WO1999059994A1

WO1999059994A1 - Retroviral protease inhibiting compounds

Info

Publication number: WO1999059994A1
Application number: PCT/US1999/010130
Authority: WO
Inventors: Xiaoqi Chen; Dale J. Kempf; Daniel W. Norbeck; Fariborz Mohammadi
Original assignee: Abbott Laboratories
Priority date: 1998-05-15
Filing date: 1999-05-07
Publication date: 1999-11-25
Also published as: JP2002515501A; CA2331756A1; EP1077977A1

Abstract

The present invention discloses novel compounds, compositions, and methods for inhibiting retroviral proteases and in particular for inhibiting human immunodeficiency virus (HIV) protease. The present invention also relates to compositions and methods for treating a retroviral infection and in particular a HIV infection, and to processes for making such compounds and synthetic intermediates employed in these processes.

Description

RETROVIRAL PROTEASE INHIBITING COMPOUNDS

Technical Field

The present invention relates to novel compounds compositions and methods for inhibiting retroviral proteases and in particular for inhibiting human immunodeficiency virus (HIV) protease. The present invention also relates to compositions and methods for treating a retroviral infection and in particular an HIV infection, and to processes for making such compounds and synthetic intermediates employed in these processes.

Background of the Invention

Retroviruses are those viruses which utilize a bonucleic acid (RNA) intermediate and a RNA-dependent deoxyribonucleic acid (DNA) polymerase, reverse transcriptase, during their life cycle. Retroviruses include, but are not limited to, the RNA viruses of the Retroviridae family, and also the DNA viruses of the Hepadnavirus and Caulimovirus families. Retroviruses cause a variety of disease states in man, animals and plants. Some of the more important retroviruses from a pathological standpoint include human immunodeficiency viruses (HIV-1 and HIV-2), which cause acquired immune deficiency syndrome (AIDS) in man, hepatitis B virus, which causes hepatitis and hepatic carcinomas in man, human T-cell lymphotrophic viruses I, II, IV and V, which cause human acute cell leukemia, and bovine and feline leukemia viruses which cause leukemia in domestic animals.

Proteases are enzymes which cleave proteins at specific peptide bonds. Many biological functions are controlled or mediated by proteases and their complementary protease inhibitors. For example, the protease renin cleaves the peptide angiotensinogen to produce the peptide angiotensin I. Angiotensin I is further cleaved by the protease angiotensin converting enzyme (ACE) to form the hypotensive peptide angiotensin II. Inhibitors of renin and ACE are known to reduce high blood pressure in vivo. An inhibitor of a retroviral protease will provide a therapeutic agent for diseases caused by the retrovirus.

The genomes of retroviruses encode a protease that is responsible for the proteolytic processing of one or more polyprotein precursors such as the QQ\ and gag gene products. See Wellink, Arch. Virol. ___ 1 (1988). Retroviral proteases most commonly process the gag precursor into core proteins, and also process the p_gj precursor into reverse transciptase and retroviral protease. In addition, retroviral proteases are sequence specific. See Pearl, Nature 328 482 (1987).

The correct processing of the precursor polyproteins by the retroviral protease is necessary for the assembly of infectious virions. It has been shown that in vitro mutagenesis that produces protease-defective virus leads to the production of immature core forms which lack infectivity. See Crawford, J. Virol. 5_3_ 899 (1985); Katoh, et al., Virology 145. 280 (1985). Therefore, retroviral protease inhibition provides an attractive target for antiviral therapy. See Mitsuya, Nature _\__ 775 (1987).

Current treatments for viral diseases usually involve administration of compounds that inhibit viral DNA synthesis. Current treatments for AIDS involve administration of compounds such as 3'-azido-3'-deoxythymidine (AZT), 2',3'-dideoxycytidine (DDC) and 2',3'-dideoxyinosine (DDI) and compounds which treat the opportunistic infections caused by the immunosuppression resulting from HIV infection. None of the current AIDS treatments have proven to be totally effective in treating and/or reversing the disease. In addition, many of the compounds currently used to treat AIDS cause adverse side effects including low platelet count, renal toxicity and bone marrow cytopenia.

Recently the HIV protease inhibitors ritonavir, saquinavir, nelfinavir, and indinavir have been approved in the U.S. for treatment of HIV infections. However, there is a continuing need for improved HIV protease inhibitors.

Summary of the Invention

The present invention comprises retroviral protease inhibiting compounds having formula I.

wherein R¹ is a thiazolyl group having the formula

and R² is a group having the formula:

wherein R⁴ group is -WR⁵.

The R³ group is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl, and Y is CH or N. W is selected from the group consisting of -0-, -S-, or -(CH2)rr. where n is from 0 to 6, with the proviso that when W is O, or S then Y is CH. R⁵ is selected from the group consisting of alkyl, and aryl. Optionally, R⁴ and the ring to which it is attached, taken together can form a bicyclic group having the formula:

The R⁶ group is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl,or (heteroaryl)alkyl, Z is -0-, -S-, -CH2- or -N(R⁷)- ; and R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

The present invention also comprises retroviral protease inhibiting compounds having formula M:

I I wherein R¹ is a thiazolyl group having the formula

and R² is a group having the formula:

N^R⁸ R wherein X is -C(O)- or -S(0)2- and R⁸ is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

R³ is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl and R⁹ is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl. The Z group is -0-, -S-, -CH - or -N(R⁷)-, and R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

The alkyl, aryl, heterocyclic, and heteroaryl groups in the compounds of the invention can be optionally substituted with from 1 to 5 substituents and preferrably from 1 to 3 substituents. The substituents are selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. The invention also includes pharmaceutically acceptable salts, esters or prodrugs of compounds I and M.

Detailed Description of the Invention

All patents, patent applications, and literature references cited in the specification are hereby incorporated by reference in their entirety. In the case of inconsistencies, the present disclosure, including definitions, will prevail.

The present invention comprises retroviral protease inhibiting compounds having formula I:

wherein R¹ is a thiazolyl group having the formula

and R² is a group having the formula:

wherein R⁴ group is -WR⁵.

The R³ group is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl, and Y is CH or N. W is selected from the group consisting of -0-, -S-, or -(CH2)rr, where n is from 0 to

6, with the proviso that when W is O, or S then Y is CH. R⁵ is selected from the group consisting of alkyl, and aryl. Optionally, R⁴ and the ring to which it is attached, taken together can form a bicyclic group having the formula:

with the proviso that when W is O, or S then Y is CH. The R⁶ group is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl, Z is -0-, -S-, -CH2- or -N(R⁷)-; and R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

I I wherein R¹ is a thiazolyl group having the formula:

^R3>^S ^

and R² is a group having the formula:

N"^X R⁸

R⁹ wherein X is -C(O)- or -S(0)2- and R⁸ is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

R³ is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl and R⁹ is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, heteroaryl, or (heteroaryl)alkyl. The Z group is -0-, -S-, -CH2- or -N(R⁷)-, and R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

The alkyl, aryl, heteroaryl, and heterocyclic groups of the compounds of the invention, having formula I or II, can optionally be substituted with from 1 to 5 substituents and preferrably from 1 to 3 substituents. The substituents are selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. The invention also includes pharmaceutically acceptable salts, esters or prodrugs of compounds i and M.

In the compounds of the invention, a preferred R³ group is alkyl or cycloalkyl. More preferred are compounds where R³ is alkyl selected from the group consisting of methyl, ethyl, or propyl or cycloalkyl selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. The most preferred R³ group is isopropyl. The preferred Z groups are -0-, or -N(R⁷)-. When Z is -N(R⁷)- a preferred R⁷ is methyl.

In compounds having formula I, and R² is a group having the formula:

wherein Y is CH or N and R⁴ is -W-R⁵, the preferred R⁵ group is aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl. In compounds where Y is nitrogen, W is -(CH2)n-. R⁵ is alkyl or aryl and n is from 0 to 6.

In compounds where Y is CH, a preferred W is -0-, and preferably, R⁵ is alkyl, or aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl.

The alkyl, aryl, heterocyclic, and heteroaryl groups can be substituted with from 1 to 5 substituents and preferrably from 1 to 3 substituents.

Examples of substituents, for the alkyl, aryl, heterocyclic, and heteroaryl groups, are selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. Preferred substituents are fluorine, hydroxy, alkoxy, or alkylthio groups. The preferred alkoxy is methoxy. The preferred halogen is fluorine in a preferred embodiment the alkyl or aryl groups can be substituted with one to three groups. The preferred substituents are hydroxy, methoxy or fluorine. The preferred aryl groups include phenyl, such as, for example, methylenedioxyphenyl, and heteroaryl such as, for example, furanyl, thienyl, benzothienyl, thiazolyl and the like. A preferred heteroaryl group is thiazolyl. In a preferred compound n is zero, and R⁵ is methyl substituted with a thiazolyl group.

The R⁶ group is selected from the group consisting of alkyl, hydroxyalkyl, and cycloalkyl. Preferably R⁶ is lower alkyl group such as, for example, methyl, ethyl, propyl, butyl and the like. A preferred R⁶ group is tert-butyl or hydroxy- butyl.

The R⁸ group is selected from the group consisting of alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl,or (heteroaryl)alkyl. A preferred R⁸ group is (alkyl)amino, such as, for example, (ferfbutyl)amino.

The preferred R⁹ group is a lower alkyl group such as, for example propyl, butyl, pentyl and the like. More preferred R⁹ groups are isopropyl, tert- butyl, isobutyl, 3-methyl-1 -butyl, and the like. Most preferred is the /^'so-butyl group.

In a preferred compound of the invention X is -S(0)2-, R⁸ is aryl selected from the group consisting of phenyl, and heteroaryl and R⁹ is iso-butyl.

In the compounds of the invention, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds. As used herein, the term "stable compound" refers to a compound that is sufficiently stable to survive isolation to a useful degree of purity from a reaction mixture and formulation into a therapeutic dosage form suitable for administration.

Preferred compounds of the invention are selected from the group consisting of:

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-4-(l,3-benzodioxol-5-ylmethyl)-2-[[(l,l-dimethyl- ethyl)amino]carbonyl]-l -piperazinyl]-2-hydroxy-l -(pheny lmethyl)- propyl] amino] carbonyl]-2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-l- piperazinyl]-2-hydroxy-l - (pheny lmethyl)propyl] amino] carbonyl] -2- methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-(lS)-l-[[[(lS,2R)-3-[(2S)- 2-[[(l,l-dimethylethyl)amino]carbonyl]-4-[(4-fluorophenyl)methyl]- l-piperazinyl]-2-hydroxy-l -(pheny lmethyl)propyl] amino] carbonyl] - 2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-(5-thienylmethyl)- l-piperazinyl]-2-hydroxy-l-(phenylmethyl)propyl] amino] carbonyl] - 2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-[4-(3-hy- droxyphenyl)methyl]-l -piperazinyl] -2 -hydroxy -1 - (pheny lmethyl) - propyl] amino] carbonyl] -2-methylpropyl]carbamate; 2-(l-meth lethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-(3- pyridinylmethyl)-l -piperazinyl]-2-hydroxy-l -(pheny lmethy 1)- propyl] amino] carbonyl ]-2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-(4- py ridi ny lmethy 1)-1 -piper azinyl] -2 -hy droxy-1 - (pheny lmethyl)- propyl] amino] carbonyl] -2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-[(4-hy- droxyphenyl)methyl]-l -piper azinyl] -2 -hydroxy- 1 -(phenylmethyl) - propyl] amino] carbonyl] -2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-(lS)-l-[[[(lS,2R)-3-[(2S)- 4-(lH-benzimidiazol-2-ylmethyl)-2-[[(l,l -dimethyl - ethyl)amino]carbonyl]-l -piperazinyl]-2-hydroxy-l -(phenylmethyl) - propyl] amino] carbonyl] -2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-(2- quin olinylmethyl)-l -piperazinyl]-2-hy droxy-1 -(pheny lmethy 1) - propyl] amino]carbonyl]-2-methylpropyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl [(lS)-l-[[[(lS,2R)-3-[(2S)- 4-[(3,4-dimethoxylphenyl)methyl]-2-[[(l,l-dimethyl- ethyl)amino] carbonyl] -1-piperazinyl] -2-hydroxy-l -(phenylmethyl )- propyl] amino] carbonyl ]-2-methylpropyl]carbamate;

N'-[(lS)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l -dimethyl- ethyl )amino] carbonyl] -4-(5-thiazolylmethyl)- 1-piperazinyl] -2-hydroxy-l -(phenylmethyl)propyl] amino] carbonyl] -2-methylpropyl]-N - methyl -N-[2-(l-methylethyl)-4-thiazolylmethyl] urea;

N'-[(lS)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l-dimethyl- ethyl)amino]carbonyl]-4-(phenylmethyl)-l -piperazinyl]-2-hydroxy- 1 -(pheny lmethy l)propyl] ami no] carbonyl ] -2 -methylpropyl] -N - methyl -N-[2-(l-methylethyl)-4-thiazolylmethyl] urea;

N'-[(lS)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l-dimethyl- ethyl)amino]carbonyl]-4-[(4-hydroxy-3-methoxyphenyl)methyl]-l- piperazinyl] -2-hydroxy-l -(phenylmethyl)propyl] amino] carbonyl] -2- methy lpropyl]-N -methyl -N-[2-(l -methy lethyl) -4- thiazoly lmethyl ]- urea;

2-methyl-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3-[(2S)-4-(l,3- benzodioxol -5 -ylmethyl) -2- [[(l,l-dimethylethyl)amino] carbonyl]-!- piperazinyl] -2-hydroxy-l - (pheny lmethy l)propyl] amino] carbonyl] -2 - methylpropyl]carbamate;

2-methyl-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3-[(2S)-4-[(3,4- dimethoxylphenyl)methyl]-2-[[(l , 1 -dimethylethyl )amino] car bony 1]- 1-piperazinyl] -2-hydroxy-l -(pheny lmethy l)propyl] amino] carbonyl] - 2-methylpropyl]carbamate;

2-methyl-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l- dimethylethyl) amino] carbonyl] -4- [(4-fluorophenyl)methyl]- 1-piperazinyl] -2-hydroxy-l -(phenylmethyl)propyl] amino] carbonyl] -2- methylpropyl]carbamate;

2-ethyl-4-thiazolylmethyl-(lS)-l-[[[(lS,2R)-3-[(2S)-4-[(3,4-di- methoxylphenyl)methyl]-2-[[(l ,1 -dimethy lethy 1) amino] carbonyl]- 1 - piperazinyl] -2-hydroxy-l -(pheny line thyl)propyl] amino] carbonyl] -2- methylpropyl]carbamate;

2-(l-methylethyl)-5-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(l.l-dimethyl- ethyl)amino] carbonyl] -1 -piperazinyl] -2-hydroxy-l -(pheny lmethyl)- propyl] amino] carbonyl]-2-methylpropyl]carbamate;

2-ethyl-5-thiazolylmethyl-(lS)-l-[[[(lS,2R)-3-[(2S)-4-[(3,4-di- methoxylphenyl)methyl]-2-[[(l , 1 -dimethylethyl) amino] carbonyl] -1 - piperazinyl] -2-hydroxy-l -(pheny lmethy l)propyl] amino] carbonyl] -2- methylpropyl]carbamate;

2-methyl-5-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3-[(2S)-4-[(3,4- dimethoxylphenyl)methyl]-2-[[(l , 1 -dimethy lethy l)amino] carbonyl] - 1 -piperazinyl] -2-hydroxy-l -(pheny lmethyl)propyl] amino] carbonyl] - 2-methylpropyl]carbamate;

N'-[(lR)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l-dimethyl- ethyl)amino] carbonyl] -4-(5-thiazolylmethyl)- 1-piperazinyl] -2-hydroxy-l -(phenylmethyl)propyl] amino] carbonyl ]-2-methylpropyl]-N- methyl-N- (5 -thiazoly lmethy l)urea;

N'-[(lS)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l-dimethyl- ethyl)amino] carbonyl] -4-(5-thiazolylmethyl)- 1-piperazinyl] -2-hydroxy-l -(pheny lmethy l)propyl] amino] carbonyl] -2-methylpropyl]-N- methyl-N -(5 -thiazoly lmethy l)urea;

5-thiazolylmethyl-L(lS)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l-dimethyl- ethy l)ami no] carbonyl] -4-(pheny lmethyl) -1-piperazinyl] -2-hydrox y- 1 -(pheny lmethy l)propyl] amino] carbonyl] -2-methylpropyl] carbam- ate;

5-thiazolylmethyl-(lS)-l-[[[(lS,2R)-3-[(2S)-2-[[(l,l-dimethyl- ethy l)amino] carbonyl] -4-(5-th iazolylmethyl)- 1-piperazinyl] -2-hy- droxy-1 -(pheny lmethy l)propyl] amino] carbonyl] -2 -methylpropyl]- carbamate;

N'-[(lS)-l-[[[(lS,2R)-3-[[[(l,l -dimethylethyl) amino] carbonyl] (2-methy lethy l)propylamino] -2-hydroxy-l - (pheny lmethy l)propyl] amino] car bony 1] -2 -methylpropyl] -N- methyl - N - [2 -( 1 -methy lethy 1) -4 -thiazoly lmethyl] urea;

2-(l-methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [[[(1 , 1 -dimethy lethy l)amino] carbonyl] (2-methy lethy l)propylamino] - 2-hydr oxy-1 -(phenylmethyl )pr op yl] amino] carbon yl] -2 -me thy 1- propyl]carbamate;

2-(l-methylethyl)-4-thiazolylmethyl-(lS)-l-[[[(lS,2R)-3-[[(4- aminophenyl )sulfonyl]( 1 -methy lethy 1) amino] -2-hydroxy-l -(phenyl - methy l)propyl] amino] carbonyl] -2 -methy lpropyl]carbamate;

