WO1998049308A1 - Ngf variants - Google Patents
Ngf variantsInfo
- Publication number
- WO1998049308A1 WO1998049308A1 PCT/US1998/008242 US9808242W WO9849308A1 WO 1998049308 A1 WO1998049308 A1 WO 1998049308A1 US 9808242 W US9808242 W US 9808242W WO 9849308 A1 WO9849308 A1 WO 9849308A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ngf
- variant
- trkc
- ngf variant
- binding
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/48—Nerve growth factor [NGF]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Definitions
- This application relates to proteins which are involved in the growth, regulation or maintenance of nervous tissue, particularly neurons.
- NGF variants that have activities of another neurotrophic factor NT-3.
- NGF variants which have trkC-binding activity and trkC-signal inducing activity are provided.
- the variants optionally have trkA or trkB binding and signal induction activity.
- the NGF variants of the present invention are useful in the treatment of neuronal disorders. Nucleic acids and expression vectors encoding the NGF variant neurotrophins are also provided.
- neurotrophins form a highly homologous family of growth factors that are important for survival and maintenance of neurons during developmental and adult stages of the vertebrate nervous system (for review see 99). Limited production of neurotrophins results in death of superfluous neurons (for reviews, see (1); (2)).
- the various neurotrophins differ functionally in their ability to support survival of distinct neuronal populations in the central and the peripheral nerve system (3), (4); (5), (80).
- the neurotrophin family is a highly homologous family which includes NT-3 (6, 7, 5, 8, 9, 10), nerve growth factor (NGF) (1 1, 12), brain-derived neurotrophic factor (BDNF) (13, 14) and neurotrophin 4/5 (NT-4/5) (15, 16, 17) and neurotrophin-6 (NT-6) (91, 92).
- NGF neurotrophin/trkA
- BDNF neurotrophin/trkB
- NT-3/trkC neurotrophin-binds to trkB
- TrkA and trkB can respond in vitro under certain circumstances to multiple neurotrophins (6); (23). TrkC responds exclusively to NT-3 (25); (26).
- NT-3 signals preferably through trkC but can also bind to trkA and trkB with lower affinity (25; 101) ( Figure 1).
- the most stringent member of the trk receptors in terms of specificity interacts exclusively with the most promiscuous ligand (NT-3) of the neurotrophin family.
- the neuronal environment does restrict trkA and trkB in their ability to respond to non-preferred neurotrophic ligands (29).
- the neurotrophins can also bind to a different class of receptor termed the p75 low affinity NGF receptor (p75; (30); (31)) which has an unknown mechanism of transmembrane signaling but is structurally related to a receptor gene family which includes the tumor necrosis factor receptor (TNFR), CD40, 0X40, and CD27 (32); (33); (34), (35); (36); (37)).
- TNFR tumor necrosis factor receptor
- CD40 tumor necrosis factor receptor
- 0X40 0X40
- CD27 32
- the role of the gp75 in the formation of high-affinity binding sites and in the signal transduction pathway of neurotrophins is as yet unclear (for reviews see (38), (39))
- Nerve growth factor is a 120 ammo acid polypeptide homodime ⁇ c protein that has prominent effects on developing sensory and sympathetic neurons of the pe ⁇ pheral nervous system NGF acts via specific cell surface receptors on responsive neurons to support neuronal survival, promote neu ⁇ te outgrowth, and enhance neurochemical differentiation NGF actions are accompanied by alterations in neuronal membranes (40), (41), in the state of phosphorylation of neuronal proteins (42), (43), and in the abundance of certain mRNAs and proteins likely to play a role in neuronal differentiation and function (see, for example (44))
- Forebram cholmergic neurons also respond to NGF and may require NGF for trophic support (45) Indeed, the distribution and ontogenesis of NGF and its receptor in the central nervous system (CNS) suggest that NGF acts as target-derived neurotrophic factor for basal forebram cholmergic neurons (46), (81)
- NT-3 transcription has been detected in a wide array of peripheral tissues (e g kidney, liver, skin) as well as in the central nerve system (e g cerebellum, hippocampus) (5), (7), (82) During development, NT- 3 mRNA transcription is most prominent in regions of the central nervous system in which proliferation, migration and differentiation of neurons are ongoing (50) Supporting evidence for a role in neuronal development includes the promoting effect of NT-3 on neural crest cells (51 ) and the stimulation of the proliferation of o godendrocyte precursor cells in vivo (79) NT-3 also supports in vitro the survival of sensory neurons from the nodose ganglion (NG) (7), (5), (83) and a population of muscle sensory neurons from dorsal root ganglion (DRG) (52) In addition to these in vitro studies, a recent report showed that NT-3 prevents in vivo the degeneration of adult central noradrenergic neurons of the locus coerulus in a model that resembles the pattern of cell loss found
- trkC discrimination is unclear, especially since the most important residue in NT-3 involved in binding to trkC, R103, is conserved in all neurotrophins.
- the elucidation of the structural determinants for neurotrophin specificity is important for understanding the function and evolution of this family of growth factors.
- administration of neurotrophins in models of nerve lesions have been shown to be beneficial for regeneration and survival of neurons (97; 103). Since the neurotrophins have become candidates for therapeutics for a variety of neurodegenerative diseases, knowledge of the structural mechanism of neurotrophic specificity and function will help develop novel neurotrophin-based therapeutics.
- Neuronal populations involved in neurodegenerative disorders may express more than one trk receptor and therefore administration of molecules with multiple specificities, such as MNTS-1 (101) or PNT-1 (55) could be advantageous compared to administration of a single monospecific neurotrophin or a cocktail of monospecific neurotrophins.
