Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/001—Enzyme electrodes

Definitions

• the present invention relates to a method of determining the concentration of an analyte with the use of a bioelement according to the preamble to the main claim.
• the invention also relates to a device for determining the concentration of an analyte in accordance with the method of the invention.
• analyte Methods of determining the concentration of a compound, hereinafter referred to as an analyte, are of particular interest in the biomedical, industrial, environmental and agricultural/food fields. These methods provide for the use of biomolecules such as, for example, enzymes, antibodies, cells or the like, which can interact specifically with the analyte the concentration of which is to be determined.
• the interaction which may be chemical, chemico-physical or physical, is such as to bring about a change in the characteristics of the analyte-bioelement system which can be suitably converted into a signal, for example, an electrical signal, which can be correlated with the concentration of the analyte.
• transducers for detecting the above-mentioned changes and for converting them (with amperometric, potentiometric, conductometric, piezo-electric, optical, thermal transducers, etc.) into signals, normally electrical signals, which can be suitably amplified and processed, as well as displayed and/or recorded.
• the concentration of the analyte is generally determined on the basis of a measurement of the rate of analyte-bioelement interaction, that is, of the rate of transfer of the analyte to the bioelement.
• rates depend upon factors such as the activity of the bioelement, the temperature, the viscosity and the pH, as well as on the nature and physical characteristics of the matrix immobilizing the bioelement.
• This necessitates the production of a calibration curve correlating the signals generated by the transducer with the concentration of the analyte for each measurement or set of measurements of the analyte concentration.
• the problem upon which the present invention is based is that of providing a method and a device for determining the concentration of an analyte with the use of a bioelement which overcomes all of the problems complained of with reference to the prior art mentioned.
• Figure 1 is a schematic plan view of a device formed in accordance with the invention
• Figure 2 is a section taken on the line II-II of Figure 1.
• a device for determining the concentration of an analyte with the use of a bioelement according to the present invention is generally indicated 1.
• the device 1 comprises a chamber 2 for holding the sample in which the analyte whose concentration is to be determined is present.
• the chamber 2 is defined by a first surface and a second surface disposed opposite one another and indicated 3 and 4 respectively, and by a further lateral surface 5.
• Holes formed in the surface 4 put the chamber 2 into communication with a first, inlet capillary duct 6 and a second, outlet capillary duct 7 for admitting the sample containing the analyte to be analyzed to the chamber 2 and discharging it therefrom, respectively.
• the chamber 2 is formed by superimposing two plate-like elements defining the surfaces 3 and 4 with the interposition of a film seal 8 of which the thickness, indicated H in Figure 2, defines the distance between the aforementioned surfaces 3 and 4.
• a hole in the seal 8 defines the lateral surface 5 of the chamber and has a shape which is elongate in plan towards the inlet and outlet openings of the chamber, as shown in Figure 1, in which D indicates the length of the chamber in the direction in which it is elongate.
• the depth H of the chamber 2, which is equal to the distance between the surfaces 3 and 4, is selected so as to be equal to or less than 500 micrometres and, advantageously, between 2 and 200 micrometres.
• a preferred selection for the depth H is between 20 and 100 micrometres.
• the selected ratio between the length D and the depth H (the distance between the surfaces 3 and 4) of the chamber 2 is selected so as to be between 10 and 2000 and is preferably between 50 and 500.
• the surface 4 is preferably defined by the active surface of a transducer 9 shown only schematically in Figure 1, which can detect the chemical, chemico-physical , or physical changes due to the interaction of the analyte with a preselected bioelement introduced into the chamber 2, and can convert these changes into a measurable physical magnitude correlated with the concentration of the analyte to be determined, as will be explained further in the following description.
• the transducer may be electrochemical (amperometric, potentiometric, conductometric) , optical, piezo-electric, thermal, etc., according to the physical magnitude to be measured.
• the surface 4 may also be transparent to the signal used for measuring the bioelement-analyte interaction, to permit detection by an external transducer.
