WO1999037766A3 - A purified polynucleotide sequence containing a region encoding a mutated zap-70 protein or a fragment thereof - Google Patents

A purified polynucleotide sequence containing a region encoding a mutated zap-70 protein or a fragment thereof Download PDF

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Publication number
WO1999037766A3
WO1999037766A3 PCT/EP1999/000746 EP9900746W WO9937766A3 WO 1999037766 A3 WO1999037766 A3 WO 1999037766A3 EP 9900746 W EP9900746 W EP 9900746W WO 9937766 A3 WO9937766 A3 WO 9937766A3
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WO
WIPO (PCT)
Prior art keywords
protein
amino acid
nucleotide
zap
acid residue
Prior art date
Application number
PCT/EP1999/000746
Other languages
French (fr)
Other versions
WO1999037766A2 (en
Inventor
Oreste Acuto
Bartolo Vincenzo Di
Original Assignee
Pasteur Institut
Oreste Acuto
Bartolo Vincenzo Di
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut, Oreste Acuto, Bartolo Vincenzo Di filed Critical Pasteur Institut
Priority to AU28313/99A priority Critical patent/AU2831399A/en
Publication of WO1999037766A2 publication Critical patent/WO1999037766A2/en
Publication of WO1999037766A3 publication Critical patent/WO1999037766A3/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
    • G01N2333/91215—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases with a definite EC number (2.7.1.-)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to a purified polynucleotide containing a sequence encoding the linker region of the ZAP-70 protein, or a fragment thereof, wherein said sequence is mutated at least at one nucleotide located between nucleotide 1047 and nucleotide 1199 (corresponding respectively to amino acid residue 280 and amino acid residue 315). More particularly the mutation is between nucleotide 1155 and nucleotide 1184 (corresponding respectively to amino acid residue 316 and amino acid residue 325) and preferably at position 1064 (corresponding to amino acid residue 319). The present invention also provides a polypeptide corresponding to the polynucleotide according to the invention, and includes a method of screening for molecules capable of modifying the activation of T-cells wherein said molecules can interact positively or negatively on the binding of a ZAP-70 protein and a Src-Homology (SH) 2 domain-containing protein such as the p56lck signaling protein. The present invention also relates to active molecules as obtained according to the method of the invention and uses for the nucleic acids, polypeptides, and active molecules of the invention.
PCT/EP1999/000746 1998-01-26 1999-01-26 A purified polynucleotide sequence containing a region encoding a mutated zap-70 protein or a fragment thereof WO1999037766A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU28313/99A AU2831399A (en) 1998-01-26 1999-01-26 A purified polynucleotide sequence containing a region encoding a mutated zap-70protein or a fragment thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7245498P 1998-01-26 1998-01-26
US60/072,454 1998-01-26

Publications (2)

Publication Number Publication Date
WO1999037766A2 WO1999037766A2 (en) 1999-07-29
WO1999037766A3 true WO1999037766A3 (en) 1999-11-25

Family

ID=22107689

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000746 WO1999037766A2 (en) 1998-01-26 1999-01-26 A purified polynucleotide sequence containing a region encoding a mutated zap-70 protein or a fragment thereof

Country Status (2)

Country Link
AU (1) AU2831399A (en)
WO (1) WO1999037766A2 (en)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
LUPHER M L ET AL: "The Cbl phosphotyrosine-binding domain selects a D(N/D)XpY motif and binds to the Tyr292 negative regulatory phosphorylation site of ZAP-70", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 52, 26 December 1997 (1997-12-26), MD US, pages 33140 - 33144, XP002116575 *
MÈGE D ET AL: "Mutation of tyrosines 492/493 in the kinase domain of ZAP-70 affects multiple T-cell receptor signaling pathways", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 51, 20 December 1996 (1996-12-20), MD US, pages 32644 - 32652, XP002116576 *
REVESZ L ET AL: "Non-peptide itam mimics as ZAP-70 antagonists", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 7, no. 22, 18 November 1997 (1997-11-18), pages 2875-2878, XP004136548, ISSN: 0960-894X *
ROIFMAN C M: "A MUTATION IN ZAP-70 PROTEIN TYROSINE KINASE RESULTS IN A SELECTIVE IMMUNODEFICIENCY", JOURNAL OF CLINICAL IMMUNOLOGY, vol. 15, no. 6 (Supplement), 1 November 1995 (1995-11-01), pages 52S - 62S, XP000576860, ISSN: 0271-9142 *
WANGE R L ET AL: "F2(PMP)2-TAMZETA3, A NOVEL COMPETITIVE INHIBITOR OF THE BINDING OF ZAP-70 TO THE T CELL ANTIGEN RECEPTOR, BLOCKS EARLY T CELL SIGNALING", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 2, 13 January 1995 (1995-01-13), pages 944 - 948, XP002056490, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO1999037766A2 (en) 1999-07-29
AU2831399A (en) 1999-08-09

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