- MNTS-1 (101) or PNT-1 (55) could be advantageous compared to administration of a single monospecific neurotrophin or a cocktail of monospecific neurotrophins.
- the various members of the neurotrophin family may have different pharmacokinetics and therefore the behavior of neurotrophin cocktails could be difficult to predict or control.
- neurotrophic molecules that have more than one neurotrophin activity and/or have improved pharmacokinetic properties and that are readily administered and retain effectiveness are provided by the molecules and methods presented herein.
- the present invention is based in part on the discovery that certain residues that are part of the central ⁇ -strand bundle of NT-3 and are not conserved among the neurotrophins can impart trkC-binding and trkC- signal inducing activity when grafted onto NGF.
- Exchange of NGF residues at positions 18, 20, 23, 29, 84 and 86 by their NT-3 counterparts resulted in NGF variants that bound to trkC, while maintaining their affinity to trkA, and were able to induce autophosphorylation and differentiation of PC 12 cells expressing trkC.
- NGF variants show that the amino acid at position 23 (Glycine in NGF/ Threonine in NT-3) is critical for trkC recognition while other residues fine tune the specificity of NT-3 for trkC.
- the results demonstrate the importance of non-conserved residues of the central ⁇ -strand bundle region for the interaction of NT-3 with trkC and emphasize the different mechanism of specificity determination that is employed in the NT-3/trkC and NGF/trkA ligand/receptor pairs.
- NGF variants are provided that have trkC-binding and signal inducing activity.
- the NGF variants optionally have trkA-binding and signal induction activity and optionally have trkB-binding and signal inducing activity.
- the variant has both trkA and trkC activity.
- the variant has trkC activity but lacks trkA activity.
- the amino acid sequence of the NGF variants are derived by the substitution, insertion or deletion of one or more amino acids of a parent NGF amino acid sequence, which is typically a native NGF sequence.
- the NGF is a naturally-occurring mammalian NGF. Most preferably it is a human NGF.
- An NGF variant will typically retain at least 75% amino acid sequence identity with the NGF parent molecule from which it is derived. Useful quantities of these NGF variants are provided using recombinant DNA techniques.
- An additional aspect of the present invention provides methods for producing the NGF variants, including methods using nucleic acid, vectors and host cells of the invention.
- a host cell transformed with an expression vector containing a nucleic acid encoding an NGF variant is cultured to allow expression of the nucleic acid to produce a recombinant NGF variant.
- methods and compositions for treating neuronal disorders of a mammal are provided, which use the NGF variants of the invention.
- neuronal populations involved in neurodegenerative disorders may express more than one trk receptor
- administration of molecules with multiple specificities can be advantageous compared to administration of a single monospecific neurotrophin or a cocktail of monospecific neurotrophins.
- the various members of the neurotrophin family may have different pharmacokinetics, and therefore, the behavior of neurotrophin cocktails could be difficult to predict or control, in contrast to the NGF variants herein.
- Nerve lesion models are available for examining neurotrophin activity in the regeneration and survival of neurons (97; 103).
- Figure 1 is a schematic showing specificities of neurotrophin/trk receptor interactions.
- Figure 2 depicts a sequence alignment of human NGF and human NT-3. Residue numbers that refer to the NGF sequence are used throughout this appliciation. Asterisks highlight NGF residues which were mutated in the variants analyzed in this study. Bars indicate locations of ⁇ -strands in the X-ray structure of murine NGF (59).
- Figure 3 A depicts a model of human NT-3 and Figure 3B shows the crystal structure of murine NGF.
- the two monomers of each neurotrophin are shown in tan and gray; residue numbers in NGF gray monomer are denoted by a *.
- residue numbers in NGF gray monomer are denoted by a *.
- sidechain oxygen atoms are red and sidechain nitrogen atoms are blue.
- Residue 103 (Arg in both NGF and NT-3) is purple.
- NGF residues which were replaced with their NT-3 counterparts and affected binding and specificity are yellow; residues which did not affect binding and specificity are green.
- the first residue seen in the NGF crystal structure (residue 10) is brown.
- the variable ⁇ -hairpin loop (residues 40-49) previously proposed to affect specificity (94) is shown in cyan.
- Figures 4A and 4B depict tyrosine phosphorylation of trkA in PC 12 cells expressing rat trkC.
- Cells were treated with 100 ng/ml of respective neurotrophin for 5 min. Lysates were equalized for cell protein, immunoprecipitated with an anti-trkA specific polyclonal antiserum and electrophoresed on 7.5% SDS- polyacryamide gels. Tyrosine phosphorylation was detected using an anti-phosphotyrosine mAb 4G10.
- NGF/P purified NGF
- NGF/U concentrated supernatant of NGF-expressing 293 cells
- NT-3/P purified NT-3
- NT-3/U concentrated supernatant of NT- 3 expressing 293 cells
- Figures 4A and 4B show results from two separate experiments using the neurotrophins listed above each lane.
- Figures 5A, 5B and 5C depict tyrosine phosphorylation of trkC in PC12 cells expressing rat trkC.
- Cells were treated with 100 ng/ml of respective neurotrophin for 5 min. Lysates were equalized for cell protein, immunoprecipitated with an anti-trkC specific antiserum 656 and electrophoresed on 7.5% SDS-polyacryamide gels. Tyrosine phosphorylation was detected using an anti-phosphotyrosine mAb 4G10.
