Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/20—Bacteria; Culture media therefor

Definitions

• the present invention relates to a mixed microbial population, a method intended primarily for the biodegradation of fecal material of animals by using it, particularly a biodegradation purification method, and the use of the treated waste water as a liquid manure for plants.
• the present invention is directed to the mixture of microorganisms capable of decomposing livestock and poultry waste materials, including human feces into materials which are more environmentally acceptable and thereby also eliminating odors associated with the decay of them by contacting said materials with said microorganisms, preferably microorganisms in aqueous solution.
• waste materials discharged into bodies of water around the world are rising steadily. Increases in population, the dependence of agriculture on massive amounts of fertilizers and pesticides, expansion of the food industry, and growth of other industrial processes all contribute to the volume of sewage and wastewater and to their content of undesirable substances.
• Most organic waste materials may be degraded by biological treatment. This treatment utilizes the functions of microorganisms to degrade the harmful substances to make them harmless to the environment.
• the use of microorganisms for the treatment of waste water is an economical alternative to physical treatment systems since biological treatment involves lower capital investment, lower energy requirements, a self-sustaining operation, and finally possibility for product recovery.
• the biological process may be achieved aerobically or anaerobically in a number of ways.
• the most widely used aerobic processes are activated sludge processes and their modifications for both domestic and industrial wastewaters.
• the process consists of aerating and agitating the effluent in the presence of a flocculated suspension of microorganisms on particulated organic water-the activated sludge and thereby gives BOD or COD reduction depending on the nature of the wastewater.
• a mixed microbial population degrades the complex mixture of organic compounds in liguid waste to carbonate, nitrate, ammonia, phosphate, and sulfate.
• Major studies on microorganisms which decompose organic sewage and offensive odorous substance are conducted on the sewage treatment by activating sludge method.
• JSB 98.0 a mixed microbial popupation designated as JSB 98.0 having the powerful ability to degrade and deodorize livestock and poultry waste materials, including human feces.
• the first aspect of the present invention is a mixed microbial population designated as JSB 98.0(accession number in Korean Collection for Type Culture(KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), No. KCTC 0524BP).
• the second aspect of the present invention is a biological treating method for a medium polluted with excreta, which comprises the degaradation step of excreta by bringing JSM 98.0 into contact with the medium polluted with excreta.
• the third aspect of the present invention is the use of the treated wastes as a liquid manure for plants.
• the inventors of the present invention screened microorganisms capable of degrading excreta and from the wastewater polluted with excreta from porcine houses a powerful microbial population was obtained which can degrade excreta of high concentration. Thus, a method was found applying this mixed microbial population to the environment containing excreta.
• the isolates has a feature that it maintains relatively high activity to degrade excreta even at a low temperatures such as about 15 ° C .
• the temperature of waste water to be treated is in the range of 10-20 ° C which is lower than usual optimum growth temperatures of microorganisms.
• the microorganisms of the present invention is lower than its optimum growth temperature, but it can maintain the activity to degrade excreta sufficient for practical use.
• the isolated microbial population can also deodorize the offensive odors associated with the decay of excreta when treated to the animal houses, or levatories.
• the method for treating excreta comprises bringing the above-mentioned microbial mixtures, JSB 98.0 into contact with these excreta in an aqueous medium such as waste water.
• the contact of microorganism with these excreta in an aqueous medium is accomplished by culturing the microorganism in the aqueous medium containing excreta or by adding the aqueous medium to the culture system of the microorganism, in a batch mode, a semicontinuous mode or a continuous mode.
• the introduction and exhaust of the gas may be continuously carried out, intermittently or batch-wise in compliance with a treatment performance. These conditions are preferably optimized by controlling the system in accordance with the concentration of the remaing waste materials.
• the fecal material of animals or excreta is treated, in accordance with the present invention, with a mixed microbial population designated as JSB98.0 to form desired liquid manure.
• the excreta may be either fresh or aged matter obtained from animal, particularly livestock and poultry production waste, including human.
• fecal matter of animals such as cows, pigs, horses and the like
• JSB 98.0 can be degraded and deodorized in a liquid by JSB 98.0.
• the mixed microbial population of the present invention, JSB 98.0 can be applied to a biodegradation purification process and environment remediation method which are useful for purification of waste water pulluted with excreta. It can be also useful for the remediation of soil polluted with them.
• the microorganisms of the present invention JSB 98.0 was used as a deodorant.
• the treated wastewater is used as a liquid manure for plants. Since the treated wastes are substantially odor- free, non-air polluting and aesthetically acceptable, they are suitable for use as an inexpensive source of plant nutrients and soil conditioner for the production of flowers, lawns, shrubs, field crops, pastures and the like.
• the present invention comprises (a)isolating microbial strains from sources and identifying the potential strains which have the powerful ability to decompose the fecal material of animals (b)treating said fecal materials(or waste water polluted with them) with said isolates (c)Assessing the treated waste water (d)controlling and eliminating the offensive odors from places containing said fecal materials with said isolates and (e)using the said treated waste water as a liguid manure for plants.
• Microorganisms have been isolated from the porcine houses. Among all the isolates, 5 active and predominant strains were identified as Corynebacterium glutamicum HO777, Micrococcus kristinae HO778, Paracoccus denitrificans HO779, Corynebacterium glutamicum HO780 and Bacillus mycoides HO781 , respectively. On the basis of the results of identification study of the microorganism, the present inventors designated the mixed microbial population of the 5 active strains as JSB 98.0.
• More than 13 strains formed colonies on the agar count plates. The total number of bacterial cells on the plates was measured and 5 bacterial strains which revealed 10% of the total microbial population in number were selected for further study. The selected colonies are as follows.
• Strain 1 microcolony with cream colony, 20% Strain 2 : white and sticky colony, 18% Strain 3 : yellow colony, 14% Strain 4 : plane brownish colony, 11% Strain 5 : rhizoid from colony, 10%
• This bacteria was designated as strain HO778
• Gram stain positive Shape and size of cell : spherical, 0.5-1 .1 ⁇ m in diameter mostly 4 cells in clusters or irregular clusters Colony : round, entire, convex, smooth, pale creamy in color
• Shape and size of cell short rod or elipsoid, 0.7-1.0 X 1.0-3.0 ⁇ m mostly elipsoidal sphere during late phase of growth, single, pairs or irregular clusters
• Urease production positive Casein decomposition : negative Gelatin decomposition : negative Nitrate reduction Asimilation test : D-ribose, D-xylose, acetic acid, propionic acid (Biolog
• Shape and size of cell rod or elipsoidal sphere
• Urease positive Nitrate reduction : positive Casein decomposition : negative Gelatin decomposition : negative Asimilation test : ⁇ -cyclodextrin, cellobiose, D-fructose, tT -D-glucose, maltotriose, D-mannose, 3-methyl glucose, palatinose, D-psicose, D-ribose, sucrose, turanose, D-xylose, acetic acid, L-malic acid, L-asparagine, L-glutamic acid, uridine(Biolog GP) (3) Fatty acids composition in cell membrane
• the strain was identified as Corynebacterium glutamicum (confidence level 99%)
• Size and shape of cell sphere, 0.5-0.9 ⁇ m in diameter or short rod, 0.9-1.2 ⁇ m in length single, pairs or clusters
• Nitric acid reduction in anaerobic condition positive(nitrogen oxides or nitrogen)
• Gelatin decomposition negative Starch hydrolysis : negative Asimilation test : L-ribose, D-ribose, D-xylose, ⁇ -hydroxybutyric acid, D-lactic acid methyl ester, L-lactic acid, methyl pyruvate, mono-methyl succinate
• the strain was identified as Paracoccus denitrificans (confidence level 85%)
• strain HO781 This bacteria was designated as strain HO781
• Gram stain positive Size and shape of cell : rod in chains Colony : rhizoid, frosted glass apperance Spore : elipsoidal , central
• the strain was identified as Bacillus mycoides (confidence level 90%)
• Feedlot waste from a cattle feedlot was collected in an ordinary waste water treating tank and JSB 98.0 was inoculated to determine the presence of microbial activity.
• the aeration time was maintained 18 hours/day and the dissolved oxygen concentration was maintained as high as about 2ppm. This example was performed three times repeatedly from June 28 to July 6, 1998. The result is shown in table 1.
• JSB 98.0 of the present invention was highly effective to decrease BOD level.
• the present invention has provided a method for the treatment of livestock and poultry waste for the purpose of assisting in the abatement and management of water pollution caused by those animal excreta materials.
• JSB 98.0 the chemical properties of the waste water polluted with excreta have been significantly improved.
• novel mixed microbial population of the present invention designated as JSB 98.0 which can degrade fecal material of animals, including humans enables the biological degradation of these materials utilizing its growth chacteristics different from those of conventional single species, thus efficient biological treatment of waste water containing these materials.

