WO1999066324A3 - A bioluminescence resonance energy transfer (bret) system and its use - Google Patents
A bioluminescence resonance energy transfer (bret) system and its use Download PDFInfo
- Publication number
- WO1999066324A3 WO1999066324A3 PCT/CA1999/000561 CA9900561W WO9966324A3 WO 1999066324 A3 WO1999066324 A3 WO 1999066324A3 CA 9900561 W CA9900561 W CA 9900561W WO 9966324 A3 WO9966324 A3 WO 9966324A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- bioluminescent protein
- fluorophore
- protein
- bret
- energy transfer
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/72—Fusion polypeptide containing domain for protein-protein interaction containing SH2 domain
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
Abstract
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE69928255T DE69928255T2 (en) | 1998-06-16 | 1999-06-16 | BIOLUMINESCENT RESONANCE ENERGY TRANSFER SYSTEM (BRET) AND ITS APPLICATION |
CA002335305A CA2335305C (en) | 1998-06-16 | 1999-06-16 | A bioluminescence resonance energy transfer (bret) system and its use |
EP99957096A EP1088233B9 (en) | 1998-06-16 | 1999-06-16 | A bioluminescence resonance energy transfer (bret) system and its use |
AU42542/99A AU4254299A (en) | 1998-06-16 | 1999-06-16 | A bioluminescence resonance energy transfer (bret) system and its use |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8962398P | 1998-06-16 | 1998-06-16 | |
US60/089,623 | 1998-06-16 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO1999066324A2 WO1999066324A2 (en) | 1999-12-23 |
WO1999066324A3 true WO1999066324A3 (en) | 2000-04-06 |
WO1999066324A9 WO1999066324A9 (en) | 2000-07-13 |
Family
ID=22218673
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CA1999/000561 WO1999066324A2 (en) | 1998-06-16 | 1999-06-16 | A bioluminescence resonance energy transfer (bret) system and its use |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1088233B9 (en) |
AU (1) | AU4254299A (en) |
CA (1) | CA2335305C (en) |
DE (1) | DE69928255T2 (en) |
WO (1) | WO1999066324A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110330558B (en) * | 2019-08-05 | 2020-09-22 | 博迈德生物科技(固安)有限公司 | Yellow fluorescent protein and application thereof |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6528271B1 (en) | 1997-06-05 | 2003-03-04 | Duke University | Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia |
CN1160470C (en) * | 1998-09-03 | 2004-08-04 | 洛马林达大学 | Method for studying protein interactions i(invivo) |
AU2137101A (en) * | 1999-12-22 | 2001-07-03 | Biosignal Packard Inc. | A bioluminescence resonance energy transfer (bret) fusion molecule and method ofuse |
JP2001327296A (en) * | 2000-03-15 | 2001-11-27 | Japan Science & Technology Corp | Method for detecting interaction between protein and protein |
AU781992B2 (en) * | 2000-04-04 | 2005-06-23 | Medical Research Council | Methods involving induced dimerisation by cellular components |
US7163800B2 (en) | 2000-11-03 | 2007-01-16 | Molecular Devices Corporation | Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity |
US7018812B2 (en) | 2000-11-03 | 2006-03-28 | Duke University | Modified G-protein coupled receptors |
US20020073441A1 (en) * | 2000-12-13 | 2002-06-13 | Ross Brian D. | Compositions and methods for detecting proteolytic activity |
WO2003004643A2 (en) | 2001-07-03 | 2003-01-16 | Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw | Reversed mammalian protein-protein interaction trap |
FR2826666B1 (en) * | 2001-07-02 | 2004-07-02 | Novaleads | METHOD FOR SELECTING BIO-ACTIVE AGENTS BY LUMINESCENCE COUPLING AND LIVING CELLULAR SYSTEM FOR CARRYING OUT SAID METHOD |
ATE509120T1 (en) | 2001-09-18 | 2011-05-15 | Carnegie Inst Of Washington | FUSION PROTEINS FOR DETECTING ANALYTES |
US7268229B2 (en) | 2001-11-02 | 2007-09-11 | Promega Corporation | Compounds to co-localize luminophores with luminescent proteins |
US7846671B2 (en) | 2002-01-28 | 2010-12-07 | Bristol-Myers Squibb Company | Methods of screening for agents that modulate the interaction of RGS and Gαq and urinary incontinence |
FR2836475B1 (en) | 2002-02-26 | 2007-02-23 | Aventis Pharma Sa | METHOD FOR DETECTING LEPTIN RECEPTOR LIGANDS |
