WO1999062490A1 - Small molecule sulfonamide hair growth compositions and uses - Google Patents
Small molecule sulfonamide hair growth compositions and uses Download PDFInfo
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- WO1999062490A1 WO1999062490A1 PCT/US1998/011253 US9811253W WO9962490A1 WO 1999062490 A1 WO1999062490 A1 WO 1999062490A1 US 9811253 W US9811253 W US 9811253W WO 9962490 A1 WO9962490 A1 WO 9962490A1
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- HZECOIWROWDTJD-UHFFFAOYSA-N Cc(cc1)ccc1S(N(CCCC1)C1C(OCCCCc1ccccc1)=O)(=O)=O Chemical compound Cc(cc1)ccc1S(N(CCCC1)C1C(OCCCCc1ccccc1)=O)(=O)=O HZECOIWROWDTJD-UHFFFAOYSA-N 0.000 description 1
- XUAAPZIIRVZOLY-UHFFFAOYSA-N Cc1cccc(CS(N(CCCC2)C2C(OC(CCCc2ccccc2)CCCc2ccccc2)=O)(=O)=O)c1 Chemical compound Cc1cccc(CS(N(CCCC2)C2C(OC(CCCc2ccccc2)CCCc2ccccc2)=O)(=O)=O)c1 XUAAPZIIRVZOLY-UHFFFAOYSA-N 0.000 description 1
- KQLRLYLKDPJCLG-UHFFFAOYSA-N O=C(C(CCC1)N1S(Cc1ccccc1)(=O)=O)OCCCc1cnccc1 Chemical compound O=C(C(CCC1)N1S(Cc1ccccc1)(=O)=O)OCCCc1cnccc1 KQLRLYLKDPJCLG-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
- A61K31/401—Proline; Derivatives thereof, e.g. captopril
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/16—Amides, e.g. hydroxamic acids
- A61K31/18—Sulfonamides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
- A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/445—Non condensed piperidines, e.g. piperocaine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/466—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfonic acid derivatives; Salts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4926—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having six membered rings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
Definitions
- This invention relates to pharmaceutical compositions and methods for treating alopecia and promoting hair growth using low molecular weight, small molecule sulfonamides .
- Hair loss occurs in a variety of situations. These situations include male pattern alopecia, alopecia senilis, alopecia areata, diseases accompanied by basic skin lesions or tumors, and systematic disorders such as nutritional disorders and internal secretion disorders.
- the mechanisms causing hair loss are very complicated, but in some instances can be attributed to aging, genetic disposition, the activation of male hormones, the loss of blood supply to hair follicles, and scalp abnormalities.
- the immunosuppressant drugs FK506, rapamycin and cyclosporin are well known as potent T-cell specific immunosuppressants, and are effective against graft rejection after organ transplantation. It has been reported that topical, but not oral, application of FK506 (Yamamoto et al . , J. Invest. Dermatol., 1994, 102, 160-164; Jiang et al . , J. Invest. Dermatol. 1995, 104, 523-525) and cyclosporin (Iwabuchi et al . , J. Dermatol. Sci. 1995, 9, 64-69) stimulates hair growth in a dose-dependent manner.
- alopecia areata One form of hair loss, alopecia areata, is known to be associated with autoimmune activities; hence, topically administered immunomodulatory compounds are expected to demonstrate efficacy for treating that type of hair loss.
- the hair growth stimulating effects of FK506 have been the subject of an international patent filing covering FK506 and structures related thereto for hair growth stimulation (Honbo et al . , EP 0 423 714 A2) .
- Honbo et al discloses the use of relatively large tricyclic compounds, known for their immunosuppressive effects, as hair revitalizing agents.
- FK506 and related agents are disclosed in many U.S. patents (Goulet et al . , U.S. Patent No. 5,258,389; Luly et al . , U.S. Patent No. 5,457,111; Goulet et al . , U.S. Patent No. 5,532,248; Goulet et al . , U.S. Patent No. 5,189,042; and Ok et al . , U.S. Patent No. 5,208,241; Rupprecht et al . , U.S. Patent No. 5,284,840; Organ et al . , U.S. Patent No. 5,284,877).
- immunosuppressive compounds by definition suppress the immune system and also exhibit other toxic side effects. Accordingly, there is a need for non-immunosuppressant, small molecule compounds which are useful as hair revitalizing compounds .
- the present invention relates to a method for treating alopecia or promoting hair growth in an animal, which comprises administering to said animal an effective amount of a small molecule sulfonamide.
- the present invention further relates to a pharmaceutical composition which comprises: (i) an effective amount of a small molecule sulfonamide for treating alopecia or promoting hair growth in an animal; and (ii) a pharmaceutically acceptable carrier.
