WO2000002544A2

WO2000002544A2 - Use of piperine for treating skin pigmentation disorders

Info

Publication number: WO2000002544A2
Application number: PCT/GB1999/002256
Authority: WO
Inventors: Amala Raman; Zhixiu Lin
Original assignee: Btg International Limited
Priority date: 1998-07-13
Filing date: 1999-07-13
Publication date: 2000-01-20
Also published as: ATE249220T1; HK1033264A1; AU757053B2; CA2735844A1; JP2002520275A; CN1330545A; WO2000002544A3; ZA200004260B; EP1094813B1; CA2337205C; CA2337205A1; US6346539B1; DE69911210T2; ES2207252T3; AU4920999A; DE69911210D1; CA2735844C; EP1094813A2; KR20010071872A; CN1210029C

Abstract

The present invention provides piperine and analogues or derivatives thereof for re-pigmenting post traumatised de-pigmented skin and for the treatment of skin conditions such as vitiligo. The piperine and analogues or derivatives thereof may also be used to cosmetically promote or enhance the natural colouration of the skin.

Description

TREATMENT OF SKIN DISORDERS This invention relates to the treatment of skin disorders and more particularly to those disorders requiring stimulation of melanocyte proliferation. The invention is of especial application to the treatment of vitiligo. Vitiligo is a common skin pigment disorder characterised by the development of patchy depigmented lesions. Current treatments which include the use of photosensitisers (eg psoralens) with UVA radiation (PUVA), corticosteroids or skin grafting have low success rates and are generally accompanied by unpleasant side effects. Vitiligo has a highly detrimental impact on the emotional well-being of the sufferer, the disfiguring effects of the disease being compounded by the absence of a suitable treatment. Although vitiligo patches are not believed to contain melanocytes (pigment producing cells), a reservoir exists in hair follicles in vitiliginous skin. Thus activation of hair follicular melanocytes is a crucial process in the repigmentation of vitiliginous skin.

Certain plant remedies, usually administered as mixtures of herbs or extracts, particularly those used in traditional Chinese medicine and Indian Ayurvedic medicine, have been employed for the treatment of vitiligo for a long time and in many cases have given positive results in small scale studies. Herbs such as Psoralea corylifolia L. and Vernonia anthelmintica Willd. (=Centratherum anthelminticum Kuntze) are well known for their use in this disease. Psoralens, which are employed in the modern PUVA and khellin in KUVA therapy were originally derived from plant sources (Psoralea corylifolia L and Ammi visnaga respectively) used in traditional remedies for vitiligo. However these therapies rely on the use of UV irradiation for their efficacy, which is associated with the aetiology of skin cancer.

The fruit of black pepper (Piper nigrum L.) and long pepper (Piper longum L.) are both important medicinal herbs in Ayurvedic and Unani (traditional Indian) medicine systems, in which remedies generally consist of mixtures of herbs. A wide range of the medicinal uses of black pepper have been documented by Kirtikar and Basu (Indiam Medicinal Plants, 2^nd Edition, Vol. 3, (1935) pages 2128 - 2135), including its use in the treatment of leucoderma. Black pepper has also been implicated as a possible adjunct to Vernonia anthelmintica in the treatment of leucoderma (Indian Medicinal Journal, Vol. 1 , 3^rd Edition, (1982) 1267 - 1270). These two herbs are employed as a constituent in many traditional herbal preparations for a variety of uses, including gastro-intestinal and skin ailments. Compositions comprising black pepper, ginger and pipali have been used in the treatment of vitiligo (Ancient Science of Life, Vol. IX. No. 4 (1990) 202 - 206); however, the specific therapeutic action of black pepper in this orally administered composition has not been established.

There is, therefore, a need for further compounds and compositions, which are able to stimulate the proliferation of melanocytes. Preferably the compounds and compositions of the invention are able to stimulate the proliferation of melanocytes without significant adverse side effects. The present invention addresses that need. The present inventors have screened 30 herbal extracts, which have been used traditionally in the treatment of vitiligo using a cell culture system to evaluate whether they have the ability to directly stimulate the proliferation of melanocytes. It has been surprisingly found that components of Piper nigrum L. fruit possess melanocyte proliferant activity. In particular, it has been found that the component piperine is able to significantly stimulate the production of melanocytes.

Piperine has also been reported to occur in other Piper species ie. P. acutisleginum, album, argyrophylum, attenuatum, aurantiacum, betle, callosum, chaba, cubeba, guineense, hancei, khasiana, longum, macropodum, nepalense, novae hollandiae, peepuloides, retrofractum, sylvaticum. Pharmaceutical compositions containing piperine have been used in the treatment of tuberculosis and leprosy (EP 0 650 728). It has also been suggested that piperine is able to enhance the bioavailability of the other constituents of a pharmaceutical composition (WO 96/25939).

A first aspect of the invention provides the use of piperine or an active analogue or derivative thereof for the stimulation of melanocyte proliferation. The piperine may be used in isolated form, but is more suitably used in combination with a carrier or excipient and optionally one or more further active ingredients. It may also be used in the form of a plant extract, derivable from Piper nigrum, for example.