- NGF/P purified NGF
- NGF/U concentrated supernatant of NGF-expressing 293 cells
- NT-3/P purified NT-3
- NT-3/U concentrated supernatant of NT-3 expressing 293 cells
- Figures 5A, 5B and 5C show results from three separate experiments using the neurotrophins listed above each lane.
- the identification of an amino acid residue is the single letter amino acid code followed by the position number of the residue. It is to be understood that the position number corresponds to the particular neurotrophin backbone; thus, D15A NT3 means that the aspartic acid at position 15 of NT3 is changed to an alanine. This aspartic acid, found within a "constant region" as defined below, corresponds to position D16 of NGF, since NGF has an additional amino acid at its N-terminus.
- the present invention provides neurotrophic NGF variants having trkC-binding and signal inducing activity.
- a neurotrophin is a protein involved in the development, regulation and maintenance of the nervous system, and in particular of neurons.
- nerve growth factor NGF
- NT3 neurotrophin-3
- NT4 neurotrophin-4
- BDNF brain-derived neurotrophic factor
- CNTF ciliary neurotrophic factor
- NGF variant herein is meant a neurotrophin which, unlike naturally occurring NGF, has neurotrophin specificity of NT-3 for trkC binding and trkC signal induction. That is, the variant contains changes that confer or impart these NT-3 activities. In one embodiment, this means that the NGF variant of the present invention will bind to at least trkC, and optionally to variety of neurotrophic receptors, preferably trkA and/or trkB.
- NGF which is the natural or native ligand for the trkA receptor
- NGF does not bind appreciably to either the trkB or trkC receptor with high affinity; for example, NGF binds to these receptors with a 500-3000 fold higher K D than BDNF or NT-3, respectively.
- an NGF variant i.e. a neurotrophin whose amino acid backbone is based on NGF, can bind to at least the trkC.
- the NGF variant binds to trkC and trkA, but not trkB.
- a preferred embodiment binds trkC but not trkA or trkB.
- the variant binds trkA, trkB, and trkC, as well as the p75 receptor. In another embodiment, the variant binds trkC and trkB, but not trkA.
- the NGF variant binds to receptors other than trkA with affinities higher than normally found for NGF, affinities substantially that of the natural ligand of that receptor.
- NGF binds strongly to trkA, and very weakly or not at all to trkB and trkC.
- one NGF variant embodiment binds to trkA with its normal or substantially normal binding affinity, and binds to either trkC with an affinity substantially similar to the trkC natural ligand, NT-3, or to trkB with an affinity similar to the trkB natural ligands BDNF or NT4/5, or both.
- the NGF variant for neurotrophin receptor exhibits a binding affinity for trkC that is no more than about 50-fold reduced compared to NT-3 for trkC, preferably less than about 20-fold reduced, more preferably less than about 15-fold reduced, even more preferably less than about 10-fold reduced, and yet even more preferrably less than about 5 fold reduced affinity than NT-3 for trkC.
- the NGF variant will have about 3 fold less affinity for trkC than does NT-3.
- NGF variants can have the same or substantially the same affinity as NT-3, or they can have less than or equal to about ten fold higher activity than NT-3.
- affinity comparisons are also applied to NGF variant trkB-binding in comparison to BDNF.
- This affinity is measured by a variety of ways, as will appreciated by those skilled in the art.
- the preferred method is the use of competition assays, as shown in (84) and in the Examples.
- binding affinities are reported as IC 50 , that is, the concentration of unlabeled competitor which inhibits 50% of the binding of labeled ligand to the receptor.
- the neurotrophin activity is measured not by binding affinity to neurotrophin receptors, but rather by the neuronal survival or neurite outgrowth assays. This indicates the ability of a variant to induce trk cellular signaling.
- all neurotrophins support the survival of embryonic neural crest-derived sensory neurons (77), (78), (7), (17). Survival of embryonic sympathetic neurons is only supported by NGF, while survival of placode-derived sensory neurons is supported by NT-3 and BDNF (85). Survival of sensory neurons of the dorsal root ganglion is supported by both NGF and BDNF (13).
- NT-3 elicits neurite outgrowth of sensory neurons from dorsal root ganglion, sympathetic chain ganglia, and nodose ganglion, as well as supports survival of nodose ganglia neurons and dorsal root ganglion neurons.
- neuronal survival assays or neurite outgrowth assays can be run to determine the activity of the NGF variant neurotrophins. Survival of motoneurons is another preferred NGF variant activity.
- the activity of an NGF variant is determined by its ability to stimulate differentiation of PC12 cells expressing trkC.
- PC12 cells expressing trkC (26; 100) can be plated onto collagen- coated tissue culture dishes, and assayed for the proportion of neurite-bearing cells.
- a neurite bearing cell is defined as one containing processes at least twice the length of the cell body after 3 days.
- PC 12 cells expressing trkB can be used to test for trkB signal inducing activity
- PC 12 cells ekpressing trkA can be used to test for trkA signal inducing activity.
- a preferred NGF variant will achieve a neurite outgrowth at least about 25% the maximal response of NT-3, more preferably about 50%, even more preferably about 75%, yet even more preferably about 90%, and most preferably about 100% maximal NT-3 response, when compared at the same concentration in the medium, and most preferably when compared at the concentration of NT-3 needed for maximal response.
- preferred comparisons are made at 1 nanogram of factor per ml of medium.
- neurotrophin specificity is determined by the neurotrophin receptor binding, and the neuronal survival assays and/or neurite outgrowth assays.
- an NGF variant as defined herein is a neurotrophin NGF which exhibits at least the binding characteristics, neuronal survival assay specificity, or the neurite outgrowth assay specificity of NT-3.