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Health & Medical Sciences (AREA)
• Genetics & Genomics (AREA)
• Biotechnology (AREA)
• Organic Chemistry (AREA)
• Zoology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Wood Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• Microbiology (AREA)
• Biomedical Technology (AREA)
• Virology (AREA)
• Biochemistry (AREA)
• General Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Tropical Medicine & Parasitology (AREA)
• Micro-Organisms Or Cultivation Processes Thereof (AREA)
• Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
• Treatment Of Sludge (AREA)
• Fertilizers (AREA)

Priority Applications (3)

Applications Claiming Priority (2)

Publications (1)

Family

ID=19556491

Family Applications (1)

Country Status (6)

Cited By (2)

Families Citing this family (6)

Citations (4)

• 1998
  • 1998-10-30 KR KR1019980046275A patent/KR100298297B1/ko not_active IP Right Cessation
• 1999
  • 1999-10-30 EP EP99951247A patent/EP1124942A1/en not_active Withdrawn
  • 1999-10-30 WO PCT/KR1999/000652 patent/WO2000026341A1/en not_active Application Discontinuation
  • 1999-10-30 JP JP2000579713A patent/JP2002528113A/ja active Pending
  • 1999-10-30 CN CNB998151769A patent/CN1177034C/zh not_active Expired - Fee Related
  • 1999-10-30 AU AU63716/99A patent/AU6371699A/en not_active Abandoned

Patent Citations (4)

Non-Patent Citations (2)

Also Published As

Similar Documents

Legal Events