It will be appreciated that stimulation of melanocyte proliferation greatly facilitates the re-pigmentation of de-pigmented skin. De-pigmentation of the skin may arise, for example, from scar tissue formed as a result of a skin trauma such as burn or other skin lesion or may be due to skin conditions such as vitiligo. Compositions comprising piperine or an active analogue or derivative thereof may therefore be used to re-pigment post traumatised de-pigmented skin as well as conditions such as vitiligo.

A second aspect of the invention provides the use of piperine or an active analogue or derivative thereof in the preparation of a medicament for the stimulation of melanocytes. The medicament may be in the form of a solid powder; a paste, ointment or cream; a tablet or capsule; or a solution. The piperine or active derivative or analogue thereof may be administered by oral, topical, intravenous or subcutaneous (intramuscular) routes. It is preferred that the medicament is applied topically to the de- pigmented area of the skin. In a first embodiment of the second aspect of the invention there is provided the use of piperine or an active analogue or derivative thereof in the preparation of a medicament for re-pigmententing post traumatised de-pigmented skin. By the term "post traumatised de-pigmented skin" it is to be understood to mean the skin formed during the healing process that occurs after a skin trauma such as a burn or a lesion. In a second preferred embodiment of the second aspect of the invention there is provided the use of piperine or an active analogue or derivative thereof in the preparation of a medicament for the treatment of vitiligo. Although the compositions may be administered orally, topical administration is preferred.

The invention also provides a method of re-pigmenting de-pigmented skin or treating vitiligo comprising the administration to a patient requiring therapy of a pharmaceutically acceptable amount of piperine or an active derivative or analogue thereof.

Stimulation of melanocyte proliferation may also be used to enhance or promote the natural colouring of the skin and compositions comprising piperine or an active analogue or derivative thereof can be used as tanning agents. The enhancement of natural skin colour may be used for prophylactic or cosmetic purposes. A third embodiment of the second aspect of the invention provides the use of piperine or an active analogue or derivative thereof in the preparation of a composition for enhancing or promoting the colour of the skin. In addition a third aspect of the invention provides a method for cosmetically enhancing or promoting the colouring of the skin comprising the administration to a

j - person desiring such an effect of a cosmetically effective amount of piperine or an active analogue or derivative thereof. Administration may be by oral or topical routes.

It will be appreciated that certain of the active analogues or derivatives of piperine have not hereto before been used for therapeutic purposes and a further aspect of the invention provides an active analogue or derivative of piperine for use in the stimulation of melanocyte proliferation. The invention also provides an active analogue or derivative of piperine for use in therapy. Therapeutic uses include the re-pigmentation of de- pigmented skin as well as the promotion or enhancement of skin colouration.

Piperine has also been found to inhibit the proliferation of melanoma cells. Piperine and its analogues or derivatives may also be used in the treatment of skin cancer.

A fourth aspect of the invention provides a method of treating or preventing skin cancer in a human or non-human patient comprising the administration to said patient of a therapeutical ly effective amount of piperine or an active analogue or derivative thereof.

A further aspect of the invention provides the use of piperine or an active analogue or derivative thereof in the preparation of a medicament for the treatment of skin cancer.

The piperine or active analogue or derivative thereof may be administered by oral or topical routes. Suitable dosage forms may be any of those discussed herein above.

The structure of piperine and derivatives and analogues thereof is given below.

Variation in stereochemistry at double bonds Structures for comparison and in extent of conjugation in chain

1 (E, E) - Piperine

2 (Z, Z) - Chavicinc

3 (Z, E) - Isopiperine

4 (E, Z) - Isochavicine

5 3,4-dιhydropιperme- Piperanine

6 1 ,2,3,4-Tetrahydropiperinc

Variation in separation of rings (conjugated) Structures for comparison (all E)

O 7 n = 1 - Ilepcimide 1 n = 2 - Piperine cσ 8 n = 3 - Piperettine

Alterations to nitrogen substituent Structures for comparison (all E,E)

0 R=

I pipendinyl Piperine 9 pyrrohdinyl Trichostachine lO isobutyiamtno ■ Piperlonguminine

I I methoxy Despiperidylmethoxypiperine

Alterations to the phenyl substituent Structures for comparison (all E,E)

e

The naturally occurring compounds (including piperine) may be extracted from suitable plant sources or synthesised using methods known to a skilled person (see, for example, Chapman and Hall, Combined Chemical Dictionary on CD-Rom, Release 1 :1 (1997) and The Merck Index (1983), 10th edition. Publ. Merck and Co, Rahway, USA. PP. 1077-1078 (except compounds 2 and 3)). All compounds except 6, 11, 13, 15 and 16 occur in P. nigrum or other Piper species (10 and 12).

Compounds 2 and 3 are prepared by isolation from P. nigrum using methods known to a skilled person (see, for example, Cleyn R De and Verzele M (1975). Constituents of Peppers. Part VII. Spectroscopic Structure Elucidation of Piperine and its Isomers. Bulletin de la Societe Chimique Belgique, 84, 435-438).

Compound 6 is prepared by hydrogenation of piperine over Adams catalyst using known methods (see for example Banerji A, Bandyopadhyay D, Sarkar M et al (1985). Structural and synthetic studies on the retrofractamides - amide constituents of Piper retrofractum, Phytochemistry, 24, 279-284).

