WO1998058074A3 - Assay methods and compositions useful for measuring receptor ligand binding - Google Patents

Assay methods and compositions useful for measuring receptor ligand binding Download PDF

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Publication number
WO1998058074A3
WO1998058074A3 PCT/CA1998/000581 CA9800581W WO9858074A3 WO 1998058074 A3 WO1998058074 A3 WO 1998058074A3 CA 9800581 W CA9800581 W CA 9800581W WO 9858074 A3 WO9858074 A3 WO 9858074A3
Authority
WO
WIPO (PCT)
Prior art keywords
receptor
cells
provides
ion influx
ligand binding
Prior art date
Application number
PCT/CA1998/000581
Other languages
French (fr)
Other versions
WO1998058074A2 (en
Inventor
Barbara Ann Ballyk
Roman Zastawny
David K H Lee
Lidia Demchyshyn
Concettina Catalano
Original Assignee
Allelix Biopharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Allelix Biopharma filed Critical Allelix Biopharma
Priority to EP98929167A priority Critical patent/EP0988395A2/en
Priority to CA002293254A priority patent/CA2293254A1/en
Priority to AU79023/98A priority patent/AU7902398A/en
Publication of WO1998058074A2 publication Critical patent/WO1998058074A2/en
Publication of WO1998058074A3 publication Critical patent/WO1998058074A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9406—Neurotransmitters
    • G01N33/9413—Dopamine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9406—Neurotransmitters
    • G01N33/942—Serotonin, i.e. 5-hydroxy-tryptamine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention provides a system for screening chemical compounds to identify ligands for receptors including G-protein coupled receptors. The invention exploits cells in which the receptor is coupled through a second messenger system to an ion channel that is gated by cyclic nucleotide. Receptor stimulation causes the second messenger system to produce cyclic nucleotide, which results in ion influx through the channel. By measuring ion influx fluorescently, the invention provides a rapid and convenient means for identifying receptor ligands. By providing mixed cell cultures that include cells expressing different receptor types, and by loading into those cells different fluorescent reporters of ion influx, the invention further provides a multiplexed system that accelerates the ligand identification process. Cells useful in the process, and mehtods for exploiting them, are described.
PCT/CA1998/000581 1997-06-13 1998-06-12 Assay methods and compositions useful for measuring receptor ligand binding WO1998058074A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP98929167A EP0988395A2 (en) 1997-06-13 1998-06-12 Assay methods and compositions useful for measuring receptor ligand binding
CA002293254A CA2293254A1 (en) 1997-06-13 1998-06-12 Assay methods and compositions useful for measuring receptor ligand binding
AU79023/98A AU7902398A (en) 1997-06-13 1998-06-12 Assay methods and compositions useful for measuring receptor ligand binding

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87466397A 1997-06-13 1997-06-13
US08/874,663 1997-06-13

Publications (2)

Publication Number Publication Date
WO1998058074A2 WO1998058074A2 (en) 1998-12-23
WO1998058074A3 true WO1998058074A3 (en) 1999-04-01

Family

ID=25364286

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1998/000581 WO1998058074A2 (en) 1997-06-13 1998-06-12 Assay methods and compositions useful for measuring receptor ligand binding

Country Status (4)

Country Link
EP (1) EP0988395A2 (en)
AU (1) AU7902398A (en)
CA (1) CA2293254A1 (en)
WO (1) WO1998058074A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1088229A4 (en) * 1998-06-25 2002-05-08 Caliper Techn Corp High throughput methods, systems and apparatus for performing cell based screening assays
ATE386815T1 (en) 2000-01-06 2008-03-15 Caliper Life Sciences Inc METHODS AND SYSTEMS FOR MONITORING INTRACELLULAR BINDING REACTIONS
DE10008373C2 (en) * 2000-02-23 2002-11-28 Helmut Adelsberger Method and device for determining ion channel activity
DE10138876B4 (en) 2001-08-08 2008-11-06 Forschungszentrum Jülich GmbH Genetically modified cyclic nucleotide-driven ion channels and their use
US7238213B2 (en) 2001-10-26 2007-07-03 Atto Bioscience Cell-based assays employing voltage and calcium dyes
US7115377B2 (en) 2001-10-26 2006-10-03 Atto Bioscience, Inc. Cell-based assays for G-protein-coupled receptor-mediated activities
US7166463B2 (en) 2001-11-16 2007-01-23 The Regents Of The University Of Colorado Nucleic acids encoding modified olfactory cyclic nucleotide gated ion channels
ATE554099T1 (en) * 2003-10-30 2012-05-15 Atto Bioscience Inc CELL-BASED ASSAY USING VOLTAGE AND CALCIUM DYES
GB0800938D0 (en) 2008-01-18 2008-02-27 Ge Healthcare Uk Ltd Multiplex cell signalling assay

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993013423A1 (en) * 1991-12-20 1993-07-08 The Salk Institute Biotechnology/Industrial Associates, Inc. Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
WO1994020617A2 (en) * 1993-03-08 1994-09-15 The Salk Institute Biotechnology/Industrial Associates, Incorporated Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
WO1994028131A1 (en) * 1993-05-21 1994-12-08 California Institute Of Technology Dna encoding inward rectifier, g-protein activated, mammalian, potassium kga channel and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993013423A1 (en) * 1991-12-20 1993-07-08 The Salk Institute Biotechnology/Industrial Associates, Inc. Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
WO1994020617A2 (en) * 1993-03-08 1994-09-15 The Salk Institute Biotechnology/Industrial Associates, Incorporated Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
WO1994028131A1 (en) * 1993-05-21 1994-12-08 California Institute Of Technology Dna encoding inward rectifier, g-protein activated, mammalian, potassium kga channel and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DHALLAN RS ET AL: "Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons.", NATURE, SEP 13 1990, 347 (6289) P184-7, ENGLAND, XP002082399 *
KARSCHIN A ET AL: "Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.", PROC NATL ACAD SCI U S A, JUL 1 1991, 88 (13) P5694-8, UNITED STATES, XP002082398 *
PATIL PG ET AL: "Elementary events underlying voltage-dependent G-protein inhibition of N-type calcium channels.", BIOPHYS J, NOV 1996, 71 (5) P2509-21, UNITED STATES, XP002082397 *
VASUDEVAN S ET AL: "Muscarinic acetylcholine receptor produced in recombinant baculovirus infected Sf9 insect cells couples with endogenous G-proteins to activate ion channels.", FEBS LETT, OCT 12 1992, 311 (1) P7-11, NETHERLANDS, XP002082396 *

Also Published As

Publication number Publication date
AU7902398A (en) 1999-01-04
EP0988395A2 (en) 2000-03-29
WO1998058074A2 (en) 1998-12-23
CA2293254A1 (en) 1998-12-23

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