WO2000008131A8 - Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production - Google Patents

Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production

Info

Publication number
WO2000008131A8
WO2000008131A8 PCT/US1999/017702 US9917702W WO0008131A8 WO 2000008131 A8 WO2000008131 A8 WO 2000008131A8 US 9917702 W US9917702 W US 9917702W WO 0008131 A8 WO0008131 A8 WO 0008131A8
Authority
WO
WIPO (PCT)
Prior art keywords
retroviruses
pseudotyped
cells
glycoproteins
methods
Prior art date
Application number
PCT/US1999/017702
Other languages
English (en)
Other versions
WO2000008131A2 (fr
WO2000008131A3 (fr
WO2000008131A9 (fr
Inventor
David A Sanders
Richard John Kuhn
Scott A Jeffers
Curtis Matthew Sharkey
Cynthia Lin North
Michael A Fischbach
Original Assignee
Purdue Research Foundation
David A Sanders
Richard John Kuhn
Scott A Jeffers
Curtis Matthew Sharkey
Cynthia Lin North
Michael A Fischbach
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, David A Sanders, Richard John Kuhn, Scott A Jeffers, Curtis Matthew Sharkey, Cynthia Lin North, Michael A Fischbach filed Critical Purdue Research Foundation
Priority to US09/762,224 priority Critical patent/US7033595B1/en
Priority to AU54658/99A priority patent/AU5465899A/en
Publication of WO2000008131A2 publication Critical patent/WO2000008131A2/fr
Publication of WO2000008131A3 publication Critical patent/WO2000008131A3/fr
Publication of WO2000008131A9 publication Critical patent/WO2000008131A9/fr
Publication of WO2000008131A8 publication Critical patent/WO2000008131A8/fr

Links

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13061—Methods of inactivation or attenuation
    • C12N2740/13062—Methods of inactivation or attenuation by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/14011—Filoviridae
    • C12N2760/14111—Ebolavirus, e.g. Zaire ebolavirus
    • C12N2760/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des cellules produisant de nouveaux rétrovirus pseudotypés possédant un large spectre d'activité. Dans un aspect de l'invention, les cellules produisent des rétrovirus pseudotypés avec au moins deux glycoprotéines virales différentes, telles que des glycoprotéines togavirales. Dans d'autres modes de réalisation, les cellules produisent des rétrovirus pseudotypés avec des glycoprotéines filovirales. L'invention concerne également des méthodes de production des cellules décrites ci-dessus, ainsi que des rétrovirus pseudotypés ainsi produits. Dans d'autres modes de réalisation encore, l'invention concerne des méthodes de criblage d'agents efficaces pour le blocage d'une entrée virale dans une cellule, y compris un entrée filovirale ou une entrée de virus possédant au moins deux glycoprotéines virales différentes disposées dans leur bicouche lipidique, tels que des togavirus. L'invention concerne en outre des méthodes d'utilisation des rétrovirus pseudotypés selon l'invention pour introduire des séquences nucléotidiques dans des cellules cibles, ainsi que des trousses de formation des rétrovirus pseudotypés selon l'invention.
PCT/US1999/017702 1998-08-04 1999-08-04 Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production WO2000008131A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/762,224 US7033595B1 (en) 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production
AU54658/99A AU5465899A (en) 1998-08-04 1999-08-04 Pseudotyped retroviruses and stable cell lines for their production

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9524298P 1998-08-04 1998-08-04
US60/095,242 1998-08-04
US11240598P 1998-12-15 1998-12-15
US60/112,405 1998-12-15

Publications (4)

Publication Number Publication Date
WO2000008131A2 WO2000008131A2 (fr) 2000-02-17
WO2000008131A3 WO2000008131A3 (fr) 2000-06-02
WO2000008131A9 WO2000008131A9 (fr) 2000-08-03
WO2000008131A8 true WO2000008131A8 (fr) 2001-01-04

Family

ID=26789991

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/017702 WO2000008131A2 (fr) 1998-08-04 1999-08-04 Retrovirus pseudotypes et lignees cellulaires stables destinees a leur production

Country Status (2)

Country Link
AU (1) AU5465899A (fr)
WO (1) WO2000008131A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001253728A1 (en) * 2000-04-28 2001-11-12 The Trustees Of The University Of Pennsylvania Recombinant lentiviral vectors pseudotyped in envelopes containing filovirus binding domains
AU2002348103A1 (en) * 2001-10-26 2003-05-06 Methods for gene transfer using pseudotyped lentiviruses
AU2003237374A1 (en) 2002-06-04 2003-12-19 Centers For Disease Control And Prevention Improved pseudotyped retroviruses
US10561722B2 (en) * 2014-09-03 2020-02-18 Bavarian Nordic A/S Methods and compositions for enhancing immune responses
MY183072A (en) * 2014-09-03 2021-02-10 Bavarian Nordic As Methods and compositions for inducing protective immunity against filovirus infection
WO2018011768A1 (fr) 2016-07-15 2018-01-18 Janssen Vaccines And Prevention B.V. Méthodes et compositions permettant l'induction d'une immunité protectrice contre l'infection par le virus de marburg
EP3606553A1 (fr) 2017-04-06 2020-02-12 Janssen Vaccines & Prevention B.V. Régime de primo-vaccination/rappel par mva-bn et ad26.zebov ou ad26.filo

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8500918D0 (en) * 1985-01-15 1985-02-20 Cancer Res Inst Viral isolates
US5278056A (en) * 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5591624A (en) * 1988-03-21 1997-01-07 Chiron Viagene, Inc. Retroviral packaging cell lines
US5185440A (en) * 1989-06-20 1993-02-09 North Carolina State University cDNA clone coding for Venezuelan equine encephalitis virus and attenuating mutations thereof
WO1991000047A1 (fr) * 1989-06-30 1991-01-10 The Regents Of The University Of California Detection de retrovirus
US5512421A (en) * 1991-02-19 1996-04-30 The Regents Of The University Of California Generation, concentration and efficient transfer of VSV-G pseudotyped retroviral vectors
JPH08504091A (ja) * 1992-09-22 1996-05-07 メディカル・リサーチ・カウンシル 外部表面に非ウィルス性ポリペプチドを提示する組換えウィルス
US5491084A (en) * 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
US5739018A (en) * 1996-08-07 1998-04-14 The Regents Of The University Of California Packaging cell lines for pseudotyped retroviral vectors

Also Published As

Publication number Publication date
WO2000008131A2 (fr) 2000-02-17
AU5465899A (en) 2000-02-28
WO2000008131A3 (fr) 2000-06-02
WO2000008131A9 (fr) 2000-08-03

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