WO1999064575A3 - Treating cells - Google Patents

Treating cells Download PDF

Info

Publication number
WO1999064575A3
WO1999064575A3 PCT/GB1999/001845 GB9901845W WO9964575A3 WO 1999064575 A3 WO1999064575 A3 WO 1999064575A3 GB 9901845 W GB9901845 W GB 9901845W WO 9964575 A3 WO9964575 A3 WO 9964575A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
treating cells
recognition
recognition agent
agent
Prior art date
Application number
PCT/GB1999/001845
Other languages
French (fr)
Other versions
WO1999064575A2 (en
Inventor
David Rickwood
Original Assignee
Immunoporation Ltd
David Rickwood
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9812519.8A external-priority patent/GB9812519D0/en
Application filed by Immunoporation Ltd, David Rickwood filed Critical Immunoporation Ltd
Priority to US09/719,325 priority Critical patent/US6344357B1/en
Priority to EP99955500A priority patent/EP1088063B1/en
Priority to AU42816/99A priority patent/AU4281699A/en
Priority to DE69928254T priority patent/DE69928254D1/en
Priority to AT99955500T priority patent/ATE309340T1/en
Publication of WO1999064575A2 publication Critical patent/WO1999064575A2/en
Publication of WO1999064575A3 publication Critical patent/WO1999064575A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Dicing (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)

Abstract

Provided is a method for introducing a substance into a cell, which method comprises: (a) contacting the cell with a recognition agent to bind the recognition agent to a recognition site on the surface of the cell; and (b) separating the recognition agent from the cell thereby forming a hole in the surface of the cell. Kits for use in such methods are also provided.
PCT/GB1999/001845 1998-06-10 1999-06-10 Treating cells WO1999064575A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US09/719,325 US6344357B1 (en) 1998-06-10 1999-06-10 Treating cells
EP99955500A EP1088063B1 (en) 1998-06-10 1999-06-10 Treating cells
AU42816/99A AU4281699A (en) 1998-06-10 1999-06-10 Treating cells
DE69928254T DE69928254D1 (en) 1998-06-10 1999-06-10 TREATMENT OF CELLS
AT99955500T ATE309340T1 (en) 1998-06-10 1999-06-10 TREATMENT OF CELLS

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9812519.8 1998-06-10
GBGB9812519.8A GB9812519D0 (en) 1998-06-10 1998-06-10 Treating cells
GB9912851.4 1999-06-02
GB9912851A GB2338483A (en) 1998-06-10 1999-06-02 Forming holes in cell membranes

Publications (2)

Publication Number Publication Date
WO1999064575A2 WO1999064575A2 (en) 1999-12-16
WO1999064575A3 true WO1999064575A3 (en) 2000-02-03

Family

ID=26313834

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001845 WO1999064575A2 (en) 1998-06-10 1999-06-10 Treating cells

Country Status (5)

Country Link
EP (1) EP1088063B1 (en)
AT (1) ATE309340T1 (en)
AU (1) AU4281699A (en)
DE (1) DE69928254D1 (en)
WO (1) WO1999064575A2 (en)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
C.P. RUBBI ET AL.: "Evidence of surface detachment during incubation of cells with immunomagnetic beads", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 166, 1993, pages 233 - 241, XP002123015 *
M.R. LIEBER ET AL.: "The mechanism of osmotic transfection of avian embryonic erythrocytes: analysis of a system for studying developmental gene expression", JOURNAL OF CELL BIOLOGY, vol. 105, 1987, pages 1055 - 1065, XP002123012 *
S. KURATA ET AL.: "The laser method for efficient introduction of foreign DNA into cultured cells", EXPERIMENTAL CELL RESEARCH, vol. 162, 1986, pages 372 - 378, XP002123013 *
Y. GUO ET AL.: "lASER-MEDIATED GENE TRANSFER IN RICE", PHYSIOLOGIA PLANTARUM, vol. 93, 1995, pages 19 - 24, XP002123014 *

Also Published As

Publication number Publication date
AU4281699A (en) 1999-12-30
DE69928254D1 (en) 2005-12-15
WO1999064575A2 (en) 1999-12-16
ATE309340T1 (en) 2005-11-15
EP1088063B1 (en) 2005-11-09
EP1088063A2 (en) 2001-04-04

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