WO1999045120A3 - Attenuation de bacteries: matieres et methodes associees - Google Patents

Attenuation de bacteries: matieres et methodes associees Download PDF

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Publication number
WO1999045120A3
WO1999045120A3 PCT/GB1999/000675 GB9900675W WO9945120A3 WO 1999045120 A3 WO1999045120 A3 WO 1999045120A3 GB 9900675 W GB9900675 W GB 9900675W WO 9945120 A3 WO9945120 A3 WO 9945120A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
attenuated microorganisms
bacteria
sopa
microorganism
Prior art date
Application number
PCT/GB1999/000675
Other languages
English (en)
Other versions
WO1999045120A2 (fr
Inventor
Timothy Stephen Wallis
Edouard Eugenievich Galyov
Michael William Wood
Original Assignee
Biotech & Biolog Scien Res
Timothy Stephen Wallis
Edouard Eugenievich Galyov
Michael William Wood
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotech & Biolog Scien Res, Timothy Stephen Wallis, Edouard Eugenievich Galyov, Michael William Wood filed Critical Biotech & Biolog Scien Res
Priority to EP99937942A priority Critical patent/EP1058732A2/fr
Publication of WO1999045120A2 publication Critical patent/WO1999045120A2/fr
Publication of WO1999045120A3 publication Critical patent/WO1999045120A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/255—Salmonella (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

On prépare des micro-organismes atténués par mutation d'un ou de plusieurs gènes choisis parmi sopA, sopD, sipA, ou d'un gène tiré de l'îlot de pathogénicité SPI-5 lorsque le micro-organisme est Salmonella, ou d'un ou de plusieurs gènes homologues correspondant auxdits gènes de Salmonella, lorsque le micro-organisme est une autre entérobactérie. Par rapport aux souches de type sauvage, les micro-organismes présentent une entéropathogénicité réduite, mais sensiblement le même caractère envahissant. Ces micro-organismes atténués peuvent être administrés sous forme de vaccins contre les infections aux entérobactéries. L'invention concerne également les polypeptides codés par les gènes sopA, pipD, orfX, pipC, pipB et pipA, leurs variants, ainsi que les acides nucléiques codant ces polypeptides.
PCT/GB1999/000675 1998-03-06 1999-03-08 Attenuation de bacteries: matieres et methodes associees WO1999045120A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP99937942A EP1058732A2 (fr) 1998-03-06 1999-03-08 Attenuation de bacteries: matieres et methodes associees

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9804809.3 1998-03-06
GBGB9804809.3A GB9804809D0 (en) 1998-03-06 1998-03-06 Attenuated salmonella:materials and methods relating thereto

Publications (2)

Publication Number Publication Date
WO1999045120A2 WO1999045120A2 (fr) 1999-09-10
WO1999045120A3 true WO1999045120A3 (fr) 1999-12-16

Family

ID=10828124

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/000675 WO1999045120A2 (fr) 1998-03-06 1999-03-08 Attenuation de bacteries: matieres et methodes associees

Country Status (3)

Country Link
EP (1) EP1058732A2 (fr)
GB (1) GB9804809D0 (fr)
WO (1) WO1999045120A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2126332T3 (es) 1994-12-09 1999-03-16 Imp College Innovations Ltd Metodo para la identificacion de genes implicados en la adaptacion de un microorganismo a su entorno.
DE69942925D1 (de) 1998-09-04 2010-12-16 Emergent Product Dev Uk Ltd Abgeschwächte Salmonella SP12 Mutante als Antigen-Träger.
GB9910812D0 (en) 1999-05-10 1999-07-07 Microscience Ltd Vaccine composition
DE60107707T2 (de) * 2000-10-12 2005-12-08 Washington University St. Louis Mikroben mit einer transkriptionsabbrecher enthaltender dämpfender mutation
EP2326344A4 (fr) 2008-06-16 2013-08-07 Prokarium Ltd Vaccins vectorisés de salmonella contre chlamydia et procédés d utilisation
CA2933579A1 (fr) 2013-12-11 2015-06-18 University Of Massachusetts Compositions et procedes de traitement d'une maladie au moyen d'une proteine effectrice t3ss de salmonella (sipa)
CN106754594A (zh) * 2016-12-12 2017-05-31 扬州大学 一种猪霍乱沙门氏菌减毒载体菌及其构建方法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ABDELMOUNAAÏM ALLAOUI ET AL.: "Characterization of the Shigella flexneri ipgD and ipgF genes, which are located in the proximal part of the mxi locus", INFECTION AND IMMUNITY, vol. 61, no. 5, May 1993 (1993-05-01), WASHINGTON US, pages 1707 - 1714, XP002108939 *
CARMEN M. COLLAZO ET AL.: "The invasion-associated type-III protein secretion system in Salmonella - a review", GENE, vol. 192, no. 1, June 1997 (1997-06-01), AMSTERDAM NL, pages 51 - 59, XP004115688 *
EDOUARD E. GALYOV ET AL.: "A secreted effector protein of Salmonella dublin is translocated into eukaryotic cells and mediates inflammation and fluid secretion in infected ileal mucosa", MOLECULAR MICROBIOLOGY, vol. 25, no. 5, September 1997 (1997-09-01), pages 903 - 912, XP002108937 *
GUANGHUI WU ET AL.: "Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase", JOURNAL OF GENERAL MICROBIOLOGY, vol. 139, no. 8, August 1993 (1993-08-01), pages 1795 - 1805, XP002108936 *
HOWARD OCHMAN ET AL.: "Identification of a pathogenicity island required for Salmonella survival in host cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 93, no. 1, July 1996 (1996-07-01), WASHINGTON US, pages 7800 - 7804, XP002094896 *
JACEK OSTROWSKI ET AL.: "Characterization of the cysJIH regions of Salmonella typhimorium and Escherichia coli B", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 26, 15 September 1989 (1989-09-15), MD US, pages 15726 - 15737, XP002116160 *
JONES M A ET AL: "Secreted effector proteins of Salmonella dublin act in concert to induce enteritis.", INFECTION AND IMMUNITY, (1998 DEC) 66 (12) 5799-804. JOURNAL CODE: GO7. ISSN: 0019-9567., United States, XP002116161 *
MICHAEL W. WOOD ET AL.: "SopE, a secreted protein of Salmonella dublin, is translocated into the target eukaryotic cell via a sip-dependent mechanism and promotes bacterial entry", MOLECULAR MICROBIOLOGY, vol. 22, 1996, pages 327 - 338, XP002108938 *
NINA K. HEINZINGER ET AL.: "Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism", JOURNAL OF BACTERIOLOGY, vol. 177, no. 10, May 1995 (1995-05-01), pages 2813 - 2820, XP002108935 *

Also Published As

Publication number Publication date
WO1999045120A2 (fr) 1999-09-10
EP1058732A2 (fr) 2000-12-13
GB9804809D0 (en) 1998-04-29

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