Compound 1 1 is prepared by methanolysis of piperine using sodium methoxide. Compounds 13 and 15 are prepared from 3-hydroxy-4-methoxybenzaldehyde and benzaldehyde respectively as starting materials using methods analogous to those used for the preparation of piperine.

Compound 16 is prepared from the reaction between piperidine and E,E- hexadienoyl chloride. The active compounds may be formulated for topical use in the form of creams, soft paraffin or lotions. Aqueous cream BP or Yellow Soft Paraffin BP may suitably contain the active at 0.03-3.0 mg % w/w or an equivalent amount of plant extract. A suitable lotion is typically prepared from 20% glycerol and 80% ethanol in purified water and contains 0.03-3.0 mg % w/w of the active material. These topical formulations may also contain penetration enhancers such as oleic acid, propylene glycol, ethanol, urea, lauric diethanolamide or azone, dimethyl sulphoxide, decylmethyl sulphoxide, or pyrrolidone derivatives. Liposomal delivery systems may also be used.

Compositions for oral formulation include tablets or capsules containing 1.5-150 mg active or equivalent amount of plant extract. Figures and Examples

The invention will now be described with reference to the following non-limiting examples, with reference to the accompanying tables and drawings in which: Figure 1 : Melan-a growth curves under different medium compositions. Each point represents the mean and standard deviation of 6 wells. Figure 2: Melan-a growth curves of different initial plating densities detected with SRB assay. Medium was supplemented with 20 nM TPA. On day 4 the medium in the remaining plates was replaced. Each point shows mean and SD of 6 replicates. Figure 3: Effect of P nigrum extract on the growth of melan-a cells. Culture was maintained for 8 days. Medium and extract were replaced with fresh ones on day 4. Each point designates mean and SD of 6 wells except 12 replicates for cells only. Figure 4: Effects of P nigrum extract and TPA on the proliferation of melan-a cell line.

Each point shows mean and SD of 6 replicates except 12 wells used for cells only. Figure 5: Effects of piperine and TPA on the growth of melan-a cells in the presence of

RO-31-8220. n = 6 for piperine and TPA treated wells, whereas n = 12 for RO- 31-8220 alone.

Figure 6: Effects of piperine and TPA on the growth of human melanoblasts in the presence of ET3. *P < 0.05 when compared to vehicle control (One way Anova, followed by Dunnett's t-test) Figure 7: Effects of piperine on the growth of human melanocytes in the presence of ET1. *P < 0.05 when compared to ET1 InM treatment (One way Anova, followed by

Dunnett's t-test)

Variations on these examples will be apparent to a person skilled in the art.

Examples

Plant samples and preparation of extracts

Piper nigrum L. fruit (black pepper, Piperaceae), originally from India, was purchased from the Food Centre, 70 Turnpike Lane, London N8, UK. The rest of the herbs were either supplied by East- West Herbs, Kingham, Oxon, UK or by Cipla Ltd, Mumbai, India.

For the preliminary screening programme, the powdered dry herb (10 g) was heated to boiling in distilled water (100 ml) and allowed to boil for 10 min, using a hot plate as heat source. The plant material was filtered off under vacuum through filter paper (Whatman), and the filtrate freeze-dried.

Cell culture experiments

Microplate culture and sulforhodamine B (SRB) assay

Cells of mouse melan-a cell line (passage number 18-24), a first known line of non-tumorigenic pigmented mouse melanocytes were maintained in a flask (Costar, Cambridge, MA, USA) using RPMI 1640 (ICN, Costa, Mesa, CA, USA) as a basic medium. For microplate proliferation assays, subconfluent melan-a cultures were trypsinized (0.25% trypsin at 37°C for 5-10 min) and inoculated with a repeater-pipettor (Finn pipette, Labsystems, Finland) into 96-well microtiter plates (Costar, Cambridge, MA, USA). The plates were incubated at 37°C in a 10% CO₂, 90% air humidified atmosphere for the stated length of time. At the end of the incubation, an SRB assay was performed. Briefly, cells attached to the bottom of the plate were fixed by addition of cold trichloroacetic acid (TCA, 4°C, Aldrich, Dorset, UK) on the top of the growth medium (final TCA 20% w/v). The plate was placed at 4°C for 1 hour before being gently washed five times with tap water. It was allowed to dry in air, or aided with a hair dryer to speed up the drying process, then 50 μl of 4% w/v SRB dissolved in 1% acetic acid in water was added to each well for 30 min. At the end of the staining period, unbound SRB was removed by washing 4 times with 1% acetic acid. The plate was air dried again, and 150 μl of 10 mM aqueous Tris base (Sigma-Aldrich Co. Ltd, Irvine, UK) was added into each well to solubilize the cell-bound dye. The plate was shaken for 15 min on a gyratory shaker followed by reading the optical density (OD) at 550 nm in a microplate spectrophotometer (Anthos Labtec HT3, version 1.06)

Example 1

Optimisation of incubation conditions - FBS concentration and cell seeding density

Prior to testing the herbal extracts, optimal culture conditions were established. The variable factors regarding incubation conditions include foetal bovine serum (FBS) concentration, initial cell seeding density and incubation period. To determine optimum FBS concentration, 1 , 2, and 5% FBS were used to culture the melan-a cell line, the growth pattern with each concentration of FBS was monitored by SRB assay. For the determination of optimum cell seeding density, a series of initial seeding density of 0.15 to 1.2 x 10⁴ cell per well of melan-a cells were plated into 96-well plates with 5% FBS and 20 nM tetradecanoyl phorbol acetate (TPA) supplemented growth medium. The growth pattern was monitored with SRB assay at daily intervals. The culture was extended to 8 days; on day 4, the medium in the remaining plates was replaced.

