref document number: 200103767 Country of ref document: ZA Ref document number: PV2001-1620 Country of ref document: CZ |
|
ENP | Entry into the national phase |
Ref document number: 2350782 Country of ref document: CA Ref document number: 1999US 9924827 Country of ref document: KE Kind code of ref document: A Ref document number: 2350782 Country of ref document: CA Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1999US9924827 Country of ref document: KE Ref document number: IN/PCT/2001/00401/DE Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: PA/a/2001/004814 Country of ref document: MX Ref document number: 6562001 Country of ref document: SK |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1020017006053 Country of ref document: KR |
|
ENP | Entry into the national phase |
Ref document number: 2000 582413 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: A 2001 00521 Country of ref document: RO |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12233/00 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1999972218 Country of ref document: EP Ref document number: 1200100546 Country of ref document: VN |
|
WWP | Wipo information: published in national office |
Ref document number: 1020017006053 Country of ref document: KR |
|
WWP | Wipo information: published in national office |
Ref document number: 1999972218 Country of ref document: EP Ref document number: PV2001-1620 Country of ref document: CZ |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWR | Wipo information: refused in national office |
Ref document number: 1999972218 Country of ref document: EP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1999972218 Country of ref document: EP |
|
WWR | Wipo information: refused in national office |
Ref document number: PV2001-1620 Country of ref document: CZ |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1020017006053 Country of ref document: KR |