WO1999003984A3 - Proteases pour organismes gram-positifs - Google Patents

Proteases pour organismes gram-positifs Download PDF

Info

Publication number
WO1999003984A3
WO1999003984A3 PCT/US1998/014647 US9814647W WO9903984A3 WO 1999003984 A3 WO1999003984 A3 WO 1999003984A3 US 9814647 W US9814647 W US 9814647W WO 9903984 A3 WO9903984 A3 WO 9903984A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
gram
provides
proteases
nucleic acid
Prior art date
Application number
PCT/US1998/014647
Other languages
English (en)
Other versions
WO1999003984A2 (fr
Inventor
David A Estell
Original Assignee
Genencor Int
Genencor Int Bv
David A Estell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US09/462,845 priority Critical patent/US6723550B1/en
Priority to JP2000503190A priority patent/JP2001510037A/ja
Priority to EP98935681A priority patent/EP0991754A2/fr
Priority to AU84870/98A priority patent/AU8487098A/en
Application filed by Genencor Int, Genencor Int Bv, David A Estell filed Critical Genencor Int
Publication of WO1999003984A2 publication Critical patent/WO1999003984A2/fr
Publication of WO1999003984A3 publication Critical patent/WO1999003984A3/fr
Priority to US10/401,436 priority patent/US6911333B2/en
Priority to US10/402,067 priority patent/US6881562B2/en
Priority to US10/401,437 priority patent/US6849440B2/en
Priority to US11/014,051 priority patent/US7329525B2/en
Priority to US11/014,339 priority patent/US7329526B2/en
Priority to US11/013,991 priority patent/US20050101001A1/en
Priority to US11/014,364 priority patent/US7316920B2/en
Priority to US11/014,432 priority patent/US7329527B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

L'invention concerne l'identification de nouvelles sérine protéases dans des micro-organismes Gram-positifs. Elle concerne les séquences d'acides nucléiques et d'acides aminés pour les sérine protéases de Bacillus subtilis SP1, SP2, SP3, SP4 et SP5. Elle concerne également des cellules hôtes présentant une mutation ou une délétion de la totalité ou d'une partie du gène codant SP1, SP2, SP3, SP4 et SP5. Elle concerne également des cellules hôtes comprenant un acide nucléique codant des protéines hétérologues souhaitées, telles que des enzymes. Elle concerne enfin une composition de nettoyage contenant cette sérine protéase.
PCT/US1998/014647 1997-07-15 1998-07-14 Proteases pour organismes gram-positifs WO1999003984A2 (fr)

Priority Applications (12)

Application Number Priority Date Filing Date Title
US09/462,845 US6723550B1 (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms
JP2000503190A JP2001510037A (ja) 1997-07-15 1998-07-14 グラム陽性微生物からのプロテアーゼ
EP98935681A EP0991754A2 (fr) 1997-07-15 1998-07-14 Proteases pour organismes gram-positifs
AU84870/98A AU8487098A (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms
US10/401,437 US6849440B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/401,436 US6911333B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/402,067 US6881562B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US11/014,051 US7329525B2 (en) 1997-07-15 2004-12-16 Serine proteases from gram-positive microorganisms
US11/014,339 US7329526B2 (en) 1997-07-15 2004-12-16 Serine proteases from-gram-positive microorganisms
US11/013,991 US20050101001A1 (en) 1997-07-15 2004-12-16 Proteases from gram-position organisms
US11/014,364 US7316920B2 (en) 1997-07-15 2004-12-16 Serine proteases from gram-positive microorganisms
US11/014,432 US7329527B2 (en) 1997-07-15 2004-12-16 Serine proteases from gram-positive microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97305232 1997-07-15
EP97305232.7 1997-07-15

Related Child Applications (6)

Application Number Title Priority Date Filing Date
US09462845 A-371-Of-International 1998-07-14
US09/462,845 A-371-Of-International US6723550B1 (en) 1997-07-15 1998-07-14 Proteases from gram-positive organisms
US10/401,437 Division US6849440B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/401,436 Division US6911333B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/402,067 Division US6881562B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms
US10/402,312 Division US6833261B2 (en) 1997-07-15 2003-03-26 Proteases from gram-positive organisms

Publications (2)

Publication Number Publication Date
WO1999003984A2 WO1999003984A2 (fr) 1999-01-28
WO1999003984A3 true WO1999003984A3 (fr) 1999-04-08

Family

ID=8229415

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/014647 WO1999003984A2 (fr) 1997-07-15 1998-07-14 Proteases pour organismes gram-positifs

Country Status (4)

