WO1999055829A3 - Process for preparing doxorubicin - Google Patents

Process for preparing doxorubicin Download PDF

Info

Publication number
WO1999055829A3
WO1999055829A3 PCT/US1999/007016 US9907016W WO9955829A3 WO 1999055829 A3 WO1999055829 A3 WO 1999055829A3 US 9907016 W US9907016 W US 9907016W WO 9955829 A3 WO9955829 A3 WO 9955829A3
Authority
WO
WIPO (PCT)
Prior art keywords
daunorubicin
doxorubicin
fragment containing
gene
dna region
Prior art date
Application number
PCT/US1999/007016
Other languages
French (fr)
Other versions
WO1999055829A2 (en
Inventor
Augusto Inventi Solari
Giovanna Zanuso
Silvia Filippini
Francesca Torti
Sharee Otten
Anna Luisa Colombo
Charles R Hutchinson
Original Assignee
Pharmacia & Upjohn Spa
Augusto Inventi Solari
Giovanna Zanuso
Silvia Filippini
Francesca Torti
Sharee Otten
Anna Luisa Colombo
Charles R Hutchinson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia & Upjohn Spa, Augusto Inventi Solari, Giovanna Zanuso, Silvia Filippini, Francesca Torti, Sharee Otten, Anna Luisa Colombo, Charles R Hutchinson filed Critical Pharmacia & Upjohn Spa
Priority to CA002326500A priority Critical patent/CA2326500A1/en
Priority to EP99919767A priority patent/EP1071746A4/en
Priority to JP2000545975A priority patent/JP2002512784A/en
Publication of WO1999055829A2 publication Critical patent/WO1999055829A2/en
Publication of WO1999055829A3 publication Critical patent/WO1999055829A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The ability to convert daunorubicin into doxorubicin can be improved by transforming a host cell with a recombinant vector comprising a DNA molecule comprising: a DNA region or fragment containing the gene doxA encoding daunorubicin 14-hydroxylase and a DNA region or fragment containing one or more gene conferring daunorubicin and doxorubicin resistance.
PCT/US1999/007016 1998-04-24 1999-04-22 Process for preparing doxorubicin WO1999055829A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002326500A CA2326500A1 (en) 1998-04-24 1999-04-22 Process for preparing doxorubicin
EP99919767A EP1071746A4 (en) 1998-04-24 1999-04-22 Process for preparing doxorubicin
JP2000545975A JP2002512784A (en) 1998-04-24 1999-04-22 Method for producing doxorubicin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6560698A 1998-04-24 1998-04-24
US09/065,606 1998-04-24

Publications (2)

Publication Number Publication Date
WO1999055829A2 WO1999055829A2 (en) 1999-11-04
WO1999055829A3 true WO1999055829A3 (en) 1999-12-23

Family

ID=22063870

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/007016 WO1999055829A2 (en) 1998-04-24 1999-04-22 Process for preparing doxorubicin

Country Status (5)

Country Link
EP (1) EP1071746A4 (en)
JP (1) JP2002512784A (en)
CN (1) CN1198930C (en)
CA (1) CA2326500A1 (en)
WO (1) WO1999055829A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006111561A1 (en) * 2005-04-21 2006-10-26 Dsm Ip Assets B.V. Improved microbial production of anthracyclins
CN1962869B (en) * 2005-11-09 2010-08-11 上海医药工业研究院 Regulation protein SnpR and its gene and application
CN101016533B (en) * 2005-11-09 2010-05-19 上海医药工业研究院 Engineering bacterium capable of producing anthracene ring antibiotics and application of the same
US8383392B2 (en) 2007-09-14 2013-02-26 Meiji Seika Pharma Co., Ltd. Transformant and method for production of non-natural antibiotic
CN102229906B (en) * 2011-03-09 2013-04-10 北京赛诺百奥生物技术有限公司 Daunorubicin and adriamycin producing engineered pseudomonas
CN104059892A (en) * 2013-03-18 2014-09-24 江苏禾昌生物科技有限公司 Daunorubicin C-14 hydroxylase mutant and production method of genetically engineered bacteria thereof
CN111187746B (en) * 2018-11-15 2022-11-22 上海医药工业研究院 Genetically engineered bacterium and preparation method and application thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5364781A (en) * 1991-11-18 1994-11-15 Farmitalia Carlo Erba S.R.L Process for preparing daunorubicin
WO1997006266A1 (en) * 1995-08-03 1997-02-20 Abbott Laboratories Vectors and process for producing high purity 6,12-dideoxyerythromycin a by fermentation
US5652125A (en) * 1996-06-10 1997-07-29 Pharmacia S.P.A. Process for preparing daunorubicin
US5665564A (en) * 1988-05-27 1997-09-09 Pharmacia & Upjohn S.P.A. Isolation and characterisation of genes resistant to anthracycline antibiotics
WO1997044439A2 (en) * 1996-05-24 1997-11-27 The Ohio State University Research Foundation Method of producing doxorubicin
US5695966A (en) * 1995-02-27 1997-12-09 Pharmacia & Upjohn S.P.A. DNA encoding daunorubicin 14-hyroxylase and method for preparing doxorubicin