• the bioelement selected for interacting with the analyte is arranged on the surface 3 opposite the transducer 9.
• the bioelement may be immobilized on the surface 3 chemically and/or physically.
• the bioelement may be introduced into the chamber 2 directly with the sample containing the analyte to be analyzed.
• the bioelement may be immobilized on the same surface as the transducer or the two surfaces may form portions of the transducer.
• the plate-like element defining the surface 3 on which the bioelement can be immobilized is associated with the device 1 interchangeably and may, for example, be in the form of a glass plate.
• the concentration of the analyte present in the sample is determined the following manner.
• the sample containing the analyte to be analyzed is introduced into the chamber 2 through the inlet duct 6 by capillarity or simply by injection, possibly with the addition of a buffer, a mediator, and/or other substance capable of facilitating the interaction with the transducer 9.
• the interaction of the analyte with the bioelement which may be disposed on the surface 3 or introduced into the chamber 2 directly with the sample, brings about a change in the chemical, chemico- physical and/or physical characteristics of the analyte- bioelement system.
• At least one of these characteristics changes over time from the moment when the analyte- bioelement interaction starts up to a final moment at which all of the analyte has interacted with the bioelement.
• the characteristics of the analyte-bioelement system are detected and converted by the transducer 9 into a signal variable within the period of time between the aforementioned starting and final moments in dependence on the activity of the bioelement and tending towards a constant value or towards zero after the analyte-bioelement interaction has been completed.
• the signal detected in the absence of the bioelement is subtracted from the signal detected during a measurement carried out in the presence of the bioelement so as to isolate solely the signal generated as a result of the analyte-bioelement interaction.
• the large ratio between the length D and the depth H of the chamber which is representative of the surface area: volume ratio of the chamber, together with the small depth H thereof, enables the analyte to diffuse rapidly between the surfaces 3 and 4 of the chamber 2 within periods of the order of one minute or less.
• the measurement is carried out in conditions in which the solution to be analyzed is substantially still, that is, making use solely of the motion of the molecular diffusion of the analyte-bioelement system and not in conditions providing for a flow and/or stirring of the aforesaid solution.
• a high D:H ratio and a small depth H also achieve a high concentration of the bioelement within the chamber 2 and consequently a high activity of the bioelement per unit volume, even with a small number of molecules of the bioelement (for example, it suffices to provide a monomolecular layer of the selected bioelement on the surface 3) .
• the quantity of bioelement in the chamber 2 and the diffusion times between the surfaces 3, 4 defining the chamber are in any case such as to ensure that all of the analyte interacts with the bioelement within periods of the order of a few minutes.
• the signal detected by the transducer tends towards a value which is substantially constant within the experimental error.
• the magnitude measured, or its variation, is proportional to the initial concentration of the analyte, as will become clearer from the following description of the examples . The measurement of this magnitude is therefore independent of the activity of the bioelement.
• the concentration of the analyte is determined according to the nature of the analyte, the nature of the bioelement, and the type of analyte-bioelement interaction.
• the surface 4 is constituted by a thin sheet of a noble metal (Au, Pt, etc.), the electrical potential of which, measured relative to a reference electrode, for example, in Ag-AgCl, varies in dependence on the [ferricyanide] : [ferrocyanide] molar ratio, where the ferrocyanide represents the product of the reaction of the sulphite-sulphite oxidase-ferricyanide system, since the sulphite oxidase (the bioelement) is immobilized on the surface 3 and the ferricyanide (mediator) is in solution.
• a glass electrode may be used instead of the noble metal plate and the change in pH which accompanies the aforementioned reaction may be measured. In both cases, the trans
• the transducer used comprises a piezo-electric crystal on the surface 4 of which the aforesaid antibodies are immobilized.
• the concentration of the analyte is determined by measurement of the change in the resonance frequency of the piezo-electric crystal due to the change in the mass of the piezo-electric crystal -antibody system as a result of the binding of all of the antigen to the immobilized antibody.
• the transducer selected is a piezo-electric transducer .
