WO2000020616A1 - ELICITEUR DE REPONSE HYPERSENSIBLE EXTRAIT DE $i(XANTHOMONAS CAMPESTRIS) - Google Patents

ELICITEUR DE REPONSE HYPERSENSIBLE EXTRAIT DE $i(XANTHOMONAS CAMPESTRIS) Download PDF

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Publication number
WO2000020616A1
WO2000020616A1 PCT/US1999/023265 US9923265W WO0020616A1 WO 2000020616 A1 WO2000020616 A1 WO 2000020616A1 US 9923265 W US9923265 W US 9923265W WO 0020616 A1 WO0020616 A1 WO 0020616A1
Authority
WO
WIPO (PCT)
Prior art keywords
plants
hypersensitive response
plant
response elicitor
protein
Prior art date
Application number
PCT/US1999/023265
Other languages
English (en)
Inventor
Zhong-Min Wei
Shane Swanson
Hao Fan
Original Assignee
Eden Bioscience Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eden Bioscience Corporation filed Critical Eden Bioscience Corporation
Priority to IL14243399A priority Critical patent/IL142433A0/xx
Priority to HU0103789A priority patent/HUP0103789A2/hu
Priority to MXPA01003464A priority patent/MXPA01003464A/es
Priority to AU62928/99A priority patent/AU6292899A/en
Priority to JP2000574710A priority patent/JP2003529530A/ja
Priority to CA002346327A priority patent/CA2346327A1/fr
Priority to EP99950223A priority patent/EP1119632A1/fr
Publication of WO2000020616A1 publication Critical patent/WO2000020616A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Definitions

  • the present invention relates to a Xanthomonas campestris from hypersensitive response elicitor.
  • the method of the present invention involving application of the hypersensitive response elicitor polypeptide or protein can be carried out through a variety of procedures when all or part of the plant is treated, including leaves, stems, roots, propagules (e.g., cuttings), etc. This may (but need not) involve infiltration of the hypersensitive response elicitor polypeptide or protein into the plant.
  • Suitable application methods include high or low pressure spraying, injection, and leaf abrasion proximate to when elicitor application takes place.
  • the DNA molecule may also be introduced into the plant cells by electroporation. Fromm et al., Proc. Natl. Acad. Sci. USA, 82:5824 (1985), which is hereby incorporated by reference. In this technique, plant protoplasts are electroporated in the presence of plasmids containing the expression cassette. Electrical impulses of high field strength reversibly permeabilize biomembranes allowing the introduction of the plasmids. Electroporated plant protoplasts reform the cell wall, divide, and regenerate.
  • the purified Xcp hypersensitive response elicitor protein was run on a SDS-PAGE. From this gel, the protein was transferred to a PVDF membrane via a Trans-Blot (Bio-Rad, Hercules, CA). The membrane containing the protein was subjected to an automated Edmans Degradation process followed by high-pressure liquid chromatography for detection and identification of the individual amino acids. The first ten amino acids of the Xcp hypersensitive response elicitor were determined to be:

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne une protéine ou un polypeptide isolé extrait de Xanthomonas campestris déclenchant une réponse hypersensible. Les protéines ou polypeptides déclenchant une réponse hypersensible selon la présente invention, ainsi que les molécules isolées d'ADN les codant peuvent être utilisées pour conférer aux plantes une résistance aux maladies; pour accélérer leur croissance, et/ou éliminer les insectes qui les envahissent. Pour cela, on applique l'éliciteur de réponse hypersensible sous forme non infectieuse sur les plantes ou les graines, dans des conditions efficaces, de façon à conférer aux plantes ou au plantes issues des graines une résistance aux maladies, à accélérer leur croissance, et/ou éliminer les insectes qui les envahissent. Selon un autre mode de réalisation, l'invention concerne la production de plantes ou de graines transgéniques transformées par une molécule d'ADN codant pour l'éliciteur. On cultive alors les plantes transgéniques ou les plantes issues de graines transgéniques dans des conditions efficaces, de manière à conférer aux plantes ou aux plantes issues des graines une résistance aux maladies, à accélérer leur croissance, et/ou à éliminer les insectes qui les envahissent.
PCT/US1999/023265 1998-10-05 1999-10-05 ELICITEUR DE REPONSE HYPERSENSIBLE EXTRAIT DE $i(XANTHOMONAS CAMPESTRIS) WO2000020616A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
IL14243399A IL142433A0 (en) 1998-10-05 1999-10-05 Hypersensitive response elicitor from xanthomonas campestris
HU0103789A HUP0103789A2 (hu) 1998-10-05 1999-10-05 A Xanthomonas campestrisből származó, hiperszenzitív választ kiváltó anyag
MXPA01003464A MXPA01003464A (es) 1998-10-05 1999-10-05 Promotor de la respuesta hipersensible de xanthomonas campestris.
AU62928/99A AU6292899A (en) 1998-10-05 1999-10-05 Hypersensitive response elicitor from (xanthomonas campestris)
JP2000574710A JP2003529530A (ja) 1998-10-05 1999-10-05 キサントモナス・カンペストリスに由来する過敏感反応エリシター
CA002346327A CA2346327A1 (fr) 1998-10-05 1999-10-05 Eliciteur de reponse hypersensible extrait de xanthomonas campestris
EP99950223A EP1119632A1 (fr) 1998-10-05 1999-10-05 Eliciteur de reponse hypersensible extrait de xanthomonas campestris

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10312498P 1998-10-05 1998-10-05
US60/103,124 1998-10-05

Publications (1)

Publication Number Publication Date
WO2000020616A1 true WO2000020616A1 (fr) 2000-04-13