Results

The effect of FBS concentrations on melan-a growth The optimal condition for the negative experimental control, is that cells neither grow too fast nor decline dramatically. Rapid growth might mask any subtle stimulatory effect brought about by the herbal extracts, whereas a dramatic decline in cell numbers indicates unfavourable culture conditions for cell survival, which could lead to cell damage. Figure 1 shows the growth curves of melan-a cell line at three different concentrations of FBS. Neither 1% nor 2% FBS supplemented medium was able to maintain cell survival; cell numbers declined significantly in 4 days of culture. However, 5% FBS was capable of keeping melan-a cell line alive with only a small increase in cell numbers observed over 4 days. TPA (20 nM) was able to cause further proliferation in the presence of 5% FBS indicating that cells were capable of responding to mitogenic stimuli at 5% FBS. Morphological observations under a microscope revealed that with 1% and 2% FBS supplemented medium, cell bodies were round, lightly pigmented with few dendritic processes and the culture displayed an ageing growth pattern. However in 5% FBS, cells possessed more melanosomes and some short dendrites without an ageing appearance. Therefore 5% FBS was used throughout in the herbal screening experiments.

Growth curves of melan-a cell line with various seeding densities

In Figure 2, growth curves over 8 days with different initial cell numbers were plotted to elucidate the melan-a cell line's growth pattern in 96-well plates in the presence of 5% FBS and 20 nM TPA. The optimal initial plating density together with proper harvesting time was determined. All of the initial plating number of cells showed a net growth in the presence of TPA and 5% FBS supplemented medium, although the higher plating density of 1.2 x 10⁴ cells/well depleted the growth medium on day 3 of culture and the cells ceased to grow until the medium was replaced. With the lower plating densities (2 - 4 x 10³ cells/well) the SRB assay OD readings remained relatively low after 8 days' culture. The initial plating density of 6 x 10³ cells/well exhibited exponential growth, and after 4 days of culture, the OD reading increased to a value of about 0.4. Since the higher OD values are associated with greater precision and accuracy, it was determined that the initial inoculation of 6 x 10³ cells/well was the optimum density for the herbal test experiment. For the simplicity of the experiment, harvesting time was day 4 since the cells at this stage was not confluent and after 4 days, growth medium tended to become depleted and replacement was necessary for the further growth.

Example 2 Preliminary herbal screening experiments

Melan-a cells were seeded at a density of 6 x 10³/100μl/well in standard medium supplemented with 0 nM TPA and 5% FBS. After 4 hours of incubation, herbal extracts, which were reconstituted in growth medium and sterilised by filtration (pore size 0.2 μ ), of different concentrations was added into each well. Final concentrations of plant extract were 0 (negative control), 10, 100 and 1000 μg dry extract per ml. 6 replicate wells were used for each concentration tested. The negative control (12 wells), positive control (20 nM TPA, 6 wells), and test wells were all in the same 96-well plate. The culture was terminated after 4 days and SRB assay performed according to the methods given above.

Results

The effect of 30 herbal extracts on the proliferation of melan-a cell line

Table 1 shows the results of the preliminary screening of 30 aqueous herbal extracts on the proliferation of melan-a cell line. Crude extracts of Astragalus membranaceous (Fisch.) Bunge, unripe Citrus reticulata Blanco, Dictamnus dasycarpus Turcz., Ophiopogon japonicus (Thunb.) Kergawe, Piper nigrum L., Poria cocos (Schw.) Wolf and Tribulus terestris L. were observed to stimulate melanocyte proliferation, sometimes even at the lowest dose level of 10 μg/ml. Other extracts either had no significant effect or were cytotoxic. Among these positive responses, that of Piper nigrum L. extract at 0.01 and 0.1 mg/ml was the most pronounced. Piper nigrum extract at these two concentrations not only strikingly enhanced cell growth, but this extract also altered the cell morphology. In the presence of Piper nigrum extract, the cellular bodies were smaller, with more and longer bipolar or polydendritic processes, an effect similar to that observed with TPA.

Example 3

Repeats of the tests on Piper nigrum extract on the melan-a cells A newly prepared Piper nigrum fruit extract was tested on a new batch of melan-a cell line with the culture in microplates extended to 8 days. The effects of Piper nigrum extract on the growth of melan-a cell line were evaluated by SRB assay.

Results

Repeats of the tests of Piper nigrum extract on melan-a cells

In the light of the positive results from the preliminary experiment, further investigations on Piper nigrum extract were carried out. Figure 3 shows that the result of the significant proliferant effect brought about by the Piper nigrum extract was even more marked on the extension of the incubation period to 8 days of culture, the growth was 272% of the control (cells only). Microscopically, the morphology of the cells was altered as those seen in the preliminary experiments.

