WO2000024928A3 - Ein automatisierbarer schnelltest zum direkten nachweis der apc resistenz-mutation mit spezifischen primern und oligonukleotiden zur detektion - Google Patents

Ein automatisierbarer schnelltest zum direkten nachweis der apc resistenz-mutation mit spezifischen primern und oligonukleotiden zur detektion Download PDF

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Publication number
WO2000024928A3
WO2000024928A3 PCT/EP1999/007609 EP9907609W WO0024928A3 WO 2000024928 A3 WO2000024928 A3 WO 2000024928A3 EP 9907609 W EP9907609 W EP 9907609W WO 0024928 A3 WO0024928 A3 WO 0024928A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
automatable
oligonucleotides
resistance mutation
specific primers
Prior art date
Application number
PCT/EP1999/007609
Other languages
English (en)
French (fr)
Other versions
WO2000024928A2 (de
Inventor
Wolfgang Springer
Original Assignee
Bayer Ag
Wolfgang Springer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag, Wolfgang Springer filed Critical Bayer Ag
Priority to AU63376/99A priority Critical patent/AU6337699A/en
Priority to EP99950696A priority patent/EP1121467A2/de
Publication of WO2000024928A2 publication Critical patent/WO2000024928A2/de
Publication of WO2000024928A3 publication Critical patent/WO2000024928A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft einen automatisierbaren Gentest zum Nachweis des Thromboserisikofaktors APC (aktiviertes Protein C), geeignete Starteroligonukleotide, Oligonukleotidsonden für diesen Gentest, sowie ein Verfahren und ein Testkit zum Nachweis der APC-Resistenz-Mutation.
PCT/EP1999/007609 1998-10-22 1999-10-11 Ein automatisierbarer schnelltest zum direkten nachweis der apc resistenz-mutation mit spezifischen primern und oligonukleotiden zur detektion WO2000024928A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU63376/99A AU6337699A (en) 1998-10-22 1999-10-11 Automatable quick test for direct detection of apc resistance mutation with specific primers and assay
EP99950696A EP1121467A2 (de) 1998-10-22 1999-10-11 Ein automatisierbarer schnelltest zum direkten nachweis der apc resistenz mutation mit spezifischen primern und oligonukleotiden zur detektion

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19848665.0 1998-10-22
DE19848665A DE19848665A1 (de) 1998-10-22 1998-10-22 Ein automatisierbarer Schnelltest zum direkten Nachweis der APC Resistenz Mutation mit spezifischen Primern und Probes

Publications (2)

Publication Number Publication Date
WO2000024928A2 WO2000024928A2 (de) 2000-05-04
WO2000024928A3 true WO2000024928A3 (de) 2000-06-22

Family

ID=7885260

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/007609 WO2000024928A2 (de) 1998-10-22 1999-10-11 Ein automatisierbarer schnelltest zum direkten nachweis der apc resistenz-mutation mit spezifischen primern und oligonukleotiden zur detektion

Country Status (4)

Country Link
EP (1) EP1121467A2 (de)
AU (1) AU6337699A (de)
DE (1) DE19848665A1 (de)
WO (1) WO2000024928A2 (de)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0200362A2 (de) * 1985-03-28 1986-11-05 F. Hoffmann-La Roche Ag Verfahren zur Amplifikation, zum Nachweis und/oder zur Klonierung von Nukleinsäuresequenzen
WO1992013103A1 (en) * 1991-01-16 1992-08-06 The Johns Hopkins University Inherited and somatic mutations of apc gene in colorectal cancer of humans
WO1995021938A1 (en) * 1994-02-14 1995-08-17 Rijks Universiteit Leiden A method for screening for the presence of a genetic defect associated with thrombosis and/or poor anticoagulant response to activated protein c
WO1996030546A1 (en) * 1995-03-24 1996-10-03 The Scripps Research Institute Methods for identifying a factor v gene mutation
EP0787989A2 (de) * 1996-01-30 1997-08-06 IMMUNO Aktiengesellschaft Verfahren zur Herstellung eines standardisierten Plasmapools für diagnostische Zwecke
WO1998033940A1 (en) * 1997-01-31 1998-08-06 The Johns Hopkins University An apc mutation associated with familial colorectal cancer in ashkenazi jews