- a NGF variant with BDNF or NT4/5 specificity exhibits at least the binding characteristics, neuron survival assay specificity, or neurite outgrowth assay specificity of BDNF or NT4/5, respectively, with the preferences as discussed for NT-3 activity, e.g., neurite outgrowth at least about 25% the maximal response of BDNF, more preferably about 50%, etc.
- NGF variants are that have trkC-binding and signal inducing activity will typically retain at least 75% amino acid sequence identity with the NGF parent molecule from which it is derived. In other embodiments, the NGF variant will retain preferably at least 80% identity, more preferably at least 90% identity, and most preferably at least 95% identity with the NGF parent molecule from which it is derived.
- NGF variant of the invention can be derived from NGF by substituting at amino acid positions G23, H84, and either V 18 or V20 (or both) to impart or recruit trkC binding in order to obtain an NGF variant that binds trkC and induces receptor signaling.
- V20 is substituted.
- the NGF variant can further contain a substitution of F86, T81 , or T29, which can enhance or fine tune NT-3 specificity.
- Preferred NGF variants include NGF 130, NGF 131, NGFR2, and NGFR3, which have substitutions in the human NGF sequence as provided in the Examples (see Table 3).
- substitutions at these positions are G23T, H84Q, V18E, V20L, F86Y, T81K, and T29I.
- other substitutions at these positions can be made that recruit trkC binding and signal-inducing activity, for example, conservative and homolog series substitutions.
- V 18 can be substituted with an amino acid substitution that is conservative to or a homolog of the preferred substitution-glutamic acid-such as with aspartic acid or glutamine to enhance trkC binding.
- V20 can be substituted with threonine or a larger hydrophobic amino acid (isoleucine, methionine) to enhance trkC binding.
- G23 can be substituted with serine or alanine.
- T29 can be substituted with isoleucine, valine, or leucine.
- T29I is present when F86Y is present.
- H84 can be substituted with asparagine or glutamine.
- F86 can be substituted with methionine or tryptophan to effect trkC binding.
- the native NGF amino acids in the central ⁇ -strand bundle which have not been substituted to recruit substantiol trkC binding, are preferably retained when.
- valine at position 18 can be conservatively substituted with leucine or threonine
- V20 can be substituted with alanine
- T29 can be substituted with serine
- T81 can be substituted with arginine, isoleucine, valine, leucine or serine.
- Y79 can be substituted with glutamine, phenylalanine, methionine, isoleucine, leucine, tryptophan, or asparagine, but preferably remains Y79.
- the NGF variant can further contain a modification to the NGF sequence that recruits or imparts trkB binding to yield an NGF variant that also binds trkB in addition to trkC.
- the modification can be any chemical change to the molecule.
- a modification includes substitutions, insertions, deletions, and chemical modifications including but not limited to deamidation, acetylation, acylation, PEGylation, phosphorylation, myristylation, and oxidation.
- a preferred modification is an amino acid substitution at D16.
- a preferred variant has D16A substitution. WO 95/33829, published December 14, 1995, which discloses D16A NGF, is incorporated herein by reference.
- BDNF BDNF
- BDNF has a number of domains which appear to confer BDNF specificity.
- SKKRIG substitution of the BDNF sequence from positions 93-99
- NGF NGF
- NGF has a number of domains which can affect NGF specificity when modified.
- the N-terminal amino acids of NGF are the main region in NGF responsible for trkA binding. Significant losses of biological activity and receptor binding were observed with purified homodimers of human and mouse NGF, representing homogenous truncated forms modified at the amino and carboxy termini.
- the 109 amino acid species (10- 118)hNGF, resulting from the loss of the first 9 residues of the N-terminus and the last two residues from the C- terminus of purified recombinant human NGF, is 300-fold less efficient in displacing mouse [ 125 I]NGF from the human trkA receptor compared to (1-118)hNGF.
- the NGF variant can contain a modification of the 10-amino-acid-N-terminal region to reduce or eliminate trkA binding.
- the 7 N-terminal amino acids (SSSHPIF) of NGF can be deleted or substituted, for example, with the N-terminal amino acids of NT-3 (YAEHKS), to obtain an NGF variant with reduced or absent trkA-binding activity.
- the exact number of NGF N-terminal residues modified is not crucial, where from about 4 to about 10 N-terminal residues may be exchanged, although in some embodiments, a single amino acid change will be sufficient.
- At least one of the 10 N-terminal amino acids are deleted or substituted to reduce eliminate trkA binding.
- a particularly preferred position for modification is the histidine at amino acid position 4, which appears to be quite important for NGF specificity, as well as the proline at position 5.
- residues of NGF have been shown to be important in NGF trkA receptor binding as well as bioactivity assays. For example, there are a number of residues which, when mutated, lose NGF activity. These residues include, but are not limited to, D30, Y52, R59, R69, and H75.
- alanine can be substituted at these positions to disrupt trkA binding
- other amino acids that are non-conservative to the amion acid at that position can also be used.
- WO 95/33829 published December 14, 1995, which discloses NGF variants that lack NGF activity, is incorporated herein by reference.
- the trkB-recruiting modification can be combined with the trkA-reducing modification to yield a variant that binds both trkC and trkB, but not trkA.
- NGF variants containing an NGF having trkC-recruiting substitutions at amino acid positions VI 8, V20, G23, H84, and either F86, Y79 or T81 or any combination of these three, wherein the variant binds trkC.
- these NGF variants are substituted at both T81 and F86 to enhance or fine tune the trkC-binding activity.
