WO1998058293A3 - Method and device for immobilizing macromolecules - Google Patents

Method and device for immobilizing macromolecules Download PDF

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Publication number
WO1998058293A3
WO1998058293A3 PCT/EP1998/003657 EP9803657W WO9858293A3 WO 1998058293 A3 WO1998058293 A3 WO 1998058293A3 EP 9803657 W EP9803657 W EP 9803657W WO 9858293 A3 WO9858293 A3 WO 9858293A3
Authority
WO
WIPO (PCT)
Prior art keywords
macromolecules
solid phase
immobilizing
light
bio
Prior art date
Application number
PCT/EP1998/003657
Other languages
German (de)
French (fr)
Other versions
WO1998058293A2 (en
Inventor
Holger Breter
Winfried Jentsch
Original Assignee
Biotools Inst Fuer Computerint
Holger Breter
Winfried Jentsch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotools Inst Fuer Computerint, Holger Breter, Winfried Jentsch filed Critical Biotools Inst Fuer Computerint
Priority to AU85387/98A priority Critical patent/AU8538798A/en
Publication of WO1998058293A2 publication Critical patent/WO1998058293A2/en
Publication of WO1998058293A3 publication Critical patent/WO1998058293A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/14—Peptides being immobilised on, or in, an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00373—Hollow needles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00427—Means for dispensing and evacuation of reagents using masks
    • B01J2219/0043—Means for dispensing and evacuation of reagents using masks for direct application of reagents, e.g. through openings in a shutter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00711—Light-directed synthesis
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Nanotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Biomedical Technology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Composite Materials (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The invention relates to a method and a device for immobilizing macromolecules according to the generic part of claims 1 and 19, 20, 21 and 22. The aim of the invention is to enable light induced, addressable immobilization of bio-macromolecules on extremely small surface elements. This is done by immobilizing macromolecules on a solid phase involving photoactivation of corresponding functional groups of the solid phase and coupling of reaction-capable groups of bio-macromolecules contained in a solution and the solid phase. The invention is characterized in that the immobilization reaction is photoactivated by miniaturizing the effect of the light on the surface of the solid phase, whereby addressable control of the effect of the light on the surface occurs according to a given model.
PCT/EP1998/003657 1997-06-18 1998-06-18 Method and device for immobilizing macromolecules WO1998058293A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85387/98A AU8538798A (en) 1997-06-18 1998-06-18 Method and device for immobilizing macromolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19726634.7 1997-06-18
DE19726634A DE19726634A1 (en) 1997-06-18 1997-06-18 Method and device for immobilizing macromolecules

Publications (2)

Publication Number Publication Date
WO1998058293A2 WO1998058293A2 (en) 1998-12-23
WO1998058293A3 true WO1998058293A3 (en) 1999-04-15

Family

ID=7833394

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/003657 WO1998058293A2 (en) 1997-06-18 1998-06-18 Method and device for immobilizing macromolecules

Country Status (3)

Country Link
AU (1) AU8538798A (en)
DE (1) DE19726634A1 (en)
WO (1) WO1998058293A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE296677T1 (en) 1998-08-28 2005-06-15 Febit Ag CARRIER FOR ANALYTE DETERMINATION METHODS AND METHOD FOR PRODUCING THE SUPPORT
DE10051396A1 (en) 2000-10-17 2002-04-18 Febit Ferrarius Biotech Gmbh An integrated synthesis and identification of an analyte, comprises particles immobilized at a carrier to be coupled to receptors in a structured pattern to give receptor arrays for biochemical reactions
US6835534B2 (en) * 2000-10-27 2004-12-28 The Penn State Research Foundation Chemical functionalization nanolithography
SE0103021D0 (en) * 2001-09-12 2001-09-12 Sven Oscarsson Immobilization method and surfaces produced using said method
GB0125133D0 (en) * 2001-10-19 2001-12-12 Univ Manchester Methods of patterning a monlayer

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0175973A1 (en) * 1984-09-28 1986-04-02 ORPEGEN Medizinisch-Molekularbiologische Forschungsgesellschaft m.b.H Support material for the use in immunoassays
EP0419369A1 (en) * 1989-09-22 1991-03-27 Sim (Societe D'investissement Dans La Microscopie) Sa Optical near-field method for microlithography and microlithography devices using this method
WO1996033971A1 (en) * 1995-04-25 1996-10-31 The Government Of The United States Of America, Represented By The Secretary Of The Navy Photoactivatable polymers for producing patterned biomolecular assemblies

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0175973A1 (en) * 1984-09-28 1986-04-02 ORPEGEN Medizinisch-Molekularbiologische Forschungsgesellschaft m.b.H Support material for the use in immunoassays
EP0419369A1 (en) * 1989-09-22 1991-03-27 Sim (Societe D'investissement Dans La Microscopie) Sa Optical near-field method for microlithography and microlithography devices using this method
WO1996033971A1 (en) * 1995-04-25 1996-10-31 The Government Of The United States Of America, Represented By The Secretary Of The Navy Photoactivatable polymers for producing patterned biomolecular assemblies

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"UTILIZING OPTICAL LITHOGRAPHY IN THE SUB-MICRON DIMENSIONAL REGIME", IBM TECHNICAL DISCLOSURE BULLETIN, vol. 33, no. 5, 1 October 1990 (1990-10-01), pages 187/188, XP000107428 *
KRAUSCH G ET AL: "Surface modification in the optical near field", MICROELECTRONIC ENGINEERING, vol. 32, no. 1, September 1996 (1996-09-01), pages 219-228, XP004013435 *
SIGRIST H ET AL: "SURFACE IMMOBILIZATION OF BIOMOLECULES BY LIGHT", OPTICAL ENGINEERING, vol. 34, no. 8, 1 August 1995 (1995-08-01), pages 2339 - 2348, XP000518229 *

Also Published As

Publication number Publication date
WO1998058293A2 (en) 1998-12-23
DE19726634A1 (en) 1998-12-24
AU8538798A (en) 1999-01-04

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