- the small molecule sulfonamides used in the inventive methods and pharmaceutical compositions may be immunosuppressive, but are preferably non- immunosuppressive compounds having an affinity for FKBP-type immunophilins, particularly FKBP12.
- Non- immunosuppressive compounds as their name suggests, do not exert any significant immunosuppressive activity.
- FIG. 1 is a photograph of mice treated with a vehicle after six weeks.
- FIG. 1 shows that less than 3% of the shaved area is covered with new hair growth when the vehicle (control) is administered.
- FIG. 2 is a photograph of mice treated with 10 ⁇ M of a related neuroimmunophilin FKBP ligand, GPI 1044, after six weeks.
- FIG. 2 shows that 90% of the shaved area is covered with new hair growth when GPI 1044 is administered.
- FIG. 3 is a photograph of mice treated with 10 ⁇ M of another related neuroimmunophilin FKBP ligand, GPI
- FIG. 3 shows that 90% of the shaved area is covered with new hair growth when GPI 1116 is administered.
- FIG. 4 is a photograph of mice treated with 3 ⁇ M of a third related neuroimmunophilin FKBP ligand, GPI
- FIG. 3 shows that 90% of the shaved area is covered with new hair growth when GPI 1102 is administered.
- FIG. 5 is a bar graph plotting the hair growth scores of unshaven animals and shaven animals treated with a vehicle, GPI 1044 (1 ⁇ M, 3 ⁇ M and 10 ⁇ M) , GPI 1116 (1 ⁇ M and 10 ⁇ M) , and GPI 1102 (1 ⁇ M and 3 ⁇ M) .
- FIG. 6 is a bar graph depicting the relative hair growth indices for C57 Black 6 mice treated with a vehicle, FK506, and related neuroimmunophilin FKBP ligands 14 days after treatment with each identified compound. Figure 6 demonstrates the remarkable early hair growth promoted by neuroimmunophilin FKBP ligands .
- Alopecia refers to deficient hair growth and partial or complete loss of hair, including without limitation androgenic alopecia (male pattern baldness), toxic alopecia, alopecia senilis, alopecia areata, alopecia pelada and trichotillomania .
- Alopecia results when the pilar cycle is disturbed. The most frequent phenomenon is a shortening of the hair growth or anagen phase due to cessation of cell proliferation. This results in an early onset of the catagen phase, and consequently a large number of hairs in the telogen phase during which the follicles are detached from the dermal papillae, and the hairs fall out.
- Alopecia has a number of etiologies, including genetic factors, aging, local and systemic diseases, febrile conditions, mental stresses, hormonal problems, and secondary effects of drugs.
- B is 3-Phenylpropyl
- D is 3-Phenylpropyl
- L is Phenyl
- GPI 1102 refers to 4 -phenyl -1- (3-phenylpropyl) butyl 1- (3 , 3-dimethyl-2-oxopentanoyl) - 2 - piperidinecarboxylate .
- GPI 1116 refers to 1-phenethyl -3-phenylpropyl 1 - ( 3 , 3 -dimethyl -2 -oxopentanoyl ) - 2 - piperidinecarboxylate .
- “Isomers” refer to different compounds that have the same molecular formula. “Stereoisomers” are isomers that differ only in the way the atoms are arranged in space. "Enantiomers” are a pair of stereoisomers that are non-superimposable mirror images of each other. “Diastereoisomers” are stereoisomers which are not mirror images of each other. “Racemic mixture” means a mixture containing equal parts of individual enantiomers. “Non-racemic mixture” is a mixture containing unequal parts of individual enantiomers or stereoisomers.
- “Pharmaceutically acceptable salt, ester, or solvate” refers to a salt, ester, or solvate of a subject compound which possesses the desired pharmacological activity and which is neither biologically nor otherwise undesirable.
- a salt, ester, or solvate can be formed with inorganic acids such as acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphorate, camphor sul fonat e , cyclopentanepropionate, digluconate, dodecylsulf te, ethanesulfonate, fumarate, glucoheptanoate, gluconate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2- hydroxyethanesulfonate , lactate,
- base salts, esters, or solvates include ammonium salts; alkali metal salts, such as sodium and potassium salts; alkaline earth metal salts, such as calcium and magnesium salts; salts with organic bases, such as dicyclohexylamine salts; N-methyl-D-glucamine; and salts with amino acids, such as arginine, lysine, and so forth.
- the basic nitrogen-containing groups can be quarternized with such agents as lower alkyl halides, such as methyl, ethyl, propyl, and butyl chlorides, bromides, and iodides; dialkyl sulfates, such as dimethyl, diethyl, dibutyl , and diamyl sulfates; long chain halides, such as decyl, lauryl, myristyl, and stearyl chlorides, bromides, and iodides; aralkyl halides, such as benzyl and phenethyl bromides; and others. Water or oil-soluble or dispersible products are thereby obtained.
- lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides, and iodides
- dialkyl sulfates such as dimethyl, diethyl, dibutyl , and diamyl sulf
- Palm cycle refers to the life cycle of hair follicles, and includes three phases:
- the telogen phase the rest period when hair progressively separates and finally falls out which, insofar as scalp hair is concerned, lasts about three to four months.
- 80 to 90 percent of the follicles are in the anagen phase, less than 1 percent being in the catagen phase, and the rest being in the telogen phase.
- hair is uniform in diameter with a slightly bulbous, non-pigmented root.
- hair has a large colored bulb at its root .
- “Promoting hair growth” refers to maintaining, inducing, stimulating, accelerating, or revitalizing the germination of hair.
- Treating alopecia refers to:
- Terminal hair is coarse, pigmented, long hair in which the bulb of the hair follicle is seated deep in the dermis.
- Vellus hair is fine, thin, non-pigmented short hair in which the hair bulb is located superficially in the dermis. As alopecia progresses, the hairs change from the terminal to the vellus type.
- the present invention relates to a method for treating alopecia or promoting hair growth in an animal, which comprises administering to said animal an effective amount of a small molecule sulfonamide.
- the inventive method is particularly useful for treating male pattern alopecia, alopecia senilis, alopecia areata, alopecia resulting from skin lesions or tumors, alopecia resulting from cancer therapy such as chemotherapy and radiation, and alopecia resulting from systematic disorders such as nutritional disorders and internal secretion disorders.
- the present invention also relates to a pharmaceutical composition
- a pharmaceutical composition comprising:
- the sulfonamides used in the methods and pharmaceutical compositions of the present invention are low molecular weight, small molecule compounds having an affinity for FKBP-type immunophilins, such as FKBP12.
- FKBP-type immunophilins such as FKBP12.
- FKBP12 FKBP12
- a sulfonamide binds to an FKBP-type immunophilin, it has been found to inhibit the prolyl- peptidyl cis - trans isomerase, or rotamase, activity of the binding protein. Unexpectedly, the compounds have also been found to stimulate hair growth. These rotamase inhibiting compounds are non- immunosuppressive. Examples of useful compounds are set forth below.
- An exemplary small molecule sulfonamide is a compound of Formula I
- A is CH 2 , 0, NH, or N- (C 1 -C 4 alkyl);
- B and D are independently Ar, hydrogen, C ⁇ C g straight or branched chain alkyl, or C 2 -C 6 straight or branched chain alkenyl, wherein said alkyl or alkenyl is unsubstituted or substituted with C 5 -C 7 cycloalkyl, C 5 -C 7 cycloalkenyl or Ar, and wherein one or two carbon atom(s) of said alkyl or alkenyl may be substituted with one or two heteroatom (s) independently selected from the group consisting of O, S, SO, and S0 2 in chemically reasonable substitution patterns, or
- Q is hydrogen, straight or branched chain alkyl, or C 2 -C 6 straight or branched chain alkenyl ;
- T is Ar or C 5 -C 7 cycloalkyl substituted at positions 3 and 4 with one or more substituent (s) independently selected from the group consisting of hydrogen, hydroxy,
- Ar is selected from the group consisting of phenyl, 1-napthyl, 2 -naphthyl, 2 -furyl, 3 -furyl, 2- thienyl, 3 -thienyl, 2-pyridyl, 3 -pyridyl, 4-pyridyl, monocyclic and bicyclic heterocyclic ring systems with individual ring sizes being 5 or 6 which contain in either or both rings a total of 1-4 heteroatoms independently selected from the group consisting of O, N, and S; wherein Ar contains 1-3 substituent (s) independently selected from the group consisting of hydrogen, halo, hydroxy, nitro, trifluoromethyl, trifluoromethoxy, straight or branched chain alkyl, C 2 -C 6 straight or branched chain alkenyl, O- (C
- E is Ci-Cg straight or branched chain alkyl, C 2 -C 6 straight or branched chain alkenyl, C 5 -C 7 cycloalkyl, C 5 -C 7 cycloalkenyl substituted with C 1 -C 4 straight or branched chain alkyl or C 2 -C 4 straight or branched chain alkenyl, (C 2 -C 4 alkyl or C 2 -C 4 alkenyl) -Ar, or Ar; J is hydrogen, C t or C 2 alkyl, or benzyl; K is C t - C 4 straight or branched chain alkyl, benzyl, or cyclohexylmethyl ; or J and K are taken together to form a 5-7 membered heterocyclic ring which is substituted with 0, S, SO, or S0 2 ; n is 0 to 3; and the stereochemistry at carbon positions 1 and 2 is R or S.