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0200362A2 (de) * 1985-03-28 1986-11-05 F. Hoffmann-La Roche Ag Verfahren zur Amplifikation, zum Nachweis und/oder zur Klonierung von Nukleinsäuresequenzen
WO1992013103A1 (en) * 1991-01-16 1992-08-06 The Johns Hopkins University Inherited and somatic mutations of apc gene in colorectal cancer of humans
WO1995021938A1 (en) * 1994-02-14 1995-08-17 Rijks Universiteit Leiden A method for screening for the presence of a genetic defect associated with thrombosis and/or poor anticoagulant response to activated protein c
WO1996030546A1 (en) * 1995-03-24 1996-10-03 The Scripps Research Institute Methods for identifying a factor v gene mutation
EP0787989A2 (de) * 1996-01-30 1997-08-06 IMMUNO Aktiengesellschaft Verfahren zur Herstellung eines standardisierten Plasmapools für diagnostische Zwecke
WO1998033940A1 (en) * 1997-01-31 1998-08-06 The Johns Hopkins University An apc mutation associated with familial colorectal cancer in ashkenazi jews

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BEAUCHAMP N J ET AL.: "Rapid two-stage PCR for detecting factor V G1691A mutation", THE LANCET, vol. 344, 1994, pages 694 - 695, XP002132193 *
BERTINA R M ET AL: "MUTATION IN BLOOD COAGULATION FACTOR V ASSOCIATED WITH RESISTANCE TO ACTIVATED PROTEIN C", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 369, no. 6475, 5 May 1994 (1994-05-05), pages 64 - 67, XP000563812, ISSN: 0028-0836 *
ENGEL H ET AL.: "Phenotyping and genotyping of coagulation factor V Leiden", THROMBOSIS AND HAEMOSTASIS, vol. 75, no. 2, 1996, pages 267 - 269, XP000879268 *
GUILLERM C ET AL.: "Rapid detection of the G1691A mutation of coagulation factor V by PCR-mediated site-directed mutagenesis", CLINICAL CHEMISTY, vol. 42, no. 2, 1996, pages 329, XP002132194 *
LEWANDOWSKI K ET AL.: "An alternative method for identifying the factor V gene Leiden mutation", THROMBOSIS RESEARCH, vol. 85, no. 2, 1997, pages 105 - 113, XP000879252 *
PECHENIUK N M ET AL.: "Use of first nucleotide change technology to determine the frequency of factor V Leiden in a population of Australian blood donors", BLOOD COAGULATION AND FIBRINOLYSIS, vol. 8, 1997, pages 491 - 495, XP000879284 *
REITSMA P H ET AL.: "Use of the direct RNA amplification technique NASBA to detect factor V Leiden, a point mutation associated with APC resistance", BLOOD COAGULATION AND FIBROLYSIS, vol. 7, 1996, pages 659 - 663, XP000879262 *
YANDAVA C N ET AL.: "ARMS test for diagnosis of factor V Leiden mutation, a common cause of inherited thrombotic tendency", JOURNAL OF CLINICAL LABORATORY ANALYSIS, vol. 10, 1996, pages 414 - 417, XP000879292 *
ZOTZ R B ET AL.: "Mutation in the gene coding for coagulation factor V and resistance to activated protein C: Detection of the genetic mutation by oligonucleotide ligation assay using a semi-automated system", THROMBOSIS AND HAEMOSTASIS, vol. 76, no. 1, 1996, pages 53 - 55, XP000879265 *

Also Published As

Publication number Publication date
DE19848665A1 (de) 2000-04-27
WO2000024928A2 (de) 2000-05-04
AU6337699A (en) 2000-05-15
EP1121467A2 (de) 2001-08-08

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