- the NGF variants can further contain a substitution of T29.
- Substitutions include, G23T, G23A, Y79Y, Y79Q, Y79F, Y79M, Y79I, Y79L, Y79W, Y79N, H84Q, H84N, V18E, V18D, V18Q, V20L, V20T, V20I, V20M, F86Y, F86M, F86W, T81K, and T29I.
- substitutions at these positions can be made that recruit trkC binding and signal-inducing activity, for example, conservative and homolog series substitutions.
- Preferred substitutions are G23T, H84Q, V18E, V20L, F86Y, T81K, and T29I, with Y preferred at position 79.
- NGF variants of this type include NGF126, NGF1234, NGF124, NGF125, NGF12, NGFR4, and NGF123, and NGF127, whose specific amino acid substitutions in human NGF are provided in Table 3 of the Examples.
- NGF is a 120 amino acid polypeptide homodimeric protein. While NGF can be produced in its 120 form, a more preferred parent or backbone form for NGF variants is the 1 18 amino acid form, preferably in dimer form. In the 118 form Rl 19 and A 120 are absent. This form can be obtained by expression using a 1 18-NGF- encoding nucleic acid or by selective post-translational proteolysis of the 120 form, e.g., with trypsin. In another embodiment the 1 17 NGF form serves as the parent or backbone NGF. A composition containing an NGF variant or variants and a physiologically acceptable carrier are also provided.
- residues in the vicinity of the residues discussed above can also enhance or fine tune NT-3 specificity.
- changes in the constant regions may also give NT3 specificity.
- mutations at positions R31 and E92 in NT-3, which cause increases in NT-3 binding to trkC, specifically, R31 A and E92A NT3 can be incorporated into the corresponding positions in NGF, using the procedures described below.
- NGF neurotrophic factor
- substitutions may be included in the NGF variant backbones to enhance NGF specificity.
- residues include, but are not limited to, El l, F12, D24, E41, N46, S47, K57, D72, N77, D105, and l 15. While alanine can be substituted at these positions to maintain or enhance trkA binding, other amino acids that are conservative to alanine or to the amino acid at that position can also be used. The following provides a guideline.
- amino acids to be substituted are chosen on the basis of characteristics understood by those skilled in the art. For example, when small alterations in the characteristics are desired, substitutions are generally made in accordance with the following table: Table 2
- substitutions that are less conservative than those shown in Table 1.
- substitutions may be made which more significantly affect: the structure of the polypeptide backbone in the area of the alteration, for example the alpha-helical or beta-sheet structure; the charge or hydrophobicity of the molecule at the target site; or the bulk of the side chain.
- substitutions which in general are expected to produce the greatest changes in the polypeptide's properties are those in which (a) a hydrophilic residue, e.g., seryl or threonyl, is substituted for (or by) a hydrophobic residue, e.g., leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain, e.g., lysyl, arginyl, or histidyl, is substituted for (or by) an electronegative residue, e.g., glutamyl or aspartyl; or (d) a residue having a bulky side chain, e.g., phenylalanine, is substituted for (or by) one not having a side chain, e.g., glycine.
- homologue-scanning mutagenesis can all be used to generate putative NGF variants which may then be screened for receptor binding using the techniques described in the Examples and well-known in the art.
- neurotrophin receptor or grammatical equivalents herein is meant a receptor which binds a neurotrophin ligand.
- the neurotrophin receptor is a member of the tyrosine kinase family of receptors, generally referred to as the "trk” receptors, which are expressed on the surface of distinct neuronal populations.
- the trk family includes, but is not limited to, trkA (also known as pl40 trk ); trkB (also known as pl45 ,rkB ); and trkC (also known as pl45? kC ).
- the neurotrophin receptor is p75 , also called p75 or low-affinity nerve growth factor receptor (LNGFR). It is to be understood that other as yet undiscovered neurotrophin receptors may also bind the NGF variant neurotrophins of the present invention, as will be easily ascertainable by those skilled in the art. In one embodiment, binding to the p75 receptor by the NGF variant has been substantially diminished or eliminated For example, there are a variety of ammo acid residues which contribute to p75 binding, m which mutations result m diminished p75 binding In NGF, mutations at positions F 12, 131, K32, K34, K50, Y52, R69,
- K74, K88, LI 12, SI 13, Rl 14, and Kl 15 all result in diminished p75 binding F12, 131, K50, Y52, R69, and K74 are all within constant regions
- NGF variants can have ammo acid substitutions, insertions or deletions compared to the wild-type sequences which do not affect trk binding but are merely variations of the sequence In some embodiments, these mutations will be found within the same positions identified as important to specificity, l e in some cases, neutral mutations may be made without changing neurotrophin specificity
- the NGF variant neurotrophins of the present invention can be made in a variety of ways, using recombinant technology
- recombinant nucleic acid herein is meant nucleic acid in a form not normally found in nature That is, a recombinant nucleic acid is flanked by a nucleotide sequence not naturally flanking the nucleic acid or has a sequence not normally found in nature
- Recombinant nucleic acids can be originally formed in vitro by the manipulation of nucleic acid by restriction endonucleases, or alternatively using such techniques as polymerase cha reaction It is understood that once a recombinant nucleic acid is made and reintroduced into a host cell or organism, it will replicate non-recombmantly, / e , using the in vivo cellular machinery of the host cell rather than in vitro manipulations, however, such nucleic acids, once produced recombinantly, although subsequently replicated non-recombmantly, are still considered
- a "recombinant protein” is a protein made using recombinant techniques, . e , through the expression of a recombinant nucleic acid as depicted above
- a recombinant protein is distinguished from naturally occurring protein by at least one or more characteristics
- the protem may be isolated away from some or all of the proteins and compounds with which it is normally associated in its wild type host
- the definition includes the production NGF variant neurotrophins from one organism in the same or different organism or host cell
- the protein may be made in the same organism from which it is derived but at a significantly higher concentration than is normally seen, e g , through the use of a inducible or high expression promoter, such that increased levels of the protein is made
- the protein may be in a form not normally found m nature, as in the addition of an epitope tag or ammo acid substitutions, msertions and deletions
- expression vectors may be either self-rephcatmg extrachromosomal vectors or vectors which integrate into a host genome
- expression vectors include transcriptional and translational regulatory nucleic acid operably linked to the nucleic acid encodmg the NGF variant neurotrophin "Operably linked" in this context means that the transcriptional and translational regulatory DNA is positioned relative to the coding sequence of the NGF variant neurotrophin in such a manner that transcription is initiated Generally, this will mean that the promoter and transcriptional initiation or start sequences are positioned 5' to the NGF variant neurotrophin coding region
- the transcriptional and translational regulatory nucleic acid will generally be appropriate to the host cell used to express the NGF variant neurotrophin; for example, transcriptional and translational regulatory nucleic acid sequences from mammalian cells will be used to express the NGF variant neurotrophin in mammalian cells.