• the surface 4 is constituted by that of a phototube (transducer) or that of a medium which is optically transparent to the photons emitted, or in any case such as to allow the photons to interact with the transducer.
• the concentration of the analyte is determined by measurement of the number of photons emitted during the interaction of all of the ATP with the luciferase (bioelement) in the presence of an excess of luciferin, the luciferase being immobilized on the surface 3.
• the transducer used is an optical transducer.
• Example 1 Determination of the concentration of glucose with the use of glucose oxidase and peroxidase enzymes and with the use of an amperometric transducer.
• the bioelements (glucose oxidase and peroxidase) were immobilized covalently on the surface of a suitably prepared glass plate which defined one surface of the chamber.
• the quantities of glucose oxidase and peroxidase immobilized were such as to ensure that all of the glucose present in the chamber was converted within periods of less than one minute.
• the opposite surface was defined by a transducer comprising a vitreous graphite electrode constituting the working electrode of a voltammetric circuit which further comprised a reference electrode and a contro-electrode.
• the depth H of the chamber was 100 micrometres with a chamber volume of 2 microlitres.
• the sample to be analyzed which was constituted by a glucose solution, was diluted with a concentrated pH 6.5 sodium phosphate solution containing ImM (millimolar) hydroquinone , to give a 0. ImM glucose solution.
• a potential of -300 mV relative to the reference electrode in Ag/AgCl was applied to the working electrode and the electrical current flowing in the electrochemical circuit was measured until the value of the current due to the reduction of the quinone formed during the oxidation of the glucose via glucose oxidase-peroxidase, fell substantially to zero, that is, until all of the quinone which was formed in an equivalent quantity to the glucose had been reduced.
• the electrical -current signal generated was integrated over time and the quantity of electrical charge thus measured was 39 microcoulombs , which value corresponds, within the experimental error, to the value calculated theoretically on the basis of the glucose present in the chamber.
• the opposed surfaces of the chamber were defined by two flat electrodes of a conductometric circuit .
• the solution to be analyzed contained a ImM concentration of urea (analyte) .
• a small quantity of a solution containing the urease enzyme (this solution was equal in quantity to one hundredth of the volume of the solution of analyte and the final concentration of the enzyme in the chamber was such as to enable all of the urea to be converted within a period of less than one minute) was added to the solution containing the analyte and the increase in the conductivity of the solution due to the conversion of all of the urea into ammonium ions and bicarbonate ions was measured.
• the increase in conductivity measured which was 5.2 mSiemens, corresponded approximately, within the experimental error, to the value calculated theoretically on the basis of the initial concentration of urea in the chamber.
• the invention thus solves the problem set, achieving many advantages in comparison with known solutions .
• the signal generated by the transducer which is proportional to the concentration of the analyte, is measured after all of the analyte has interacted with the bioelement, even very marked variations in the activity of the bioelement do not affect the change in the signal detected and it is therefore not necessary to calibrate the instrumentation for each measurement or set of measurements to be made .
• Another advantage is that, owing to the extremely small volume of the chamber of the device of the invention, the quantity of bioelement necessary to determine the concentration of the analyte is greatly reduced, consequently reducing analysis costs.
• a further advantage connected with the small volumes of the chamber of the device of the invention is that, with the same plate on which the bioelement is disposed, it is possible to perform several analyte determinations relative to the bioelement immobilized.
• the interchangeability of the plate on which the bioelement is disposed enables the concentration of different analytes to be measured according to the type of bioelement selected.

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• Chemical & Material Sciences (AREA)
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Citations (6)

• 1997
  • 1997-08-08 IT ITPD970183 patent/IT1294642B1/it active IP Right Grant
• 1998
  • 1998-07-31 DE DE69832090T patent/DE69832090T2/de not_active Expired - Lifetime
  • 1998-07-31 WO PCT/IT1998/000224 patent/WO1999008106A1/it active IP Right Grant
  • 1998-07-31 EP EP98937775A patent/EP1027602B1/en not_active Expired - Lifetime
  • 1998-07-31 AU AU86463/98A patent/AU8646398A/en not_active Abandoned
  • 1998-07-31 ES ES98937775T patent/ES2251776T3/es not_active Expired - Lifetime

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