Example 4 Confirmation of the proliferant effect of Piper nigrum by haemocytometer counting

Melan-a cells were plated in petri dishes (035 mm, Nunclon, Denmark) with a plating density of 2 x 10⁴/ml and Piper nigrum extract at concentrations of 0.01 and 0.1 mg/ml. A negative control (cells in medium only) and positive TPA (20 nM) control were also set up. After 4 days the cells in each dish were harvested and counted with haemocytometer.

Results

Confirmation of the proliferant effect of Piper nigrum by haemocytometer counting

SRB assay indirectly estimates cell number through protein staining and spectrophotometric measurement. To confirm if Piper nigrum extract stimulates melan-a cell proliferation, a direct cell counting with haemocytometer method was employed.

Table 2 shows the cell numbers in the presence of Piper nigrum extract and 20 nM TPA.

Cell number under the influence of Piper nigrum extract at 0.01 and 0.1 mg/ml were increased significantly compared to control, but less than that with 20 nM TPA. This result is consistent with the finding in 96-well microplate SRB assay. Example 5

Effect of Piperine on the growth of melan-a cell line

Piperine (Sigma-Aldrich Co. Ltd, Irvine, UK) was dissolved in MeOH, sterilised by filtration through a membrane (pore size 0.2 μm) and diluted with standard growth medium. The final concentrations in culture were 0.1 and 1 μM. A separate experiment

(data not shown) showed that the concentration of MeOH present in these experiments was not toxic or proliferant to the cells.

Results The effect of piperine on the proliferation of melan-a cell line

The effect of this compound on melan-a cell line is shown in Figure 4. Piperine at the two concentrations tested significantly stimulated melan-a proliferation. This compound brought about morphologic changes to melan-a cells, with smaller cell bodies, more and longer cellular dendrites, resembling those alterations induced by Piper nigrum extract and TPA. This indicates that piperine is an active principle responsible for the observed proliferant effect of Piper nigrum.

Example 6

Test of piperine on different cell types to determine its specificity In order to determine the specificity of piperine, a panel of different cell types were employed to facilitate this investigation. These included melan-a, melan-c, SVK14, CSM, XB2, SCI, B16F10, IM9, CACO2, Swiss 3T3 cell lines and normal human lymphocytes. TPA (20 nM) was also tested on these cells. Table 3 shows the biological origin of the cells and an outline of the cell culture protocols.

Results

The effects of piperine and TPA on the growth of a panel of cell types.

From Table 4, it can be seen that piperine has a highly selective effect on the growth of a panel of cell types, since it only stimulates the mouse melanocytes (melan-a, melan-c), human melanoblasts (FM21E), human foetal melanocytes (FM 2 IE) and the mouse fibroblast SCI cell lines at the concentration tested. The SCI cell line may have a particular sensitivity to TPA due to the way in which it has been derived, i.e. it has been cultured in the presence of TPA. However, piperine has either no effect or a cytotoxic effect on other cells. This result implies that piperine may have desirable specificity index for the proliferation of melanocytes in culture and is not a general mitogen. In our experimental system, TPA, a well known PKC activator and a tumour promoting agent, had similar effects to piperine on all cell types tested, except that TPA strikingly stimulated human lymphocyte and 3T3 fibroblast proliferation whereas piperine obviously lacked such an activity. Piperine seems to be a less potent stimulant than TPA.

Example 7

Mode of Action: effect of RO-31-8220 on the growth of melan-a cells with piperine and TPA

Melan-a cell line cultured with piperine 1 μM and TPA 20 nM separately was set up in a 96-well plate, 1 μl of different concentrations of RO-31-8220 (Calbiochem- Novabiochem) in DMSO was introduced with a micro-syringe into the wells to make up the final RO-31-8220 concentrations of 0 (control), 0.1, 1, 5, 10, 100 nM, with final DMSO concentrations smaller than 0.01% v/v, at which the DMSO showed neither toxic nor proliferant effect to the cells in a separate experiment (data not shown). 6 replicate wells were used for each concentration. The culture was incubated for 4 days before it was terminated and processed with SRB assay to evaluate the growth of melan-a cells.

Results

Mode of Action: effect of RO-31 -8220 on the growth of melan-a cells with piperine and

TPA Figure 5 shows the effect of RO-31-8220 on the survival and growth of melan-a cell line in the presence or absence of piperine and TPA. RO-31-8220 alone did not have significant cytotoxic effect to the cells at the concentrations up to 100 nM. However, the proliferant effects of piperine, and TPA (as indicated by the Y axis values) on melan-a cells were effectively inhibited by the presence of RO-31-8220 at the concentrations of 0.1 - 100 nM. It thus appears that piperine and TPA exert their proliferant effects through the activation of PKC cell signalling pathway. The selectivity of piperine on the growth of a panel of cell types has also been tested. It was found that piperine possessed a fairly high specificity and selectivity towards melanocytes, since it significantly stimulated the growth of melan-a, melan-c and FM21E melanoblasts and FM21E melanocytes in culture, whereas it did not stimulate all other cells apart from a TPA-sensitive fibroblast cell line. Piperine was observed to have inhibitory effects on B16 mouse melanoma cell line which is syngeneic with melan-a cells. Thus piperine may be a specific stimulant for the proliferation of melanocytes in vitiliginous skin without the risk of stimulating melanoma cells.