N'-[(lS)-l-[[[(lS,2R)-3-[[(4-aminophenyl)sulfonyl](l-methyl- ethy l)amino] -2-hydroxy-l -(pheny lmethy l)propyl] amino]carbonyl] - 2-methylpropyl] -N -methy 1-N- [2- (1 -methy lethy l)-4-thiazolyl- methyl]urea;

2-(l-methylethyl)-5-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(3 S)-3-[[( 1,1 -dimethylethyl)amino] carbonyl] (4aα,8aα)octahy dro-2- isoquinolinyl] -2-hydroxy-l -(pheny lmethyl )propyl] amino] carbonyl] - 2-methylpropyl] carbamate;

N'-[(lS)-l-[[[(lS,2R)-3-[(3S)-3-[[(l,l-dimethyl- ethy l)amino] carbonyl] (4aα, 8 aα)oc tahy dro-2-isoquinoliny 1] -2-hydroxy-l -(pheny lmethy l)propyl] amino] carbonyl] -2-methy lpropyl]-N methy 1-N- [2 -(1 -methy lethy 1) -5 -thiazoly lmethyl] urea,

N'-[(lS)-l-[[[(lS,2R)-3-[(2S,4R)-2-[[(l,l-dimethyl- ethyl)amino] carbonyl] -4-(5-thiazolylmethoxy)-l -piperidinyl] -2 -hydroxy- 1 -(pheny lmethy l)propyl] amino] carbonyl] -2-methy lpropyl]-N methyl -N-[2-(l -methy lethy 1) -4 -thiazoly lmethyl] urea;

2-(l-methylethyl)-4-thiazolylmethyl [(lS)-l-[[[(lS,2R)-3- [(2S,4R)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-(5- thiazolylmethoxy)-l -piperidinyl] -2-hydroxy-l - (phenylmethyl)propyl] amino] carbonyl] -2-methylpropyl] carbamate;

S-[2-(l-methylethyl)-4-thiazolylmethyl] [(lS)-l-[[[(lS,2R)-3- [(2S)-4-(l,3-benzodioxol-5-ylmethyl)-2-[[(l,l- dimethylethyl)amino] carbonyl] -1-piperazinyl] -2-hydroxy-l - (pheny lmethyl)propyl] amino] carbonyl] -2-methylpropyl] - carbamothioate; and

4-( 1,3 -benzodioxol -5 -y lmethy l)-N-( 1,1 -dimethylethyl)- 1 - [(2R,3S)-3-[[(2S)-2-[[3-[2-(l-methylethyl)-4-thiazolyl]-l- ox opropyl] amino] -3 -methyl- 1 - ox obutyl] amino] -2 -hydroxy -4- phenylbutyl]-2-piperazinecarboxamide; or a pharmaceutically acceptable salt, ester or prodrug thereof.

Most preferred compounds of the invention are selected from the group consisting of:

2-(l-Methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-4-(l,3-beπzodioxol-5-ylmethyl)-2-[[( 1,1 -dimethylethyl )amino]car bony 1]- 1-piperazinyl] -2-hydroxy-l -(phenylmethyl)- propyl]amino]carbonyl] -2-methylpropyl] carbamate;

2-(l-Methylethyl)-4-thiazolylmethyl-(lS)-l-[[[(lS,2R)-3-[[(4- aminophenyl)sulfonyl](l -methy lethy 1) ami no] -2-hydroxy-l - (pheny lmethyl )proρyl] amino] carbonyl] -2-methylpropyl] carbamate;

2-(l-Methylethyl)-4-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(2S)-2-[[(l,l-dimethylethyl)amino]carbonyl]-4-(5-thienylmethyl)- 1 -piperazinyl] -2-hydroxy-l -(pheny lmethyl)propyl] amino] carbonyl] - 2-methylpropyl] carbamate;

2-(l-Methylethyl)-4-thiazolylmethyl [(lS)-l-[[[(lS,2R)-3-[(2S)- 4-[(3,4-dimethoxylphenyl)methyl]-2-[[(l,l-dimethyl- ethyl)amino] carbonyl] -1-piperazinyl] -2-hydroxy-l - (pheny lmethy 1 )- propyl] amino] carbonyl] -2-methylpropyl] carbamate;

N'-[(lS)-l-[[[(lS,2R)-3-[(2S,4R)-2-[[(l,l- Dimethylethyl)amino] carbonyl] -4-(5 -thiazolylmethoxy)- 1 - piperidinyl] -2-hydroxy-l -(phenyl methyl)propyl] amino] carbonyl] -2- methylpropyl]-N-methyl-N-[2-(l-methylethyl)-4~ thiazolylmethyl]urea; and

2-(l-Methylethyl)-5-thiazolylmethyl-[(lS)-l-[[[(lS,2R)-3- [(3 S)-3-[[( 1,1 -dimethy lethy l)amino]carbonyl] (4aα,8aα)octahy dro-2- isoquinolinyl] -2-hydroxy-l -(phenylmethyl )propyl] amino] carbonyl ]- 2-methylpropyl] carbamate; or a pharmaceutically acceptable salt, ester or prodrug thereof.

The term "alkyl" as used herein refers to straight or branched chain alkyl radicals containing from 1 to 12 carbon atoms. The term "lower alkyl" refers to straight or branched chain alkyl radicals containing from 1 to 6 carbon atoms including, but not limited to, methyl, ethyl, n-propyl, /so-propyl, n-butyl, /so-butyl, sec-butyl, f-butyl t?-pentyl, 1-methylbutyl, 2,2-dimethylbutyl, 2-methylpentyl, 2,2- dimethylpropyl, n-hexyl, and the like. The alkyl groups can be unsubstituted or substituted with from one to five substituents independently selected from hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. The alkyl groups can be optionally interrupted by one or more heteroatoms selected from the group consisting of oxygen, nitrogen, sulfur, and phosphorous.

The term "cycloalkyl" as used herein refers to an aliphatic ring system having 3 to 8 carbon atoms including, but not limited to, cyclopropyl, cyclopentyl, cyclohexyl, and the like.

The term "alkoxy" as used herein refers to groups having the formula -OR¹⁰ wherein R¹⁰ is a lower alkyl group.

The term "thioalkyl" as used herein refers to groups having the formula -SR^{1 1} wherein R^{1 1} is a lower alkyl group.

The term "alkylamino" as used herein refers to groups having the formula -NHR¹² wherein R¹² is a lower alkyl group.

The term "dialkylamino" as used herein refers to groups having the formula -N(R¹³)2 wherein each R¹³ is independently a lower alkyl group.

The term "halo or halogen" as used herein refers to F, Cl, Br or I.

The term "(halo)alkyl" as used herein refers to a lower alkyl group in which one or more hydrogen atoms has been replaced with a halogen including, but not limited to, thfluoromethyl, trichloromethyl, difuoromethyl, dichloromethyl, fluoromethyl, chloromethyl, chloroethyl, 2,2-dichloroethyl and the like.

The term "aryl" as used herein refers to a mono- or bicyclic carbocyclic ring system comprising 6 to 12 carbon atoms and having one or two aromatic rings including, but not limited to, phenyl, naphthyl, tetrahydronaphthyl, indanyl, indenyl and the like. Aryl groups can be unsubstituted or substituted with from one to five substituents independently selected from hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen.

The term "(aryl)alkyl" as used herein refers to an aryl group as previously defined, appended to a lower alkyl radical, for example, benzyl and the like.

The term "heterocyclic ring" or "heterocyclic" or "heterocycle" as used herein refers to any 3- or 4-membered ring containing a heteroatom selected from oxygen, nitrogen and sulfur; or a 5-, 6- or 7-membered ring containing one, two or three heteroatoms independently selected from the group consisting of nitrogen, oxygen and sulfur or a 5-membered ring containing 4 nitrogen atoms; and includes a 5-, 6- or 7-membered ring containing one, two or three nitrogen atoms; one oxygen atom; one sulfur atom; one nitrogen and one sulfur atom; one nitrogen and one oxygen atom; two oxygen atoms in non-adjacent positions; one oxygen and one sulfur atom in non-adjacent positions; two sulfur atoms in non-adjacent positions; two sulfur atoms in adjacent positions and one nitrogen atom; two adjacent nitrogen atoms and one sulfur atom; two non- adjacent nitrogen atoms and one sulfur atom; two non-adjacent nitrogen atoms and one oxygen atom. The 5-membered ring has 0-2 double bonds and the 6- and 7-membered rings have 0-3 double bonds. The nitrogen heteroatoms can be optionally quaternized. The term "heterocyclic" also includes bicyclic groups in which any of the above heterocyclic rings is fused to a benzene ring or a cyclohexane ring or another heterocyclic ring (for example, indolyl, quinolyl, isoquinolyl, tetrahydroquinolyl, benzofuryl, bistetrahydrofuranyl or benzothienyl and the like). Heterocyclics include: azetidinyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, pyrazolyl, pyrazolinyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, piperidinyl, homopipe dinyl, pyrazinyl, piperazinyl, pyrimidinyl, py dazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, furyl, thienyl, tetrahydrofuranyl, tetrahydrothienyl, thiazolidinyl, isothiazolyl, t azolyl, tetrazolyl, isoxazolyl, oxadiazolyl, thiadiazolyl, pyrrolyl, pyrimidyl and benzothienyl. Heterocyclics also include compounds of the formula:

wherein X^* is -CH2-, -NH- or -0-, Y^* is -C(O)- or [-C(R")2-]v wherein R" is hydrogen or C1 -C4-alkyl and v is 1 , 2 or 3 and Z^* is -O- or -NH-; such as 1 ,3- benzodioxolyl, 1 ,4-benzodioxanyl and the like.

The term " methylenedioxyphenyl" refers to a substituent having the formula:

Heterocyclic groups can be unsubstituted or substituted with from one to five substituents independently selected from hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. In addition, nitrogen containing heterocycles can be N-protected.

The term "(heterocyclic)alkyl" as used herein refers to a heterocyclic group appended to a lower alkyl radical including, but not limited to, pyrrolidinylmethyl, morpholinylmethyl and the like.

The term "heteroaryl" as used herein refers to an heterocyclic group containing at least one aromatic ring. Examples include groups such as, for example, furanyl, thienyl, benzothienyl, thiazolyl and the like.

The term "(heteroaryl)alkyl" as used herein refers to a heteroaryl group appended to a lower alkyl radical including, but not limited to, thienylmethyl, thienylethyl, furanylmethyl, and the like .

The term "N-protecting group" or "N-protected" as used herein refers to those groups intended to protect the N-terminus of an amino acid or peptide or to protect an amino group against undesirable reactions during synthetic procedures. Commonly used N-protecting groups are disclosed in T.H. Greene and P.G.M. Wuts, Protective Groups in Organic Synthesis. 2nd edition, John Wiley & Sons, New York (1991 ). N-protecting groups comprise acyl groups such as formyl, acetyl, propionyl, pivaloyl, t-butylacetyl, 2-chloroacetyl, 2-bromo- acetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, α-chloro- butyryl, benzoyl, 4-chlorobenzoyl, 4-bromobenzoyl, 4-nitrobenzoyl, and the like; sulfonyl groups such as benzenesulfonyl, p-toluenesulfonyl and the like; carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycar- bonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyl- oxycarbonyl, p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyloxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl, 4-methoxy- benzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxy- benzyloxycarbonyl, 1 -(p-biphenylyl)-l -methylethoxycarbonyl, α,α-dimethyl-

3,5-dimethoxybenzyloxycarbonyl, benzhydryloxycarbonyl, t-butyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycarbonyl, 2,2,2-trichloroethoxycarbonyl, phenoxycarbonyl, 4-nitro-phenoxycarbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl and the like; alkyl groups such as benzyl, t phenylmethyl, benzyloxymethyl and the like; and silyl groups such as thmethylsilyl and the like. Preferred N-protecting groups are formyl, acetyl, benzoyl, pivaloyl, t- butylacetyl, phenylsulfonyl, benzyl, t-butyloxycarbonyl (Boc) and benzyloxycarbonyl (Cbz).

The term "activated carboxylic acid derivative" as used herein refers to acid halides such as acid chlorides, and activated esters including, but not limited to, formic and acetic acid derived anhydrides, anhydrides derived from alkoxycarbonyl halides such as isobutyloxycarbonylchloride and the like, N- hydroxysuccinimide derived esters, N-hydroxyphthalimide derived esters, N- hydroxybenzotriazole derived esters, N-hydroxy-5-norbornene-2,3- dicarboxamide derived esters, 2,4,5-trichlorophenol derived esters, thiophenol derived esters, propylphosphonic acid derived anhydrides and the like.

The term "leaving group" as used herein refers to a group which is easily displaced from the compound, such as, for example, a halide (for example, Cl, Br or I) or a sulfonate (for example, mesylate, tosylate, triflate and the like).

As used herein, the terms "S" and "R" configuration are as defined by the IUPAC 1974 Recommendations for Section E, Fundamental Stereochemistry, Pure Appl. Chem. (1976) 45, 13 - 30.

The compounds of the invention can comprise asymmetrically substituted carbon atoms. As a result, all stereoisomers of the compounds of the invention are meant to be included in the invention, including racemic mixtures, mixtures of diastereomers, as well as single diastereomers of the compounds of the invention.

This invention is intended to encompass compounds having Formula I or Formula II when prepared by synthetic processes or by metabolic processes. Preparation of the compounds of the invention by metabolic processes include those occurring in the human or animal body (in vivo) or processes occurring in vitro.

The reagents required for the synthesis of the compounds of the invention are readily available from a number of commercial sources such as Aldrich Chemical Co. (Milwaukee, WI, USA); Sigma Chemical Co. (St. Louis, MO, USA); and Fluka Chemical Corp. (Ronkonkoma, NY, USA); Alfa Aesar (Ward Hill, MA 01835-9953); Eastman Chemical Company (Rochester, New York 14652-3512); Lancaster Synthesis Inc. (Windham, NH 03087-9977); Spectrum Chemical Manufacturing Corp. (Janssen Chemical) (New Brunswick, NJ 08901 ); Pfaltz and Bauer (Waterbury, CT. 06708). Compounds which are not commercially available can be prepared by employing known methods from the chemical literature.

The compounds of the invention can be prepared as shown in Schemes MIL As outlined in Scheme I, an N-protected (for example, with a benzyloxycarbonyl group) phenylalanylepoxide 2 is coupled with an N- protected (for example, N-Boc) piperazine compound, 1 (using, for example, 1 - (3-dimethylaminopropyl)-3-ethylcarbodiimide (EDAC),1 -hydroxybenzotriazole (HOBT), and an amine, such as, triethyl amine, and the like, in an inert solvent, for example, tetrahydrofuran, methylene chloride, and the like). The protected carboxamide product, 3, is deprotected (for example, by treatment with hydrogen gas and an hydrogenation catalyst). The amine carboxamide 3a, is then coupled with a carboxylic acid, 4, or an activated derivative thereof, using, for example, 1 -(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDAC),1 -hy- droxybenzot azole (HOBT), and triethyl amine in an inert solvent, for example, tetrahydrofuran, methylene chloride, and the like, to provide the N-protected piperazine, 5. The piperazine is N-deprotected, (with for example, trifluoroacetic acid (TFA), and the like) and alkylated (where X¹ is a leaving group) to provide the final product, 6.

3a 4

For piperidyl compounds, where Y is -CH-, the synthesis is outlined in Scheme II, shown below. A mono-protected piperidine carbamate, 7, is alkylated to provide compound 8. The alkylated carbamate is N-deprotected, (with, for example, trifluoroacetic acid, and the like), followed by coupling with the epoxide, 9, to provide carboxamide, 10. The carboxamide is N-deprotected to provide compound 10a. Compound 10a is coupled with a carboxylic acid, 4, or an activated derivative thereof, to provide the product, 11.

SCHEME

The synthesis of compounds of the invention having the -N(R⁹)-X-R⁸ group, is outlined in Scheme ML, Urea, 12, prepared according to the description in J. Med. Chem., 1993, 36, 288-291 , is coupled with a carboxylic acid, 4, or an activated derivative thereof, using, for example, 1 -(3- dimethylaminopropyl)-3-ethylcarbodiimide (EDAC),1 -hydroxybenzot azole (HOBT), and triethyl amine in an inert solvent, for example, tetrahydrofuran, methylene chloride, and the like, to provide the product 13.

SCHEME III

12

13

The following examples will serve to further illustrate the preparation of the novel compounds of the invention.

Example 1 2-(1 -Methylθthyl)-4-thiazolylmethyl-[(1 SV1 -[[[(1 S.2R)-3-[f2S)-4-(1.3-benzodi- oxol-5-ylmethvπ-2-f[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hv- droxy-1 -(phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate. 1A. Benzyl-1 -[⁽1 S.2R)-3-[(2S)-4-(1 .1 -dimethylethyloxy(carbonyl))-2-[[(1 .1 -di- methylethvπamino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 - (phenylmethyl)propyl]carbamate.

The title compound was prepared according to the method described in J. Med. Chem , 1993, 36, 288-291 , starting with 3.6 g (12 mmol) of N-benzyloxycarbonyl-phenylalanylepoxide and 3.4 g (12 mmol) of N-.et -butyl- 4-(terf-butyloxycarbonyl)-piperazine -2(S)-carboxamide, prepared according to the method described in J. Med. Chem., 1994 37, 3443-3451.

1 B. 1 -[(1 S.2R 3-rf2S -π .1 -Dimethylethyloxy⁽carbonyl))-2-[[(1.1 -dimethyl- ethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amine.

A solution of 2.1 g (3.6 mmol) of benzyl-1 -[(1 S,2R)-3-[(2S)-4-(1 ,1 -di- methylethyloxy(carbonyl))-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]- 2-hydroxy-1 -(phenylmethyl)propyl]carbamate in 20 ml of methanol was treated with hydrogen, at greater than 1 atmosphere, in the presence of Pd/C (50 mg; 10%), and stirred for 2 hours. The catalyst was removed via filtration over a celite pad and the solvent was evaporated at reduced pressure to provide the title compound as a yellow oil (yield: 1.6 g; 99%).