- FORMULA II
- B is selected from the group consisting of hydrogen, benzyl, 2- phenylethyl, and 3-phenylpropyl;
- D is selected from the group consisting of phenyl, 3-phenylpropyl, 3-phenoxyphenyl , and 4- phenoxyphenyl
- E is selected from the group consisting of phenyl , 4-methylphenyl , 4-methoxyphenyl , 2 -thienyl , 2 , 4 , 6-triisopropylphenyl , 4 -fluorophenyl , 3- methoxyphenyl , 2-methoxyphenyl , 3 , 5-dimethoxyphenyl , 3,4, 5-trimethoxyphenyl, methyl, 1-naphthyl, 8- quinolyl, 1- (5-N, N-dimethylamino) -naphthyl , 4- iodophenyl, 2 , 4 , 6-trimethylphenyl , benzyl, 4- nitrophenyl, 2-nitrophenyl , 4-chlorophenyl , and E- sty
- Another exemplary small molecule sulfonamide is a compound of Formula III
- B and D are independently Ar, hydrogen, straight or branched chain alkyl, or C 2 -C 6 straight or branched chain alkenyl, wherein said alkyl or alkenyl is unsubstituted or substituted with C 5 -C 7 cycloalkyl, C 5 -C 7 cycloalkenyl or Ar, and wherein one or two carbon atom(s) of said alkyl or alkenyl may be substituted with one or two heteroatom (s) independently selected from the group consisting of O, S, SO, and S0 2 in chemically reasonable substitution patterns, or
- Q is hydrogen, straight or branched chain alkyl, or C 2 -C 6 straight or branched chain alkenyl ; and T is Ar or C 5 -C 7 cycloalkyl substituted at positions 3 and 4 with one or more substituent (s) independently selected from the group consisting of hydrogen, hydroxy, O- (Ci-C* alkyl), 0- (C 2 -C 4 alkenyl), and carbonyl; provided that both B and D are not hydrogen;
- Ar is selected from the group consisting of phenyl, 1-napthyl, 2 -naphthyl, 2 -furyl, 3 -furyl, 2- thienyl, 3 -thienyl, 2 -pyridyl, 3 -pyridyl, 4 -pyridyl, monocyclic and bicyclic heterocyclic ring systems with individual ring sizes being 5 or 6 which contain in either or both rings a total of 1-4 heteroatoms independently selected from the group consisting of O, N, and S; wherein Ar contains 1-3 substituent (s) independently selected from the group consisting of hydrogen, halo, hydroxy, nitro, trifluoromethyl, trifluoromethoxy, straight or branched chain alkyl, C 2 -C 6 straight or branched chain alkenyl, O- (C x - C 4 straight or branched chain alkyl) , O- (C 2 -C 4 straight or branched chain alkenyl),
- E is C- L -Cg straight or branched chain alkyl, C 2 -C 6 straight or branched chain alkenyl, C 5 -C 7 cycloalkyl, C 5 -C 7 cycloalkenyl substituted with C 1 -C 4 straight or branched chain alkyl or C 2 -C 4 straight or branched chain alkenyl, (C 2 -C 4 alkyl or C 2 -C 4 alkenyl) -Ar, or Ar; and m is 0 to 3.
- a further exemplary small molecule sulfonamide is a compound of Formula IV
- B and D are independently Ar, hydrogen, C ⁇ C g straight or branched chain alkyl, or C 2 -C 6 straight or branched chain alkenyl, wherein said alkyl or alkenyl is unsubstituted or substituted with C 5 -C 7 cycloalkyl, C 5 -C 7 cycloalkenyl, or Ar, and wherein one or two carbon atom(s) of said alkyl or alkenyl may be substituted with one or two heteroatom (s) independently selected from the group consisting of 0, S, SO, and S0 2 in chemically reasonable substitution patterns, or
- Q is hydrogen, straight or branched chain alkyl, or C 2 -C 6 straight or branched chain alkenyl
- T is Ar or C 5 -C 7 cycloalkyl substituted at positions 3 and 4 with one or more substituent (s) independently selected from the group consisting of hydrogen, hydroxy,
- Ar is selected from the group consisting of phenyl, 1-napthyl, 2 -naphthyl, 2 -furyl, 3 -furyl, 2- thienyl, 3 -thienyl, 2 -pyridyl, 3 -pyridyl, 4 -pyridyl, monocyclic and bicyclic heterocyclic ring systems with individual ring sizes being 5 or 6 which contain in either or both rings a total of 1-4 heteroatoms independently selected from the group consisting of O, N, and S; wherein Ar contains 1-3 substituent (s) independently selected from the group consisting of hydrogen, halo, hydroxy, nitro, trifluoromethyl, trifluoromethoxy, straight or branched chain alkyl, C 2 -C 6 straight or branched chain alkenyl, O- (C x - C 4 straight or branched chain alkyl) , 0- (C 2 -C 4 straight or branched chain alkenyl),
- E is straight or branched chain alkyl, C 2 -C 6 straight or branched chain alkenyl, C 5 -C 7 cycloalkyl, C 5 -C 7 cycloalkenyl substituted with C 1 -C 4 straight or branched chain alkyl or C 2 -C 4 straight or branched chain alkenyl, (C 2 -C 4 alkyl or C 2 -C 4 alkenyl) -Ar, or Ar; and m is 0 to 3.