Example 9

Experiments on human melanoblasts in culture

Human melanoblasts in culture in this experiment were established from human foetal skin. Subconfluent melanoblasts maintained in MCDB 153 medium supplemented with 10% FBS, 10 ng/ml stem cell factor (SCF) and 1 nM endothelin 3 were subcultured and inoculated into 96-well microplate with 6 x 10³ cells/1 OOμl/well. After incubation in the 10% CO , humidified atmosphere, at 37°C for 3 - 4 hours to allow the attachment of the cells on the plate, piperine dissolved in MeOH and water was added into the wells. The final concentrations of piperine were 1, 5, 10, 100 μM, with TPA (20 nM) as positive control. Six replicates were used in each group of treatment, with 12 wells used for vehicle control. The incubation was conducted for 5 days before cells were harvested by fixing with cold trichloroacetic acid (TCA, at 4°C, final concentration 20% v/v), and evaluated for cell number using an SRB assay. One way ANOVA and Dunnett's t-test was employed to test the significance of any differences between treatment groups and vehicle control. Growth in the presence of piperine and TPA was expressed as % of control incubations containing no piperine or TPA. The experiments were repeated using melanoblasts from 3 different donors.

Results

Figure 6 shows the effect of piperine on the growth of human melanoblasts in vitro. Piperine at the concentrations of 1 , 10, 100 μM was found to cause significant stimulation to human melanoblasts in a dose response manner, with 34% more cell yield compared to vehicle control when the culture was exposed to 100 μM piperine in culture for 5 days. TPA, a well-known melanocytic growth-stimulating agent, was also able to cause significant cell growth at tested concentrations, with over 50% of more cell yield observed when the culture was exposed to 20 nM for 5 days. In the other repeated experiments, piperine was consistently observed to induce significant cell growth at the concentrations ranging from 5 - 100 μM; these stimulatory effects were generally less than that of TPA. Morphologically, in the presence of piperine, melanoblasts appeared to be more dendritic and the cell bodies were flatter and smaller.

Example 10

Experiments on human melanocytes in culture

Human melanocytes used in this experiment were derived from induced differentiation of human foetal melanoblasts. The key character of human melanocytes that is different from its precursor melanoblasts is their ability to synthesise melanin. Melanin is a valid marker for melanocytes. The cell pellet of human melanocytes exhibits a characteristic brown to black colour, whereas human melanoblasts cannot produce melanin thus devoid of brown or black colour in the cell pellet.

Two protocols were employed for the experiments on human melanocytes in culture. The first employed 24-well plates and evaluated cell number with SRB assay. The second employed petri dishes and cell number was counted with a haemocytometer chamber. For the first protocol, subconfluent human melanocytes maintained in a 0100 mm petri dish were subcultured into two 24-well plates (Falcon) using basic culture medium of RPMI 1640 supplemented with FBS (10%), bFGF (100 pM), CT (1 nM) and endothelin 1 (1 nM). The initial plating density was 20,000 cells/cm² (38,200 cells/well) with each well containing 1000 μl medium. After incubation in a 10% CO₂, humidified atmosphere, at 37°C for 2 - 3 hours to allow the attachment of the cells, piperine in 500 μl medium was added into wells to made up final concentrations of 0, 1, 5, 10 and 100 μM. Cells only in the medium with above supplement lacking of endothelin 1 were also set up as negative control. Six replicates were used in each group of treatment, and culture was incubated for 5 days before the cells were harvested by fixing with cold TCA (final concentration 20%) and processed with SRB assay. The solubilized SRB dye solution was transferred to a 96-well plate for optical density reading.

For the second protocol, subconfluent human melanocytes were subcultured in a 060 mm petri dishes (28 cm², Falcon) with RPMI 1640 basic medium supplemented with FBS (10%), CT (1 nM), bFGF (100 pM) and endothelin 1 (1 nM). The initial plating density was 10,000 cells/cm , with 5 ml medium per dish. Cells were incubated for 2 - 3 hours in 10% CO₂, humidified atmosphere, at 37°C, followed by addition of piperine solution in to the dishes, making the final concentrations of 0, 1 , 5, 10 and 100 μM. Cells in the above supplemented medium lacking endothelin 1 were also set up as a negative control. Three dishes were used for each treatment and the culture was maintained for 5 days before cells were harvested with trypsinisation and counted with a haemocytometer chamber. For melanin production experiment, the harvested cells were centrifuged and pelleted. After carefully removing the medium, NaOH (1 M) was used to solubilized the cell pellets and optical density read at 475 nm in a Perkin-Elmer UV spectrophotometer (model UV/VIS Lambda 2). The melanin content was calculated by using a regression equation y = 0.005 + 0.005x corresponding to the calibration curve for synthetic melanin.

Results Figure 7 delineates the effects of piperine on the growth of human melanocytes cultured in 24-well plate. Piperine at the concentrations of 5 and 10 μM markedly stimulates the growth of these pigmented cells, with 36% more cells yielded when the culture was under the influence of 10 μM piperine for 5 days. However, at 100 μM, piperine exerted inhibitory effect on the growth of these cells. In addition, in the presence of 1 nM endothelin 1, TPA at 20 nM was not able to stimulate cell growth in our culture system, a result that is of great difference with that observed in human melanoblasts.

Table 5 shows the effects of piperine on the growth of human melanocytes cultured in petri dishes. It is conspicuous that in the presence of ETl (InM), piperine at the concentrations of 5 and 10 μM significantly stimulated the growth of human melanocytes, with cell number over twice as many as that of ETl (InM) control when this melanocyte cell type was exposed to 5 μM piperine for 5 days. This result was consistent with that obtained from the 24-well plate experiments, and it served to confirm that the stimulatory effects observed by SRB assay were indeed due to increased cell number rather than augmentation of protein production alone.

TABLES

Table 1. Preliminary screening of 30 herbal aqueous extracts on the proliferation of melan-a cell line detected with SRB assay after days culture.

Names of herbs Plant part Cell number (% of control) after 4 days incubation when grown in the presence of extract at:

1 mg ml^" 0.1 mg ml^" 0.01 mg ml^"

Plants with a significant stimulatory effect

CO Astragalus membranaceous (Fisch.) Bunge root 163.2* 123.6* 105.6 el a Citrus reticulata Blanco (Qing Pi - unripe) peel 16.0 138.5* 127.6*

H H Dictamnus dasycarpus Turcz. root bark 105.0 159.4* 98.0 a

H Ophiopogon japonicus (Thunb.) Kergawe root 127.8* 126.5* 108.4 Λ oo Piper nigrum L. fruit 11.5 215.4* 151.3*

Poria cocos (Schw.) Wolf (fungus) sclerotium 79.0 134.6* 128.8* Tribulus terrestris L. fruit 80.7 136.1 * 142.2* re t Plants with no significant stimulatory effect Angelica dahurica (Fisch.) Benth. & Hook. root 50.4 1 18.3 107.0 Chaenomeles lagenaria (Loisel.) Koldz. fruit 57.1 74.5 99.0 Citrus reticulata Blanco (Chen Pi - ripe) peel 34.6 101.1 81.1 Corydalis bulbosa D.C. root 91.8 101.2 92.9 Curcuma longa L. root 84.1 104.8 108.3

Cyperus rotundus L. rhizome 27.5 52.8 55.8

Cornus officinalis Sieb. et Zucc. fruit 30.4 92.1 101 .6

Gentiana scabra Bunge root 42.2 107.4 108.6

Ligustrum lucidum Ait. fruit 97.6 58.1 98.4

Lithospermum erythrorhizon Sieb. et Zucc. root 43.8 103.8 1 1 1.3

Notopterygium incisium Ting root / rhizome 18.1 97.4 94.8

Paeonia lactiflora Pall. root 31 .8 62.2 100.7 8

1

Siesgesbeckia pubescens Makino herb 17.0 40.8 51.7 re^* > Spirodela polyrrhiza (L.) Scheid herb 100 79.3 96.6

Trichosanthes kirilowii Maxim root 1 12.9 108.1 1 16.1

Tripterygium wilfordii Hook. root 89.8 36.7 63.3

Zingiber officinale Roscoe rhizome 7.9 105.8 90.6

< 0.01 compared with vehicle treatment (one-way ANOVA, followed by Dunnett's t-test).

Table 2. Effects of Piper nigrum extract on the proliferation of melan-a cells counted with haemocytometer

Treatment to cells cell number (x 10^"4/ml)

Control 2.02

20 nM TPA 5.0*

Piper nigrum at 0.01 mg/ml 3.06*

Piper nigrum at 0.1 mg/ml 3.13*

*P < 0.01 compared with vehicle treatment (one-way ANOVA, followed by Dunnett's t-test).

s

OS

Table 3. Biological origin and the culture conditions of a panel of different cell types used in selectivity experiment.

Cell name biological origin optimum culture conditions

FBS Medium incubation (4days)

Melan-a normal epidermal melanoblasts from embryos of inbred 5% RPMI 1640 37°C,10%CO₂ C57BL mice

m Melan-c albino embryos of outbred LAC-MF strain mice 10% RPMI 1640 37°C,10%CO₂ a

H

H FM21E human foetal melanoblasts from epidermis (strain 21) 10% MCDB153 37°C,10%CO₂ ! melanoblast Λ to

FM21 E Human melanocytes derived from FM21E melanoblasts RPMI 1640 37°C,10%CO₂ melanocyte

SVK14 human keratinocytes 10% DMEM 37°C,10%CO₂ " O s

CSM14.1.4 neuronal cells from mesencephalin of rat 10% DMEM 34°C,5%CO₂

SCI fibroblastoids from neonatal murine skin 10% DMEM 37°C,10%CO₂

XB2 murine keratinocytes 10% DMEM 37°C,10%CO₂

B6F10 mouse melanoma 5% RPMI 1640 37^υC,10%CO₂

CACO2 human colon cancer 10% RPMI 1640 37°C,10%CO₂

IM9 human lymphoblastoid B cells 10% RPMI 1640 37°C, 5%CO₂

CΛ Swiss 3T3 mouse fibroblasts 10% DMDM 37°C, 5%CO² α a

H

H Human healthy human blood samples 10% DMEM 37°C, 5%CO₂ cj lymphocytes to t 3

re" σs

Table 4 Effects of piperine and TPA on the growth of a panel of cell types, (see Table 3 for details of cells) cell number as a % of control