1 C. 2-(1 -MethylethylV4-thiazolylmethyl-(1 S)-1 -[[[(1 S.2R)-3-f.2S)-4-f 1 .1 -di- methylethyloxy(carbonvπ)-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]- 2-hvdroxy-1 -(phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate.

A solution of 1.5 g (3.3 mmol) of 1 -[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethyl- ethyloxy(carbonyl))-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hy- droxy-1 -(phenylmethyl)propyl]amine. in 7 ml of tetrahydrofuran (THF) and 7 ml of methylene chloride was prepared and treated with 1.0 g (3.3 mmol) of [(2- isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine, 642 mg (3.3 mmol) of 1 -(3- dimethylaminopropyl)-3-ethylcarbodiimide (EDAC), 45 mg (0.33 mmol) of 1 -hy- droxybenzotriazole (HOBT), and 466 μl (3.3 mmol) of triethyl amine. The solution was stirred at room temperature for 6 hours, and concentrated in vacua. The residue was taken up with ethyl acetate, washed with equal portions of 3 N HCI solution, 10% aqueous NaHCθ3 (20 mL), saturated brine, and dried over MgSO The product was concentrated in vacuo and purified by chromatography on silica gel, using 5% methanoLmethylene chloride, to provide the title compound (yield: 2 .4 g, 98%).

MS: 731 (M+H)+.

¹ H NMR (DMSO-de) δ 1.08 (d, J = 7 Hz, 6H), 2.78 (heptet, J = 7 Hz, 1 H), 9.06 (br s, 1 H), 9.30 (br s, 1 H).

1 D. 2-.1 -Methylethvn-4-thiazolylmethyl-f 1 SV-1 -[[[.1 S.2 -3-|ϊ2SΪ-2-rfM .1 -di- methylethyhamino]carbonyl]-1 -piperazinyll-2-hvdroxy-1 -(phenylm9thv propyl]- amino]carbonyl]-2-methvlpropvl]carbamate.

A solution of 2.4 g (3.2 mmol) of 2-(1 -methylethyl)-4-thiazolylmethyl-(1 S)- 1 -[[[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethylethyloxy(carbonyl))-2-[[(1 ,1 -dimethylethyl)- amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]carbamate in 20 ml of dichloromethane was prepared and treated with 2 ml of trifluoroacetic acid. The reaction was monitored by TLC. After the reaction was completed, the solvent was removed at reduced pressure. The residue was dissolved in methylene chloride, washed with saturated aqueous NaHC03 and saturated brine. The organic layer was dried over Na2Sθ4 and concentrated in vacuo to provide the title compound as a light yellow oil (yield: 2.0 g; 90%).

1 E. 2-(1 -Methylethyl)-4-thiazolylmethyl-[(1 S)-1 -[f[M S.2R)-3-[ 2S)-4-(1 .3-benzo- dioxol-5-ylmethyl)-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hy- droxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

A solution of 514 mg (0.82 mmol) of 2-(1-methylethyl)-4-thiazolylmethyl- (1 S)-1 -[[[(1 S,2R)-3-[(2S)-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]- 2-hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl] carbamate in 5 ml of dimethylformamide (DMF) was prepared and treated with 140 mg (0.8 mmol) of 3,4-dioxomethylenebenzyl chloride followed by 284 μl (1.6 mmol) of N,N-diisopropyl ethyl amine, stirred at room temperature over night, and partitioned between ethyl acetate and saturated sodium bicarbonate solution. The organic layer was washed with brine, dried over sodium sulfate, and concentrated in vacuo. The residue was purified by chromatography on silica gel, using 5% methanoLmethylene chloride, to provide the title compound (yield: 502 mg; 81 %).

MS: 765 (M+H)+. H NMR (CDCI₃) δ 0.67 (d, J = 6.3 Hz, 3H), 0.83 (d, J = 6.3 Hz, 3H), 1.37 (s, 9H), 1.41 (t, J = 7.5, 6H), 2.05 (hexlet, J = 6.0 Hz, 1 H), 2.26 (m, 1 H), 2.41 (m, 1 H), 2.58 (m, 2H), 2.73 (m, 3H), 2.83 (m, 2H), 2.90 (m, 1 H), 3.33 (AB, J = 15.0 Hz, 2H), 3.46 (s, 2H), 3.80 (m, 1 H), 3.93 (m, 1 H), 4.22 (septet, J = 4.5 Hz, 1 H), 5.08 (m, 1 H), 5.17 (d, J = 4.5 Hz, 2H), 5.97 (s, 2H), 6.13 (d, J = 9.0 Hz, 1 H), 6.71 (s, 1 H), 6.75 (s, 2H), 7.12-7.22 (m, 7H), 8.19 (br s, 1 H).

Example 2 2-(1 -Methylethyl)-4-thiazolylmethyl-r SH -[[[⁽1 S.2R)-3-[(2S⁾-2-[[(1.1 -dimethyl- ethvhamino]carbonvl]-4-(phenylmethyl)-1 -piperazinyl]-2-hydroxy-1 -(ph8nyl- methvnpropyl]amino1carbonyl1-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting benzyl bromide in place of 3,4-dioxomethylenebenzyl chloride. MS: 721 (M+H)⁺.

1 H NMR (CDCI3) δ 0.68 (d, J = 6.3 Hz, 3H), 0.84 (d, J = 6.3 Hz, 3H), 1.36 (s, 9H), 1.40 (d, J = 6.3, 6H), 2.04 (hextet, J = 6.0 Hz, 1 H), 2.28 (dt, J = 9.3, 3.3 Hz, 1 H), 2.45 (dt, J = 12.0, 3.0 Hz, 1 H), 2.58 (m, 2H), 2.75 (m, 2H), 2.88 (m, 2H), 3.33 (heptet, J = 6.3 Hz, 1 H), 3.37 (m, 1 H), 3.46 (s, 2H), 3.79 (m, 1 H), 3.85 (dd, J = 8.4, 6.0 Hz, 1 H), 4.22 (septet, J = 4.5 Hz, 1 H), 5.11 (dd, J = 10.8, 7.5 Hz, 1 H), 5.17 (d, J = 3.3 Hz, 2H), 6.13 (d, J = 9.0 Hz, 2H), 7.12-7.33 (m, 11 H), 8.02 (s, 1 H), 8.26 (br s, 1 H).

Example 3 2-ri -Methylethyl)-4-thiazolylmethyl-π S⁾-1 -[[[(1 S.2R)-3-[(2S)-2-[rf1.1 -dimethyl- ethyl)amino]carbonyl]-4-[(4-fluorophenyl)methyl]-1 -piperazinyl]-2-hydroxy-1 - (phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 4-fluorobenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 739 (M+H)+.

1 H NMR (CDCI3) δ 0.67 (d, J = 6.3 Hz, 3H), 0.84 (d, J = 6.3 Hz, 3H), 1.35 (s, 9H), 1.41 (d, J = 7.5, 6H), 2.06 (hextet, J = 6.0 Hz, 1 H), 2.30 (m, 1 H), 2.48 (m, 1 H), 2.59 (m, 1 H), 2.73 (m, 2H), 2.83 (m, 2H), 2.92 (m, 2H), 3.33 (AB, J = 15.0 Hz, 2H), 3.37 (m, 1 H), 3.44 (s, 2H), 3.83 (m, 2H), 4.22 (m, 1 H), 5.09 (m, 1 H), 5.17 (d, J = 4.8 Hz, 2H), 6.19 (d, J = 9.0 Hz, 1 H), 7.03 (d, J = 8.4 Hz, 1 H), 7.12- 7.22 (m, 9H), 8.09 (br s, 1 H).

Example 4 2-(1 -Methylethyl)-4-thiazolylmethyl-[(1 SV1 -[[[(1 S.2R)-3-[(2S -2-[f(1 .1 -dimethyl- ethvπamino1carbonyl]-4-f5-thienylmethvπ-1 -piperazinyl]-2-hvdroxy-1 -fphenyl- methyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 5-(chloromethyl)thiazole in place of 3,4-dioxomethylenebenzyl chloride.

MS: 728 (M+H)+.

1 H NMR (CDCI3) δ 0.68 (d, J = 6.3 Hz, 3H), 0.83 (d, J = 6.3 Hz, 3H), 1.38 (s, 9H), 1.41 (d, J = 7.5, 6H), 2.06 (hextet, J = 6.0 Hz, 1 H), 2.33 (m, 1 H), 2.58 (m, 2H), 2.73 (m, 2H), 2.83 (m, 2H), 2.90 (m, 1 H), 3.33 (heptet, J = 4.5 Hz, 1 H), 3.36 (m, 1 H), 3.73 (d, J = 3.0 Hz, 2H), 3.80 (m, 1 H), 3.83 (dd, J = 7.5, 6.0 Hz, 1 H), 4.22 (septet, J = 4.5 Hz, 1 H), 5.07 (d, J = 7.5 Hz, 1 H), 5.17 (d, J = 4.5 Hz, 2H), 6.15 (d, J = 7.5 Hz, 1 H), 7.12-7.22 (m, 8H), 7.40 (m, 1 H), 7.73 (s, 1 H), 7.79 (s, 1 H), 8.80 (s, 1 H).

Example 5 2-(1 -Methylethyl)-4-thiazolylmethyl-f(1 S)-1 -[[f(1 S.2R⁾-3-[(2S⁾-2-[[(1 .1 -dimethyl- ethyl)amino]carbonyl]-4-[4-(3-hydroxyphenyl)methyl]-1 -piperazinyl]-2-hydroxy- 1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 3-hydroxybenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 737 (M+H)+.

¹ H NMR (CDCI₃) δ 0.67 (d, J = 6.3 Hz, 3H), 0.83 (d, J = 6.3 Hz, 3H), 0.96 (m, 1 H), 1.37 (s, 9H), 1.40 (d, J = 7.5, 6H), 2.03 (hextet, J = 6.0 Hz, 1 H), 2.32 (m, 1 H), 2.45 (m, 1 H), 2.61 (m, 2H), 2.76 (m, 2H), 2.85 (m, 2H), 2.89 (m, 2H), 3.32 (m, 1 H), 3.37 (s, 1 H), 3.42 (m, 1 H), 3.61 (t, J = 8.4 Hz, 1 H), 3.87 (m, 2H), 4.22 (m, 1 H), 4.63 (s, 1 H), 5.16 (s, 2H), 6.36 (m, 1 H), 6.78 (d, J = 7.5 Hz, 2H), 7.12-7.22 (m, 9H), 8.19 (br s, 1 H).

Example 6 2-π -Methvlethvn-4-thiazolvlmethvl-rπ SV1 -frr.1 S.2R -3- (2S)-2-[[(1.1 -dimethyl- ethvnamino1carbonvl]-4-(3-pvridinvlmethvh-1 -piperazinvl]-2-hvdroxv-1 - (phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 3-picolyl chloride hydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 722 (M+H)+.

1 H NMR (CDCI) δ 0.68 (d, J = 6.3 Hz, 3H), 0.83 (d, J = 6.3 Hz, 3H), 1.36

(s, 9H), 1.41 (d, J = 7.5 Hz, 6H), 2.06 (Hextet, J = 4.5 Hz, 1 H), 2.47 (m, 1 H), 2.60 (m, 2H), 2.71 (m, 2H), 2.81 (m, 1 H), 2.83 (m, 2H), 2.92 (m, 1 H), 3.34 (heptet, J = 4.5 Hz, 1 H), 3.35 (m, 1 H), 3.51 (s, 2H), 3.81 (m, 1 H), 3.85 (m, 1 H), 4.22 (m, 1 H), 4.75 (m, 1 H), 5.07 (d, J = 9.0 Hz, 1 H), 5.17 (d, J = 7.8 Hz, 2H), 6.15 (d, J = 7.8 Hz, 1 H), 7.12-7.22 (m, 8H), 7.61 (d, J = 7.8 Hz, 1 H), 7.89 (br s, 1 H), 8.55 (d, J = 3 Hz, 1 H), 8.56 (d, J = 6.0 Hz, 1 H).

Example 7 2-(1 -Methylethvn-4-thiazolylmethvK(1 S)-1 -r[r(1 S.2R)-3-[(2S)-2-[[(1 .1 -dimethyl- ethyl)amino]carbonyl]-4-(4-pyridinylmethy0-1 -piperazinyl]-2-hydroxy-1 - (phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E substituting 4-picolyl chloride hydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 722 (M+H)+.

1 H NMR (CDCI3) δ 0.65 (d, J = 6.3 Hz, 3H), 0.94 (d, J = 6.3 Hz, 3H), 1.37 (s, 9H), 1.41 (d, J = 7.5, 6H), 2.07 (hex let, J = 6.0 Hz, 1 H), 2.37 (m, 1 H), 2.57 (m, 1 H), 2.62 (m, 2H), 2.69 (m, 2H), 2.81 (m, 2H), 2.91 (m, 1 H), 3.32 (m, 1 H), 3.34 (m, 1 H), 3.49 (AB, J = 15.0 Hz, 2H), 3.80 (m, 1 H), 3.85 (m, 1 H), 4.21 (m, 1 H), 4.62 (m, 1 H), 5.06 (d, J = 7.5 Hz, 1 H), 5.14 (m, 1 H), 5.17 (d, J = 4.5 Hz, 2H), 6.14 (d, J = 8.4 Hz, 1 H), 7.12-7.22 (m, 8H), 7.77 (br s, 1 H), 8.08 (d, J = 6.0 Hz, 2H).

Example 8 2-(1 -Methyl9thyl)-4-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-[(2S)-2-[[( 1.1 -dimsthyl- ethvhamino]carbonyl]-4-f(^,4-hvdroxyphenvπmethyl]-1 -piperazinyl]-2-hvdroxy-1 - (phenvlmethvπpropvl1amino]carbonvl]-2-methvlpropvl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 4-hydroxybenzyl chloride hydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 737 (M+H)⁺. H NMR (CDCI3) δ 0.68 (d, J = 6.3 Hz, 3H), 0.83 (d, J = 6.3 Hz, 3H), 1.35 (s, 9H), 1.41 (d, J = 7.5, 6H), 2.03 (hextet, J = 4.5 Hz, 1 H), 2.27 (dt, J = 7.5, 3.0 Hz, 1 H), 2.36 (dd, J = 12.0, 3.0 Hz, 1 H), 2.59 (m, 2H), 2.74 (m, 2H), 2.87 (m, 2H), 2.79 (m, 1 H), 2.92 (m, 1 H), 3.32 (m, 1 H), 3.33 (AB, J = 15.0 Hz, 2H), 3.40 (m, 2H), 3.72 (m, 1 H), 3.78 (dd, J = 8.4, 6.0 Hz, 1 H), 4.22 (septet, J = 4.5 Hz, 1 H), 5.22 (d, J = 4.5 Hz, 2H), 5.23 (m, 1 H), 6.17 (m, 1 H), 6.40 (d, J = 9.0 Hz, 1 H), 6.80 (d, J = 8.7 Hz, 2H), 7.09-7.21 (m, 8H), 8.31 (br s, 1 H). Example 9 2-(1 -Methylethvh-4-thiazolylmethyl-π S)-1 -[[[(1 S.2R)-3-f(2S 4-M H- benzimidiazol-2-ylmethyl)-2-[[f1 .1 -dimethylethvhamino]carbonyl]-1 -piperazinyl]- 2-hydroxy-1 -(phenvlmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 2-(chloromethyl)benzimidazole in place of 3,4-dioxomethylenebenzyl chloride.

MS: 761 (M+H)+. H NMR (CDCI₃) δ 0.62 (d, J = 6.3 Hz, 3H), 0.83 (d, J = 6.3 Hz, 3H), 1.41 (d, J = 7.5, 6H), 1.43 (s, 9H), 2.11 (hextet, J = 4.5 Hz, 1 H), 2.52 (m, 1 H), 2.57 (dd, J = 12.0, 3.0 Hz, 1 H), 2.72 (m, 2H), 2.79 (m, 3H), 3.02 (dd, J = 15.0, 4.5 Hz, 1 H), 3.17 (m, 3H), 3.34 (heptet, J = 4.5 Hz, 1 H), 3.85 (m, 2H), 3.95 (d, J = 9.0 Hz, 1 H), 4.25 (m, 1 H), 4.98 (m, 1 H), 5.13 (s, 2H), 5.17 (m, 1 H), 6.17 (m, 1 H), 7.12-7.28 (m, 11 H), 7.63 (m, 2H).

Example 10 2-f1-Methylethvπ-4-thiazolylmethyl-r(1SV1-rrrf1 S.2R -3-r(2SV2-rrf1.1 -dimethyl- ethyl)amino1carbonyl]-4-f2-quinolinylmethvπ-1 -piperazinyl]-2-hvdroxy-1 - (phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 2-(chloromethyl)quinoline monohydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 772 (M+H)+.

¹ H NMR (CDCI3) δ 0.67 (d, J = 6.3 Hz, 3H), 0.83 (d, J = 6.3 Hz, 3H), 1.37

(s, 9H), 1.40 (d, J = 7.5, 6H), 2.05 (hextet, J = 6.0 Hz, 1 H), 2.50 (m, 1 H), 2.61 (m, 2H), 2.70 (m, 1 H), 2.71 (m, 2H), 2.85 (m, 2H), 2.92 (m, 1 H), 3.32 (m, 1 H), 3.47 (m, 1 H), 3.82 (m, 2H), 3.85 (m, 1 H), 4.22 (septet, J = 4.5 Hz, 1 H), 4.79 (s, 1 H), 5.10 (m, 1 H), 5.17 (d, J = 8.4 Hz, 2H), 6.11 (s, 1 H), 7.12-7.22 (m, 9H), 7.47 (d, J = 6.6 Hz, 1 H), 7.56 (t, J = 7.5 Hz, 1 H), 7.73 (t, J = 8.4 Hz, 1 H), 7.83 (d, J = 8.4 Hz, 1 H), 8.07 (d, J = 7.5 Hz, 1 H), 8.17 (d, J = 8.4 Hz, 1 H).

