WO1999006570A3 - Plasmids - Google Patents

Plasmids Download PDF

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Publication number
WO1999006570A3
WO1999006570A3 PCT/EP1998/005007 EP9805007W WO9906570A3 WO 1999006570 A3 WO1999006570 A3 WO 1999006570A3 EP 9805007 W EP9805007 W EP 9805007W WO 9906570 A3 WO9906570 A3 WO 9906570A3
Authority
WO
WIPO (PCT)
Prior art keywords
plasmids
propagation
absence
disclosed
selectable marker
Prior art date
Application number
PCT/EP1998/005007
Other languages
French (fr)
Other versions
WO1999006570A2 (en
Inventor
Andrew Peter Goldsbrough
Original Assignee
Unilever Plc
Unilever Nv
Andrew Peter Goldsbrough
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Plc, Unilever Nv, Andrew Peter Goldsbrough filed Critical Unilever Plc
Priority to AU91619/98A priority Critical patent/AU9161998A/en
Publication of WO1999006570A2 publication Critical patent/WO1999006570A2/en
Publication of WO1999006570A3 publication Critical patent/WO1999006570A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)

Abstract

Recombinant plasmids comprising a non-selectable marker, their propagation in the absence of selection pressure and their use in genetic modification techniques are disclosed.
PCT/EP1998/005007 1997-07-29 1998-07-27 Plasmids WO1999006570A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU91619/98A AU9161998A (en) 1997-07-29 1998-07-27 Plasmids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97305694.8 1997-07-29
EP97305694 1997-07-29

Publications (2)

Publication Number Publication Date
WO1999006570A2 WO1999006570A2 (en) 1999-02-11
WO1999006570A3 true WO1999006570A3 (en) 1999-04-22

Family

ID=8229444

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/005007 WO1999006570A2 (en) 1997-07-29 1998-07-27 Plasmids

Country Status (2)

Country Link
AU (1) AU9161998A (en)
WO (1) WO1999006570A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0154430A1 (en) * 1984-02-17 1985-09-11 Eli Lilly And Company Novel cloning vectors containing selectable genetic markers for use in streptomyces and related organisms
US4753876A (en) * 1984-03-21 1988-06-28 Monsanto Company Marker genes in pseudomonad bacteria
US5102797A (en) * 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
WO1992011376A1 (en) * 1990-12-21 1992-07-09 Amylogene Hb Genetically engineered modification of potato to form amylopectin-type starch
WO1995007463A1 (en) * 1993-09-10 1995-03-16 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
WO1997010347A1 (en) * 1995-09-15 1997-03-20 Howard John A Expression cassettes and methods for delivery of animal vaccines

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0154430A1 (en) * 1984-02-17 1985-09-11 Eli Lilly And Company Novel cloning vectors containing selectable genetic markers for use in streptomyces and related organisms
US4753876A (en) * 1984-03-21 1988-06-28 Monsanto Company Marker genes in pseudomonad bacteria
US5102797A (en) * 1989-05-26 1992-04-07 Dna Plant Technology Corporation Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system
WO1992011376A1 (en) * 1990-12-21 1992-07-09 Amylogene Hb Genetically engineered modification of potato to form amylopectin-type starch
WO1995007463A1 (en) * 1993-09-10 1995-03-16 The Trustees Of Columbia University In The City Of New York Uses of green fluorescent protein
WO1997010347A1 (en) * 1995-09-15 1997-03-20 Howard John A Expression cassettes and methods for delivery of animal vaccines

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"BACULOVIRUS TRANSFER VECTORS", INVITROGEN CATALOGUE, XX, XX, 1 January 1992 (1992-01-01), XX, pages A + B, XP002092498 *
CHAKRABARTI S. AND MOSS B.: "New Vaccinia Virus expression vector", VACCINES 86, NEW APPROACHES IMMUN., 1986, pages 289 - 292, XP002049925 *
LORENZO DE V.: "Designing microbial systems for gene expression in the field", TRENDS IN BIOTECHNOLOGY, vol. 12, September 1994 (1994-09-01), pages 365 - 371, XP002037464 *
SILHAVY T.J. AND BECKWITH J.R.: "Uses of lac fusions for the study of biologicals problems", MICROBIOLOGICAL REVIEWS, vol. 49, no. 4, 1 December 1985 (1985-12-01), pages 398 - 418, XP000570644 *

Also Published As

Publication number Publication date
WO1999006570A2 (en) 1999-02-11
AU9161998A (en) 1999-02-22

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