CA2215335A1 - Cauliflower floral meristem identity genes and methods of using same - Google Patents
Cauliflower floral meristem identity genes and methods of using sameInfo
- Publication number
- CA2215335A1 CA2215335A1 CA002215335A CA2215335A CA2215335A1 CA 2215335 A1 CA2215335 A1 CA 2215335A1 CA 002215335 A CA002215335 A CA 002215335A CA 2215335 A CA2215335 A CA 2215335A CA 2215335 A1 CA2215335 A1 CA 2215335A1
- Authority
- CA
- Canada
- Prior art keywords
- cal
- nucleic acid
- acid molecule
- provides
- molecule encoding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000003259 Brassica oleracea var. botrytis Species 0.000 title abstract 12
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 8
- 102000039446 nucleic acids Human genes 0.000 abstract 8
- 150000007523 nucleic acids Chemical class 0.000 abstract 8
- 101150041357 CAL gene Proteins 0.000 abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 108700028369 Alleles Proteins 0.000 abstract 2
- 235000011303 Brassica alboglabra Nutrition 0.000 abstract 2
- 240000007124 Brassica oleracea Species 0.000 abstract 2
- 235000011302 Brassica oleracea Nutrition 0.000 abstract 2
- 241000219194 Arabidopsis Species 0.000 abstract 1
- 101100165912 Arabidopsis thaliana CAL gene Proteins 0.000 abstract 1
- 235000011331 Brassica Nutrition 0.000 abstract 1
- 241000219198 Brassica Species 0.000 abstract 1
- 241000218922 Magnoliophyta Species 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Physiology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a nucleic acid molecule encoding a CAULIFLOWER
(CAL) gene product such as a nucleic acid molecule encoding Arabidopsis thatiana CAL and a nucleic acid molecule encoding Brassica oleracea CAL (BoCAL). The invention also provides a nucleic acid molecule encoding a truncated CAL gene product such as a nucleic acid molecule encoding Brassica oleracea var.
botrytis CAL (BobCAL). The invention also provides a nucleic acid containing the Arabidopsis thaliana CAL gene, a nucleic acid molecule containing the Brassica oleracea CAL gene and a nucleic acid molecule containing the Brassica oleracea var. botritis CAL gene. The invention further provides a kit for converting shoot meristem to floral meristem and a kit for promoting early flowering in an angiosperm.
The invention provides a CAL polypeptide and an antibody that specifically binds CAL polypeptides. In addition, the invention provides the truncated BobCAL polypeptide and an antibody that specifically binds truncated BobCAL polypeptide. The invention further provides a method of identifying a Brassica having a modified CAL CAL allele by detecting a polymorphism associated with a CAL CAL locus, where the CAL CAL locus comprises a modified CAL CAL allele that does not encode an active CAL gene product.
(CAL) gene product such as a nucleic acid molecule encoding Arabidopsis thatiana CAL and a nucleic acid molecule encoding Brassica oleracea CAL (BoCAL). The invention also provides a nucleic acid molecule encoding a truncated CAL gene product such as a nucleic acid molecule encoding Brassica oleracea var.
botrytis CAL (BobCAL). The invention also provides a nucleic acid containing the Arabidopsis thaliana CAL gene, a nucleic acid molecule containing the Brassica oleracea CAL gene and a nucleic acid molecule containing the Brassica oleracea var. botritis CAL gene. The invention further provides a kit for converting shoot meristem to floral meristem and a kit for promoting early flowering in an angiosperm.
The invention provides a CAL polypeptide and an antibody that specifically binds CAL polypeptides. In addition, the invention provides the truncated BobCAL polypeptide and an antibody that specifically binds truncated BobCAL polypeptide. The invention further provides a method of identifying a Brassica having a modified CAL CAL allele by detecting a polymorphism associated with a CAL CAL locus, where the CAL CAL locus comprises a modified CAL CAL allele that does not encode an active CAL gene product.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US1996/001041 WO1997027287A1 (en) | 1996-01-26 | 1996-01-26 | Cauliflower floral meristem identity genes and methods of using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2215335A1 true CA2215335A1 (en) | 1997-07-31 |
| CA2215335C CA2215335C (en) | 2009-01-06 |
Family
ID=22254655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002215335A Expired - Fee Related CA2215335C (en) | 1996-01-26 | 1996-01-26 | Cauliflower floral meristem identity genes and methods of using same |
Country Status (2)
| Country | Link |
|---|---|
| CA (1) | CA2215335C (en) |
| WO (1) | WO1997027287A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR9914592A (en) * | 1998-10-16 | 2001-10-23 | Univ California | Flowering suppression processes in transgenic plants |
| US6987214B1 (en) | 1998-10-16 | 2006-01-17 | The Regents Of The University Of California | Methods of suppressing flowering in transgenic plants |
| EP1055729A1 (en) * | 1999-05-18 | 2000-11-29 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Transgenic plants exhibiting an altered flowering time |
| WO2001014559A1 (en) * | 1999-08-19 | 2001-03-01 | Kumiai Chemical Industry Co., Ltd. | Gene regulating plant branching, vector containing the gene, microorganism transformed by the vector, and method for regulating plant branching by using the microorganism |
| BR0014750A (en) * | 1999-10-12 | 2002-07-02 | Mendel Biotechnology Inc | Transgenic vegetable, and, method to change the flowering time or the vernalization requirement of a vegetable |
-
1996
- 1996-01-26 WO PCT/US1996/001041 patent/WO1997027287A1/en unknown
- 1996-01-26 CA CA002215335A patent/CA2215335C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997027287A1 (en) | 1997-07-31 |
| CA2215335C (en) | 2009-01-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |