WO2000000639A3 - Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin - Google Patents

Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin Download PDF

Info

Publication number
WO2000000639A3
WO2000000639A3 PCT/EP1999/004501 EP9904501W WO0000639A3 WO 2000000639 A3 WO2000000639 A3 WO 2000000639A3 EP 9904501 W EP9904501 W EP 9904501W WO 0000639 A3 WO0000639 A3 WO 0000639A3
Authority
WO
WIPO (PCT)
Prior art keywords
leptin
compound
response element
potentiates
mimics
Prior art date
Application number
PCT/EP1999/004501
Other languages
French (fr)
Other versions
WO2000000639A2 (en
Inventor
Michael Anthony Cawthorne
Valur Emilsson
Nicholas Michael Morton
Original Assignee
Univ Buckingham
Michael Anthony Cawthorne
Valur Emilsson
Nicholas Michael Morton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Buckingham, Michael Anthony Cawthorne, Valur Emilsson, Nicholas Michael Morton filed Critical Univ Buckingham
Priority to IL14007599A priority Critical patent/IL140075A0/en
Priority to EP99932753A priority patent/EP1092048A2/en
Priority to KR1020007015081A priority patent/KR20010053331A/en
Priority to AU49024/99A priority patent/AU4902499A/en
Priority to HU0102809A priority patent/HUP0102809A2/en
Priority to CA002335557A priority patent/CA2335557A1/en
Priority to PL99345382A priority patent/PL345382A1/en
Priority to BR9911643-0A priority patent/BR9911643A/en
Publication of WO2000000639A2 publication Critical patent/WO2000000639A2/en
Publication of WO2000000639A3 publication Critical patent/WO2000000639A3/en
Priority to NO20006541A priority patent/NO20006541L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A method for detection of a compound that mimics, potentiates or inhibits the physiological effect of leptin, which method comprises: (a) for a compound which mimics the physiological effect of the leptin, assessing the effect of the compound upon a leptin activated signal transducer and activator of transcription (STAT) DNA response element coupled to a reporter gene; or (b) for a compound which potentiates or inhibits the physiological effect of leptin, assessing the effect of the compound upon the response provided by leptin upon a leptin activated STAT DNA response element coupled to a reporter gene; wherein, the response element and the reporter are expressed in the human Caucasian colon epithelial cell line (CACO-2) or the response element and the reporter are expressed in CACO-2 cells, which cell line (the engineered cell line) is also transfected with a polypeptide which is capable of mediating or enhancing the stimulation by leptin of a leptin activated STAT DNA response element and contains the appropriate STAT proteins.
PCT/EP1999/004501 1998-06-30 1999-06-28 Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin WO2000000639A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
IL14007599A IL140075A0 (en) 1998-06-30 1999-06-28 Method for detection of a compound that mimics, potentiates or inhibits the physiological effect of leptin
EP99932753A EP1092048A2 (en) 1998-06-30 1999-06-28 Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin
KR1020007015081A KR20010053331A (en) 1998-06-30 1999-06-28 Method for Detecting of a Compound that Mimics, Potentiates or Inhibits the Physiological Effect of Leptin
AU49024/99A AU4902499A (en) 1998-06-30 1999-06-28 Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin
HU0102809A HUP0102809A2 (en) 1998-06-30 1999-06-28 Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin
CA002335557A CA2335557A1 (en) 1998-06-30 1999-06-28 Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin
PL99345382A PL345382A1 (en) 1998-06-30 1999-06-28 Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin
BR9911643-0A BR9911643A (en) 1998-06-30 1999-06-28 Process for detecting a compound that mimics, enhances or inhibits the physiological effect of leptin
NO20006541A NO20006541L (en) 1998-06-30 2000-12-21 Method for detecting a compound that mimics, potentiates or inhibits the physiological effect of leptin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9814199.7A GB9814199D0 (en) 1998-06-30 1998-06-30 Novel method
GB9814199.7 1998-06-30

Publications (2)

Publication Number Publication Date
WO2000000639A2 WO2000000639A2 (en) 2000-01-06
WO2000000639A3 true WO2000000639A3 (en) 2000-08-17

Family

ID=10834721

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004501 WO2000000639A2 (en) 1998-06-30 1999-06-28 Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin

Country Status (15)

Country Link
EP (1) EP1092048A2 (en)
KR (1) KR20010053331A (en)
CN (1) CN1316015A (en)
AR (1) AR020100A1 (en)
AU (1) AU4902499A (en)
BR (1) BR9911643A (en)
CA (1) CA2335557A1 (en)
GB (1) GB9814199D0 (en)
HU (1) HUP0102809A2 (en)
IL (1) IL140075A0 (en)
NO (1) NO20006541L (en)
PL (1) PL345382A1 (en)
TR (1) TR200003887T2 (en)
WO (1) WO2000000639A2 (en)
ZA (1) ZA200007689B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106636000A (en) * 2016-12-23 2017-05-10 广东圣赛生物科技有限公司 hESCs-TK cell line and building method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996038586A1 (en) * 1995-05-30 1996-12-05 Smithkline Beecham Plc Method for the detection of compounds that modulate the effects of the obese protein
WO1997035005A2 (en) * 1996-03-20 1997-09-25 Isis Innovation Limited Cftr gene regulator
WO1998020158A1 (en) * 1996-11-01 1998-05-14 Smithkline Beecham Plc Method for the detection of compounds that modulate the effects of the obese (ob) protein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996038586A1 (en) * 1995-05-30 1996-12-05 Smithkline Beecham Plc Method for the detection of compounds that modulate the effects of the obese protein
WO1997035005A2 (en) * 1996-03-20 1997-09-25 Isis Innovation Limited Cftr gene regulator
WO1998020158A1 (en) * 1996-11-01 1998-05-14 Smithkline Beecham Plc Method for the detection of compounds that modulate the effects of the obese (ob) protein