- transcriptional and translational regulatory sequences may include, but are not limited to, promoter sequences, signal sequences, ribosomal binding sites, transcriptional start and stop sequences, translational start and stop sequences, termination and poly A signal sequences, and enhancer or activator sequences.
- the regulatory sequences include a promoter and transcriptional start and stop sequences.
- NGF variants Methods, vectors, and host cells suitable for adaptation to the synthesis of NGF variants in recombinant vertebrate cell culture are described in Gething et al, Nature, 293:620-625 (1981); Mantei et al., Nature, 281:40-46 (1979); EP 117,060; and EP 117,058.
- a particularly useful plasmid for mammalian cell culture expression of an NGF variant is pRK5 (EP 307,247), pRK7, or pSVI6B.
- WO 91/08291 published 13 June 1991, is incorporated herein by reference.
- Promoter sequences encode either constitutive or inducible promoters.
- Hybrid promoters which combine elements of more than one promoter, are also known in the art, and are useful in the invention.
- the expression vector may comprise additional elements.
- the expression vector may have two replication systems, thus allowing it to be maintained in two organisms, for example in mammalian cells for expression and in a procaryotic host for cloning and amplification.
- the expression vector contains at least one sequence homologous to the host cell genome, and preferably two homologous sequences which flank the expression construct.
- the integrating vector may be directed to a specific locus in the host cell by selecting the appropriate homologous sequence for inclusion in the vector. Constructs for integrating vectors are well known in the art.
- the expression vector contains a selectable marker gene to allow the selection of transformed host cells.
- Selection genes are well known in the art and will vary with the host cell used.
- the NGF variant neurotrophins of the invention are produced by culturing a host cell transformed with an expression vector containing nucleic acid encoding a NGF variant neurotrophin, under the appropriate conditions to induce or cause expression of the NGF variant neurotrophin.
- the conditions appropriate for NGF variant neurotrophin expression will vary with the choice of the expression vector and the host cell, and will be easily ascertained by one skilled in the art.
- the use of constitutive promoters in the expression vector will require optimizing the growth and proliferation of the host cell, while the use of an inducible or repressible promoter requires the appropriate growth conditions for induction or derepression.
- the NGF variant neurotrophin is purified or isolated after expression.
- the NGF variant neurotrophins may be isolated or purified in a variety of ways known to those skilled in the art depending on what other components are in the sample. Standard purification methods include electrophoretic, molecular, immunological and chromatographic techniques, including ion exchange, hydrophobic, affinity, and reverse-phase HPLC chromatography, and chromatofocusing. Ultrafiltration and diafiltration techniques, in conjunction with protein concentration, are also useful. For general guidance in suitable purification techniques, see (57). The degree of purification necessary will vary depending on the use of the NGF variant neurotrophin. In some instances no purification will be necessary.
- Appropriate host cells include yeast, bacteria, archebacteria, fungi such as filamentous fungi, and plant and animal cells, including mammalian cells.
- yeast yeast
- bacteria archebacteria
- fungi such as filamentous fungi
- plant and animal cells including mammalian cells.
- yeast yeast
- bacteria archebacteria
- fungi such as filamentous fungi
- plant and animal cells including mammalian cells.
- yeast yeast, bacteria, archebacteria, fungi such as filamentous fungi, and plant and animal cells, including mammalian cells.
- Saccharomyces cerevisiae and other yeasts E. coli, Bacillus subtilis, Pichia pastoris, SF9 cells, C129 cells, 293 cells, Neurospora, and CHO, COS,
- HeLa cells immortalized mammalian myeloid and lymphoid cell lines.
- a preferred host cell is a mammalian cell, and the most preferred host cells include CHO cells, COS-7 cells, and human fetal kidney cell line 293.
- the NGF variant neurotrophins of the invention are expressed in mammalian cells. Mammalian expression systems are also known in the art. Some genes may be expressed more efficiently when introns are present. Several cDNAs, however, have been efficiently expressed from vectors that lack splicing signals. Thus, in some embodiments, the nucleic acid encoding the NGF variant neurotrophin includes introns.
- the methods of introducing exogenous nucleic acid into mammalian hosts, as well as other hosts, is well known in the art, and will vary with the host cell used, and include dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, and direct microinjection of the DNA into nuclei.