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665564A (en) * 1988-05-27 1997-09-09 Pharmacia & Upjohn S.P.A. Isolation and characterisation of genes resistant to anthracycline antibiotics
US5364781A (en) * 1991-11-18 1994-11-15 Farmitalia Carlo Erba S.R.L Process for preparing daunorubicin
US5695966A (en) * 1995-02-27 1997-12-09 Pharmacia & Upjohn S.P.A. DNA encoding daunorubicin 14-hyroxylase and method for preparing doxorubicin
WO1997006266A1 (en) * 1995-08-03 1997-02-20 Abbott Laboratories Vectors and process for producing high purity 6,12-dideoxyerythromycin a by fermentation
WO1997044439A2 (en) * 1996-05-24 1997-11-27 The Ohio State University Research Foundation Method of producing doxorubicin
US5652125A (en) * 1996-06-10 1997-07-29 Pharmacia S.P.A. Process for preparing daunorubicin

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHEOL KYU HWANG, ET AL.: "EXPRESSION OF STREPTOMYCES PEUCETIUS GENES FOR DOXORUBICIN RESISTANCE AND AKLAVINONE 11-HYDROXYLASE IN STREPTOMYCES GALILAEUS ATCC 31133 AND PRODUCTION OF A HYBRID ACLACINOMYCIN", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 39, no. 07, 1 July 1995 (1995-07-01), US, pages 1616 - 1620, XP002932382, ISSN: 0066-4804 *
DICKENS M L, STROHL W R: "ISOLATION AND CHARACTERIZATION OF A GENE FROM STREPTOMYCES SP. STRAIN C5 THAT CONFERS THE ABILITY TO CONVERT DAUNOMYCIN TO DOXORUBICIN ON STREPTOMYCES LIVIDANS TK24", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 178, no. 11, 1 June 1996 (1996-06-01), US, pages 3389 - 3395, XP002932381, ISSN: 0021-9193 *
LOMOVSKAYA N, ET AL.: "THE STREPTOMYCES PEUCETIUS DRRC GENE ENCODES A UVRA-LIKE PROTEIN INVOLVED IN DAUNORUBICIN RESISTANCE AND PRODUCTION", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 178, no. 11, 1 June 1996 (1996-06-01), US, pages 3238 - 3245, XP002932384, ISSN: 0021-9193 *
PARJIT KAUR: "EXPRESSION AND CHARACTERIZATION OF DRRA AND DRRB PROTEINS OF STREPTOMYCES PEUCETIUS IN ESCHERICHIA COLI: DRRA IS AN ATP BINDING PROTEIN", JOURNAL OF BACTERIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 179, no. 03, 1 February 1997 (1997-02-01), US, pages 569 - 575, XP002932383, ISSN: 0021-9193 *
See also references of EP1071746A4 *

Also Published As

Publication number Publication date
CA2326500A1 (en) 1999-11-04
JP2002512784A (en) 2002-05-08
WO1999055829A2 (en) 1999-11-04
EP1071746A2 (en) 2001-01-31
EP1071746A4 (en) 2003-04-23
CN1298453A (en) 2001-06-06
CN1198930C (en) 2005-04-27

Similar Documents

Publication Publication Date Title
EP1131333A4 (en) ISOLATED NUCLEIC ACID MOLECULES WHICH ENCODE T CELL INDUCIBLE FACTORS (TIFs), THE PROTEINS ENCODED, AND USES THEREOF
WO2000008057A3 (en) Molecules designated b7l-1
EP1159441B8 (en) Delivery of nucleic acids and proteins to cells
WO2000018914A3 (en) Novel dkr polypeptides
WO2000026349A3 (en) Recombinant oleandolide polyketide synthase
AU8095594A (en) Dna encoding triol polyketide synthase
NZ510356A (en) Molecules designated LDCAM
EP1522579A3 (en) Hyaluronan synthase gene and uses thereof
WO1998021343A8 (en) Genes encoding telomerase proteins
WO1999055829A3 (en) Process for preparing doxorubicin
IL140439A0 (en) Polyketides, their preparation and materials for use therein
AU2462399A (en) Cubilin protein, dna sequences encoding cubilin and uses thereof
AU2667995A (en) Chimerical peptide-nucleic acid fragment, process for producing the same and its use for appropriately introducing nucleic acids into cell organelles and cells
AU6181494A (en) DNA fragment, vector containing the DNA fragment, transformant transformed with the vector and process for producing protein using the vector
AU2409397A (en) A prokaryotic protein having functional and structural homology with the uman p-glycoprotein encoded by the mdr-1 gene, nucleic acids encoding and cells expressing said protein
HUT69768A (en) Process for preparation of daunorubicin
AU4707500A (en) Mutations in nucleic acid molecules encoding 11-cis retinol dehydrogenase, the mutated proteins, and uses thereof
AU1183200A (en) Novel gene encoding brain-specific membrane protein
WO2000040607A3 (en) Tiam2 (t-cell lymphoma invasion and metastasis 2) nucleotide exchange factor
ZA99445B (en) Isolated, polypeptides which bind to HLA-A29 molecules nucleic acid, the molecules encoding these, and uses thereof.
AU5245399A (en) T cell receptor proteins, nucleic acid molecules, and uses thereof
MX9605018A (en) Process for preparing doxorubicin.
AU2001253804A1 (en) Isolated human transporter proteins, nucleic acid molecules encoding the same, and uses thereof
AU1323200A (en) Isolated nucleic acid molecules which encode scp proteins, and uses thereof
AU2001257287A1 (en) Isolated human transporter proteins, nucleic acid molecules encoding human transporter proteins, and uses thereof

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99805400.3

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): CA CN JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

AK Designated states

Kind code of ref document: A3

Designated state(s): CA CN JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2000 545975

Country of ref document: JP

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2326500

Country of ref document: CA

Ref document number: 2326500

Country of ref document: CA

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 1999919767

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09673254

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1999919767

Country of ref document: EP