WO1999029159B1 - Plant senescence-associated genes - Google Patents

Plant senescence-associated genes

Info

Publication number
WO1999029159B1
WO1999029159B1 PCT/US1998/025799 US9825799W WO9929159B1 WO 1999029159 B1 WO1999029159 B1 WO 1999029159B1 US 9825799 W US9825799 W US 9825799W WO 9929159 B1 WO9929159 B1 WO 9929159B1
Authority
WO
WIPO (PCT)
Prior art keywords
gene
dna molecule
plant
seq
nucleotide sequence
Prior art date
Application number
PCT/US1998/025799
Other languages
French (fr)
Other versions
WO1999029159A9 (en
WO1999029159A1 (en
Inventor
Shimon Gepstein
Taleb Hajuoje
Amalia Rosner
Original Assignee
Vitality Biotechnologies Inc
Shimon Gepstein
Taleb Hajuoje
Amalia Rosner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vitality Biotechnologies Inc, Shimon Gepstein, Taleb Hajuoje, Amalia Rosner filed Critical Vitality Biotechnologies Inc
Priority to AU18047/99A priority Critical patent/AU1804799A/en
Priority to CA002318476A priority patent/CA2318476A1/en
Priority to EP98962909A priority patent/EP1045631A1/en
Publication of WO1999029159A1 publication Critical patent/WO1999029159A1/en
Publication of WO1999029159B1 publication Critical patent/WO1999029159B1/en
Publication of WO1999029159A9 publication Critical patent/WO1999029159A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8266—Abscission; Dehiscence; Senescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Isolated genes which are expressed during plant senescence are provided. In particular, a gene is provided which encodes a receptor-like protein kinase which is preferentially expressed in plant leaves early in the process of plant senescence. Additionally, a gene encoding S-adenosyl methionine synthase which is expressed during the process of plant senescence is provided. Promoters from genes expressed during plant senescence are provided and operably linked to a foreign gene to provide developmental-specific expression of the foreign gene in a transformed plant.

Claims

AMENDED CLAIMS[received by the International Bureau on 2 July 1999 (02.07.99); original claims 1-22 replaced by amended claims 1-21 (3 pages)]
1. An isolated DNA molecule comprising a senescence-associated receptor-like protein kinase (sark) gene.
2. The isolated DNA of claim 1, wherein said sark gene encodes a serine/threonine protein kinase.
3. An isolated DNA molecule comprising a nucleotide sequence selected from the group consisting of:
(a) SEQ ID NO: 1;
(b) a nucleotide sequence that has substantial sequence similarity with SEQ ID NO: 1; and
(c) a functional fragment of (a) or (b), wherein said DNA molecule encodes a senescence-associated regulatory protein kinase.
4. The DNA molecule of claim 3, wherein said DNA molecule comprises the nucleotide sequence of SEQ ID NO: 1.
5. An expression vector comprising said DNA molecule of claim 1 or 3.
6. A transformed host comprising said DNA molecule of claim 1.
7. A method of inhibiting plant senescence comprising the steps of:
(a) constructing an expression vector comprising a plant-compatible promoter;
(b) operably linking said promoter to a foreign gene selected from the group consisting of a sark antisense gene, a sam antisense gene, a ribozyme directed to a sark gene or a sam gene and an external guide sequence directed to a sark gene or a sam gene; and
(c) transforming a host.
55 -
8. An isolated DNA molecule comprising the nucleotide sequence of SEQ ID
9. An isolated peptide comprising the amino acid sequence of SEQ ID NO: 2.
10. An expression vector comprising said DNA of claim 8.
11. A transformed host comprising said DNA molecule of claim 8.
12. The isolated DNA molecule of claim 1, wherein said DNA molecule further comprises a region encoding a leucine rich domain.
13. The isolated DNA molecule of claim 1, wherein said gene is expressed in a plant prior to degradation of chloroplast protein LHC2.
14. The isolated DNA molecule of claim 13, wherein said gene is preferentially expressed in leaf tissue.
15. The isolated DNA molecule of claim 14, wherein expression of said gene is induced in a detached leaf tissue.
16. A method of controlling plant senescence, comprising the steps of:
(a) providing a genetic construct comprising a senescence-associated structural gene operably linked to a plant compatible promoter, said senescence- associated structural gene being selected from the group consisting of a sark/threonine protein kinase receptor structural gene, a structural gene comprising the nucleotide sequence of SEQ ID NO: 1, and a structural gene comprising the nucleotide sequence of SEQ ID NO: 6;
(b) transforming a plant with said genetic construct; and
(c) selecting and propagating a transformed plant.
- 56 -
17. The method of claim 16, wherein said sark/threonine protein kinase receptor structural gene is the nucleotide sequence of SEQ ID NO: 1.
18. The method of claim 17, wherein said plant compatible promoter is tissue- specific.
19. The method of claim 16, wherein said senescence-associated structural gene comprises said nucleotide sequence of SEQ ID NO: 6 and said plant-compatible promoter is a temporarily expressed promoter.
20. An isolated DNA molecule encoding a sam gene, wherein transcription of said sam gene increases in mature leaves of a plant.
21. The DNA molecule of claim 20, wherein the sequence of said DNA molecule comprises the nucleotide sequence of SEQ ID NO: 6.
PCT/US1998/025799 1997-12-08 1998-12-08 Plant senescence-associated genes WO1999029159A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU18047/99A AU1804799A (en) 1997-12-08 1998-12-08 Plant senescence-associated genes
CA002318476A CA2318476A1 (en) 1997-12-08 1998-12-08 Plant senescence-associated genes
EP98962909A EP1045631A1 (en) 1997-12-08 1998-12-08 Plant senescence-associated genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6789897P 1997-12-08 1997-12-08
US60/067,898 1997-12-08

Publications (3)

Publication Number Publication Date
WO1999029159A1 WO1999029159A1 (en) 1999-06-17
WO1999029159B1 true WO1999029159B1 (en) 1999-08-26
WO1999029159A9 WO1999029159A9 (en) 1999-09-30

Family

ID=22079142

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/025799 WO1999029159A1 (en) 1997-12-08 1998-12-08 Plant senescence-associated genes

Country Status (4)

Country Link
EP (1) EP1045631A1 (en)
AU (1) AU1804799A (en)
CA (1) CA2318476A1 (en)
WO (1) WO1999029159A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1130104A1 (en) * 2000-02-16 2001-09-05 Stichting Dienst Landbouwkundig Onderzoek Reduction of in planta degradation of recombinant plant products
KR100604195B1 (en) * 2004-09-02 2006-07-25 고려대학교 산학협력단 A novel senescence-specific plant gene and its promoter
PL2324703T3 (en) 2005-03-21 2015-11-30 Univ California Method for improving water use efficiency in plants

Also Published As

Publication number Publication date
AU1804799A (en) 1999-06-28
EP1045631A1 (en) 2000-10-25
WO1999029159A9 (en) 1999-09-30
CA2318476A1 (en) 1999-06-17
WO1999029159A1 (en) 1999-06-17

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