Family

ID=22293528

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/023265 WO2000020616A1 (fr) 1998-10-05 1999-10-05 ELICITEUR DE REPONSE HYPERSENSIBLE EXTRAIT DE $i(XANTHOMONAS CAMPESTRIS)

Country Status (9)

Country Link
EP (1) EP1119632A1 (fr)
JP (1) JP2003529530A (fr)
AU (1) AU6292899A (fr)
CA (1) CA2346327A1 (fr)
HU (1) HUP0103789A2 (fr)
IL (1) IL142433A0 (fr)
MX (1) MXPA01003464A (fr)
WO (1) WO2000020616A1 (fr)
ZA (1) ZA200102711B (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002037960A2 (fr) * 2000-11-13 2002-05-16 Eden Bioscience Corporation Procedes d'inhibition de dessiccation de coupes prelevees sur des plantes ornementales
US7041876B2 (en) 2000-01-26 2006-05-09 Cornell Research Foundation, Inc. Oomycete-resistant transgenic plants by virtue of pathogen-induced expression of a heterologous hypersensitive response elicitor
EP1696725A2 (fr) * 2003-12-23 2006-09-06 Nitragin, Inc. Methodes et compositions produisant sur les legumineuses et les non-legumineuses des effets benefiques sur le plan agronomique
US7915217B2 (en) 2000-04-19 2011-03-29 Plant Health Care, Inc. Treatment of fruits or vegetables with hypersensitive response elicitor to inhibit postharvest disease or desiccation
WO2012001329A2 (fr) 2010-07-02 2012-01-05 Centre National De La Recherche Scientifique - Cnrs Utilisation d'un extrait naturel de marc de raisin pour stimuler les defenses naturelles de plantes
WO2013186303A1 (fr) * 2012-06-13 2013-12-19 Eberhard Karls Universität Tübingen Récepteur de reconnaissance d'un modèle de plante et ses chimères destinés à être utilisés contre des infections bactériennes

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6035605B2 (ja) * 2012-03-16 2016-11-30 国立研究開発法人農業・食品産業技術総合研究機構 非病原性キサントモナス属細菌菌株及び該菌株を用いた微生物農薬

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998024297A1 (fr) * 1996-12-05 1998-06-11 Cornell Research Foundation, Inc. Traitement de graines conferant a des plantes une resistance induite par une reaction d'hypersensibilite
WO1998032844A1 (fr) * 1997-01-27 1998-07-30 Cornell Research Foundation, Inc. Stimulation de la croissance vegetale
WO1998037752A1 (fr) * 1997-02-28 1998-09-03 Cornell Research Foundation, Inc. Lutte contre les insectes a l'aide d'un eliciteur de reponse hypersensible

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998024297A1 (fr) * 1996-12-05 1998-06-11 Cornell Research Foundation, Inc. Traitement de graines conferant a des plantes une resistance induite par une reaction d'hypersensibilite
WO1998032844A1 (fr) * 1997-01-27 1998-07-30 Cornell Research Foundation, Inc. Stimulation de la croissance vegetale
WO1998037752A1 (fr) * 1997-02-28 1998-09-03 Cornell Research Foundation, Inc. Lutte contre les insectes a l'aide d'un eliciteur de reponse hypersensible

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SWANSON, S. ET AL.: "Isolation and characterization of an HR elicitor from Xanthomonas campestris.", PHYTOPATHOLOGY, vol. 88, no. 9 SUPPL., September 1998 (1998-09-01), pages S87, XP000866309 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7041876B2 (en) 2000-01-26 2006-05-09 Cornell Research Foundation, Inc. Oomycete-resistant transgenic plants by virtue of pathogen-induced expression of a heterologous hypersensitive response elicitor
US7915217B2 (en) 2000-04-19 2011-03-29 Plant Health Care, Inc. Treatment of fruits or vegetables with hypersensitive response elicitor to inhibit postharvest disease or desiccation
WO2002037960A2 (fr) * 2000-11-13 2002-05-16 Eden Bioscience Corporation Procedes d'inhibition de dessiccation de coupes prelevees sur des plantes ornementales
WO2002037960A3 (fr) * 2000-11-13 2003-01-30 Eden Bioscience Corp Procedes d'inhibition de dessiccation de coupes prelevees sur des plantes ornementales
EP1696725A2 (fr) * 2003-12-23 2006-09-06 Nitragin, Inc. Methodes et compositions produisant sur les legumineuses et les non-legumineuses des effets benefiques sur le plan agronomique
EP1696725B1 (fr) * 2003-12-23 2015-06-03 Novozymes BioAg, Inc. Methodes et compositions produisant sur les legumineuses et les non-legumineuses des effets benefiques sur le plan agronomique
WO2012001329A2 (fr) 2010-07-02 2012-01-05 Centre National De La Recherche Scientifique - Cnrs Utilisation d'un extrait naturel de marc de raisin pour stimuler les defenses naturelles de plantes
WO2013186303A1 (fr) * 2012-06-13 2013-12-19 Eberhard Karls Universität Tübingen Récepteur de reconnaissance d'un modèle de plante et ses chimères destinés à être utilisés contre des infections bactériennes
US10676756B2 (en) 2012-06-13 2020-06-09 Eberhard Karls Universitaet Tuebingen Plant pattern recognition receptor and chimeras thereof for use against bacterial infections

Also Published As

Publication number Publication date
MXPA01003464A (es) 2002-05-06
IL142433A0 (en) 2002-03-10
CA2346327A1 (fr) 2000-04-13
EP1119632A1 (fr) 2001-08-01
ZA200102711B (en) 2001-11-06
HUP0103789A2 (hu) 2002-01-28
JP2003529530A (ja) 2003-10-07
AU6292899A (en) 2000-04-26

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