Example 11 2-(1 -Methylethvh-4-thiazolylmethyl [(1 S)-1 -[[[(1 S.2R)-3-[(2S)-4-[(3.4-di- methoxylphenyl)methyl1-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2- hvdroxv-1 -(phenvlmethvnpropvl]amino]carbonvl]-2-methvlpropvl]carbamate.

The title compound was prepared according to the method described in Example 1 E, substituting 3,4-dimethoxy benzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 781 (M+H)+.

¹ H NMR (CDCI₃) δ 0.68 (d, J = 6.3 Hz, 3H), 0.84 (d, J = 6.3 Hz, 3H), 1.35

(s, 9H), 1.41 (d, J = 6.3, 6H), 2.05 (hextet, J = 6.0 Hz, 1 H), 2.25 (m, 1 H), 2.45 (m, 1 H), 2.58 (m, 2H), 2.74 (m, 3H), 2.84 (m, 2H), 2.90 (m, 1 H), 3.35 (heptet, J = 6.3 Hz, 1 H), 3.40 (AB, J = 15.0 Hz, 2H), 3.81 (m, 1 H), 3.87 (s, 3H), 3.88 (s, 3H), 4.21 (m, 1 H), 5.08 (m, 1 H), 5.17 (d, J = 3.3 Hz, 2H), 6.12 (m, 1 H), 6.26 (s, 1 H), 6.82 (s, 2H), 7.10-7.22 (m, 8H), 8.21 (br s, 1 H).

Example 12 N'-[(1 S;-1 -[[[(1 S.2R)-3-[(2SV2-[f.1 .1 -Dim8thylethyl)amino]carbonyl|-4-⁽5- thiazolylmethyl)-1 -piperazinyl]-2-hvdroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]-N-methyl-N-[2-(1 -methylethyl)-4-thiazolylmethyl]urea.

12A. N'-[(1 S)-1 -[[[(1 S.2R)-3-[(2S⁾-2-[[M .1 -Dimethylethyl)amino]carbonyl]-4- ⁽1 .1 -dimethylethyloxy(carbonym-1 -piperazinvl]-2-hvdroxy-1 -(phenylmethyl)- propyl1amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1 -m9thylethvh-4-thia- zolylmethyl]urea.

A solution of 1.5 g (3.3 mmol) of 1 -[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethyl- ethyloxy(carbonyl))-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hy- droxy-1 -(phenylmethyl)propyl]amine in 7 ml of THF and 7 ml of methylene chloride was prepared and treated with 1.0 g (3.3 mmol) of N-methyl-N-[(((2- isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine, 642 mg (3.3 mmol) of EDAC, 45 mg (0.33 mmol) of HOBT, and 466 μl (3.3 mmol) of triethyl amine.

The solution was stirred at room temperature for 6 hours, and concentrated in vacuo. The residue was taken up with ethyl acetate, washed with equal portions (15 ml) of 3 N HCI solution, 10% aqueous NaHCθ3_, and saturated brine. The organic layer was dried over MgSO-i and concentrated in vacuo. The mixture was purified by chromatography on silica gel using 5% methanoLmethylene chloride to provide the title compound (yield: 2.4 g; 98%).

12B. N'-fM SV1 -rrfM S.2 -3-K2SV2-rrM .1 -Dimethvlethvnaminolcarhonvπ-1 - piperazinyl]-2-hvdroxy-1 - henylmethvBpropyl]amino]carbonyl]-2- methylpropyl]-N-methyl-N-[2-f1 -methylethvh-4-thiazolylmethyl]urea.

A solution of 2.4 g (3.2 mmol) of N'-[(1 S)-1-[[[(1 S,2R)-3-[(2S)-2-[[(1 ,1 - dimethylethyl)amino]carbonyl]-4-(1 ,1 -dimethylethyloxy(carbonyl))-1 -piperazin- yl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]-N- methyl-N-[2-(1 -methylethyl)-4-thiazolylmethyl]urea in 20 ml of dichloromethane, was prepared and treated with 2 ml of trifluoroacetic acid. The reaction was monitored by TLC. After the reaction was completed, the solvent was removed at reduced pressure. The residue was dissolved in methylene chloride, washed with saturated aqueous NaHCθ3, and saturated brine. The organic layer was dried over Na2Sθ4 and concentrated in vacuo to provide the title compound as a light oil (yield: 2.0 g; 90%). The product was used in Example 12C without further purification.

12C. N'-[(1 S)-1 -[[[(1 S.2RK3-[⁽2S⁾-2-rrM .1 -Dimethylethvnamino]carbonyl]-4-(5- thiazolylmethyπ-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]-N-methyl-N-[2-f1 -methylethvπ-4-thiazolylmethyl]urea.

The title compound was prepared according to the method described in Example 1 E, starting with the product, prepared in Example 12B, and substituting 5-thiazolylmethyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 741 (M+H)⁺.

1 H NMR (CDCI₃) δ 0.75 (d, J = 6.3 Hz, 3H), 0.89 (d, J = 6.3 Hz, 3H), 1.33

(d, J = 3.0 Hz, 3H), 1.45 (d, J = 3.0 Hz, 3H), 1.47 (s, 9H), 2.18 (m, 1 H), 2.40 (dt, J = 7.5, 3.0 Hz, 1 H), 2.55 (m, 1 H), 2.62 (m, 4H), 2.73 (dd, J = 15.0, 4.5 Hz, 2H), 2.89 (m, 1 H), 2.95 (s, 3H), 3.22 (heptet, J = 6.0 Hz, 1 H), 3.27 (d, J = 3.0 Hz, 2H), 3.73 (d, J = 3.0 Hz, 2H), 3.87 (m, 1 H), 3.99 (dd, J = 6.3, 6.0 Hz, 1 H), 4.16 (septet, J = 4.5 Hz, 1 H), 4.37 (s, 2H), 4.61 (br s, 1 H), 6.00 (br s, 1 H), 6.39 (d, J = 9.0 Hz, 1 H), 6.97 (s, 1 H), 7.08-7.17 (m, 5H), 7.54 (s, 1 H), 7.72 (s, 1 H), 8.79 (s, 1 H).

Example 13 N'-[(1 S)-1 -[[[(1 S.2RV3-[(2S)-2-fr(1 .1 -Dimethylethyl)amino]carbonyl]-4- (phenylmethyl)-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea.

The title compound was prepared according to the method described in Example 12C, substituting benzyl bromide in place of 5-thiazolylmethyl chloride.

MS: 734 (M+H)+.

¹ H NMR (CDCI3) δ 0.75 (d, J = 6.3 Hz, 3H), 0.88 (d, J = 6.3 Hz, 3H), 1.45 (d, J = 7.5 Hz, 6H), 1.46 (s, 9H), 2.15 (hextet, J = 6.3 Hz, 1 H), 2.32 (dt, J = 9.0, 3.0 Hz, 1 H), 2.51 (dd, J = 8.4, 3.0 Hz, 1 H), 2.59 (dd, J = 8.4, 3.0 Hz, 1 H), 2.70 (m, 2H), 2.78 (m, 2H), 2.95 (s, 3H), 3.24 (heptet, J = 7.5 Hz, 1 H), 3.32 (t, J = 3.0 Hz, 1 H), 3.45 (s, 2H), 3.81 (m, 1 H), 4.01 (dd, J = 7.5, 6.0 Hz, 1 H), 4.17 (septet, J = 4.5 Hz, 1 H), 4.48 (s, 2H), 4.84 (br s, 1 H), 5.89 (m, 1 H), 6.48 (d, J = 9.0 Hz, 1 H), 6.95 (s, 1 H), 7.07-7.18 (m, 5H), 7.25-7.34 (m, 5H), 8.04 (br s, 1 H).

Example 14 N'-rπ SV1-rrr.1 S.2RV3-[r2SV2-rr(1.1-Dimethylethvnamino1carbonyl]-4-r.4-hv- droxy-3-methoxyphenvπmethyl]-1 -piperazinyl]-2-hvdroxy-1 -(phenylmethvπ- propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1 -methylethvπ-4-thia- zolylmethyl]urea. The title compound was prepared according to the method described in Example 12C substituting 3-methoxy-4-hydroxybenzyl chloride in place of 5-thiazolylmethyl chloride.

MS: 780 (M+H)⁺.

1 H NMR (CDCI₃) δ 0.76 (d, J = 6.3 Hz, 3H), 0.88 (d, J = 6.3 Hz, 3H), 1.35

(s, 9H), 1.36 (d, J = 7.5, 6H), 2.17 (hextet, J = 6.0 Hz, 1 H), 2.31 (dt, J = 7.5, 3.0 Hz, 1 H), 2.53 (m, 2H), 2.63 (m, 2H), 2.78 (m, 2H), 2.91 (m, 1 H), 2.96 (s, 3H), 3.26 (q, J = 7.5 Hz, 2H), 3.29 (m, 1 H), 3.38 (AB, J = 15.0 Hz, 2H), 3.83 (m, 1 H), 3.89 (s, 3H), 4.01 (dd, J = 7.5, 6.0 Hz, 1 H), 4.17 (septet, J = 4.5 Hz, 1 H), 4.38 (s, 2H), 4.77 (m, 1 H), 5.58 (s, 1 H), 5.92 (m, 1 H), 6.38 (d, J = 9.0 Hz, 1 H), 6.77 (m, 2H), 6.87 (d, J = 8.4 Hz, 1 H), 6.96 (s, 1 H), 7.12-7.22 (m, 6H), 7.96 (br s, 1 H).

Example 15 2-Methyl-4-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-[(2SV4-(1 .3-benzodioxol-5- ylmethyl)-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 - (phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate. 15A. 2-Methyl-4-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-[(2S)-4-(1 .1 -dimethylethyl- oxy(carbonyl))-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy- 1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method of Example 1 C substituting [(2-methyl-4-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine. This was followed by treatment of the product with trifluoroacetic acid according to the method of Example 1 D

15B. 2-Methyl-4-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-f (2SV4-.1 .3-benzodioxol- 5-ylmethyl)-2-[[(1.1 -dimethvlethvhamino]carbonyl]-1 -piperazinyl]-2-hvdroxy-1 - (phenvlmethvnpropvl1amino1carbonvl]-2-methvlpropvl]carbamate.

A solution of 98 mg (0.16 mmol) of 2-methyl-4-thiazolylmethyl-[(1 S)-1 - [[[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethylethyloxy(carbonyl))-2-[[(1 ,1 -dimethyl- ethyl)amino]carbonylJ-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]- carbonyl]-2-methylpropyl]carbamate in 1 ml of DMF was treated with 30 mg (0.18 mmol) of 3,4-dioxomethylenebenzyl chloride followed by 43 μl (0.24 mmol) of N,N-diisopropyl ethyl amine. The solution was stirred at room temperature over night. After stirring, the solution was partitioned between ethyl acetate and saturated sodium bicarbonate solution. The organic solution was washed with brine, dried over sodium sulfate, and concentrated in vacuo. The mixture was purified by chromatography on silica gel, using 5% methanoLmethylene chloride, to provide the title compound (yield: 31 mg; 26%).

MS: 737 (M+H)+.

1 H NMR (CDCI₃) δ 0.67 (d, J = 6.3 Hz, 3H), 0.84 (d, J = 6.3 Hz, 3H), 1.37

(s, 9H), 2.05 (hextet, J = 4.5 Hz, 1 H), 2.26 (m, 1 H), 2.41 (dd, J = 12.0, 3.0 Hz, 1 H), 2.55 (m, 1 H), 2.59 (dd, J = 9.3, 3.0 Hz, 2H), 2.72 (m, 2H), 2.73 (s, 3H), 2.76 (m, 1 H), 2.83 (m, 2H), 2.91 (m, 1 H), 3.35 (m, 1 H), 3.47 (s, 2H), 3.79 (m, 1 H), 3.86 (dd, J = 7.5, 6.0 Hz, 1 H), 4.22 (m, 1 H), 4.93 (m, 1 H), 5.62 (m, 2H), 5.65 (d, J = 3.0 Hz, 2H), 5.96 (s, 2H), 6.18 (d, J = 9.0 Hz, 1 H), 6.70 (d, J = 7.5 Hz, 1 H), 6.75 (s, 1 H), 6.78 (d, J = 7.5 Hz, 1 H), 7.12-7.22 (m, 6H), 8.19 (br s, 1 H).

Example 16 2-Methyl-4-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-[(2S⁾-4-f (3.4-dimethoxyl- phenyl)methyl]-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy- 1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 15B, substituting 3,4-dimethoxybenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 753 (M+H)+.

¹ H NMR (DMSO-de) δ 0.67 (d, J = 6.3 Hz, 3H), 0.69 (d, J = 6.3 Hz, 3H), 1.23 (s, 9H), 1.78 (hextet, J = 4.5 Hz, 1 H), 2.22 (m, 2H), 2.27 (m, 1 H), 2.32 (m, 1 H), 2.53 (m, 3H), 2.63 (s, 3H), 2.67 (m, 1 H), 2.87 (m, 2H), 2.98 (m, 1 H), 3.33 (m, 3H), 3.63 (m, 1 H), 3.72 (s, 3H), 3.73 (s, 3H), 4.05 (m, 1 H), 4.86 (d, J = 9.0 Hz, 1 H), 5.00 (s, 2H), 6.79 (d, J = 7.5 Hz, 1 H), 6.87 (s, 1 H), 6.89 (d, J = 7.5 Hz, 1 H), 7.10 (d, J = 6.0 Hz, 2H), 7.18 (d, J = 6.3 Hz, 2H), 7.26 (d, J = 7.5 Hz, 2H), 7.41 (s, 1 H), 7.53 (s, 1 H), 7.67 (d, J = 9.0 Hz, 1 H). Example 17 2-Msthyl-4-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R 3-[(2S 2-[[π .1 -dimethyl- ethvnamino]carbonvl]-4-r(4-fluorophenvnmethvl]-1 -piperazinvl]-2-hvdroxv-1 - (phenvlmethynpropvl]amino]carbonvl]-2-methvlpropvl]carbamate.

The title compound was prepared according to the method described in Example 15B, substituting 4-fluorobenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 711 (M+H)⁺. H NMR (CDCI₃) δ 0.68 (d, J = 6.3 Hz, 3H), 0.84 (d, J = 6.3 Hz, 3H), 1.35

(s, 9H), 2.06 (hextet, J = 6.0 Hz, 1 H), 2.28 (m, 1 H), 2.47 (m, 1 H), 2.55 (m, 1 H), 2.59 (m, 2H), 2.71 (m, 2H), 2.73 (s, 3H), 2.76 (m, 1 H), 2.83 (m, 2H), 2.90 (m, 1 H), 3.35 (m, 1 H), 3.43 (s, 2H), 3.80 (m, 1 H), 3.86 (dd, J = 7.5, 6.0 Hz, 1 H), 4.22 (septet, J = 4.5 Hz, 1 H), 5.10 (m, 1 H), 5.14 (s, 2H), 6.15 (d, J = 7.5 Hz, 1 H), 7.03 (t, J = 8.4 Hz, 2H), 7.12-7.22 (m, 8H), 8.21 (br s, 1 H).

Example 18 2-Ethyl-4-thiazolylmethyl-(1 S)-1 -FrFM S.2Rl-3-f.2S -r.3.4-dimethoxyl- Dhenvπmethyl]-2-[[(1 .1 -dimethylethv0amino]carbonyl]-1 -piperazinyl]-2-hvdroxy- 1 -(phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate.

18A. 2-Ethyl-4-thiazolylmethyl-(1 S)-1 -[[[(1 S.2R)-3-r(2SV4-[1 .1 -dimethylethyl- oxy(^,carbonvπ]-2-[[f1 .1 -dimethylethvhamino]carbonyl]-1 -piperazinyl]-2-hvdroxy-

1 -ι'phenvlmethvnpropvl]amino1carbonvl]-2-methvlpropvl]carbamate.

The title compound was prepared according to the method of Example 1 C, substituting [(2-ethyl-4-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2- isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine. This was followed by treatment of the product with trifluoroacetic acid according to the method of Example 1 D.

18B. 2-Ethyl-4-thiazolylmethyl-(1 SV1 -[[[(1 S.2RV3-ff2S 4-[f3.4-dimethoxyl- phenvπmethyl]-2-[[(1 .1 -dimethylethvπamino]carbonyl]-1 -piperazinyl]-2-hvdroxy- 1 -(^,phenylmethvnpropyl]aminolcarbonyl]-2-methylpropyl]carbamate.

The title compound was prepared by reacting 2-ethyl-4-thiazolylmethyl- (1 S)-1 -[[[(1 S,2R)-3-[(2S)-4-[1 ,1 -dimethylethyl-oxy(carbonyl)]-2-[[(1 ,1 -dimethyl- ethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]- carbonyl]-2-methylpropyl]carbamate, 98 mg (0.16 mmol), with 3,4- dimethoxybenzyl chloride, 30 mg (0.18 mmol), according to the method described in Example 15(B).

MS: 767 (M+H)⁺.