Country Link
EP (1) EP0991754A2 (fr)
JP (1) JP2001510037A (fr)
AU (1) AU8487098A (fr)
WO (1) WO1999003984A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9724629D0 (en) * 1997-11-20 1998-01-21 Genencor Int Bv Alpha/beta hydrolase-fold enzymes
AU2003228393A1 (en) 2002-03-29 2003-10-13 Genencor International, Inc. Ehanced protein expression in bacillus
US8518675B2 (en) 2005-12-13 2013-08-27 E. I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7723083B2 (en) 2005-12-13 2010-05-25 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US8288136B2 (en) 2005-12-13 2012-10-16 E. I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7964378B2 (en) 2005-12-13 2011-06-21 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
US7951566B2 (en) 2005-12-13 2011-05-31 E.I. Du Pont De Nemours And Company Production of peracids using an enzyme having perhydrolysis activity
CN102177234A (zh) 2008-08-13 2011-09-07 纳幕尔杜邦公司 酶促过酸制备的控制
WO2010039960A1 (fr) 2008-10-03 2010-04-08 E.I. Dupont De Nemours And Company Stabilisation de perhydrolases dans une formulation comprenant un ester d'acide carboxylique
EP2458985B1 (fr) 2009-07-27 2014-11-05 E. I. du Pont de Nemours and Company Préparation enzymatique <i>in situ</i> de compositions de revêtement antimicrobiennes, aptes à être retirées, à base de peracide, et procédés d'utilisation
US8222012B2 (en) 2009-10-01 2012-07-17 E. I. Du Pont De Nemours And Company Perhydrolase for enzymatic peracid production
CN102952787B (zh) * 2012-10-22 2014-07-16 南京工业大学 一种乙酰木聚糖酯酶及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0369817A2 (fr) * 1988-11-18 1990-05-23 Omnigene Bioproducts, Inc. Souches de bacillus

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
EMBL/GENBANK/DDBJ DATABASES Accession no O32120, Sequence reference O32120 1 January 1998 *
EMBL/GENBANK/DDBJ DATABASES Accession no P70948, Sequence reference P70948 1 February 1997 LEVINE A ET AL: "YITV" *
KUNST F. ET AL: "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis", NATURE, vol. 390, 20 November 1997 (1997-11-20), pages 249 - 256, XP002080813 *
LEVINE A ET AL: "A 10.3 kbp segment from nprB to argJ at the 102 region of the Bacillus subtilis chromosome", MICROBIOLOGY, vol. 143, January 1997 (1997-01-01), pages 175 - 177, XP002080812 *

Also Published As

Publication number Publication date
JP2001510037A (ja) 2001-07-31
EP0991754A2 (fr) 2000-04-12
AU8487098A (en) 1999-02-10
WO1999003984A2 (fr) 1999-01-28

Similar Documents

Publication Publication Date Title
WO1999014341A3 (fr) Proteases extraites d&#39;organismes gram positif
WO1999033960A3 (fr) Proteases de germes gram positifs
WO1999034003A3 (fr) Proteases provenant d&#39;organismes a gram positif
EP0583339B1 (fr) Enzymes stabilisees et compositions detergentes
EP0516200B1 (fr) Compositions de détergents contenant des enzymes stabilisés
EP2399992A2 (fr) Variantes de subtilase
WO1999003984A3 (fr) Proteases pour organismes gram-positifs
Wetmore et al. The role of the pro‐sequence in the processing and secretion of the thermolysin‐like neutral protease from Bacillus cereus
WO2005052161A3 (fr) Serine proteases, acides nucleiques codant des enzymes de serine et vecteurs et cellules hotes les integrant
AU2003222165A1 (en) Methods for producing secreted polypeptides having l-asparaginase activity
NO964997L (no) DNA som koder for Bacillus licheniformis PWD-1 keratinase
US7329525B2 (en) Serine proteases from gram-positive microorganisms
EP0761815A3 (fr) Gène de sporulation de Bacillus thuringiensis
CA2253006A1 (fr) Gene hydroxylase de beta-carotene
WO1999004016A3 (fr) Proteases pour germes gram positif
US10894954B2 (en) Subtilisin variants and uses thereof
WO1999034001A3 (fr) Proteases provenant d&#39;organismes a gram positif
WO1999034002A3 (fr) Proteases provenant d&#39;organismes a gram positif
WO1999004007A8 (fr) Augmentation de la production de proteines dans les micro-organismes gram-positifs
WO1999033959A3 (fr) Proteases provenant d&#39;organismes a gram positif
AU8405698A (en) Increasing production of proteins in gram-positive microorganisms
Tanaka et al. Stability of thermostable enzyme, aqualysin I; a subtilisin-type serine protease from Thermus aquaticus YT-1
Strongin et al. Intracellular serine proteases of bacilli
ATE380874T1 (de) Alpha-1,4-glucan lyase aus einem pilz, ihre reinigung, genklonierung und expression in mikroorganismen
Estell Proteases from gram positive organisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG US UZ VN

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 09462845

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 1998935681

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1998935681

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: CA

WWR Wipo information: refused in national office

Ref document number: 1998935681

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1998935681

Country of ref document: EP