- a further exemplary small molecule sulfonamide is a compound of Formula V
- V is C, N, or S;
- R is either straight or branched chain alkyl, C 2 -C 9 straight or branched chain alkenyl, C 3 -C 9 cycloakyl, C 5 -C 7 cycloalkenyl, or Ar 17 wherein R is either unsubstituted of substituted with one or more substituent (s) independently selected from the group consisting of halo, haloalkyl, carbonyl , carboxy, hydroxy, nitro, trifluoromethyl, C x -C 6 straight or branched chain alkyl, C 2 -C 6 straight or branched chain alkenyl, C 1 -C 4 alkoxy, C 2 -C 4 alken
- Ar x and Ar 2 are independently an alicyclic or aromatic, mono-, bi- or tricyclic, carbo- or heterocyclic ring; wherein the individual ring size is 5-8 members; wherein said heterocyclic ring contains 1-6 heteroatom(s) independently selected from the group consisting of 0, N, and S;
- A, B, D, E, and n are as defined in Formula I above .
- Representative species of Formulas I-V are presented in Table I .
- All the compounds of Formulas I-V possess asymmetric centers and thus can be produced as mixtures of stereoisomers or as individual R- and S- stereoisomers .
- the individual stereoisomers may be obtained by using an optically active starting material, by resolving a racemic or non-racemic mixture of an intermediate at some appropriate stage of the synthesis, or by resolving the compounds of Formulas I-V.
- the compounds of Formulas I-V encompass individual stereoisomers as well as mixtures (racemic and non-racemic) of stereoisomers.
- S-stereoisomers are used in the pharmeceutical compositions and methods of the present invention. Synthesis of Small Molecule Sulfonamides
- the compounds of Formulas I-V may be readily prepared by standard techniques of organic chemistry, utilizing the general synthetic pathway depicted below. As described by Scheme I, amino acids 1 protected by suitable blocking groups P on the amino acid nitrogen may be reacted with alcohols ROH to generate esters 2. After removal of the protecting group, the free amine 3 may be reacted with various sulfonyl chlorides 4 to provide final products 5 in good to excellent yield.
- the compounds used in the inventive methods and pharmaceutical compositions have an affinity for the FK506 binding protein, particularly FKBP12.
- the inhibition of the prolyl peptidyl cis- trans isomerase activity of FKBP may be measured as an indicator of this affinity.
- the cis- trans isomerization of an alanine-proline bond in a model substrate, N-succinyl-Ala-Ala-Pro-Phe- p-nitroanilide is monitored spectrophotometrically in a chymotrypsin-coupled assay, which releases para- nitroanilide from the trans form of the substrate.
- the inhibition of this reaction caused by the addition of different concentrations of inhibitor is determined, and the data is analyzed as a change in first-order rate constant as a function of inhibitor concentration to yield the apparent K ⁇ values.
- a plastic cuvette In a plastic cuvette are added 950 mL of ice cold assay buffer (25 mM HEPES, pH 7.8, 100 mM NaCl) , 10 mL of FKBP (2.5 mM in 10 mM Tris-Cl pH 7.5, 100 mM NaCl, 1 mM dithiothreitol) , 25 mL of chymotrypsin (50 mg/ml in 1 mM HCl) and 10 mL of test compound at various concentrations in dimethyl sulfoxide.
- ice cold assay buffer 25 mM HEPES, pH 7.8, 100 mM NaCl
- FKBP 2.5 mM in 10 mM Tris-Cl pH 7.5, 100 mM NaCl, 1 mM dithiothreitol
- 25 mL of chymotrypsin 50 mg/ml in 1 mM HCl
- 10 mL of test compound at various concentrations
- the reaction is initiated by the addition of 5 mL of substrate (succinyl-Ala-Phe-Pro-Phe-para-nitroanilide, 5 mg/triL in 2.35 mM LiCl in trifluoroethanol) .
