WO2000005389A3 - Production of proteins - Google Patents

Production of proteins Download PDF

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Publication number
WO2000005389A3
WO2000005389A3 PCT/EP1999/004919 EP9904919W WO0005389A3 WO 2000005389 A3 WO2000005389 A3 WO 2000005389A3 EP 9904919 W EP9904919 W EP 9904919W WO 0005389 A3 WO0005389 A3 WO 0005389A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
proteins
production
secretion
hydrophobic stretch
Prior art date
Application number
PCT/EP1999/004919
Other languages
French (fr)
Other versions
WO2000005389A2 (en
Inventor
Leon Gerardus Joseph Frenken
Cees Sagt
Arie J Verkleij
Cornelis Theodorus Verrips
Original Assignee
Unilever Nv
Unilever Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv, Unilever Plc filed Critical Unilever Nv
Priority to AU55049/99A priority Critical patent/AU5504999A/en
Publication of WO2000005389A2 publication Critical patent/WO2000005389A2/en
Publication of WO2000005389A3 publication Critical patent/WO2000005389A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method for producing a heterologous protein in a lower eukaryotic cell, followed by secretion of said protein from said cell, said protein comprising at least one hydrophobic stretch, wherein the protein is glycosylated such as to increase the level of secretion of the protein, without substantially affecting the functionality of the protein and wherein at least one N-glycosylation group is located between the N-terminus of the protein and the first hydrophobic stretch.
PCT/EP1999/004919 1998-07-20 1999-07-08 Production of proteins WO2000005389A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU55049/99A AU5504999A (en) 1998-07-20 1999-07-08 Production of proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98202432.5 1998-07-20
EP98202432 1998-07-20

Publications (2)

Publication Number Publication Date
WO2000005389A2 WO2000005389A2 (en) 2000-02-03
WO2000005389A3 true WO2000005389A3 (en) 2000-06-02

Family

ID=8233953

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004919 WO2000005389A2 (en) 1998-07-20 1999-07-08 Production of proteins

Country Status (2)

Country Link
AU (1) AU5504999A (en)
WO (1) WO2000005389A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399286A (en) * 2016-10-18 2017-02-15 江南大学 Method for promoting Pichia pastoris to produce alkaline pectinase

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA02011911A (en) 2000-06-02 2003-05-27 Novozymes As Cutinase variants.
PL1709073T3 (en) * 2003-11-26 2012-01-31 Max Planck Gesellschaft SUBSTANCE BINDING HUMAN IgG Fc RECEPTOR IIb (FcgammaRIIb)
BR112012018822A2 (en) 2009-12-21 2019-09-24 Danisco Us Inc surfactants that optimize the cleaning of lipase-treated lipase-treated stains
CN109022518A (en) 2011-07-22 2018-12-18 诺维信北美公司 For pre-treating cellulosic material and the method for improving its hydrolysis
EP3031822A1 (en) * 2014-12-08 2016-06-15 Novartis AG Cytomegalovirus antigens
WO2016150845A1 (en) * 2015-03-20 2016-09-29 Ablynx Nv Glycosylated immunoglobulin single variable domains
CN109370430B (en) * 2018-09-20 2020-07-28 陕西师范大学 Aggregation-induced luminescent type ultrathin lysozyme fluorescent nano-film and anti-counterfeiting application thereof
MX2021004477A (en) * 2018-10-17 2021-06-04 Perfect Day Inc Recombinant components and compositions for use in food products.
CN117616120A (en) 2021-06-30 2024-02-27 丹尼斯科美国公司 Variant lipases and uses thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0324274A1 (en) * 1987-12-30 1989-07-19 Chiron Corporation Improved expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences
EP0394951A1 (en) * 1989-04-26 1990-10-31 Takeda Chemical Industries, Ltd. Glycosylated bFGF and production thereof
WO1994014963A1 (en) * 1992-12-18 1994-07-07 Unilever N.V. Modified cutinases, dna, vector and host
WO1996005228A1 (en) * 1994-08-17 1996-02-22 Creative Biomolecules, Inc. METHOD OF PRODUCING SINGLE-CHAIN Fv MOLECULES
EP0704527A2 (en) * 1994-08-05 1996-04-03 PLIVA, farmaceutska, kemijska, prehrambena i kozmeticka industrija dionicko drustvo DNA sequences encoding biosynthetic insulin precursors and process for prepation of insulin

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0324274A1 (en) * 1987-12-30 1989-07-19 Chiron Corporation Improved expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences
EP0394951A1 (en) * 1989-04-26 1990-10-31 Takeda Chemical Industries, Ltd. Glycosylated bFGF and production thereof
WO1994014963A1 (en) * 1992-12-18 1994-07-07 Unilever N.V. Modified cutinases, dna, vector and host
EP0704527A2 (en) * 1994-08-05 1996-04-03 PLIVA, farmaceutska, kemijska, prehrambena i kozmeticka industrija dionicko drustvo DNA sequences encoding biosynthetic insulin precursors and process for prepation of insulin
WO1996005228A1 (en) * 1994-08-17 1996-02-22 Creative Biomolecules, Inc. METHOD OF PRODUCING SINGLE-CHAIN Fv MOLECULES

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BEKKERS A.C.A.P.A. ET AL.: "The use of genetic engineering to obtain efficient production of porcine pancreatic phospholipase A2 by Saccharomyces cerevisiae", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1089, 1991, pages 345 - 351, XP002088381 *
BLOND-ELGUINDI S. ET AL.: "Affinity panning of a library of peptides displayed on bacteriophages reveals the binding specificity of BiP", CELL, vol. 75, 1993, pages 717 - 728, XP002087932 *
CAPLAN S. ET AL.: "Glycosylation and structure of the yeast MF-alpha-1 alpha-factor precursor is important for efficient transport through the secretory pathway", JOURNAL OF BACTERIOLOGY, vol. 173, no. 2, January 1991 (1991-01-01), pages 627 - 635, XP002088382 *
PRAETORIUS-IBBA M. ET AL.: "Homologous recombination partly restores the secretion defect of underglycosylated acid phosphatase in yeast", CURRENT GENETICS, vol. 32, no. 3, 1997, pages 190 - 196, XP002088383 *
SAGT C.M.J. ET AL.: "Impaired secretion of a hydrophobic cutinase by Saccharomyces cerevisiae correlates with an increased association with immunoglobulin heavy-chain binding protein (BiP)", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 1, January 1998 (1998-01-01), pages 316 - 324, XP002088542 *
ZHU H. ET AL.: "Glu-96 of basic fibroblast growth factor is essential for high affinity receptor binding", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 37, 15 September 1995 (1995-09-15), pages 21869 - 21874, XP002073993 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399286A (en) * 2016-10-18 2017-02-15 江南大学 Method for promoting Pichia pastoris to produce alkaline pectinase
CN106399286B (en) * 2016-10-18 2019-12-24 江南大学 Method for promoting pichia pastoris to produce alkaline pectinase

Also Published As

Publication number Publication date
WO2000005389A2 (en) 2000-02-03
AU5504999A (en) 2000-02-14

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