EP1579212B1 (en) | 2002-10-25 | 2008-12-31 | MDS Analytical Technologies (US) Inc. | Methods of identifying reduced internalization transmembrane receptor agonists |
WO2004052934A1 (en) * | 2002-12-12 | 2004-06-24 | National Institute Of Advanced Industrial Science And Technology | Monitor protein for measuring processing of protein |
AU2003293959A1 (en) | 2002-12-20 | 2004-07-14 | Julius-Maximilians-Uni Versitat Wurzburg | Millisecond activation switch for seven-transmembrane proteins |
US8883486B2 (en) | 2004-05-04 | 2014-11-11 | Universite De Montreal | Arrestin biosensor |
CA2607015C (en) | 2004-05-04 | 2016-07-05 | Universite De Montreal | Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same |
EP2400030B1 (en) * | 2004-05-18 | 2016-01-20 | The Board of Trustees of the Leland Stanford Junior University | Detection of protein translocation by beta-galactosidase reporter fragment complementation |
WO2006086883A1 (en) * | 2005-02-16 | 2006-08-24 | Universite De Montreal | Biosensors for monitoring receptor-mediated g-protein activation |
KR20080050584A (en) | 2005-08-19 | 2008-06-09 | 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 | Arachnocampa luciferases |
US7910087B2 (en) | 2007-03-02 | 2011-03-22 | University Of Massachusetts | Luciferins |
DE102010018878B4 (en) | 2009-04-30 | 2013-09-26 | Julius-Maximilians-Universität Würzburg | New cell line for the fluorescence-based detection of functionally active antibodies and autoantibodies against the beta1-adrenergic receptor |
US9127282B2 (en) | 2011-06-24 | 2015-09-08 | University Of Houston System | Fusion proteins of bacterial luciferase as multicolor luminescent sensors |
RU2497128C2 (en) * | 2011-12-30 | 2013-10-27 | Федеральное Государственное Автономное Образовательное Учреждение Высшего Профессионального Образования "Сибирский Федеральный Университет" | Method for simultaneous determination of two analytes via bioluminescent molecular microanalysis |
US10067149B2 (en) | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
WO2014093677A1 (en) | 2012-12-12 | 2014-06-19 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
US20180251765A1 (en) * | 2015-09-08 | 2018-09-06 | BellBrook Labs | High-throughput split aptamer screening assay |
CN107164457B (en) * | 2017-04-26 | 2021-01-26 | 北京工商大学 | High-throughput screening method of broad-spectrum lysozyme |
EP3882632A1 (en) * | 2020-03-18 | 2021-09-22 | Universite De Bordeaux | Novel ion conducting channel fusion subunits and methods of use thereof |
-
1999
- 1999-06-16 CA CA002335305A patent/CA2335305C/en not_active Expired - Lifetime
- 1999-06-16 EP EP99957096A patent/EP1088233B9/en not_active Expired - Lifetime
- 1999-06-16 AU AU42542/99A patent/AU4254299A/en not_active Abandoned
- 1999-06-16 WO PCT/CA1999/000561 patent/WO1999066324A2/en active IP Right Grant
- 1999-06-16 DE DE69928255T patent/DE69928255T2/en not_active Expired - Lifetime
Non-Patent Citations (3)
Title |
---|
KOJIMA S ET AL: "Mechanism of the Redox Reaction of the Aequorea Green Fluorescent Protein (GFP)", TETRAHEDRON LETTERS,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 38, no. 16, pages 2875-2878, XP004058517, ISSN: 0040-4039 * |
WU P ET AL: "RESONANCE ENERGY TRANSFER: METHODS AND APPLICATIONS", ANALYTICAL BIOCHEMISTRY,US,ACADEMIC PRESS, SAN DIEGO, CA, vol. 218, pages 1-13, XP002033685, ISSN: 0003-2697 * |
XU, YAO ET AL: "A bioluminescence resonance energy transfer ( BRET ) system: application to interacting circadian clock proteins", PROC. NATL. ACAD. SCI. U. S. A. (1999), 96(1), 151-156, XP002127270 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110330558B (en) * | 2019-08-05 | 2020-09-22 | 博迈德生物科技(固安)有限公司 | Yellow fluorescent protein and application thereof |
Also Published As
Publication number | Publication date |
---|---|
CA2335305C (en) | 2006-05-23 |
CA2335305A1 (en) | 1999-12-23 |
EP1088233B1 (en) | 2005-11-09 |
DE69928255D1 (en) | 2005-12-15 |
WO1999066324A2 (en) | 1999-12-23 |
EP1088233B9 (en) | 2006-05-17 |
DE69928255T2 (en) | 2006-11-09 |
AU4254299A (en) | 2000-01-05 |
EP1088233A2 (en) | 2001-04-04 |
WO1999066324A9 (en) | 2000-07-13 |
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