WO1999053097A3 - Method for developing degenerate pcr primers - Google Patents

Method for developing degenerate pcr primers Download PDF

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Publication number
WO1999053097A3
WO1999053097A3 PCT/US1999/007513 US9907513W WO9953097A3 WO 1999053097 A3 WO1999053097 A3 WO 1999053097A3 US 9907513 W US9907513 W US 9907513W WO 9953097 A3 WO9953097 A3 WO 9953097A3
Authority
WO
WIPO (PCT)
Prior art keywords
acid sequence
gene
amplification
degenerate primers
developing
Prior art date
Application number
PCT/US1999/007513
Other languages
French (fr)
Other versions
WO1999053097A2 (en
Inventor
David R Kilpatrick
Original Assignee
Us Health
David R Kilpatrick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health, David R Kilpatrick filed Critical Us Health
Priority to AU33837/99A priority Critical patent/AU3383799A/en
Publication of WO1999053097A2 publication Critical patent/WO1999053097A2/en
Publication of WO1999053097A3 publication Critical patent/WO1999053097A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a method for designing and making degenerate primers for use in amplification assays. Using the present method, degenerate primers for the amplification and subsequent detection of virtually all genes that encode an amino acid sequence can be obtained. The degenerate primers are effective for detection of any gene which lies within a coding region that results in the production of a protein. Examples of genes that can be detected include those where the sequence of the specific target gene is known or unknown and where the amino acid sequence encoded for by the gene is structural, nonstructural, or enzymatic. The method provides highly specific primers which are effective for substantial amplification of a target sequence even where the target nucleic acid sequence is unknown.
PCT/US1999/007513 1998-04-15 1999-04-06 Method for developing degenerate pcr primers WO1999053097A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU33837/99A AU3383799A (en) 1998-04-15 1999-04-06 Method for developing degenerate pcr primers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8194498P 1998-04-15 1998-04-15
US60/081,944 1998-04-15

Publications (2)

Publication Number Publication Date
WO1999053097A2 WO1999053097A2 (en) 1999-10-21
WO1999053097A3 true WO1999053097A3 (en) 1999-11-18

Family

ID=22167406

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/007513 WO1999053097A2 (en) 1998-04-15 1999-04-06 Method for developing degenerate pcr primers

Country Status (2)

Country Link
AU (1) AU3383799A (en)
WO (1) WO1999053097A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6846621B1 (en) 1999-03-31 2005-01-25 The United States Of America As Represented By The Department Of Health And Human Services Typing of human enteroviruses
CN102534036B (en) * 2012-02-24 2013-09-18 河南科技大学 Taq DNA (deoxyribonucleic acid) polymerase cold start activity detection method
US9938588B2 (en) 2015-06-05 2018-04-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Compositions and methods for detecting Enterovirus D68
CN108342509B (en) * 2018-02-08 2021-07-09 北京宏微特斯生物科技有限公司 Method for enriching vertebrate viral nucleic acids

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268465A (en) * 1991-01-18 1993-12-07 The Johns Hopkins University Purification and molecular cloning of nitric oxide synthase
WO1994003644A1 (en) * 1992-08-10 1994-02-17 Cambridge Neuroscience, Inc. Inhibitors of cell proliferation, their preparation and use
WO1995002704A2 (en) * 1993-07-13 1995-01-26 THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTEMENT OF HEALTH AND HUMAN SERVICES Poliovirus specific primers and methods of detection utilizing the same
DE4338817A1 (en) * 1993-11-13 1995-06-14 Max Delbrueck Centrum Gene sequences for cellular X binding proteins isolated from mouse
EP0712933A2 (en) * 1994-10-18 1996-05-22 New England Biolabs, Inc. Isolated DNA encoding the FseI restriction endonuclease and related methods for producing the same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268465A (en) * 1991-01-18 1993-12-07 The Johns Hopkins University Purification and molecular cloning of nitric oxide synthase
WO1994003644A1 (en) * 1992-08-10 1994-02-17 Cambridge Neuroscience, Inc. Inhibitors of cell proliferation, their preparation and use
WO1995002704A2 (en) * 1993-07-13 1995-01-26 THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTEMENT OF HEALTH AND HUMAN SERVICES Poliovirus specific primers and methods of detection utilizing the same
DE4338817A1 (en) * 1993-11-13 1995-06-14 Max Delbrueck Centrum Gene sequences for cellular X binding proteins isolated from mouse
EP0712933A2 (en) * 1994-10-18 1996-05-22 New England Biolabs, Inc. Isolated DNA encoding the FseI restriction endonuclease and related methods for producing the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FORDHAM ET AL.: "Synthesis of saporin gene probes from partial sequence data", MOL. GEN. GENET., vol. 221, 1990, pages 134 - 138, XP002116639 *
PATIL R V ET AL: "PCR AMPLIFICATION OF AN ESCHERICHIA COLI GENE USING MIXED PRIMERS CONTAINING DEOXYINOSINE AT AMBIGUOUS POSITIONS IN DEGENERATE AMINO ACID CODONS", NUCLEIC ACIDS RESEARCH, vol. 18, no. 10, 25 May 1990 (1990-05-25), pages 3020, XP000310539, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO1999053097A2 (en) 1999-10-21
AU3383799A (en) 1999-11-01

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