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GHILARDI N ET AL: "DEFECTIVE STAT SIGNALING BY THE LEPTIN RECEPTOR IN DIABETIC MICE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 93, 1 June 1996 (1996-06-01), pages 6231 - 6235, XP002030820, ISSN: 0027-8424 *
MORTON N M ET AL.: "Leptin signalling in pancreatic islets and clonal insulin-secreting cells", JOURNAL OF MOLECULAR ENDOCRINOLOGY, vol. 22, 1999, pages 173 - 184, XP000905489 *
SEIDEL H M ET AL: "SPACING OF PALINDROMIC HALF SITES AS A DETERMINANT OF SELECTIVE STAT (SIGNAL TRANSDUCERS AND ACTIVATORS OF TRANSCIPTION) DNA BINDING AND TRANSCIPTIONAL ACTIVITY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 92, 1 March 1995 (1995-03-01), pages 3041 - 3045, XP002009232, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
IL140075A0 (en) 2002-02-10
BR9911643A (en) 2001-03-20
NO20006541D0 (en) 2000-12-21
NO20006541L (en) 2000-12-21
KR20010053331A (en) 2001-06-25
CN1316015A (en) 2001-10-03
HUP0102809A2 (en) 2001-12-28
ZA200007689B (en) 2001-12-12
EP1092048A2 (en) 2001-04-18
AR020100A1 (en) 2002-04-10
AU4902499A (en) 2000-01-17
GB9814199D0 (en) 1998-08-26
CA2335557A1 (en) 2000-01-06
PL345382A1 (en) 2001-12-17
TR200003887T2 (en) 2001-06-21
WO2000000639A2 (en) 2000-01-06

Similar Documents

Publication Publication Date Title
EP1244808A4 (en) Method of screening multiply transformed cells using bicistronic expression of fluorescent proteins
AU2610101A (en) Methods and systems for monitoring intracellular binding reactions
WO2003039483A3 (en) An enzyme-based system and sensor for measuring acetone
AU6524100A (en) An automated point of care detection system including complete sample processingcapabilities
AU1733100A (en) Devices and methods for performing blood coagulation assays by piezoelectric sensing
WO2001081919A3 (en) Gastric inhibitory polypeptide diagnostic test for detecting susceptibility to type-2 diabetes, impaired glucose tolerance, or impaired fasting glucose
CA2262920A1 (en) Method for identifying compounds affecting rna/rna binding protein interactions
AU2003287527A1 (en) Single cell analysis of membrane molecules
WO2003100413A3 (en) Surface acoustic wave sensors and methods for detecting target analytes
EP2357478A3 (en) Diagnosis of disease
WO2005113838A3 (en) Detection of protein translocation by beta-galactosidase reporter fragment complementation
AUPO128096A0 (en) Biosensors
MX9709360A (en) Method for the detection of compounds that modulate the effects of the obese protein.
ATE377753T1 (en) DETECTION OF DNA BINDING PROTEINS
WO2000000639A3 (en) Method for detecting of a compound that mimics, potentiates or inhibits the physiological effect of leptin
WO2003095606A3 (en) A t cell subpopulation regulating gut immunity
CA2407760A1 (en) Methods for diagnosis of low grade astrocytoma
TR199900963T2 (en) Method for detecting compounds that conform to the effects of the obese (OB) protein.
AU7828698A (en) Detection of loss of the wild-type (hu)bub1 gene
WO2002037077A3 (en) Screening methods based on transcriptional regulation analysis system using transformed cell lines
AU3063699A (en) Isolated mammalian membrane protein genes and related reagents
WO1995028482A3 (en) Dna spacer regulatory elements responsive to cytokines and methods for their use
WO2007047985A3 (en) Trpm5 based assays and the use thereof for the identification of modulators of sweet, bitter or umami (savory) taste
ATE253224T1 (en) DETECTION OF CELLS USING SPECIFIC CELL WALL-BINDING DOMAINS (CBD) OF CELL WALL-BINDING PROTEINS
WO2002050104A3 (en) Methods and cells for detecting modulators of rgs proteins

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99808215.5

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 140075

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: 49024/99

Country of ref document: AU

Ref document number: 1999932753

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2000/07689

Country of ref document: ZA

Ref document number: 200007689

Country of ref document: ZA

WWE Wipo information: entry into national phase

Ref document number: IN/PCT/2000/00766/MU

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 2000/03887

Country of ref document: TR

WWE Wipo information: entry into national phase

Ref document number: PV2000-4897

Country of ref document: CZ

ENP Entry into the national phase

Ref document number: 2000 557392

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 1020007015081

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: PA/a/2001/000149

Country of ref document: MX

WWP Wipo information: published in national office

Ref document number: 1999932753

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: PV2000-4897

Country of ref document: CZ

WWP Wipo information: published in national office

Ref document number: 1020007015081

Country of ref document: KR

ENP Entry into the national phase

Ref document number: 2335557

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: CA

WWR Wipo information: refused in national office

Ref document number: PV2000-4897

Country of ref document: CZ

WWW Wipo information: withdrawn in national office

Ref document number: 1999932753

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1020007015081

Country of ref document: KR