- NGF variant neurotrophins are produced in yeast cells.
- yeast expression systems are well known in the art, and include expression vectors for Saccharomyces cerevisiae, Candida albicans and
- NGF variant neurotrophins are expressed in bacterial systems.
- Expression vectors for bacteria are well known in the art, and include vectors for Bacillus subtilis, E. coli, Streptococcus cremoris, and Streptococcus lividans, among others.
- the bacterial expression vectors are transformed into bacterial host cells using techniques well known in the art, such as calcium chloride treatment, electroporation, and others.
- NGF variant neurotrophins are produced in insect cells.
- Expression vectors for the transformation of insect cells, and in particular, baculovirus-based expression vectors, are well known in the art. Materials and methods for baculovirus/insect cell expression systems are commercially available in kit form; for example the "MaxBac" kit from Invitrogen in San Diego.
- Recombinant baculovirus expression vectors have been developed for infection into several insect cells.
- recombinant baculoviruses have been developed for Aedes aegypti, Autographa californica, Bombyx mori, Drosophila melangaster, Spodoptera frugiperda, and Trichoplusia ni.
- NGF variant neurotrophins are used as neurotrophic factors. These NGF variant neurotrophins may be utilized in various diagnostic and therapeutic applications. The NGF variants can also be used as animal feed.
- the NGF variant neurotrophins of the present invention are useful in diagnostic methods of detecting neurotrophin receptors.
- the NGF variant neurotrophins of the present invention may be labelled.
- a "labelled NGF variant neurotrophin” herein is meant a NGF variant neurotrophin that has at least one element, isotope or chemical compound attached to enable the detection of the NGF variant neurotrophin or the NGF variant neurotrophin bound to a neurotrophin receptor.
- labels fall into three classes: a) isotopic labels, which may be radioactive or heavy isotopes; b) immune labels, which may be antibodies or antigens; and c) colored or fluorescent dyes. The labels may be incorporated into the NGF variant neurotrophin at any position.
- the NGF variant neurotrophins are used to detect neurotrophin receptors, either in vitro or in vivo.
- the presence of neurotrophin receptors can be an indication of the presence of certain cell types, useful in diagnosis. That is, a subpopulation of certain cell types may be shown by the binding of the labelled NGF variant neurotrophin to the cells via the receptors.
- the NGF variant neurotrophins of the present invention are useful as standards in neurotrophin assays.
- the activity of a NGF variant neurotrophin in any particular assay may be determined using known neurotrophin standards, and then the NGF variant neurotrophin may be used in the diagnosis and quantification of neurotrophins.
- the NGF variant neurotrophins of the present invention are useful as components of culture media for use in culturing nerve cells in vivo, since many nerve cell cultures require growth factors.
- the NGF variant neurotrophins of the present invention can replace other neurotrophic factors which are frequently used as media components.
- the amount of the NGF variant neurotrophins to be added can be easily determined using standard assays.
- the NGF variant neurotrophins of the present invention are also useful to generate antibodies, which can be used in the diagnosis, identification, and localization of neurotrophins or neurotrophin antibodies within an organism or patient.
- the NGF variant neurotrophins can be used to make polyclonal or monoclonal antibodies as is well known by those skilled in the art.
- the antibodies can then be labelled and used to detect the presence, or absence, of the neurotrophins.
- diagnosis of neural disorders associated with neurotrophins may be detected.
- the antibodies are detected indirectly, by using a second antibody.
- primary antibodies may be made in mice or rabbits, and then labelled anti-mouse or anti-rabbit antibodies are used to detect the primary antibodies. Either of these methods, as well as similar methods well known in the art, allow the detection of neurotrophins in a variety of tissues.
- the antibodies generated to the NGF variant neurotrophins of the present invention are also useful for the purification of neurotrophins and NGF variant neurotrophins. Since generally the amino acid substitutions of the NGF variant neurotrophins are small, many immune epitopes are shared by the neurotrophins and NGF variant neurotrophins. Thus, antibodies generated to the NGF variant neurotrophins will bind naturally occurring neurotrophins, and thus are useful in purification. For example, purification schemes based on affinity chromatography techniques can be used, as are well known in the art.
- NGF variant formulations of the invention are believed to be useful in promoting the development, maintenance, or regeneration of neurons in vitro and in vivo, including central (brain and spinal chord), peripheral (sympathetic, parasympathetic, sensory, and enteric neurons), and motor neurons. Accordingly, NGF variant formulations of the invention are utilized in methods for the treatment of a variety of neurologic diseases and disorders. In a preferred embodiment, the formulations of the present invention are administered to a patient to treat neural disorders.
- neural disorders herein is meant disorders of the central and/or peripheral nervous system that are associated with neuron degeneration or damage.
- neural disorders include, but are not limited to, Alzheimer's disease, Parkinson's disease, Huntington's chorea, stroke, ALS, peripheral neuropathies, and other conditions characterized by necrosis or loss of neurons, whether central, peripheral, or motor neurons, in addition to treating damaged nerves due to trauma, burns, kidney disfunction, injury, and the toxic effects of chemotherapeutics used to treat cancer and AIDS.
- peripheral neuropathies associated with certain conditions such as neuropathies associated with diabetes, AIDS, or chemotherapy may be treated using the formulations of the present invention. It also is useful as a component of culture media for use in culturing nerve cells in vitro or ex vivo.