Cell type piperine at the concentration of (μM) TPA at the concentration of

0.01 0.1 1 10 100 20nM 200nM

Melan-a ND 130* 169* 153* ND 295* ND

Melan-c 109 208* 198* 1 19* 137* 186* 222* fl FM21 E ND 101 101 1 19* 134* 153* ND a Melanoblast

H H FM21 E ND ND 98 143* 75* 98 102 human melanocytes SVK14 97 101 92 84* 23* 71 * 66*

CSM14.1.4 93 94 95 89 64* 85* 76* "

SCI 191 * 178* 175* 204* 190* 178* 199*

XB2 80 90 86 90 42* 96 99

B 16F 10 71 * 64* 47* 33* 0* 35* 55*

CACO2 103 99 102 81 * 34* 95 90

IM9 ND 101 103 69* ND ND ND

Swiss 3T3 1 13 104 106 102 51 * 185* 207*

Human ND 93 93 ND ND 282* ND lymphocytes

* P<0.05 compared to treatment with vehicle alone (one-way ANOVA followed by Dunnett's t-test); ND = not done.

Relative standard deviations of all values were less than 10% of the mean

n, a

H

H el H W to

re to

OS

Table 5: Effects of piperine on the proliferation and melanin production of human melanocytes cultured in petri dishes

Treatments Cellno.(xl0^"4)± % of control OD reading ± SD Melanin

SD after cultured for 5 (ETl InM) at 475 nm content/ 10 cells days

Cells only 17.71 ±6.16* 49.1 0.026 ± 0.0049* 0.23 ±0.001 μg

ETl (InM) 36.04 ±6.16 100.0 0.087 ± 0.044 0.46 ±0.01 μg α a

H ETl (InM) + 60.83 ±16.78 168.8 0.134 ±0.014* 0.42 ± 0.03 μg H piperine (1 μM) β i ETl (InM) + 78.96 ±5.63* 219.1 0.137 ±0.0085* 0.334 ±0.01 μg piperine (5 μM)

ETl (lnM) + 64.79 ±13.47* 179.8 0.139 ±0.028* 0.41 ±0.07μg piperine (10 μM) S

ETl (lnM) + 61.04 ±10.04 169.4 0.144 ±0.0046* 0.46 ±0.001 μg piperine (100 μM)

P < 0.05 when compared to ETl (1 nM) control (one way ANOVA, followed by Dunnett's t-test

Claims

1 . Use of piperine or an active analogue or derivative thereof for stimulating the proliferation of melanocytes.

2. Use of piperine or an active analogue or derivative thereof in the preparation of a medicament for re-pigmenting de-pigmented skin.

3. Use according to Claim 2, in which the de-pigmented skin is post traumatised de- pigmented skin.

4. Use of piperine or an active analogue or derivative thereof in the preparation of a medicament for the treatment of vitiligo.

5. Use of piperine or an active analogue or derivative thereof in the preparation of a medicament for enhancing or promoting the colouration of the skin.

6. An active analogue or derivative thereof for stimulating the proliferation of melanocytes

7. An active analogue or derivative of piperine for re-pigmenting de-pigmented skin.

8. An active analogue or derivative of piperine for the treatment of vitiligo.

9. An active analogue or derivative of piperine for enhancing or promoting the colouration of the skin.

10. Piperine or active analogue or derivative thereof according to any one of claims 6 to 9, wherein the active analogue or derivative is contained in a plant extract.

1 1. A method of cosmetically enhancing the colour of the skin comprising the administration to a recipient desiring enhanced skin colour of a cosmetically effective amount of piperine or an active analogue or derivative thereof.

12. A method according to Claim 1 1 , in which the method of cosmetically enhancing the colour of the skin is a method of tanning.

13. A method of re-pigmenting de-pigmented skin comprising the administration to a patient requiring said re-pigmentation a pharmaceutically effective amount of piperine or an active analogue or derivative thereof.

14. A method according to Claim 13, in which the de-pigmented skin is post traumatised de-pigmented skin.

15. A method according to Claim 13, which is a method of treating or preventing vitiligo.

16. A method according to any one of claims 1 1 to 15, wherein the piperine or analogue or derivative thereof is applied topically.

17. Use according to any one of claims 6 to 10 or a method according to any one of claims 11 or 12, in which the piperine or active derivative or analogue thereof is in the form of a plant extract.

18. A pharmaceutical composition comprising an active analogue or derivative of piperine and a pharmaceutically acceptable carrier.

19. A method of treating or preventing skin cancer in a human or non-human patient comprising the administration to said patient of a therapeutically effective amount of piperine or an active analogue or derivative thereof.

20. Use of piperine or an active analogue or derivative thereof in the preparation of a medicamant for the treatment of skin cancer.