WO1999058712A3 - Nachweis von genen - Google Patents
Nachweis von genen Download PDFInfo
- Publication number
- WO1999058712A3 WO1999058712A3 PCT/DE1999/001423 DE9901423W WO9958712A3 WO 1999058712 A3 WO1999058712 A3 WO 1999058712A3 DE 9901423 W DE9901423 W DE 9901423W WO 9958712 A3 WO9958712 A3 WO 9958712A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- genes
- detection
- dna
- amplified
- gene
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Die vorliegende Erfindung betrifft ein Verfahren zum Nachweis von Genen und ihrer Expression, bei dem eine DNA mit für Gene spezifischen Primern amplifiziert und die amplifizierte DNA zur Hybridisierung mit einer DNA-Bibliothek verwendet wird.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19820982A DE19820982A1 (de) | 1998-05-12 | 1998-05-12 | Nachweis von Genen |
DE19820982.7 | 1998-05-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1999058712A2 WO1999058712A2 (de) | 1999-11-18 |
WO1999058712A3 true WO1999058712A3 (de) | 2000-03-09 |
Family
ID=7867350
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DE1999/001423 WO1999058712A2 (de) | 1998-05-12 | 1999-05-11 | Nachweis von genen |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE19820982A1 (de) |
WO (1) | WO1999058712A2 (de) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995008647A1 (en) * | 1993-09-24 | 1995-03-30 | The Trustees Of Columbia University In The City Of New York | METHOD FOR CONSTRUCTION OF NORMALIZED cDNA LIBRARIES |
US5512463A (en) * | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
WO1997035026A1 (en) * | 1996-03-18 | 1997-09-25 | Molecular Biology Resources, Inc. | Target nucleic acid sequence amplification |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1992000385A1 (en) * | 1990-06-29 | 1992-01-09 | Fox Chase Cancer Center | Universal mapping probes for identifying and mapping conserved dna sequences |
US5518901A (en) * | 1993-04-19 | 1996-05-21 | Murtagh; James J. | Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease |
-
1998
- 1998-05-12 DE DE19820982A patent/DE19820982A1/de not_active Withdrawn
-
1999
- 1999-05-11 WO PCT/DE1999/001423 patent/WO1999058712A2/de active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5512463A (en) * | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
WO1995008647A1 (en) * | 1993-09-24 | 1995-03-30 | The Trustees Of Columbia University In The City Of New York | METHOD FOR CONSTRUCTION OF NORMALIZED cDNA LIBRARIES |
WO1997035026A1 (en) * | 1996-03-18 | 1997-09-25 | Molecular Biology Resources, Inc. | Target nucleic acid sequence amplification |
Non-Patent Citations (4)
Title |
---|
CROSS S H ET AL: "PURIFICATION OF CPG ISLANDS USING A METHYLATED DNA BINDING COLUMN", NATURE GENETICS,US,NEW YORK, NY, vol. 6, no. 3, pages 236-244, XP002911234, ISSN: 1061-4036 * |
HAYASHIAKI Y E AL.: "A new method for constructing NotI linking and boundary libraries using a restriction trapper", GENOMICS, vol. 14, 1992, pages 733 - 739, XP000857984 * |
JUNG V ET AL.: "Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites", NUCLEIC ACID RESEARCH, vol. 18, no. 20, 1990, pages 6156, XP002127829 * |
PERGOLIZZI R ET AL: "Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 168, no. 2, pages 267-270, XP004042973, ISSN: 0378-1119 * |
Also Published As
Publication number | Publication date |
---|---|
WO1999058712A2 (de) | 1999-11-18 |
DE19820982A1 (de) | 1999-11-18 |
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