1 H NMR (CDCI₃) δ 0.67 (d, J = 6.3 Hz, 3H), 0.84 (d, J = 6.3 Hz, 3H), 1.36

(s, 9H), 1.40 (d, J = 7.5, 6H), 2.05 (hextet, J = 6.0 Hz, 1 H), 2.23 (m, 1 H), 2.48 (m, 1 H), 2.58 (dd, J = 12.0, 3.0 Hz, 2H), 2.71 (m, 2H), 2.83 (m, 1 H), 2.90 (m, 1 H), 3.05 (q, J = 7.5 Hz, 2H), 3.31 (m, 1 H), 3.35 (m, 1 H), 3.40 (AB, J = 15.0 Hz, 2H), 3.88 (s, 3H), 3.89 (s, 3H), 4.22 (m, 1 H), 4.88 (m, 1 H), 5.08 (m, 1 H), 5.16 (s, 2H), 6.11 (d, J = 7.5 Hz, 1 H), 6.77 (s, 1 H), 6.82 (s, 2H), 7.12-7.22 (m, 8H), 8.21 (br s, 1 H).

Example 19 2-(1 -Methylethvn-5-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-[.2S)-4-[(3.4-di- methoxylphenyl)methyl]-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2- hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

19A. 2-(1 -Methylethyl)-5-thiazolylmethyl-r(1 S)-1 -[[[(1 S.2R)-3-[(2S)-4-[1.1 - dimethylethyloxy(carbonyl)]-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazin- yl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbam- ate.

A solution of 1.5 g (3.3 mmol) of 1 -[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethyl- ethyloxy(carbonyl))-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hy- droxy-1 -(phenylmethyl)propyl]amine in 7 ml of THF and 7 ml of methylene chloride was prepared and treated with 1.0 g (3.3 mmol) of [(2-isopropyl-5-thia- zolyl)methyloxycarbonyl]-L-valine, 642 mg (3.3 mmol) of EDAC, 45 mg (0.33 mmol) of HOBT, and 466 μl (3.3 mmol) of triethyl amine. The solution was stirred at room temperature for 6 hours, and concentrated in vacuo. The residue was taken up with ethyl acetate, washed with equal portions (15 mL) of 3 N HCI solution, 10% aqueous NaHC03_, and saturated brine, dried over MgSθ4, and concentrated in vacuo. The resulting mixture was purified by chromatography on silica gel, using 5% methanoLmethylene chloride, to provide the title compound yield: 2.1 g, 88%).

MS: 731 (M+H)+.

1 H NMR (DMSO-d₆) δ 1.08 (d, J = 7 Hz, 6H), 2.78 (heptet, J = 7 Hz, 1 H), 9.06 (br s, 1 H), 9.30 (br s, 1 H).

19B. 2-(1 -Methylethyl)-5-thiazolylmethyl-[(1 S)-1 -[[[⁽ 1 S.2R)-3-[(2S)-4-[(3.4-di- methoxylphenyl)methyl]-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2- hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared in two steps. First, 2-(1 -methylethyl)-5- thiazolylmethyl-[(1 S)-1 -[[[(1 S,2R)-3-[(2S)-4-[1 ,1 -dimethylethyloxy(carbonyl)]-2- [[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)- propyl]amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1 D. This was followed by akylation of the product according to the method of Example 1 E, substituting 3,4-dimethylbenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 781 (M+H)+.

¹ H NMR (CDCI₃) δ 0.72 (d, J = 6.3 Hz, 3H), 0.86 (d, J = 6.3 Hz, 3H), 1.37 (s, 9H), 1.40 (d, J = 6.3, 6H), 2.01 (hextet, J = 6.0 Hz, 1 H), 2.22 (dt, J = 7.5, 3.0 Hz, 1 H), 2.43 (m, 1 H), 2.55 (m, 2H), 2.73 (m, 3H), 2.88 (m, 2H), 2.93 (m, 1 H), 3.30 (heptet, J = 6.3 Hz, 1 H), 3.38 (m, 1 H), 3.41 (AB, J = 15.0 Hz, 2H), 3.80 (m, 1 H), 3.87 (s, 3H), 3.88 (s, 3H), 4.22 (septet, J = 4.5 Hz, 1 H), 5.07 (d, J = 8.4 Hz, 1 H), 5.20 (d, J = 3.0 Hz, 2H), 6.06 (d, J = 9.0 Hz, 1 H), 6.76 (s, 1 H), 6.82 (s, 2H), 7.12-7.22 (m, 7H), 7.61 (s, 1 H), 8.28 (br s, 1 H).

Example 20 2-Ethyl-5-thiazolylmethyl-M S⁾-1 -[[[⁽1 S.2R⁾-3-[(2S -[(3.4-dimethoxyl- phenvπmethyl]-2-[[f1 .1 -dimethylethvπamino]carbonyl]-1 -piperazinyl]-2-hvdroxy- 1 -(phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate. 20A. 2-Ethyl-5-thiazolylmethvt-.1 SH -πTM S.2R⁾-3-f.2S 4-M .1 -dimethylethyl- oxy(carbonyl)]-2-[[(1.1 -dimethylethyl)amino]carbonvl1-1 -piperazinvl]-2-hvdroxv- 1 - henvlmethvhpropvl1amino]carbonvl]-2-methvlpropvl]carbamate.

The title compound was prepared according to the method described in Example 19A, substituting [(2-ethyl-5-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

20B. 2-Ethyl-5-thiazolylmethyl-π SH -[[[(1 S.2RV3-f(2S)-4-f f3.4-dimethoxyl- phenvhmethyl]-2-[[(1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hvdroxy- 1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared in two steps. First, 2-ethyl-5-thiazolyl- methyl-(1 S)-1 -[[[(1 S,2R)-3-[(2S)-4-[1 ,1 -dimethylethyloxy(carbonyl)]-2-[[(1 ,1 - dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)- propyl]amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1 D. This was followed by akylation of the product according to the method of Example 1 E, substituting 3,4-dimethylbenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 767 (M+H)+.

1 H NMR (CDCI₃) δ 0.73 (d, J = 6.3 Hz, 3H), 0.86 (d, J = 6.3 Hz, 3H), 1.37 (s, 9H), 1.38 (t, J = 7.5, 3H), 2.02 (m, 1 H), 2.23 (dt, J = 7.5, 3.0 Hz, 1 H), 2.45 (m, 1 H), 2.56 (m, 2H), 2.72 (m, 2H), 2.77 (m, 1 H), 2.89 (m, 2H), 3.02 (q, J = 7.5 Hz, 2H), 3.39 (t, J = 3.3 Hz, 1 H), 3.41 (AB, J = 15.0 Hz, 2H), 3.81 (m, 2H), 3.88 (s, 3H), 3.89 (s, 3H), 4.21 (m, 1 H), 5.06 (d, J = 9.0 Hz, 1 H), 5.20 (d, J = 5.4 Hz, 2H), 6.06 (d, J = 9.0 Hz, 1 H), 6.76 (s, 1 H), 6.82 (s, 2H), 7.13-7.23 (m, 7H), 7.61 (s, 1 H), 8.27 (br s, 1 H).

Example 21 2-Methyl-5-thiazolylmethyl-rπ SH -\\\U S.2R)-3-[(2S)-4-[(3.4-dimethoxyl- phenyl)m9thyl1-2-[[(1 .1 -dimethylethyl)amino|carbonyl1-1 -piperazinyl]-2-hvdroxy- 1 -ι^,phenylmethvπpropyl]aminolcarbonyl]-2-methylpropyl1carbamate. 21 A. 2-Mθthyl-5-thiazolylmθthyl-[π S)-1 -fffπ S.2R)-3-[(2S)-4-[1 .1 -dimθthylθthyl- oxyι^,carbonvni-2-[rf1.1 -dimethvlethvnamino]carbonyl]-1 -piperazinyl]-2-hvdroxy- 1 - henylmethvnpropyl]amino1carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 19A, substituting [(2-methyl-5-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

21 B. 2-Methyl-5-thiazolylmethyl-r (1 SH -[[[(1 S.2R)-3-rf2S)-4-[(3.4-dimethoxyl- phenyl)methyl]-2-[[(1 .1 -dimethylethvπamino]carbonyl]-1 -piperazinyl]-2-hvdroxy- 1 -φhenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared in two steps. First, 2-methyl-5- thiazolylmethyl-[(1 S)-1 -[[[(1 S,2R)-3-[(2S)-4-[1 ,1 -dimethylethyloxy(carbonyl)]-2- [[(1 ,1 -dimethylethyl)amiπo]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)- propyl]amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1 D. This was followed by akylation of the product according to the method of Example 1 E, substituting 3,4-dimethylbenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 753 (M+H)+.

¹ H NMR (CDCI3) δ 0.72 (d, J = 6.3 Hz, 3H), 0.86 (d, J = 6.3 Hz, 3H), 1.46 (s, 9H), 2.01 (hextet, J = 6.0 Hz, 1 H), 2.22 (dt, J = 7.5, 3.0 Hz, 1 H), 2.43 (dd, J = 12.0, 3.0 Hz, 1 H), 2.56 (m, 2H), 2.69 (s, 3H), 2.72 (m, 1 H), 2.77 (m, 1 H), 2.86 (m, 2H), 2.92 (m, 1 H), 3.39 (t, J = 3.0 Hz, 1 H), 3.41 (AB, J = 15.0 Hz, 2H), 3.80 (m, 2H), 3.87 (s, 3H), 3.88 (s, 3H), 4.21 (septet, J = 4.5 Hz, 1 H), 5.06 (d, J = 9.0 Hz, 1 H), 5.19 (AB, J = 9.6 Hz, 2H), 6.07 (d, J = 9.0 Hz, 1 H), 6.75 (s, 1 H), 6.82 (s, 2H), 7.12-7.22 (m, 7H), 7.58 (s, 1 H), 8.27 (br s, 1 H).

Example 22 N'-[(1 RH -\\\C\ S.2R)-3-[f2S)-2-[[M .1 -Dimethylethvhamino]carbonyl]-4-f5-thia- zolylmethviπ -piperazinyl]-2-hvdroxy-1 -ι'phenylmethvhpropyl1amino1carbonyl]- 2-methvlpropvl]-N-methvl-N-(5-thiazolvlmethvnurea.

22A. N'-[n Rn -r[[M S.2R)-3-[(2S)-2-[[(1 .1 -Dim9thyl9thyl)amino]carbonyl]-4-f1 .1 - dimethylsthyloxy(carbonvn]-1 -piperazinyl]-2-hvdroxv-1 -(phenylm9thyl)propyl]- amino]carbonyl]-2-methylpropyl]-N-methyl-N-('5-thiazolylmethvnurea.

The title compound was prepared according to the method described in Example 19A, substituting [N-methyl-(5-thiazolyl)methylaminocarbonyl]-D- valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

22B. N'-[(1 R)-1 -[[[π S.2R)-3-[(2S)-2-r[(1 .1 -Dimθthylθthyl)amino1carbonyl]-4-.5- thiazolylmethylH -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethvπurea.

The title compound was prepared in two steps. N'-[(1 R)-1 -[[[(1 S,2R)-3- [(2S)-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-4-[1 ,1 -dimethylethyloxy(carbonyl)]- 1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2- methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea was treated with trifluoroacetic acid according to the method described in Example 1 D. This was followed by akylation of the product according to the method of Example 1 E, substituting 5-thiazolyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 699 (M+H)+.

¹ H NMR (CDCI₃) δ 0.58 (d, J = 6.3 Hz, 3H), 0.66 (d, J = 6.3 Hz, 3H), 1.38 (s, 9H), 1.75 (m, 1 H), 2.35 (m, 1 H), 2.60 (m, 2H), 2.70 (m, 2H), 2.85 (m, 2H), 2.86 (s, 3H), 2.92 (m, 3H), 3.30 (t, J = 3.3 Hz, 1 H), 3.74 (s, 2H), 3.88 (m, 1 H), 3.97 (dd, J = 8.4, 6.0 Hz, 1 H), 4.28 (m, 2H), 4.53 (br s, 1 H), 4.65 (d, J = 3.3 Hz, 2H), 5.03 (d, J = 7.5, 1 H), 6.09 (d, J = 9.0 Hz, 1 H), 7.17-7.27 (m, 5H), 7.73 (s, 2H), 8.73 (s, 1 H), 8.80 (s, 1 H).

Example 23 N'-fM SH -[[[⁽ 1 S.2RK3-[⁽2S)-2-[[M .1 -Dimethylethyl)amino]carbonyl]-4-(5- thiazolylmethyl)-1 -piperazinyl]-2-hvdroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethvπurea. 23A. N'-[M SH -\\\(1 S.2RV3-[f2SV2-[[(1 .1 -Dimethylethvnamino]carbonyl]-4- [1 .1 -dimethylethyloxyfcarbonvh]-1 -piperazinyl]-2-hvdroxy-1 -(phenylmethyl)- propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-f5-thiazolylmethyl)urea.

The title compound was prepared according to the method described in Example 19A, substituting [N-methyl-(5-thiazolyl)methylaminocarbonyl]-L- valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

23B. N'-[(1 S)-1 -rff(1 S.2R⁾-3-[(2S)-2-[[(1 .1 -Dimethylethyl)amino]carbonyl]-4-(5- thiazolylmethyl)-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethy0urea.

The title compound was prepared in two steps. First, N'-[(1 S)-1 - [[[(1 S,2R)-3-[(2S)-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-4-[1 ,1 -dimethyl- ethyloxy(carboπyl)]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]car- bonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea was treated with trifluoroacetic acid according to the method described in Example 1 D. This was followed by akylation of the product according to the method of Example 1 E, substituting 5-thiazolyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

MS: 699 (M+H)+.

¹ H NMR (CDCI3) δ 0.78 (d, J = 6.3 Hz, 3H), 0.87 (d, J = 6.3 Hz, 3H), 1.49 (s, 9H), 2.02 (m, 1 H), 2.33 (m, 2H), 2.57 (m, 2H), 2.75 (m, 2H), 2.82 (s, 3H), 2.89 (m, 3H), 3.38 (t, J = 3.0 Hz, 1 H), 3.73 (d, J = 3.3 Hz, 2H), 3.79 (m, 1 H), 4.03 (dd, J = 8.4, 6.3 Hz, 1 H), 4.20 (m, 1 H), 4.66 (AB, J = 15 Hz, 2H), 4.78 (m, 1 H), 4.81 (d, J = 8.4 Hz, 2H), 6.11 (d, J = 9.0 Hz, 1 H), 7.11 -7.23 (m, 5H), 7.73 (s, 1 H), 7.76 (s, 1 H), 7.82 (br s, 1 H), 8.75 (s, 1 H), 8.80 (s, 1 H).

Example 24 5-Thiazolylmethyl-[(1 SH -[[[⁽1 S.2R⁾-3-[(2S)-2-[[.1.1 -dimethylethyl)amino]car- bonyl]-4-(^,phenylmethviπ -piperazinyl]-2-hvdroxy-1 - henylmethvπpropyl]- amino]carbonyl]-2-mθthylpropyl]carbamatθ.

24A. 5-Thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-f(2SΪ-2-[f(1 .1 -dimethylethvnamino]- carbonyl]-4-[1 .1 -dimethylethyloxy.carbonyl)1-1 -piperazinyl]-2-hvdroxy-1 - (^,phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamate. The title compound was prepared according to the method described in Example 19A, substituting [(5-thiazolyl)methyloxycarbonyl]-L-valine, in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

24B. 5-Thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-[(2S)-2-[[(1 .1 -dimethylethvnamino]- carbonyl]-4-(phenylmethyl)-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyπpropyl]- amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared in two steps. First, 5-thiazolylmethyl- [(1 S)-1 -[[[(1 S,2R)-3-[(2S)-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-4-[1 ,1 - dimethylethyloxy(carbonyl)]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]- amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1 D. This was followed by akylation of the product according to the method of Example 1 E, substituting benzyl bromide in place of 3,4-dioxomethylenebenzyl chloride.

MS: 699 (M+H)+.

¹ H NMR (CDCI₃) δ 0.78 (d, J = 6.3 Hz, 3H), 0.87 (d, J = 6.3 Hz, 3H), 1.49 (s, 9H), 2.02 (m, 1 H), 2.33 (m, 2H), 2.57 (m, 2H), 2.75 (m, 2H), 2.82 (s, 3H), 2.89 (m, 3H), 3.38 (t, J = 3.0 Hz, 1 H), 3.73 (d, J = 3.3 Hz, 2H), 3.79 (m, 1 H), 4.03 (dd, J = 8.4, 6.3 Hz, 1 H), 4.20 (m, 1 H), 4.66 (AB, J = 15 Hz, 2H), 4.78 (m, 1 H), 4.81 (d, J = 8.4 Hz, 2H), 6.11 (d, J = 9.0 Hz, 1 H), 7.1 1 -7.23 (m, 5H), 7.73 (s, 1 H), 7.76 (s, 1 H), 7.82 (br s, 1 H), 8.75 (s, 1 H), 8.80 (s, 1 H).

Example 25 5-Thiazolylmethyl-(1 SH -[ffM S.2R)-3-fr2S)-2-f[(1 .1 -dimethylethyl)amino]car- bonyl]-4-(5-thiazolylmethyl)-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]- amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 24B, by akylation of 5-thiazolylmethyl-[(1 S)-1 -[[[(1 S,2R)-3-[(2S)-2- [[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)- propyl]amino]carbonyl]-2-methylpropyl]carbamate with 5-thiazolyl methyl chloride in place of benzyl bromide.

MS: 686 (M+H)+.

¹ H NMR (CDCI3) δ 0.62 (d, J = 6.3 Hz, 3H), 0.86 (d, J = 6.3 Hz, 3H), 1.39 (s, 9H), 2.01 (m, 1 H), 2.30 (m, 1 H), 2.52 (m, 1 H), 2.67 (m, 2H), 2.71 (m, 1 H), 2.75 (m, 2H), 2.87 (m, 2H), 2.92 (m, 1 H), 3.29 (t, J = 3.0 Hz, 1 H), 3.74 (d, J = 4.5 Hz, 2H), 4.80 (m, 2H), 4.85 (m, 1 H), 4.21 (m, 1 H), 5.08 (t, J = 9.0 Hz, 1 H), 5.18 (d, J = 4.5 Hz, 1 H), 6.07 (d, J = 9.6 Hz, 2H), 7.1 1 -7.24 (m, 5H), 7.74 (s, 1 H), 7.88 (s, 1 H), 8.80 (s, 1 H), 8.81 (s, 1 H).

Example 26 N'-[π sπ-rrr(1 S.2RV3-frfn .1-Dimethylethvhamino]carbonyl](2-methylethvnpro- pvlamino1-2-hvdroxv-1 -(phenvlmethvnpropvl]amino]carbonyl]-2-methylpropyl]- N-mθthyl-N-[2-f1-mθthylθthvπ-4-thiazolylmθthyl]urθa.

Thθ titlθ compound was prepared according to the method described in Example 12A, by coupling N-methyl-N-[(((2-isopropyl-4-thia- zolyl)methyl)amino)-carbonyl]-L-valine with N-(3S-amino-2R-hydroxy-4-phenyl- butyl)-N-(2-methylpropyl)-N'-(1 ,1 -dimethylethyl)urea, prepared according to the method described in J. Med. Chem., 1993, 36, 288-291.

Elemental analysis:

Theory: C: 62.34, H: 8.44, N: 13.64

Found: C: 59.60, H: 7.37, N: 12.86

Example 27 2-M -Methylethvh-4-thiazolylmethyl-[f 1 SH -[[[(1 S.2R )-3-[[[.1 .1 -dimethylethvh- amino]carbonyl1(^,2-methylethvπpropylamino]-2-hvdroxy-1 -ι^/phenylmethvh- propyl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to thθ mθthod dθscribθd in Examplθ 26, by coupling N-[(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L- valine with N-(3S-amino-2R-hydroxy-4-phenylbutyl)-N-(2-methylpropyl)-N'-(1 ,1 - dimethyiethyl)urea, prepared according to the method described in J. Med. Chem., 1993, 36, 288-291.

Elemental analysis:

Theory: C: 61.69, H: 8.13, N: 11.61

Found: C: 60.80, H: 8.21 , N: 11.18 Example 28 2-(1 -Methylethyn-4-thiazolylmsthyl-M SH -[ffM S.2R)-3-[[(4-aminophenyl)sulfon- yl]M -methylethvπamino]-2-hvdroxy-1 -fphθnylmθthvπpropyl]amino]carbonyl]-2- mθthvlpropvl]carbamate.

The title compound was prepared in two steps. First, N-isobutyl-2(R)-hy- droxy-3(S)-amino-4-phenyl-(4'-nitrophenyl)sulfonamide, prepared according to the method described in J. Am. Chem. Soc, 1995, 117, 1181 -1182, was coupled with [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine according to the method described in Example 1 C. This was followed by hydrogenation of the product over Pd/C under 1 atmosphere of hydrogen . The catalyst was removed via filtration over a celite pad and the solvent was evaporated at reduced pressure to provide the title compound as a white foam.

MS: 674 (M+H)⁺.

¹ H NMR (DMSO-de) δ 0.65 (d, J = 6.3 Hz, 3H), 0.67 (d, J = 6.3 Hz, 3H), 0.77 (d, J = 6.0 HZ, 3H), 0.81 (t, J = 6.0 HZ, 3H), 1.32 (d, J = 6.3 Hz, 6H), 1.77 (m, 1 H), 1.90 (m, 1 H), 2.60 (m, 2H), 2.65 (m, 1 H), 2.72 (m, 1 H), 2.83 (d, J = 7.5 Hz, 1 H), 2.89 (d, J = 9.0 Hz, 1 H), 3.00 (m, 2H), 3.25 (m, 1 H), 3.61 (m, 1 H), 3.75 (dd, J = 9.0, 6.3 Hz, 1 H), 3.94 (m, 1 H), 4.96 (d, J = 6.3 Hz, 1 H), 5.02 (s, 2H), 5.98 (s, 2H), 6.60 (t, J = 9.0 Hz, 2H), 7.10 (m, 2H), 7.15-7.22 (m, 3H), 7.39 (d, J = 8.4 Hz, 2H), 7.43 (s, 1 H), 7.72 (d, J = 9.0 Hz, 1 H).

Example 29 N'-[(1 SH -[[[(1 S.2R)-3-[[(4-Aminophenyl)sulfonyl](1 -methylethvnamino]-2-hy- droxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2- (1 -methylethyl)-4-thiazolylmethyl]urea.

The title compound was prepared in two steps. First, N-isopropyl-2(R)- hydroxy-3(S)-amino-4-phenyl-(4'-nitrophenyl)sulfonamide was coupled with N- methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine according to the method described in Example 12A. This was followed by hydrogenation of the product over Pd/C under 1 atmosphere of hydrogen. The catalyst was removed via filtration over a celite pad and the solvent was evaporated at reduced pressure to provide the title compound as a white foam.

MS: 674 (M+H)+.

Elemental analysis: Theory: C: 58.93, H: 7.14, N: 12.50 Found: C: 58.30, H: 7.28, N: 12.1 1

Example 30 2-(1 -Methylethyl)-5-thiazolylmethyl-[(1 S)-1 -[[[(1 S.2R)-3-[(3S)-3-[[(1.1 -dimethyl- ethvπamino]carbonyl](4aα.8aα octahvdro-2-isoquinolinyl]-2-hvdroxy-1 - henvlmethvnpropvl]amino]carbonyl]-2-methylpropyl]carbamate.

The title compound was prepared according to the method described in Example 1 C, by coupling 2-(3(S)-amino-2(r?)-hydroxy-4-phenylbutyl)-/V-ret - butyldecahydro-(4aS,8aS)-isoquinoline-3(S)-carboxamide with [(2-isopropyl-4- thiazolyl)methyloxycarbonyl]-L-valine, prepared according to the method described in J. Org Chem., 1994, 59, 3656-3664.

Elemental analysis:

Theory: C: 65.01 , H: 8.35, N: 10.25

Found: C: 63.70, H: 8.19, N: 10.05

Example 31 N'-[⁽1 Sπ -r[[⁽1 S.2R⁾-3-[f3SV3-rr(1 .1 -Dimethylethvnamino]carbonyl](4aα.8a )- octahydro-2-isoquinolinyl]-2-hvdroxy-1 -(phenylmethyl)propyl]amino]carbonyl]- 2-mθthylpropyl]-N-methyl-N-[2-f1 -methylethylV5-thiazolylmethyl]urea.

The title compound was synthesized according to the method described in Example 12A by coupling of 2-(3(S)-amino-2(/?)-hydroxy-4-phenylbutyl)-N- ret -butyldecahydro-(4aS,8aS)-isoquinoline-3(S)-carboxamide with N-methyl- N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine which was prepared according to the method described in J. Org Chem., 1994, 59, 3656- 3664.

Elemental analysis:

Theory: C: 65.52, H: 8.62, N: 12.07

Found: C: 64.50, H: 8.72, N: 12.09

Example 32 N'-[.1 SH 417(1 S.2 -3-K2S.4m-2-ffπ .1 -Dimethylethvhamino]carbonyl]-4-f5- thiazolylmethoxyπ -piperidinyl]-2-hvdroxy-1 -fphenylmethvhpropyl]amino]- carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1 -methylethyl)-4-thiazolyimethyl]urea.

32A. 1 -cis-N-π .1 -DimethylethylH -M .1 -dimethyl8thyloxycarbonyl 4-(5-thia- zolylmθthyloxy^pipθridine-2-carboxamidθ.

1 -cis-N-(1 ,1 -Dimethylethyl)-1 -(1 ,1 -dimethylethyloxycarbonyl)-4-hydroxy- pipehdine-2-carboxamide was prepared according to the methods described in EP 560 268 A1. The hydroxy-carboxamide compound was treated with sodium hydride and 5-thiazolyl methyl chloride in DMF at 0 °C to provide the title compound.

32B. 1 -cis N-f 1.1 -Dimethylethvh-4-⁽5-thioazoylmethyloxy)piperidine-2-carbox- amide.

A solution of 1.24 g of 1 -cis-N-(1 ,1 -dimethylethyl)-1 -(1 ,1 -dimethylethyl- oxycarbonyl)-4-(5-thiazolylmethyloxy)piperidine-2-carboxamide in 10 ml of THF was prepared. The mixture was treated with 3 N HCI, and stirred at room temperature for 1 hour. After stirring, the reaction was it was partitioned between methylene chloride and saturated sodium bicarbonate. The organic layer was washed with brine, dried with Na2S04 and evaporated to dryness to provide 836 mg of desired product.

32C. N'-f.1 SV1 -[[[⁽1 S.2RV-3-r⁽2S.4R 2-rff1 .1 -Dimethylethyl)amino)carbonyl]-4- (5-thiazolylmethoxy)-1 -piperidinyl]-2-hvdroxy-1 -(phenylmethvπpropyl]amine.

The title compound was synthesized in two steps. N'-[(1 S)-1 -[[[(1 S,2R)- 3-[(2S,4R)-2-[[(1 ,1 -Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1 - piperidinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amine was prepared according to the method described in Example 1 A by using t-butyloxycarbonyl phenylalanylepoxide replacing N-benzyloxycarbonyl phenylalanylepoxide followed by column purification. The resulting product was treated with trifluoroacetic acid (TFA) in methylene chloride at room temperature. 32D. N'-[(1 SV1 -[[[M S.2R -3-[(2S.4RV2-[[(1 .1 -Dimethylethvhamino]carbonyl]-4-

(5-thiazolylmethoxyπ -piperidinyl]-2-hvdroxy-1 -(phenylmethvnpropyl]amino]- carbonyl]-2-mθthylpropyl]-N-methyl-N-[2-('1 -methylethvh-4-thiazolylmθthyl]urθa.

The title compound was synthesized by the method described in Example 12A using N'-[(1 S)-1 -[[[(1 S,2R)-3-[(2S,4R)-2-[[(1 ,1 -dimethyl- ethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1 -piperidinyl]-2-hydroxy-1 - (phenylmethyl)propyl]amine and N-methyl-N-[(((2-isopropyl-4- thiazolyl)methyl)amino)carbonyl]-L-valine.

MS: 756 (M+H)+.

1 H NMR (CDCI3) d 0.65 (d, J = 6.3 Hz, 3H), 0.92 (d, J = 6.3 Hz, 3H), 1.32 (d, J = 3.0 HZ, 3H), 1.34 (d, J = 3.0 Hz, 2H), 1.35 (s, 9H), 1.53 (m, 1 H), 1.58 (m, 1 H), 1.91 (m, 1 H), 2.20 - 2.37 (m, 4H), 2.67 (m, 1 H), 2.69 (m, 1 H), 2.80 (dd, J = 11.4, 3.0 Hz, 1 H), 2.87 (dd, J = 15.0, 3.3 Hz, 1 H), 2.97 (s, 3H), 3.21 (heptet, J = 6.3 Hz, 1 H), 3.40 (t, J = 4.5 Hz, 1 H), 3.44 (m, 1 H), 3.91 (t, J = 5.7 Hz, 1 H), 4.03 (m, 2H), 4.12 (m, 1 H), 4.35 (AB, J = 15.0 Hz, 2H), 4.78 (AB, J = 12.0 Hz, 2H), 6.48 (d, J = 8.1 Hz, 1 H), 6.61 (s, 1 H), 7.00 (s, 1 H), 7.09-7.18 (m, 6H), 7.78 (s, 1 H), 8.79 (s, 1 H).

Example 33 2-M -Methylethvn-4-thiazolylmethyl [M SH -fRM S.2R 3-f f2S.4RV2-[[f1 .1 -di- methylethvhamino]carbonyl]-4-(5-thiazolylmethoxyH -piperidinyl]-2-hvdroxy-1 - (phenvlmethvnpropvl]amino]carbonvl]-2-methvlpropvl]carbamate.

The title compound is prepared according to the procedure described in example 32C using N-[(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine in place of N-methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine as a coupling reagent.

Example 4 S 2-M -Methvlethvn-4-thiazolvlmethvl] fM SH -\\\h S.2R)-3-[(2S)-4-(1 .3-benzo- dioxol-5-vimethvh-2-[[f 1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hv- droxy-1 -⁽phenylmethvπpropyl]amino]carbonyl]-2-methylpropyl]carbamothioate

34A. 2-lsopropyl-4-(methanesulfonyloxymethvπthiazole. A solution of 1.2 mmol of 4-(hydroxymethyl)-2-isopropylthiazole and 1.3 mmol of diisopropylethylamine in 20 ml of dichloromethane is cooled to -20°C and treated dropwise with 1.3 mmol of methanesulfonyl chloride. The resulting mixture is stirred for 1 hour, quenched with aqueous citric acid, separated, dried over Na2Sθ4, and concentrated in vacuo to provide the title compound.

34B. 2-lsopropyl-4-(mercaptomsthyl)thiazol6.

A mixture of 0.8 mmol of the product prepared in Example 34A and 1.0 mmol of sodium hydrosulfide hydrate in 20 ml of THF is heated at reflux until analysis by thin layer chromatography indicates consumption of the starting material. The resulting mixture is allowed to cool, concentrated in vacuo, partitioned between dichloromethane and water, dried over Na2S04, and concentrated to provide the crude compound.

34C. N-((2-lsopropyl-4-thiazolvl)thiomethoxycarbonyl)valinB methvl ester.

A solution of 2.18 g (15 mmol) of 2-lsopropyl-4-(mercaptomethyl)thiazole, 15.8 mmol of α-isocyanato-valine methyl ester and 1.5 mmol of 4-dimethyl- aminopy dine in 75 ml of dichloromethane is heated at reflux for about 5 hours. The resulting solution is washed successively with 10% citric acid, aqueous NaHCθ3 and brine, dried over Na2S04, and concentrated in vacuo. Silica gel chromatography of the residue using 5% ethyl acetate in chloroform will provide the title compound.

34D. N-f(2-lsopropyl-4-thiazolvπthiomethoxycarbonyl aline.

A solution of product, prepared in Example 34C in dioxane is treated with 0.50 M aqueous LiOH. The resulting solution is stirred at ambient temperature for about 30 minutes, treated with 1 M HCI, and concentrated in vacuo. The residue is taken up in dichloromethane, washed with water, dried over Na2S04, and concentrated in vacuo to provide the title compound.

34E. S-f2-(1 -Methylethvn-4-thiazolylmethyl [⁽1 SH -[[[(1 S.2R)-3-[(2S)-2-[[(1.1 - dimethylethvπamino]carbonyl-4-[[(1 .1 -dimethylethvπcarbonyl]oxy]-1 - piperazinyl]-2-hvdroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2- methylpropyl]carbamothioate. The title compound is prepared according to the method described in Example 1 C, substituting N-((2-lsopropyl-4- thiazolyl)thiomethoxycarbonyl)valine in place of [(2-isopropyl-4-thia- zolyl)methyloxycarbonyl]-L-valine.

34F. S-[2-f 1 -Msthvlethvn-4-thiazolvlmethvirM SH -[[[(1 S.2R)-3-\(2S)-2-\\h .1 - dimsthylethyl)amino]carbonvl]-1 -piperazinvl1-2-hvdroxv-1 -fph9nylmethyl)- propyl]amino]carbonyl]-2-methylpropyl]carbamothioate.

The title compound is prepared according to the method described in Example 1 D, substituting S-[2-(1 -methylethyl)-4-thiazolylmethyl] [(1 S)-1 - [[[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethylethyloxy(carbonyl))-2-[[(1 ,1 - dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)- propyl]amino]carbonyl]-2-methylpropyl]carbamothioate in place of 2-(1 -methyl- ethyl)-4-thiazolylmethyl-(1 S)-1 -[[[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethyl- ethyloxy(carbonyl))-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hy- droxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyI]carbamate.

34G. S-[2-(1 -Methylethvn-4-thiazolylmethyl] [(1 S)-1 -[[[(1 S.2R)-3-[ 2S -.1.3- benzodioxol-5-ylmethvπ-2-[[ι^,1 .1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]- 2-hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl1carbamo- thioate

The title compound is prepared according to the method described in Example 1 E, substituting S-[2-(1 -methylethyl)-4-thiazolylmethyl] [(1 S)-1 - [[[(1 S,2R)-3-[(2S)-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2- hydroxy-1 -(phenyimethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamo- thioate in place of 2-(1 -methylethyl)-4-thiazolylmethyl-(1 S)-1 -[[[(1 S,2R)-3-[(2S)- 2-[[(1 ,1 -dimethylethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenyl- methyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

Example 35

4-π .3-Benzodioxol-5-ylmethvn-N-π .1 -dimethylethylV1 -r(2R.3SV3-[ff2S)-2-rr3- [2-(1 -methvlethvn-4-thiazolvl]-1 -oxopropvl]amino]-3-methvl-1 -oxobutvl]amino]- 2-hvdroxy-4-phenvlbutvl]-2-piperazinecarboxamide.

35A. 2-lsopropylthiazole-4-carboxaldehvde.

A solution of 3.1 g (15.6 mmol) of ethyl 2-isopropylthiazole-4-carboxylate in 50 ml of dichloromethane was cooled under N2 atmosphere to -78°C and treated dropwise with 15.6 ml (23.4 mmol) of a 1.5 M solution of diisobutylaluminum hydride in toluene over a period of 1.5 h. After being stirred for an additional 0.5 h, the solution was quenched with 5 ml of methanol followed by 15 ml of aqueous Rochelle's salt. The resulting mixture was partitioned between chloroform and aqueous Rochelle's salt, dried over Na2Sθ4, and concentrated to provide 1.37 g (56%) of the crude desired compound, Rf 0.47 (20% ethyl acetate in hexane).

1 H NMR (CDCI3) d 1.45 (d, J = 7 Hz, 6 H), 3.39 (heptet, J = 7 Hz, 1 H),

8.07 (s, 1 H), 10.00 (s, 1 H).

Mass spectrum: (M + H)⁺ = 156.

35B. (E Ethvl 3-f2-lsopropvl-4-thiazolvnpropenoate.

A slurry of 60% NaH (18 mmol) in mineral oil was washed with hexane, decanted under N2 atmosphere, and diluted with 25 ml of THF. The resulting mixture was cooled to 0°C, treated portionwise with 3.24 ml (16.4 mmol) of triethylphosphonoacetate. After addition, the solution was stirred for 10 minutes, treated with 1.37 g (8.84 mmol) of 2-isopropylthiazole-4- carboxaldehyde in 25 ml of THF, allowed to warm to ambient temperature for 25 minutes, and quenched with 100 ml of saturated aqueous NH4CI. The mixture was extracted with three 100 ml portions of ethyl acetate, dried over Na2Sθ4, and concentrated in vacuo. Silica gel chromatography of the residue using 5- 10% ethyl acetate in hexane provided 1.61 g (81 %) of the desired compound, Rf 0.64 (20% ethyl acetate in hexane). ¹ H NMR (CDCI3) d 1.33 (t, J = 7 Hz, 3 H), 1.42 (d, J = 7 Hz, 6 H), 3.32 (heptet, J = 7 Hz, 1 H), 4.26 (q, J = 7 Hz, 2 H), 6.75 (d, J = 15 Hz, 1 H), 7.29 (s, 1 H), 7.57 (d, J = 15 Hz, 1 H).

35C. Methyl 3-(2-lsopropyl-4-thiazolyl)propanoate.

A solution of 225 mg (1 mmol) of (E)-ethyl 3-(2-isopropyl-4-thiazolyl)- propenoate in 10 ml of freshly distilled (from calcium hydride) methanol and 1 ml of dry THF is treated with 49 mg (2 mmol) of magnesium turnings. The mixture is stirred for 20 minutes, and the magnesium is consumed. The resulting solution is poured over cold aqueous HCI, basified to pH 8 with NaHCθ3, extracted with ethyl acetate, dried over Na2Sθ4, and concentrated. Silica gel chromatography using 10% ethyl acetate in hexane provides a mixture of the desired compound and methyl 3-(2-isopropyl-4- thiazolinyl)propanoate.

35D. 3-(^,2-lsoDropyl-4-thiazolyl)propanoic Acid.

A solution of product, prepared in Example 35C in dioxane is treated with 0.50 M aqueous LiOH. The resulting solution is stirred at ambient temperature for about 30 minutes, treated with 8.7 ml of 1 M HCI, and concentrated in vacuo. The residue is taken up in dichloromethane, washed with water, dried over Na2Sθ4, and concentrated in vacuo to provide the title compound.

35E. N-(1 .1 -DimBthylethvh-4-[fn .1-dimethyl6thyncarborιyl]oxy]-1-ff2R.3S)-3- [[(2S)-2-[[3-[2-f 1 -methylethyl)-4-thiazolyl1-1 -oxopropyl]amino]-3-methyl-1 -oxo- butvl]amino]-2-hvdroxv-4-phenvlbutvl]-2-piperazinecarboxamide.

A solution of 3-(2-isopropyl-4-thiazolyl)propanoic acid, 1.1 equivalents of α-isocyanato-valine methyl ester and 4-dimethylaminopyridine (catalytic) in of dichloromethane is heated at reflux for about 5 hours. The resulting solution is washed successively with 10% citric acid, aqueous NaHCθ3 and brine, dried over Na2S04, and concentrated in vacuo. Silica gel chromatography of the residue using 5% ethyl acetate in chloroform to provide the title compound. 35F. N-(1 .1-Dimsthylethyl)-1 -f.2R.3S⁾-3-[[f2S⁾-2-[[3-f2-n -methylethyn-4-thia- zolvl1-1 -oxopropvl]amino1-3-methvl-1 -oxobutvl]amino1-2-hvdroxv-4- phenylbutvl]-2-piperazinecarboxamide.

The title compound is prepared according to the method described in Example 1 C, substituting N-((2-lsopropyl-4-thiazolyl)propionyl)valine in place of [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine.

35G. N-(1.1-DimethylethylV1 - (2R.3SV3-f[(2SV2-rr3-r2-n-methylethvn-4- thiazolvl]-1 -oxopropvl]amino]-3-methvl-1 -oxobutvl]amino]-2-hvdroxv-4- phenvlbutvl]-2-piperazinecarboxamide.

The title compound is prepared according to the method described in Example 1 D, substituting 4-(1 ,1 -dimethylethyloxy(carbonyl))-N-(1 ,1 - dimethylethyl)-1 -[(2R,3S)-3-[[(2S)-2-[[3-[2-(1 -methylethyl)-4-thiazolyl]-1 - oxopropyl]amino]-3-methyl-1 -oxobutyl]amino]-2-hydroxy-4-phenylbutyl]-2- piperazinecarboxamide in place of 2-(1 -methylethyl)-4-thiazolylmethyl-(1 S)-1 - [[[(1 S,2R)-3-[(2S)-4-(1 ,1 -dimethylethyloxy(carbonyl))-2-[[(1 ,1 -dimethyl- ethyl)amino]carbonyl]-1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]- carbonyl]-2-methylpropyl]carbamate .

35H. 4-(1.3-Benzodioxol-5-ylmethvn-N-M .1 -dimethylethylH -[(2R.3S)-3-[[(2SV 2-[[3-[2-(1 -methylethvB-4-thiazolyl]-1 -oxopropyl]amino]-3-methyl-1 -oxobutyl]- amino]-2-hvdroxy-4-phenylbutyl]-2-piperazinecarboxamide.

The title compound is prepared according to the method described in Example 1 E, substituting N-(1 ,1 -dimethylethyl)-1 -[(2R,3S)-3-[[(2S)-2-[[3-[2-(1 - methylethyl)-4-thiazolyl]-1 -oxopropyl]amino]-3-methyl-1 -oxobutyl]amino]-2-hy- droxy-4-phenylbutyl]-2-piperazinecarboxamide in place of 2-(1 -methylethyl)-4- thiazolylmethyl-(1 S)-1 -[[[(1 S,2R)-3-[(2S)-2-[[(1 ,1 -dimethylethyl)amino]carbonyl]- 1 -piperazinyl]-2-hydroxy-1 -(phenylmethyl)propyl]amino]carbonyl]-2-methyl- propyl]carbamate.

Fluorogenic Assay for Screening Inhibitors of HIV Protease The inhibitory potency of the compounds of the invention can be determined by the following method:

A compound of the invention is dissolved in DMSO. A small aliquot is diluted with DMSO to 100 times the final concentration desired for testing. The test is carried out in a 6 X 50 mm tube in a total volume of 300 microliters. The final concentrations of the components in the reaction buffer are: 125 mM sodium acetate, 1 M sodium chloride, 5 mM dithiothreitol, 0.5 mg/ml bovine serum albumin, 1.3 μM fluorogenic substrate, 2% (v/v) dimethylsulfoxide, pH 4.5. After addition of inhibitor, the reaction mixture is placed in the fluorometer cell holder and incubated at 30°C for several minutes. The reaction is initiated by the addition of a small aliquot of cold HIV protease. The fluorescence intensity (excitation 340 nM, emmision 490 nM) is recorded as a function of time. The reaction rate is determined for the first six to eight minutes. The observed rate is directly proportional to the moles of substrate cleaved per unit time. The percent inhibition is 100 X (1 - (rate in presence of inhibitor)/(rate in absence of inhibitor)).

Fluorogenic substrate: Dabcyl-Ser-Gln-Asn-Tyr-Pro-Ile-Nal- Gln-EDAΝS wherein DABCYL = 4-(4-dimethylamino- phenyl)azobenzoic acid and EDAΝS = 5~((2-aminoethyl)amino)- naphthalene-1 -sulfonic acid. All compounds tested at 1.0 nM were found to have an IC50 of 100%. The test compounds were then tested at a concentration of 0.5 nM. The results are reported as percent inhibition, in Table 1 , below.

TABLE 1

Example No. % Inhib. (0.5 nM)

Example 1 60%

Example 2 66%

Example 3 64%

Example 4 67%

Example 5 69%

Example 6 60%

Example 7 73%

Example 8 54%

Example 9 65%

Example 10 70%

Example 11 62%

Example 12 61%

Example 13 66%

Example 14 63%

Example 15 45%

Example 16 49%

Example 17 50%

Example 18 82%

Example 19 51%

Example 20 37%

Example 21 44%

Example 23 51%

Example 24 40%

Example 25 45%

Example 26 42%

Example 27 50%

Example 28 77%

Example 29 64%

Example 30 55%

Example 31 48%

Example 32 66%

Table 1 shows the inhibitory potencies of compounds of the invention against HIV-1 protease.

Antiviral Activity

The anti-HIV activity of the compounds of the invention can be determined in MT4 cells according to the procedure of Kempf, et. al. (Antimicrob. Agents Chemother. 1991 , 35, 2209). The IC50 is the concentration of compound that gives 50% inhibition of the cytopathic effect of HIV. The LC50 is the concentration of compound at which 50% of the cells remain viable.

TABLE 2

The compounds of the present invention can be used in the form of salts derived from inorganic or organic acids. These salts include but are not limited to the following: acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate, digluconate, cyclopentanepropionate, dodecylsulfate, ethanesulfonate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxy- ethanesulfonate (isethionate), lactate, maleate, methanesulfonate, nicotinate, 2- naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate, 3- phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, p-toluenesulfonate and undecanoate. Also, the basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides, such as methyl, ethyl, propyl, and butyl chloride, bromides, and iodides; dialkyl sulfates like dimethyl, diethyl, dibutyl, and diamyl sulfates, long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides, aralkyl halides like benzyl and phenethyl bromides, and others. Water or oil-soluble or dispersible products are thereby obtained.

Examples of acids which may be employed to form pharmaceutically acceptable acid addition salts include such inorganic acids as hydrochloric acid, sulfuric acid and phosphoric acid and such organic acids as oxalic acid, maleic acid, succinic acid and citric acid. Other salts include salts with alkali metals or alkaline earth metals, such as sodium, potassium, calcium or magnesium or with organic bases.

Preferred salts of the compounds of the invention include hydrochloride, methanesulfonate, sulfonate, phosphonate and isethionate.

The compounds of the present invention can also be used in the form of esters. Examples of such esters include a hydroxyl-substituted compound of formula I or II which has been acylated with a blocked or unblocked amino acid residue, a phosphate function, a hemisuccinate residue, an acyl residue of the formula R¹⁴C(0)- or R¹⁴C(S)- wherein R¹⁴ is hydrogen, lower alkyl, haloalkyl, alkoxy, thioalkoxy, alkoxyalkyl, thioalkoxyalkyl or haloalkoxy, or an acyl residue of the formula R^a-C(R^b)(R^d)-C(0)- or Ra-C(R^b)(R^d)-C(S)- wherein Rb and Rd are independently selected from hydrogen or lower alkyl and R^a is -N(R^e)(R^f), OR^e or -SR^e wherein R^e and R^f are independently selected from hydrogen, lower alkyl and haloalkyl, or an amino-acyl residue having the formula R15NH(CH₂)2NHCH₂C(0)- or R15NH(CH₂)₂0CH₂C(0)- wherein R15 is hydrogen, lower alkyl, arylalkyl, cycloalkylalkyl, alkanoyl, benzoyl or an α- amino acyl group. The amino acid esters of particular interest are glycine and lysine; however, other amino acid residues can also be used, including those wherein the amino acyl group is -C(0)CH₂NR¹⁶R¹⁷ wherein R¹⁶ and R¹⁷ are independently selected from hydrogen and lower alkyl, or the group -NR¹⁶R¹⁷, where R¹⁶R¹⁷, taken together, forms a nitrogen containing heterocyclic ring. These esters serve as pro-drugs of the compounds of the present invention and serve to increase the solubility of these substances in the gastrointestinal tract. These esters also serve to increase solubility for intravenous administration of the compounds. Other prodrugs include a hydroxyl-substituted compound of formula I or II wherein the hydroxyl group is functionalized with a substituent of the formula -CH(R¹8)OC(0)R¹9 or -CH(R¹⁸)OC(S)R¹⁹ wherein R19 is lower alkyl, haloalkyl, alkoxy, thioalkoxy or haloalkoxy and R¹⁸ is hydrogen, lower alkyl, haloalkyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl or dialkylaminocarbonyl. Such prodrugs can be prepared according to the procedure of Schreiber (Tetrahedron Lett. 1983, 24, 2363) by ozonolysis of the corresponding methallyl ether in methanol followed by treatment with acetic anhydride.

The prodrugs of this invention are metabolized in vivo to provide the hydroxyl-substituted compound of formula I or II . The preparation of the prodrug esters is carried out by reacting a hydroxyl-substituted compound of formula I or II with an activated amino acyl, phosphoryl, hemisuccinyl or acyl derivative as defined above. The resulting product is then deprotected to provide the desired pro-drug ester. Prodrugs of the invention can also be prepared by alkylation of the hydroxyl group with (halo)alkyl esters, transacetalization with bis- (alkanoyl)acetals or condensation of the hydroxyl group with an activated aldehyde followed by acylation of the intermediate hemiacetal.

The compounds of the invention are useful for inhibiting retroviral protease, in particular HIV protease, in vitro or in vivo (especially in mammals and in particular in humans). The compounds of the present invention are also useful for the inhibition of retroviruses in vivo, especially human immunodeficiency virus (HIV). The compounds of the present invention are also useful for the treatment or prophylaxis of diseases caused by retroviruses, especially acquired immune deficiency syndrome or an HIV infection in a human or other mammal.

Total daily dose administered to a human or other mammal host in single or divided doses may be in amounts, for example, from 0.001 to 300 mg/kg body weight daily and more usually 0.1 to 10 mg. Dosage unit compositions may contain such amounts of submultiples thereof to make up the daily dose. The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.

It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination, and the severity of the particular disease undergoing therapy.

The compounds of the present invention may be administered orally, parenterally, sublingually, by inhalation spray, rectally, or topically in dosage unit formulations containing conventional nontoxic pharmaceutically acceptable carriers, adjuvants, and vehicles as desired. Topical administration may also involve the use of transdermal administration such as transdermal patches or iontophoresis devices. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscular, intrasternal injection, or infusion techniques.

Injectable preparations, for example, sterile injectable aqueous or oleagenous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1 ,3-propanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

Suppositories for rectal administration of the drug can be prepared by mixing the drug with a suitable nonirritating excipient such as cocoa butter and polyethylene glycols which are solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum and release the drug.

Solid dosage forms for oral administration may include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound may be admixed with at least one inert diluent such as sucrose lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., lubricating agents such as magnesium stearate. In the case of capsules, tablets, and pills, the dosage forms may also comprise buffering agents. Tablets and pills can additionally be prepared with enteric coatings.

Liquid dosage forms for oral administration may include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs containing inert diluents commonly used in the art, such as water. Such compositions may also comprise adjuvants, such as wetting agents, emulsifying and suspending agents, and sweetening, flavoring, and perfuming agents.

The compounds of the present invention can also be administered in the form of liposomes. As is known in the art, liposomes are generally derived from phospholipids or other lipid substances. Liposomes are formed by mono- or multi-lamellar hydrated liquid crystals that are dispersed in an aqueous medium. Any non-toxic, physiologically acceptable and metabolizable lipid capable of forming liposomes can be used. The present compositions in liposome form can contain, in addition to a compound of the present invention, stabilizers, preservatives, excipients, and the like. The preferred lipids are the phospholipids and phosphatidyl cholines (lecithins), both natural and synthetic.

Methods to form liposomes are known in the art. See, for example, Prescott, Ed., Methods in Cell Biology. Volume XIV, Academic Press, New York, N.Y. (1976), p. 33 et seq.

While the compounds of the invention can be administered as the sole active pharmaceutical agent, they can also be used in combination with one or more immunomodulators, antiviral agents, other antiinfective agents or vaccines. Other antiviral agents to be administered in combination with a compound of the present invention include AL-721 , beta interferon, polymannoacetate, reverse transcriptase inhibitors (for example, dideoxycytidine (DDC), dideoxyinosine (DDI), BCH-189, AzdU, carbovir, DDA, D4C, D4T, DP-AZT, FLT (fluorothymidine), BCH-189, 5-halo-3'-thia- dideoxycytidine, PMEA, zidovudine (AZT) and the like), non-nucleoside reverse transcriptase inhibitors (for example, R82193, L-697,661 , BI-RG-587 (nevirapine), DMP-266, and the like) retroviral protease inhibitors (for example, HIV protease inhibitors such as ritonavir, ABT-378, saquinavir, nelfinavir, indinavir, VX-478 (amprenavir), SC-52151 , KNI-227, KNI-272, U-140690, DMP- 450, and the like), HEPT compounds, L,697,639, R82150, U-87201 E and the like), TAT inhibitors (for example, RO-24-7429 and the like), trisodium phosphonoformate, HPA-23, eflonithine, Peptide T, Reticulose (nucleophosphoprotein), ansamycin LM 427, trimetrexate, UA001 , ribavirin, alpha interferon, oxetanocin, oxetanocin-G, cylobut-G, cyclobut-A, ara-M, BW882C87, foscarnet, BW256U87, BW348U87, L-693,989, BV ara-U, CMV tπ^'clonal antibodies, FIAC, HOE-602, HPMPC, MSL-109, TI-23, trifluridine, vidarabine, famciclovir, penciclovir, acyclovir, ganciclovir, castanospermine, rCD4/CD4-lgG, CD4-PE40, butyl-DNJ, hype cin, oxamyristic acid, dextran sulfate and pentosan polysulfate. Immunomodulators that can be administered in combination with a compound of the present invention include bropirimine, Ampligen, anti-human alpha interferon antibody, colony stimulting factor, CL246,738, lmreg-1 , lmreg-2, diethydithiocarbamate, interleukin-2, alpha- interferon, inosine pranobex, methionine enkephalin, muramyl-t peptide, TP-5, erythropoietin, naltrexone, tumor necrosis facator, beta interferon, gamma interferon, interleukin-3, interleukin-4, autologous CD8+ infusion, alpha interferon immunoglobulin, IGF-1 , anti-Leu-3A, autovaccination, biostimulation, extracorporeal photophoresis, FK-565, FK-506, G-CSF, GM-CSF, hyperthermia, isopinosine, IVIG, HIVIG, passive immunotherapy and polio vaccine hyperimmunization. Other antiinfective agents that can be administered in combination with a compound of the present invention include pentamidine isethionate. Any of a variety of HIV or AIDS vaccines (for example, gp120 (recombinant), Env 2-3 (gp120), HIVAC-1 e (gp120), gp160 (recombinant), VaxSyn HIV-1 (gp160), Immuno-Ag (gp160), HGP-30, HIV-lmmunogen, p24 (recombinant), VaxSyn HIV-1 (p24) can be used in combination with a compound of the present invention.

Other agents that can be used in combination with the compounds of this invention are ansamycin LM 427, apurinic acid, ABPP, AI-721 , carrisyn, AS- 101 , avarol, azimexon, colchicine, compound Q, CS-85, N-acetyl cysteine, (2- oxothiazolidine-4-carboxylate), D-penicillamine, diphenylhydantoin, EL-10, erythropoieten, fusidic acid, glucan, HPA-23, human growth hormone, hydroxchloroquine, iscador, L-ofloxacin or other quinolone antibiotics, lentinan, lithium carbonate, MM-1 , monolaurin, MTP-PE, naltrexone, neurotropin, ozone, PAI, panax ginseng, pentofylline, pentoxifylline, Peptide T, pine cone extract, polymannoacetate, reticulose, retrogen, ribavirin, bozymes, RS-47, Sdc-28, silicotungstate, THA, thymic humoral factor, thymopentin, thymosin fraction 5, thymosin alpha one, thymostimulin, UA001 , uridine, vitamin B12 and wobemugos.

Other agents that can be used in combination with the compounds of this invention are antifungals such as amphote cin B, clotrimazole, flucytosine, fluconazole, itraconazole, ketoconazole and nystatin and the like.

Other agents that can be used in combination with the compounds of this invention are antibacterials such as amikacin sulfate, azithromycin, ciprofloxacin, tosufloxacin, clarithromycin, clofazimine, ethambutol, isoniazid, pyrazinamide, rifabutin, fampin, streptomycin and TLC G-65 and the like.

Other agents that can be used in combination with the compounds of this invention are anti-neoplasties such as alpha interferon, COMP (cyclophosphamide, vincristine, methotrexate and prednisone), etoposide, mBACOD (methotrexate, bleomycin, doxorubicin, cyclophosphamide, vincristine and dexamethasone), PRO-MACE/MOPP(prednisone, methotrexate (w/leucovin rescue), doxorubicin, cyclophosphamide, etoposide/mechlorethamine, vincristine, prednisone and procarbazine), vincristine, vinblastine, angioinhibins, pentosan polysulfate, platelet factor 4 and SP-PG and the like.

Other agents that can be used in combination with the compounds of this invention are drugs for treating neurological disease such as peptide T, ritalin, lithium, elavil, phenytoin, carbamazipine, mexitetine, heparin and cytosine arabinoside and the like.

Other agents that can be used in combination with the compounds of this invention are anti-protozoals such as albendazole, azithromycin, clarithromycin, clindamycin, corticosteroids, dapsone, DIMP, eflornithine, 566C80, fansidar, furazolidone, L, 671 , 329, letrazuril, metronidazole, paromycin, pefloxacin, pentamidine, piritrexim, primaquine, pyrimethamine, somatostatin, spiramycin, sulfadiazine, trimethoprim, TMP/SMX, trimetrexate and WR 6026 and the like.

Among the preferred agents for treatment of HIV or AIDS in combination with the compounds of this invention are reverse transcriptase inhibitors and other HIV protease inhibitors. It will be understood that agents which can be combined with the compounds of the present invention for the treatment or prophylaxis of AIDS or an HIV infection are not limited to those listed above, but include in principle any agents useful for the treatment or prophylaxis of AIDS or an HIV infection.

When administered as a combination, the therapeutic agents can be formulated as separate compositions which are given at the same time or different times, or the therapeutic agents can be given as a single composition.

The foregoing is merely illustrative of the invention and is not intended to limit the invention to the disclosed compounds. Variations and changes which are obvious to one skilled in the art are intended to be within the scope and nature of the invention which are defined in the appended claims.

Claims

CLAI MS

1. A compound having formula J,:

I wherein R¹ is a thiazolyl group having the formula

and R² is a group having the formula:

wherein R³ is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and Y is CH or N;

R⁴ is -W-R⁵;

W is -0-, -S-, or -(CH₂)_n-; and R⁵ is selected from the group consisting of alkyl, and aryl; n is from 0 to 6; or R⁴ and the ring taken together can form a bicyclic group having the formula:

with the proviso that when W is O, or S then Y is CH; R⁶ is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; and

Z is -0-, -S-, -CH₂- or -N(R⁷)-; and R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, amino, alkylamino, dialkylamino and halogen; or a pharmaceutically acceptable salt, ester or prodrug thereof.

2. The compound according to claim 1 , wherein R³ is alkyl or cycloalkyl and Z is -O-, or -N(R⁷)- .

3. The compound according to claim 2, wherein R³ is alkyl.

4. The compound according to claim 2, wherein R³ is cycloalkyl selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

5. The compound according to claim 3, wherein R³ is selected from the group consisting of hydrogen, methyl, ethyl, propyl and isopropyl.

6. The compound according to claim 5, wherein R³ is isopropyl.

7. The compound according to claim 1 , wherein Z is -0-.

8. The compound according to claim 1 , wherein Z is -N(R⁷)- and R⁷ is methyl.

9. The compound according to claim 1 , wherein Y is nitrogen, W is -CH₂- and R⁵ is aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl.

10. The compound according to claim 9, wherein R⁵ is substituted with fluorine.

1 1. The compound according to claim 9, wherein R⁵ is substituted with 1 to 3 hydroxy groups.

12. The compound according to claim 9, wherein R⁵ is substituted with 1 to 3 alkoxy or alkylthio groups.

13. The compound according to claim 9, wherein R⁵ is substituted with two alkoxy groups.

14. The compound according to claim 9, wherein R⁵ is substituted with at least one hydroxy group and at least one methoxy group.

15. The compound according to claim 1 , wherein Y is CH, and R⁵ is alkyl, or aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl.

16. The compound according to claim 15, wherein R⁵ is substituted with 1 to 3 alkoxy or alkylthio groups.

17. The compound according to claim 15, wherein R⁵ is substituted with a hydroxy group.

18. The compound according to claim 15, wherein R⁵ is substituted with a methoxy group.

19. The compound according to claim 15, wherein W is -0-.

20. The compound according to claim 19, wherein R⁵ is methyl substituted with a thiazolyl group.

21. The compound according to claim 1 , wherein R⁶ is selected from the group consisting of alkyl, hydroxyalkyl, and cycloalkyl.

22. The compound according to claim 21 , wherein R⁶ is lower alkyl selected from the group consisting of methyl, ethyl, propyl, or butyl.

23. The compound according to claim 22, wherein R⁶ is terf-butyl or hy- droxybutyl.

24. A compound having formula U:

I I

wherein R¹ is a thiazolyl group having the formula

and R² is a group having the formula:

wherein R³ is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and

X is -C(0)- or -S(0)₂-;

R⁸ is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl.or (heteroaryl)alkyl;

R⁹ is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; and Z is -0-, -S-, -CH₂- or -N(R⁷)- wherein R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen; or a pharmaceutically acceptable salt, ester or prodrug thereof.

25. The compound according to claim 24, wherein R³ is alkyl or cycloalkyl and Z is -0-, or -N(R⁷)-.

26. The compound according to claim 25, wherein R³ is alkyl.

27. The compound according to claim 25, wherein R³ is cycloalkyl selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

28. The compound according to claim 26, wherein R³ is selected from the group consisting of hydrogen, methyl, ethyl, or propyl.

29. The compound according to claim 27, wherein R³ is isopropyl.

30. The compound according to claim 25, wherein Z is -0-.

31. The compound according to claim 25, wherein Z is -N(R⁷)- and R⁷ is methyl.

32. The compound according to claim 24, wherein R⁸ is selected from the group consisting of alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, or heteroaryl.

33. The compound according to claim 24, wherein X is -S(0)₂-; R⁸ is aryl selected from the group consisting of phenyl, and heteroaryl; and R⁹ is lower alkyl.

34. The compound according to claim 24, wherein R⁹ is selected from the group consisting of /sopropyl, /sobutyl, or 3-methyl-1 -butyl.

35. The compound according to claim 34, wherein R⁹ is /so-butyl.

36. The compound according to claim 32, wherein R⁸ is substituted with 1 to 3 amino groups.

37. The compound selected from the group consisting of :

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-4-(1 ,3- benzodioxol-5-ylmethyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)- 2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-(phenylmethyl)-1 -piperazinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl-(1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-((4-fluorophenyl)methyl)-1 -piperazinyl)-2-hy- droxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-(5-thienylmethyl)-1 -piperazinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-(4-(3-hydroxyphenyl)methyl)-1 -piperazinyl)-2- hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-(3-pyridinylmethyl)-1 -piperazinyl)-2-hydroxy- 1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-(4-pyridinylmethyl)-1-piperazinyl)-2-hydroxy- 1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1-methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-((4-hydroxyphenyl)methyl)-1 -piperazinyl)-2- hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1 -methylethyl)-4-thiazolylmethyl-(1 S)-1 -((((1 S,2R)-3-((2S)-4-(1 H- benzimidiazol-2-ylmethyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazin- yl)-2-hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbam- ate;

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 - dimethylethyl)amino)carbonyl)-4-(2-quinolinylmethyl)-1 -piperazinyl)-2-hydroxy- 1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl ((1 S)-1 -((((1 S,2R)-3-((2S)-4-((3,4-di- methoxylphenyl)methyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)- 2-hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

N'-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-4- (5-thiazolylmethyl)-1 -piperazinyl)-2-hydroxy-1 -(phenylmethyl)propyl)amino)car- bonyl)-2-methylpropyl)-N-methyl-N-(2-(1 -methylethyl)-4-thiazolylmethyl)urea;

N'-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-4- (phenylmethyl)-1 -piperazinyl)-2-hydroxy-1 -(phenylmethyl)propyl)amino)car- bonyl)-2-methylpropyl)-N-methyl-N-(2-(1 -methylethyl)-4-thiazolylmethyl)urea;

N'-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-4- ((4-hydroxy-3-methoxyphenyl)methyl)-1 -piperazinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1 - methylethyl)-4-thiazolylmethyl)urea;

2-methyl-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-4-(1 ,3-benzodi- oxol-5-ylmethyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)-2-hy- droxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-methyl-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-4-((3,4-dimethoxyl- phenyl)methyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)-2-hy- droxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-methyl-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethyl- ethyl)amino)carbonyl)-4-((4-fluorophenyl)methyl)-1 -piperazinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-ethyl-4-thiazolylmethyl-(1 S)-1 -((((1 S,2R)-3-((2S)-4-((3,4-dimethoxyl- phenyl)methyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)-2-hy- droxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1 -methylethyl)-5-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-4-((3,4-di- methoxylphenyl)methyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)- 2-hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-ethyl-5-thiazolylmethyl-(1 S)-1 -((((1 S,2R)-3-((2S)-4-((3,4-dimethoxyl- phenyl)methyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)-2-hy- droxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-methyl-5-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-4-((3,4-dimethoxyl- phenyl)methyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)-2-hy- droxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

N'-((1 R)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-4- (5-thiazolylmethyl)-1 -piperazinyl)-2-hydroxy-1 -(phenylmethyl)propyl)amino)car- bonyl)-2-methylpropyl)-N-methyl-N-(5-thiazolylmethyl)urea;

N'-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethyiethyl)amino)carbonyl)-4- (5-thiazolylmethyl)-1 -piperazinyl)-2-hydroxy-1 -(phenylmethyl)propyl)amino)car- bonyl)-2-methylpropyl)-N-methyl-N-(5-thiazolylmethyl)urea;

5-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethyl- ethyl)amino)carbonyl)-4-(phenylmethyl)-1 -piperazinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

5-thiazolylmethyl-(1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -dimethyl- ethyl)amino)carbonyl)-4-(5-thiazolylmethyl)-1 -piperazinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

N'-((1 S)-1 -((((1 S,2R)-3-((((1 ,1 -dimethylethyl)amino)carbonyl)(2-methyl- ethyl)propylamino)-2-hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2- methylpropyl)-N-methyl-N-(2-(1 -methylethyl)-4-thiazolylmethyl)urea;

2-(1 -methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((((1 ,1 -dimethyl- ethyl)amino)carbonyl)(2-methylethyl)propylamino)-2-hydroxy-1 -(phenylmethyl)- propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -methylethyl)-4-thiazolylmethyl-(1 S)-1 -((((1 S,2R)-3-(((4- aminophenyl)sulfonyl)(1 -methylethyl)amino)-2-hydroxy-1 -(phenylmethyl)- propyl)amino)carbonyl)-2-methylpropyl)carbamate;

N'-((1 S)-1 -((((1 S,2R)-3-(((4-amino╧ühenyl)sulfonyl)(1 -methylethyl)amino)- 2-hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl- N-(2-(1 -methylethyl)-4-thiazolylmethyl)urea; 2-0 -methylethyl)-5-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((3S)-3-(((1 ,1 - dimethylethyl)amino)carbonyl)(4a╬▒,8a╬▒)octahydro-2-isoquinolinyl)-2-hydroxy- 1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

N'-((1 S)-1 -((((1 S,2R)-3-((3S)-3-(((1 ,1 -dimethyl- ethyl)amino)carbonyl)(4a╬▒,8a╬▒)octahydro-2-isoquinolinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1 - methylethyl)-5-thiazolylmethyl)urea,

N'-((1 S)-1 -((((1 S,2R)-3-((2S,4R)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)- 4-(5-thiazolylmethoxy)-1-piperidinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)- carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1 -methylethyl)-4-thiazolylmethyl)- urea;

2-(1 -methylethyl)-4-thiazolylmethyl ((1 S)-1 -((((1 S,2R)-3-((2S,4R)-2- (((1 ,1 -dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethoxy)-1 -piperidinyl)-2-hy- droxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

S-(2-(1 -methylethyl)-4-thiazolylmethyl) ((1 S)-1 -((((1 S,2R)-3-((2S)-4-(1 ,3- benzodioxol-5-ylmethyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)- 2-hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)- carbamothioate; and

4-(1 ,3-benzodioxol-5-ylmethyl)-N-(1 ,1 -dimethylethyl)-1 -((2R,3S)-3-

(((2S)-2-((3-(2-(1 -methylethyl)-4-thiazolyl)-1 -oxopropyl)amino)-3-methyl-1 -oxo- butyl)amino)-2-hydroxy-4-phenylbutyl)-2-piperazinecarboxamide; or a pharmaceutically acceptable salt, ester or prodrug thereof.

38. The compound according to claim 37, selected from the group consisting of :

2-0 -Methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-4-(1 ,3- benzodioxol-5-ylmethyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)- 2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate;

2-(1 -Methylethyl)-4-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((2S)-2-(((1 ,1 -di- methylethyl)amino)carbonyl)-4-(5-thienylmethyl)-1 -piperazinyl)-2-hydroxy-1 - (phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1 -Methylethyl)-4-thiazolylmethyl ((1 S)-1 -((((1 S,2R)-3-((2S)-4-((3,4-di- methoxylphenyl)methyl)-2-(((1 ,1 -dimethylethyl)amino)carbonyl)-1 -piperazinyl)- 2-hydroxy-1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate

N'-((1 S)-1 -((((1 S,2R)-3-((2S,4R)-2-(((1 ,1 -Dimethylethyl)amino)carbonyl)- 4-(5-thiazolylmethoxy)-1 -piperidinyl)-2-hydroxy-1 -(phenylmethyl)propyl)amino)- carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1 -methylethyl)-4- thiazolylmethyl)urea; and

2-(1 -Methylethyl)-5-thiazolylmethyl-((1 S)-1 -((((1 S,2R)-3-((3S)-3-(((1 ,1 - dimethylethyl)amino)carbonyl)(4a╬▒,8a╬▒)octahydro-2-isoquinolinyl)-2-hydroxy- 1 -(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; or a pharmaceutically acceptable salt, ester or prodrug thereof.

39. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of Claim 1.

40. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of Claim 24.

41. A method for inhibiting HIV protease comprising administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Claim 1.

42. A method for treating an HIV infection comprising administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Claim 24.

43. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of Claim 9.

44. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of Claim 20.

45. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of Claim 36.

46. A process for the preparation of a compound of the formula i:

I wherein R¹ is a thiazolyl group having the formula

and R² is a group having the formula:

wherein R³ is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and Y is CH or N; p4 _is - -R5;

with the proviso that when W is O, or S then Y is CH;

R⁶ is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; and

Z is -0-, -S-, -CH₂- or -N(R⁷)-; and R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen.

47. A process for the preparation of a compound of the formula _ \[

I I wherein R¹ is a thiazolyl group having the formula

and R² is a group having the formula:

^XN^R⁸ R* wherein R³ is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and X is -C(O)- or -S(0)2-; R⁸ is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl;

R⁹ is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; and

Z is -0-, -S-, -CH₂- or -N(R⁷)- wherein R⁷ is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen.