WO1998059314B1 - Bacteria colony counter and classifier - Google Patents

Bacteria colony counter and classifier

Info

Publication number
WO1998059314B1
WO1998059314B1 PCT/US1998/013034 US9813034W WO9859314B1 WO 1998059314 B1 WO1998059314 B1 WO 1998059314B1 US 9813034 W US9813034 W US 9813034W WO 9859314 B1 WO9859314 B1 WO 9859314B1
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
colonies
processor
dish
tray
Prior art date
Application number
PCT/US1998/013034
Other languages
French (fr)
Other versions
WO1998059314A1 (en
Filing date
Publication date
Priority claimed from US09/083,723 external-priority patent/US6002789A/en
Application filed filed Critical
Priority to AU81620/98A priority Critical patent/AU8162098A/en
Publication of WO1998059314A1 publication Critical patent/WO1998059314A1/en
Publication of WO1998059314B1 publication Critical patent/WO1998059314B1/en

Links

Abstract

The present invention relates to a colony counter and classifier for bacterial specimens on a petri dish. The device includes a housing having a tray which accepts the petri dish containing the bacteria colonies. A line scan camera is mounted within the housing above the tray. Upon actuation, a linear motor transports the tray across the scanning line of vision for the line scan camera. Simultaneously, a fiber optic illumination system illuminates the petri dish from its side opposite the camera. As the tray is transported by the linear motor, the line scan camera successively obtains an optical image of the petri dish containing the bacteria colonies. Each line scan has a width equal to one pixel and the resulting optical images from the line scan camera are combined together to form an overall optical image of high resolution. That image is stored utilizing a digital computer. The computer then analyzes the stored optical image to both identify and count the bacteria colonies. Advanced software is also used to differentiate between single colonies and two or more colonies which abut against each other.

Claims

AMENDED CLAIMS[received by the International Bureau on 21 December 1998 (21.12.98); original claims 1 and 3-7 amended; remaining claims unchanged (2 pages)]
1. A system for automatically counting bacteria colonies in a dish containing a translucent bacteria supporting media comprising:
a line scanning camera having an output signal representative of light intensity along a predetermined scan line, said scan line having a predetermined thickness, a conveyor which moves the dish along a predetermined path of movement substantially perpendicular to and intersecting said scan line, a light source optically coupled with the dish during movement along said path of movement, a processor having digital memory, said processor inputting said camera output signal into memory in synchronism with the movement of dish corresponding to said scan line thickness to thereby store a digitized video image of the dish in said memory, said processor being programmed to differentiate bacteria colonies from the bacteria supporting media and count said bacteria colonies from the digitized video image stored in memory.
2. The invention as defined in claim 1 wherein said scan line thickness corresponds to one pixel.
3. The invention as defined in claim 1 wherein said light source is optically coupled by a fiber optic bundle having one end exposed to said light source and a second end facing the dish on the side opposite from the line scan camera.
4. The invention as defined in claim 1 wherein said processor comprises a microprocessor.
5. The invention as defined in claim 1 wherein said conveyor comprises a slidable tray and a linear motor for moving said tray.
6. The invention as defined in claim 1 wherein said processor is programmed to differentiate between abutting bacteria colonies which form a bacteria cluster.
7. The invention as defined in claim 6 wherein said processor differentiates between abutting bacteria colonies by mathematically inserting a cone into the bacteria cluster until an outer edge portion of the cone corresponds to an outer edge portion of the bacteria cluster, and then masking the area within the cone.
PCT/US1998/013034 1997-06-24 1998-06-22 Bacteria colony counter and classifier WO1998059314A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU81620/98A AU8162098A (en) 1997-06-24 1998-06-22 Bacteria colony counter and classifier

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US5060597P 1997-06-24 1997-06-24
US60/050,605 1997-06-24
US09/083,723 US6002789A (en) 1997-06-24 1998-05-22 Bacteria colony counter and classifier
US09/083,723 1998-05-22

Publications (2)

Publication Number Publication Date
WO1998059314A1 WO1998059314A1 (en) 1998-12-30
WO1998059314B1 true WO1998059314B1 (en) 1999-02-18

Family

ID=26728450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/013034 WO1998059314A1 (en) 1997-06-24 1998-06-22 Bacteria colony counter and classifier

Country Status (3)

Country Link
US (1) US6002789A (en)
AU (1) AU8162098A (en)
WO (1) WO1998059314A1 (en)

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