WO2000018950A3 - Assay method for ntp hydrolising enzymes - Google Patents

Assay method for ntp hydrolising enzymes Download PDF

Info

Publication number
WO2000018950A3
WO2000018950A3 PCT/US1999/021365 US9921365W WO0018950A3 WO 2000018950 A3 WO2000018950 A3 WO 2000018950A3 US 9921365 W US9921365 W US 9921365W WO 0018950 A3 WO0018950 A3 WO 0018950A3
Authority
WO
WIPO (PCT)
Prior art keywords
assay method
ntp
enzymes
hydrolising
present
Prior art date
Application number
PCT/US1999/021365
Other languages
French (fr)
Other versions
WO2000018950A2 (en
Inventor
Anthony R Welch
Original Assignee
Bioqual Inc
Anthony R Welch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioqual Inc, Anthony R Welch filed Critical Bioqual Inc
Priority to AU60444/99A priority Critical patent/AU6044499A/en
Publication of WO2000018950A2 publication Critical patent/WO2000018950A2/en
Publication of WO2000018950A3 publication Critical patent/WO2000018950A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is drawn to a high throughput assay method of monitoring the activity of enzymes which hydrolyze NTPs as cosubstrates by forcing the reaction to run in the metabolically reverse direction and quantitating the NTP synthesized by the target enzyme. The present invention is further drawn to assay systems which use the assay method.
PCT/US1999/021365 1998-09-25 1999-09-17 Assay method for ntp hydrolising enzymes WO2000018950A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU60444/99A AU6044499A (en) 1998-09-25 1999-09-17 High throughput assay method for enzymes which metabolically hydrolyze nucleoside triphosphates and an assay system therefor

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10180298P 1998-09-25 1998-09-25
US60/101,802 1998-09-25
US11472098P 1998-12-30 1998-12-30
US60/114,720 1998-12-30

Publications (2)

Publication Number Publication Date
WO2000018950A2 WO2000018950A2 (en) 2000-04-06
WO2000018950A3 true WO2000018950A3 (en) 2000-07-27

Family

ID=26798647

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/021365 WO2000018950A2 (en) 1998-09-25 1999-09-17 Assay method for ntp hydrolising enzymes

Country Status (2)

Country Link
AU (1) AU6044499A (en)
WO (1) WO2000018950A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108330160A (en) * 2018-04-18 2018-07-27 易尚明天科技有限公司 The active detection methods of nucleoside diphosphokinase NDPK
EP3752163A4 (en) * 2018-06-14 2021-04-07 National Institute Of Biological Sciences, Beijing Promoting immune responses
CN109182564B (en) * 2018-08-24 2021-05-25 上海芯超医学检验所有限公司 Method for detecting and analyzing toxicity and pathogenicity of helicobacter pylori

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2931378A1 (en) * 1979-08-02 1981-02-19 Schrader Juergen Priv Doz Dr M Determn. of adenosine in blood and tissue using adenosine kinase - To convert adenosine to atp and luciferase-luciferin reaction to determine atp concn. by bio:luminescence
FR2699930A1 (en) * 1992-12-24 1994-07-01 Kabore Paul Bioluminescent determn of adenosine phosphates
WO1994017198A1 (en) * 1993-01-22 1994-08-04 Regents Of The University Of Minnesota Enzymatic fluorometric assay for adenylate cyclase
EP0794260A1 (en) * 1996-03-04 1997-09-10 Kikkoman Corporation Bioluminescence reagent and method for quantitative determination of adenosine phosphate ester using the reagent
US5741635A (en) * 1996-01-30 1998-04-21 Mount Sinai Hospital Corporation Method of quantitating GTP and GDP bound to a G protein and uses thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2931378A1 (en) * 1979-08-02 1981-02-19 Schrader Juergen Priv Doz Dr M Determn. of adenosine in blood and tissue using adenosine kinase - To convert adenosine to atp and luciferase-luciferin reaction to determine atp concn. by bio:luminescence
FR2699930A1 (en) * 1992-12-24 1994-07-01 Kabore Paul Bioluminescent determn of adenosine phosphates
WO1994017198A1 (en) * 1993-01-22 1994-08-04 Regents Of The University Of Minnesota Enzymatic fluorometric assay for adenylate cyclase
US5741635A (en) * 1996-01-30 1998-04-21 Mount Sinai Hospital Corporation Method of quantitating GTP and GDP bound to a G protein and uses thereof
EP0794260A1 (en) * 1996-03-04 1997-09-10 Kikkoman Corporation Bioluminescence reagent and method for quantitative determination of adenosine phosphate ester using the reagent

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BERGMEYER H.U.: "METHODS OF ENZYMATIC ANALYSIS. ENZYMES 1: OXIDOREDUCTASES, TRANSFERASES.", vol. VOL. 3, 1 January 1983, WEINHEIM, VERLAG CHEMIE., DE, pages: 525 - 530., XP002126999, 014042 *

Also Published As

Publication number Publication date
WO2000018950A2 (en) 2000-04-06
AU6044499A (en) 2000-04-17

Similar Documents

Publication Publication Date Title
WO1997040141A3 (en) Isolation of enzymes
FR2772789B1 (en) PROCESS FOR THE ENZYMATIC PREPARATION OF HOMOGENTISATE
EP0952221A3 (en) L-glutamic acid-producing bacterium and method for producing l-glutamic acid
EP0821058A3 (en) Method for enhancing enzyme activity at elevated temperature
FR2708286B1 (en) Method of identifying microorganisms with at least two chromogens.
EP0976829A3 (en) Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof
AU6943696A (en) Process for producing d-pantoic acid and d-pantothenic acid or salts thereof
US5179014A (en) Process for the preparation of amides using microorganisms
WO2003000913A3 (en) Method for the fermentative production of pyruvate
EP0358428B1 (en) Process for producing optically active compounds
JPH04321680A (en) Biosynthetic method of manufacturing 6(r)-(2-(8(s)- hydroxy-2-(s),6(r)-dimethyl-1,2,6,7,8,8a(r)-hexahydro- naphthyl)-ethyl)4(r)-hydroxy-3,4,5,6-tetrahydro-2h-pyran- 2-one triol acid by enzymatic hydrolysis of robastatinic acid by using enzyme obtained from chronostakis compactiuscula
WO2000018950A3 (en) Assay method for ntp hydrolising enzymes
GB2042531A (en) Process for preparing D-???-amino acids
NZ508404A (en) Alcoholic fermentation using a mineral-enriched yeast as a nutrient source
EP0896057A3 (en) D-aminoacylase
AU9560198A (en) Process for the preparation of a beta-lactam antibiotic
US4316955A (en) Enzymatic deesterification of cephalosporin methyl esters
CA2018562A1 (en) Process for the enzymatic cleavage of vinyl 2-aryl-propionates
Kato et al. Maleate cis-trans isomerase from Arthrobacter sp. TPU 5446
PL343524A1 (en) Novel chromogenous substrates for detecting bacterial hydrolase
EP0927755A3 (en) Haloaccloacetate reductase, method for producing said enzyme, and method for producing alcohols using said enzyme
CA2287560A1 (en) Stereoselective microbial reduction of a racemic tetralone
IL70833A (en) Enzymatic conversion of water-insoluble organic substrates
Kim et al. Adsorptive Removal of Inhibitory by-Product in the enzymatic production of optically active D-p-hydroxyphenylglycine from 5-substituted hydantoin
EP1290209B1 (en) Method for preparing an r- or s-form of alpha-substituted heterocyclic carboxylic acid and a counter enantiomeric form of alpha-substituted heterocyclic carboxylic acid ester thereto using enzyme

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase