WO2000004192A8 - Methods for detecting and mapping genes, mutations and variant polynucleotide sequences - Google Patents

Methods for detecting and mapping genes, mutations and variant polynucleotide sequences

Info

Publication number
WO2000004192A8
WO2000004192A8 PCT/US1999/016242 US9916242W WO0004192A8 WO 2000004192 A8 WO2000004192 A8 WO 2000004192A8 US 9916242 W US9916242 W US 9916242W WO 0004192 A8 WO0004192 A8 WO 0004192A8
Authority
WO
WIPO (PCT)
Prior art keywords
components
present
formats
probe
signaling
Prior art date
Application number
PCT/US1999/016242
Other languages
French (fr)
Other versions
WO2000004192A1 (en
Inventor
David A Shafer
Original Assignee
Univ Emory
David A Shafer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Emory, David A Shafer filed Critical Univ Emory
Priority to CA002335324A priority Critical patent/CA2335324A1/en
Priority to AU50032/99A priority patent/AU5003299A/en
Priority to EP99934134A priority patent/EP1112378A1/en
Publication of WO2000004192A1 publication Critical patent/WO2000004192A1/en
Publication of WO2000004192A8 publication Critical patent/WO2000004192A8/en
Priority to US12/334,036 priority patent/US20090275029A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention includes a number of related designs for gene probe components, multilinking components and signaling components, all of which are modular in nature and can be used together or in part. These components are generally joined together in composite structures by hybridization of complementary sub-segments, called linkers. The reporters of the present invention are also designed to be conjoined into arrays that can provide amplified signaling. The multilinking components of the present invention may be interposed between the probe and the reporter units and provide for the binding of multiple reporters. These probe and signaling methods also include means to achieve mixed-color labeling that is specific to each target. The present invention is useful for detecting target sequences in a wide variety of formats including, but not limited to, membrane formats, in situ formats, and on various solid substrate chip formats.
PCT/US1999/016242 1998-07-17 1999-07-16 Methods for detecting and mapping genes, mutations and variant polynucleotide sequences WO2000004192A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002335324A CA2335324A1 (en) 1998-07-17 1999-07-16 Methods for detecting and mapping genes, mutations and variant polynucleotide sequences
AU50032/99A AU5003299A (en) 1998-07-17 1999-07-16 Methods for detecting and mapping genes, mutations and variant polynucleotide sequences
EP99934134A EP1112378A1 (en) 1998-07-17 1999-07-16 Methods for detecting and mapping genes, mutations and variant polynucleotide sequences
US12/334,036 US20090275029A1 (en) 1999-07-16 2008-12-12 Systems and Methods to Quantify and Amplify Both Signaling and Probes for CDNA Chips and Gene Expression Microarrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9321998P 1998-07-17 1998-07-17
US60/093,219 1998-07-17

Related Child Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2001/007508 Continuation-In-Part WO2001066802A1 (en) 1999-07-16 2001-03-09 SYSTEMS AND METHODS TO QUANTIFY AND AMPLIFY BOTH SIGNALING AND PROBES FOR cDNA CHIPS AND GENES EXPRESSION MICROARRAYS
US10/380,596 Continuation-In-Part US7482443B2 (en) 1999-07-16 2001-03-09 Systems and methods to quantify and amplify both signaling probes for cDNA chips and genes expression microarrays

Publications (2)

Publication Number Publication Date
WO2000004192A1 WO2000004192A1 (en) 2000-01-27
WO2000004192A8 true WO2000004192A8 (en) 2000-07-13

Family

ID=22237801

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/016242 WO2000004192A1 (en) 1998-07-17 1999-07-16 Methods for detecting and mapping genes, mutations and variant polynucleotide sequences

Country Status (4)

Country Link
EP (1) EP1112378A1 (en)
AU (1) AU5003299A (en)
CA (1) CA2335324A1 (en)
WO (1) WO2000004192A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9169321B2 (en) 2007-08-22 2015-10-27 The Regents Of The University Of California Activatable binding polypeptides and methods of identification and use thereof

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066802A1 (en) * 2000-03-09 2001-09-13 Genetag Technology, Inc. SYSTEMS AND METHODS TO QUANTIFY AND AMPLIFY BOTH SIGNALING AND PROBES FOR cDNA CHIPS AND GENES EXPRESSION MICROARRAYS
US8673570B2 (en) * 2000-03-09 2014-03-18 Genetag Technology, Inc. System and methods to quantify and amplify both signaling and probes for cDNA chips and gene expression microarrays
US6893822B2 (en) * 2001-07-19 2005-05-17 Nanogen Recognomics Gmbh Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
US7632639B2 (en) 2003-02-21 2009-12-15 Eisai R&D Management Co., Ltd. Signal amplification method for detecting mutated gene
WO2005061733A1 (en) * 2003-11-20 2005-07-07 Singulex, Inc. Preparation of defined highly labeled probes
GB0404187D0 (en) * 2004-02-25 2004-03-31 Biotransformations Ltd Binding agents
JP2005304489A (en) * 2004-03-24 2005-11-04 Sysmex Corp Probe set for detection of target substance and detection method using the same
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
US8685899B2 (en) 2007-02-14 2014-04-01 Genisphere Inc. Methods, reagents and kits for detection of nucleic acid molecules
EP2385955B1 (en) 2009-01-12 2020-08-12 CytomX Therapeutics, Inc. Modified antibody compositions, methods of making and using thereof
JP5861223B2 (en) 2009-02-23 2016-02-16 サイトムエックス セラピューティクス, インク.CytomX Therapeutics, Inc. Proprotein and its use
EP2949759B1 (en) 2014-05-27 2017-07-26 Université Paris Sud 11 Multiplexed homogeneous oligonucleotide detection
CN108603229A (en) * 2016-01-22 2018-09-28 格瑞尔公司 method and system for high-fidelity sequencing
CA3029178C (en) * 2016-06-30 2022-07-19 Biois Co.,Ltd Double-stranded nucleic acid signal probe and method for detecting target molecule using same
US12049665B2 (en) 2018-06-12 2024-07-30 Accuragen Holdings Limited Methods and compositions for forming ligation products
WO2020113569A1 (en) * 2018-12-07 2020-06-11 深圳华大生命科学研究院 Method for sequencing long fragment nucleic acid

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5359100A (en) * 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
HUT66828A (en) * 1991-03-27 1995-01-30 Res Corp Technologies Inc Single-stranded circular oligonucleotides
WO1995001365A1 (en) * 1993-07-02 1995-01-12 Lynx Therapeutics, Inc. Synthesis of branched nucleic acids
FR2710075B1 (en) * 1993-09-15 1995-10-27 Bio Merieux Reagent and method for the detection of a nucleotide sequence with signal amplification.
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5912124A (en) * 1996-06-14 1999-06-15 Sarnoff Corporation Padlock probe detection
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9169321B2 (en) 2007-08-22 2015-10-27 The Regents Of The University Of California Activatable binding polypeptides and methods of identification and use thereof

Also Published As

Publication number Publication date
EP1112378A1 (en) 2001-07-04
AU5003299A (en) 2000-02-07
CA2335324A1 (en) 2000-01-27
WO2000004192A1 (en) 2000-01-27

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