- substrate succinyl-Ala-Phe-Pro-Phe-para-nitroanilide, 5 mg/triL in 2.35 mM LiCl in trifluoroethanol
- the absorbance at 390 nm versus time is monitored for 90 seconds using a spectrophotometer and the rate constants are determined from the absorbance versus time data files.
- the compounds used in the inventive methods and pharmaceutical compositions must readily affect the targeted areas.
- the compounds are preferably administered topically to the skin.
- the compounds can be formulated into suitable ointments containing the compounds suspended or dissolved in, for example, mixtures with one or more of the following: mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene polyoxypropylene compound, emulsifying wax and water.
- the compounds can be formulated into suitable lotions or creams containing the active compound suspended or dissolved in, for example, a mixture of one or more of the following: mineral oil, sorbitan monostearate, polysorbate 60, cetyl ester wax, cetearyl alcohol, 2-octyldodecanol , benzyl alcohol and water.
- Dosage levels on the order of about 0.1 mg to about 10,000 mg of the active ingredient compound are useful in the treatment of the above conditions, with preferred levels of about 0.1 mg to about 1,000 mg.
- the specific dose level for any particular patient will vary depending upon a variety of factors, including the activity of the specific compound employed; the age, body weight, general health, sex and diet of the patient; the time of administration; the rate of excretion; drug combination; the severity of the particular disease being treated; and the form of administration. Typically, in vi tro dosage-effect results provide useful guidance on the proper doses for patient administration. Studies in animal models are also helpful. The considerations for determining the proper dose levels are well known in the art.
- the compounds can be administered with other hair revitalizing agents. Specific dose levels for the other hair revitalizing agents will depend upon the factors previously stated and the effectiveness of the drug combination.
- dicyclohexylcarbodiimide (9.20 g;
- Methyl 1- ( ⁇ -tolylsulfonyl) -2-pipecolinate (2.0 g; 6.72 mmol) was dissolved in ethanol (25 mL) and treated with 20 mL of 1 N lithium hydroxide. The mixture was stirred for 2 hours at room temperature, and then diluted with ethyl acetate (200 mL) and made acidic (pH 2) with 1 N HCL. The organic layer was washed with brine, dried over magnesium sulfate, and concentrated to obtain 1.90 g (100%) of the acid as a white solid. 4 -Phenyl -1 -butyl 1- ( ⁇ -tolylsulfonyl) -2-pipecolinate in
- mice were used to demonstrate the hair revitalizing properties of related neuroimmunophilin FKBP ligands, GPI 1044, GPI 1116, and GPI 1102.
- C57 black 6 mice approximately 7 weeks old, had an area of about 2 inches by 2 inches on their hindquarters shaved to remove all existing hair. Care was taken not to nick or cause abrasion to the underlaying dermal layers.
- the animals were in anagen growth phase, as indicated by the pinkish color of the skin. Referring now to FIGS. 1, 2, 3 and 4, four animals were treated by topical administration with 20% propylene glycol vehicle (FIG.
- FIG. 1 shows that animals treated with vehicle exhibited only a small amount of hair growth in patches or tufts, with less than 3% of the shaved area covered with new growth.
- FIGS. 2, 3 and 4 show that animals treated with the related neuroimmunophilin FKBP ligands, 10 ⁇ M GPI 1044, 10 ⁇ M GPI 1116, and 3 ⁇ M GPI 1102, exhibited dramatic hair growth, covering as much as 50% of the shaved area in some animals.
- FIG. 1 shows that animals treated with vehicle exhibited only a small amount of hair growth in patches or tufts, with less than 3% of the shaved area covered with new growth.
- FIGS. 2, 3 and 4 show that animals treated with the related neuroimmunophilin FKBP ligands, 10 ⁇ M GPI 1044, 10 ⁇ M GPI 1116, and 3 ⁇ M GPI 1102, exhibited dramatic hair growth, covering as much as 50% of the shaved area in some animals.
- FIG. 1 shows that animals
- the animals were in a anagen growth phase when shaved.
- Five animals per group were treated by topical administration with a vehicle, FK506, or a neuroimmunophilin FKBP ligand (GPI 1116 or 1206) at a concentration of one micromole per milliliter to the shaved area.
- the animals were treated three times per week, and hair growth was evaluated 14 days after initiation of treatment. Hair growth was quantitated by the percent of shaved area covered by new hair growth, as scored by a blinded observer, on a scale of 0 (no growth) to five (complete hair regrowth in shaved area) .
- Figure 6 shows that after 14 days, the animals treated with vehicle exhibited the beginning of growth in small tufts. In contrast, animals treated with a neuroimmunophilin FKBP ligand exhibited dramatic hair growth .
- a lotion comprising the following composition may be prepared.
- a lotion comprising the following composition shown may be prepared.
- the resulting mixture is stirred, and purified water is added to the mixture to obtain a transparent liquid lotion.
- the lotion may be applied by spraying once to 4 times per day to a site having marked baldness or alopecia .
- An emulsion may be prepared from A phase and B phase having the following compositions.
- the A phase and the B phase are respectively heated and melted and maintained at 80°c. Both phases are then mixed and cooled under stirring to normal temperature to obtain an emulsion.
- the emulsion may be applied by spraying once to four times per day to a site having marked baldness or alopecia .
- a cream may be prepared from A phase and B phase having the following compositions.
- the A phase is heated and melted, and maintained at 70°c.
- the B phase is added into the A phase and the mixture is stirred to obtain an emulsion.
- the emulsion is then cooled to obtain a cream.
- the cream may be applied once to 4 times per day to a site having marked baldness or alopecia.
- a liquid comprising the following composition may be prepared.
- the liquid may be applied once to 4 times per day to a site having marked baldness or alopecia.
- a shampoo comprising the following composition may be prepared.
- the shampoo may be used on the scalp once or twice per day.
- Example 11 The shampoo may be used on the scalp once or twice per day.
- a patient is suffering from alopecia senilis.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient. Increased hair growth is expected to occur following treatment.
- a patient is suffering from male pattern alopecia.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient. Increased hair growth is expected to occur following treatment.
- a patient is suffering from alopecia areata.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient. Increased hair growth is expected to occur following treatment.
- a patient is suffering from hair loss caused by skin lesions.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient . Increased hair growth is expected to occur following treatment.
- a patient is suffering from hair loss caused by tumors.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient. Increased hair growth is expected to occur following treatment.
- a patient is suffering from hair loss caused by a systematic disorder, such as a nutritional disorder or an internal secretion disorder.
- a systematic disorder such as a nutritional disorder or an internal secretion disorder.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient. Increased hair growth is expected to occur following treatment.
- a patient is suffering from hair loss caused by chemotherapy.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient. Increased hair growth is expected to occur following treatment.
- Example 18 A patient is suffering from hair loss caused by radiation.
- a small molecule sulfonamide, or a pharmaceutical composition comprising the same, may be administered to the patient. Increased hair growth is expected to occur following treatment .
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98926183A EP1083876A1 (en) | 1998-06-03 | 1998-06-03 | Small molecule sulfonamide hair growth compositions and uses |
JP2000551746A JP2002516846A (en) | 1998-06-03 | 1998-06-03 | Small molecule sulfonamide hair growth compositions and uses |
CA002334395A CA2334395A1 (en) | 1998-06-03 | 1998-06-03 | Small molecule sulfonamide hair growth compositions and uses |
PCT/US1998/011253 WO1999062490A1 (en) | 1998-06-03 | 1998-06-03 | Small molecule sulfonamide hair growth compositions and uses |
AU78080/98A AU760663B2 (en) | 1998-06-03 | 1998-06-03 | Small molecule sulfonamide hair growth compositions and uses |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1998/011253 WO1999062490A1 (en) | 1998-06-03 | 1998-06-03 | Small molecule sulfonamide hair growth compositions and uses |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999062490A1 true WO1999062490A1 (en) | 1999-12-09 |
Family
ID=22267205
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/011253 WO1999062490A1 (en) | 1998-06-03 | 1998-06-03 | Small molecule sulfonamide hair growth compositions and uses |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1083876A1 (en) |
JP (1) | JP2002516846A (en) |
AU (1) | AU760663B2 (en) |
CA (1) | CA2334395A1 (en) |
WO (1) | WO1999062490A1 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000009103A2 (en) * | 1998-08-14 | 2000-02-24 | Gpi Nil Holdings, Inc. | N-linked sulfonamides of n-heterocyclic carboxylic acids or isosteres for vision and memory disorders |
WO2000009104A2 (en) * | 1998-08-14 | 2000-02-24 | Gpi Nhl Holdings, Inc. | Use of sulfonamides for treating vision and memory disorders |
WO2000018361A2 (en) * | 1998-09-30 | 2000-04-06 | The Procter & Gamble Company | Method of treating hair loss using sulfonamides |
WO2001010837A1 (en) * | 1999-08-05 | 2001-02-15 | The Procter & Gamble Company | Multivalent sulfonamides |
WO2001010838A1 (en) * | 1999-08-05 | 2001-02-15 | The Procter & Gamble Company | Multivalent compounds |
US6300341B1 (en) | 1998-09-30 | 2001-10-09 | The Procter & Gamble Co. | 2-substituted heterocyclic sulfonamides |
US6307049B1 (en) | 1998-09-30 | 2001-10-23 | The Procter & Gamble Co. | Heterocyclic 2-substituted ketoamides |
US20170014327A1 (en) * | 2014-02-27 | 2017-01-19 | Greenever | Composition for promoting hair restoration and/or hair growth containing sulfa agent and chitosan agent |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2505114A1 (en) * | 1975-02-07 | 1976-08-19 | Koehler Valentin | Scalp-care compsns contg. B gp. vitamins - and sulphanilamides, sulphonamides etc., reducing loss of hair, itching, dandruff and greying |
WO1988000040A1 (en) * | 1986-07-02 | 1988-01-14 | American Health Products Corporation | Topical hair growing composition and kit |
EP0443983A1 (en) * | 1990-02-19 | 1991-08-28 | Ciba-Geigy Ag | Acyl compounds |
WO1993014072A1 (en) * | 1992-01-07 | 1993-07-22 | British Bio-Technology Limited | Amino acid derivatives as paf-receptor antagonists |
WO1998022432A1 (en) * | 1996-11-18 | 1998-05-28 | Yamanouchi Pharmaceutical Co., Ltd. | Novel acylamino-substituted acylanilide derivatives or pharmaceutical composition comprising the same |
-
1998
- 1998-06-03 WO PCT/US1998/011253 patent/WO1999062490A1/en not_active Application Discontinuation
- 1998-06-03 CA CA002334395A patent/CA2334395A1/en not_active Abandoned
- 1998-06-03 JP JP2000551746A patent/JP2002516846A/en active Pending
- 1998-06-03 EP EP98926183A patent/EP1083876A1/en not_active Ceased
- 1998-06-03 AU AU78080/98A patent/AU760663B2/en not_active Ceased
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2505114A1 (en) * | 1975-02-07 | 1976-08-19 | Koehler Valentin | Scalp-care compsns contg. B gp. vitamins - and sulphanilamides, sulphonamides etc., reducing loss of hair, itching, dandruff and greying |
WO1988000040A1 (en) * | 1986-07-02 | 1988-01-14 | American Health Products Corporation | Topical hair growing composition and kit |
EP0443983A1 (en) * | 1990-02-19 | 1991-08-28 | Ciba-Geigy Ag | Acyl compounds |
WO1993014072A1 (en) * | 1992-01-07 | 1993-07-22 | British Bio-Technology Limited | Amino acid derivatives as paf-receptor antagonists |
WO1998022432A1 (en) * | 1996-11-18 | 1998-05-28 | Yamanouchi Pharmaceutical Co., Ltd. | Novel acylamino-substituted acylanilide derivatives or pharmaceutical composition comprising the same |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000009103A2 (en) * | 1998-08-14 | 2000-02-24 | Gpi Nil Holdings, Inc. | N-linked sulfonamides of n-heterocyclic carboxylic acids or isosteres for vision and memory disorders |
WO2000009104A2 (en) * | 1998-08-14 | 2000-02-24 | Gpi Nhl Holdings, Inc. | Use of sulfonamides for treating vision and memory disorders |
WO2000009103A3 (en) * | 1998-08-14 | 2000-11-16 | Guilford Pharm Inc | N-linked sulfonamides of n-heterocyclic carboxylic acids or isosteres for vision and memory disorders |
WO2000009104A3 (en) * | 1998-08-14 | 2000-12-07 | Guilford Pharm Inc | Use of sulfonamides for treating vision and memory disorders |
WO2000018361A2 (en) * | 1998-09-30 | 2000-04-06 | The Procter & Gamble Company | Method of treating hair loss using sulfonamides |
WO2000018361A3 (en) * | 1998-09-30 | 2000-05-25 | Procter & Gamble | Method of treating hair loss using sulfonamides |
US6300341B1 (en) | 1998-09-30 | 2001-10-09 | The Procter & Gamble Co. | 2-substituted heterocyclic sulfonamides |
US6307049B1 (en) | 1998-09-30 | 2001-10-23 | The Procter & Gamble Co. | Heterocyclic 2-substituted ketoamides |
WO2001010837A1 (en) * | 1999-08-05 | 2001-02-15 | The Procter & Gamble Company | Multivalent sulfonamides |
WO2001010838A1 (en) * | 1999-08-05 | 2001-02-15 | The Procter & Gamble Company | Multivalent compounds |
US20170014327A1 (en) * | 2014-02-27 | 2017-01-19 | Greenever | Composition for promoting hair restoration and/or hair growth containing sulfa agent and chitosan agent |
Also Published As
Publication number | Publication date |
---|---|
CA2334395A1 (en) | 1999-12-09 |
JP2002516846A (en) | 2002-06-11 |
AU760663B2 (en) | 2003-05-22 |
AU7808098A (en) | 1999-12-20 |
EP1083876A1 (en) | 2001-03-21 |
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