WO1999047934A3 - Early pregnancy diagnosis using pags (pregnancy-associated glycoproteins) - Google Patents

Early pregnancy diagnosis using pags (pregnancy-associated glycoproteins) Download PDF

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Publication number
WO1999047934A3
WO1999047934A3 PCT/US1999/006038 US9906038W WO9947934A3 WO 1999047934 A3 WO1999047934 A3 WO 1999047934A3 US 9906038 W US9906038 W US 9906038W WO 9947934 A3 WO9947934 A3 WO 9947934A3
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WO
WIPO (PCT)
Prior art keywords
pregnancy
expressed
pags
early
genes
Prior art date
Application number
PCT/US1999/006038
Other languages
French (fr)
Other versions
WO1999047934A2 (en
Inventor
Robert Michael Roberts
Jonathan Andrew Green
Sancai Xie
Original Assignee
Univ Missouri
Robert Michael Roberts
Jonathan Andrew Green
Sancai Xie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Missouri, Robert Michael Roberts, Jonathan Andrew Green, Sancai Xie filed Critical Univ Missouri
Priority to AU31028/99A priority Critical patent/AU768018C/en
Priority to CA2323812A priority patent/CA2323812C/en
Priority to NZ507033A priority patent/NZ507033A/en
Priority to EP99912715A priority patent/EP1141727A2/en
Publication of WO1999047934A2 publication Critical patent/WO1999047934A2/en
Publication of WO1999047934A3 publication Critical patent/WO1999047934A3/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/471—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Gynecology & Obstetrics (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Pregnancy-associated glycoproteins (PAGs) are structurally related to the pepsins, thought to be restricted to the hoofed (ungulate) mammals and characterized by being expressed specifically in the outer epithelial cell layer (chorion/trophectoderm) of the placenta. By cloning expressed genes from ovine and bovine placental cDNA libraries, the inventors estimate that cattle, sheep, and most probably all ruminant Artiodactyla, possess possibly 100 or more PAG genes, many of which are placentally expressed. The PAGs are highly diverse in sequence, with regions of hypervariability confined largely to surface-exposed loops. Selected PAG that are products of the invasive binucleate cells, expressed highly in early pregnancy at the time of trophoblast invasion and expressed weakly, if at all, in late gestation are useful in the early diagnosis of pregnancy. In a preferred embodiment, the invention relates to immunoassays for detecting these PAGs.
PCT/US1999/006038 1998-03-20 1999-03-19 Early pregnancy diagnosis using pags (pregnancy-associated glycoproteins) WO1999047934A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU31028/99A AU768018C (en) 1998-03-20 1999-03-19 Early pregnancy diagnosis using PAGS (pregnancy- associated glycoproteins)
CA2323812A CA2323812C (en) 1998-03-20 1999-03-19 Early pregnancy diagnosis using pags (pregnancy-associated glycoproteins)
NZ507033A NZ507033A (en) 1998-03-20 1999-03-19 Compositions and methods for early pregnancy diagnosis
EP99912715A EP1141727A2 (en) 1998-03-20 1999-03-19 Early pregnancy diagnosis using pags (pregnancy-associated glycoproteins)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US7878398P 1998-03-20 1998-03-20
US60/078,783 1998-03-20
US10618898P 1998-10-28 1998-10-28
US60/106,188 1998-10-28

Publications (2)

Publication Number Publication Date
WO1999047934A2 WO1999047934A2 (en) 1999-09-23
WO1999047934A3 true WO1999047934A3 (en) 2001-07-19

Family

ID=26760935

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/006038 WO1999047934A2 (en) 1998-03-20 1999-03-19 Early pregnancy diagnosis using pags (pregnancy-associated glycoproteins)

Country Status (7)

Country Link
US (4) US6869770B1 (en)
EP (2) EP2295978A3 (en)
AR (3) AR015549A1 (en)
AU (1) AU768018C (en)
CA (1) CA2323812C (en)
NZ (1) NZ507033A (en)
WO (1) WO1999047934A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6869770B1 (en) 1998-03-20 2005-03-22 The Curators Of The University Of Missouri Compositions and methods for early pregnancy diagnosis
WO2002103352A1 (en) * 2001-06-19 2002-12-27 Idaho Research Foundation Determination of pregnancy status
US7393696B2 (en) 2001-09-28 2008-07-01 Aspenbio Pharma, Inc. Bovine pregnancy test
MXPA04004880A (en) 2001-11-20 2004-09-03 Monsanto Technology Llc Compositions and methods for accurate early pregnancy diagnosis.
US7842513B2 (en) 2002-05-02 2010-11-30 Aspenbio Pharma, Inc. Pregnancy detection
US7604950B2 (en) 2004-04-29 2009-10-20 Monsanto Technology Llc Methods for early detection of pregnancy in cows
US20080026384A1 (en) * 2005-09-30 2008-01-31 Hansen Thomas R Methods and compositions for the detection of bovine pregnancy
BRPI0820885B8 (en) * 2007-12-13 2021-03-02 Monsanto Technolgoy Llc "antibody or fragment thereof, hybridoma cell, polynucleotide, polypeptide comprising an antibody heavy or light chain domain, method for detecting pregnancy in a bovine animal, kit and method for purifying a major antigen associated with pregnancy (pag)" .
WO2009126889A1 (en) * 2008-04-10 2009-10-15 The Curators Of The University Of Missouri Assays for detecting pregnancy- associated glycoproteins
US8541187B2 (en) 2011-03-01 2013-09-24 Bio Tracking, LLC Systems and methods for detecting animal pregnancy
US20190002978A1 (en) * 2016-01-11 2019-01-03 University Of Tennessee Research Foundation Pregnancy associated glycoprotein (pag) genes as markers of bull fertility
KR102189893B1 (en) * 2017-11-15 2020-12-11 (주)에스엘에스바이오 Antibody specifically binding a bPAG1 and use thereof
CN111596073B (en) * 2020-06-04 2023-05-02 昆明天沃生物科技有限公司 Method for detecting early pregnancy of sheep
CN113960025A (en) * 2021-10-12 2022-01-21 北京农学院 Detection method for early pregnancy of dairy cow
KR20230065047A (en) * 2021-11-04 2023-05-11 주식회사 나노바이오라이프 Development of a bovine pregnancy diagnostic antibody and an early pregnancy diagnostic kit using the antibody
CN114134123B (en) * 2021-12-02 2023-05-05 深圳市爱医生物科技有限公司 Monoclonal antibody of pregnancy related glycoprotein and application thereof in early pregnancy detection of cattle
CN114047336B (en) * 2021-12-13 2024-10-29 大连医科大学 Biomarker for auxiliary diagnosis of recurrent abortion and application thereof

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Also Published As

Publication number Publication date
CA2323812C (en) 2011-05-10
AR015549A1 (en) 2001-05-02
AR075511A2 (en) 2011-04-06
US20100331206A1 (en) 2010-12-30
EP1141727A2 (en) 2001-10-10
AU3102899A (en) 1999-10-11
US6869770B1 (en) 2005-03-22
AU768018B2 (en) 2003-11-27
EP2295978A3 (en) 2012-06-06
US7763432B2 (en) 2010-07-27
EP2295978A2 (en) 2011-03-16
US20050100975A1 (en) 2005-05-12
CA2323812A1 (en) 1999-09-23
AR063820A2 (en) 2009-02-18
NZ507033A (en) 2004-06-25
WO1999047934A2 (en) 1999-09-23
US8182999B2 (en) 2012-05-22
US20070166773A1 (en) 2007-07-19
AU768018C (en) 2004-09-30

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