WO1999064624A3 - Method of generating nucleic acid hybrids for mutation analysis - Google Patents

Method of generating nucleic acid hybrids for mutation analysis Download PDF

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Publication number
WO1999064624A3
WO1999064624A3 PCT/GB1999/001691 GB9901691W WO9964624A3 WO 1999064624 A3 WO1999064624 A3 WO 1999064624A3 GB 9901691 W GB9901691 W GB 9901691W WO 9964624 A3 WO9964624 A3 WO 9964624A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequences
primers
hybrid molecule
mutation analysis
nucleic acid
Prior art date
Application number
PCT/GB1999/001691
Other languages
French (fr)
Other versions
WO1999064624A2 (en
Inventor
Andrew Wallace
Original Assignee
Central Manchester Healthcare
Andrew Wallace
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Central Manchester Healthcare, Andrew Wallace filed Critical Central Manchester Healthcare
Priority to AU53775/99A priority Critical patent/AU5377599A/en
Priority to CA002330252A priority patent/CA2330252A1/en
Priority to JP2000553614A priority patent/JP2002517258A/en
Priority to EP99939503A priority patent/EP1086250A2/en
Publication of WO1999064624A2 publication Critical patent/WO1999064624A2/en
Publication of WO1999064624A3 publication Critical patent/WO1999064624A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of producing a hybrid DNA molecule allowing the assembly of sequences x1, x2.....xn where n is greater than or equal to 3 (e.g. give sequences) from diverse locations into a hybrid molecule for the purpose of mutation analysis. The method comprising the steps of: (1) providing in a single reaction mixture: (a) the sequences x1, x2.......xn and their complementary sequences x1', x2'.........xn', to be assembled into the hybrid molecule; (b) for each pair of complementary sequences defined in (a) a respective pair of PCR primers each having a priming sequence and which are such that the primers hybridising to the 3' ends of any two sequences (xi, x'(i+1)), where i is 1 to (n-1), have specifically complementary linker sequences; (2) effecting a first stage PCR reaction in which those primers provided with linker sequences are present in limiting concentrations; and (3) effecting a second stage PCR reaction using a single pair of primers one of which provides the 5'-end of the sense strand and other of which provides the 3'-end of the anti-sense strand of the required hybrid molecule; whereby said hybrid molecule is generated.
PCT/GB1999/001691 1998-06-12 1999-06-14 Method of generating nucleic acid hybrids for mutation analysis WO1999064624A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU53775/99A AU5377599A (en) 1998-06-12 1999-06-14 Nucleic acids
CA002330252A CA2330252A1 (en) 1998-06-12 1999-06-14 Method of generating nucleic acid hybrids for mutation analysis
JP2000553614A JP2002517258A (en) 1998-06-12 1999-06-14 Methods for generating nucleic acid hybrids for mutation analysis
EP99939503A EP1086250A2 (en) 1998-06-12 1999-06-14 Method of generating nucleic acid hybrids for mutation analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9812674.1A GB9812674D0 (en) 1998-06-12 1998-06-12 Nucleic acids
GB9812674.1 1998-06-12

Publications (2)

Publication Number Publication Date
WO1999064624A2 WO1999064624A2 (en) 1999-12-16
WO1999064624A3 true WO1999064624A3 (en) 2000-09-14

Family

ID=10833627

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001691 WO1999064624A2 (en) 1998-06-12 1999-06-14 Method of generating nucleic acid hybrids for mutation analysis

Country Status (6)

Country Link
EP (1) EP1086250A2 (en)
JP (1) JP2002517258A (en)
AU (1) AU5377599A (en)
CA (1) CA2330252A1 (en)
GB (1) GB9812674D0 (en)
WO (1) WO1999064624A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
EP2351853A1 (en) * 2000-06-06 2011-08-03 Life Technologies Corporation Method and devices for multiplexing amplification reactions
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
EP3000899A1 (en) 2002-12-04 2016-03-30 Applied Biosystems, LLC Multiplex amplification of polynucleotides
HU229967B1 (en) 2011-12-20 2015-03-30 Kps Diagnosztika Zrt Method for determining the sequence of fragmented nucleic acids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1992015678A1 (en) * 1991-03-01 1992-09-17 Stratagene Pcr generated dicistronic dna molecules for producing antibodies
WO1999016904A1 (en) * 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1992015678A1 (en) * 1991-03-01 1992-09-17 Stratagene Pcr generated dicistronic dna molecules for producing antibodies
WO1999016904A1 (en) * 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NEWTON ET AL: "PCR, 2. Auflage", 1997, SPEKTRUM AKADEMISCHER VERLAG, HEIDELBERG, XP002134140 *
SANDHU ET AL: "Dual assymetric PCR: One-step construction of synthetic genes", BIOTECHNIQUES, vol. 12, no. 1, 1992, pages 14 - 16, XP002134139 *

Also Published As

Publication number Publication date
WO1999064624A2 (en) 1999-12-16
JP2002517258A (en) 2002-06-18
CA2330252A1 (en) 1999-12-16
GB9812674D0 (en) 1998-08-12
EP1086250A2 (en) 2001-03-28
AU5377599A (en) 1999-12-30

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