- NGF variant formulations are administered to patients in whom the nervous system has been damaged by trauma, surgery, stroke, ischemia, infection, metabolic disease, nutritional deficiency, malignancy, or toxic agents, to promote the survival or growth of neurons, or in whatever conditions are treatable with NGF, NT-3, BDNF or NT4-5.
- the treatment or effect is dependent on the particular trk-binding function or functions present in the NGF variant.
- NGF variant formulation of the invention can be used to promote the survival or growth of motor neurons that are damaged by trauma or surgery.
- NGF variant formulations of the invention can be used to treat motoneuron disorders, such as amyotrophic lateral sclerosis (Lou Gehrig's disease), Bell's palsy, and various conditions involving spinal muscular atrophy, or paralysis.
- NGF variant formulations of the invention can be used to treat human neurodegenerative disorders, such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, Huntington's chorea, Down's Syndrome, nerve deafness, and Meniere's disease.
- NGF variant formulations of the invention can be used as cognitive enhancer, to enhance learning particularly in dementias or trauma.
- Alzheimer's disease which has been identified by the National Institutes of Aging as accounting for more than 50% of dementia in the elderly, is also the fourth or fifth leading cause of death in Americans over 65 years of age. Four million Americans, 40% of Americans over age 85 (the fastest growing segment of the U.S. population), have Alzheimer's disease. Twenty-five percent of all patients with Parkinson's disease also suffer from Alzheimer's disease-like dementia. And in about 15% of patients with dementia, Alzheimer's disease and multi-infarct dementia coexist. The third most common cause of dementia, after Alzheimer's disease and vascular dementia, is cognitive impairment due to organic brain disease related directly to alcoholism, which occurs in about 10% of alcoholics.
- Alzheimer's disease As well as for vascular dementia and cognitive impairment due to organic brain disease related to alcoholism, is the degeneration of the cholinergic system arising from the basal forebrain (BF) to both the codex and hippocampus (Bigl et al. in Brain Cholinergic Systems. M. Steriade and D. Biesold, eds., Oxford University Press, Oxford, pp.364-386 (1990)). And there are a number of other neurotransmitter systems affected by Alzheimer's disease (Davies Med. Res. Rev.3:22 ⁇ (1983)). However, cognitive impairment, related for example to degeneration of the cholinergic neurotransmitter system, is not limited to individuals suffering from dementia.
- NGF variant formulations of the invention are preferably used to treat neuropathy, and especially peripheral neuropathy.
- Peripheral neuropathy refers to a disorder affecting the peripheral nervous system, most often manifested as one or a combination of motor, sensory, sensorimotor, or autonomic neural dysfunction.
- the wide variety of morphologies exhibited by peripheral neuropathies can each be attributed uniquely to an equally wide number of causes.
- peripheral neuropathies can be genetically acquired, can result from a systemic disease, or can be induced by a toxic agent. Examples include but are not limited to diabetic peripheral neuropathy, distal sensorimotor neuropathy, or autonomic neuropathies such as reduced motility of the gastrointestinal tract or atony of the urinary bladder.
- neuropathies associated with systemic disease include post-polio syndrome or AIDS-associated neuropathy; examples of hereditary neuropathies include Charcot-Marie-Tooth disease, Refsum's disease, Abetalipoproteinemia, Tangier disease, Krabbe's disease, Metachromatic leukodystrophy, Fabry's disease, and Dejerine-Sottas syndrome; and examples of neuropathies caused by a toxic agent include those caused by treatment with a chemotherapeutic agent such as vincristine, cisplatin, methotrexate, or 3'-azido-3'-deoxythymidine.
- chemotherapeutic agent such as vincristine, cisplatin, methotrexate, or 3'-azido-3'-deoxythymidine.
- a method of treating a neural disorder in a mammal comprising administering to the mammal a therapeutically effective amount of an NGF variant.
- the neural disorder is a peripheral neuropathy, more preferably diabetic peripheral neuropathy, chemotherapy- induced peripheral neuropathy, or HIV-associated neuropathy.
- the peripheral neuropathy affects motor neurons.
- the administration of NT- 3 prevents the in vivo degeneration of adult central noradrenergic neurons of the locus coerulus in a model that resembles the pattern of cell loss found in Alzheimer's disease (86)
- the addition of NT3 has been shown to enhance sprouting of corticospinal tract during development, as well as after adult spinal cord lesions (58). In fact, when NT3 was administered with antibodies which inhibit myelin-associated growth inhibitory proteins, long-distance regeneration was seen.
- the NGF variant neurotrophins of the present invention can be used in place of NT3 in this application.
- a therapeutically effective dose of a NGF variant neurotrophin is administered to a patient.
- therapeutically effective dose herein is meant a dose that produces the therapeutic effects for which it is administered. The exact dose will depend, for example, on the disorder to be treated, the mode of administration, and the clinical state of the patient to be treated, and will be ascertainable by one skilled in the art using known techniques.
- the NGF variant neurotrophins of the present invention are administered at about 1 ⁇ g/kg to about 100 mg/kg per day.
- adjustments for age as well as the body weight, general health, sex, diet, time of administration, drug interaction and the severity of the disease may be necessary, and will be ascertainable with routine experimentation by those skilled in the art.
- the compositions are prepared containing amounts of NGF variant from 0.07 to 20 mg/ml, preferably 0.08 to 15 mg/ml, more preferably 0.09 to 10 mg/ml, and most preferably 0.1 to 2 mg/ml.
- these compositions may contain from about 0.1 mg/ml to about 2 mg/ml NGF variant, corresponding to the currently contemplated NGF dosage rate for such treatment.
- NGF variant is expected to be well-tolerated, as is NGF, and higher doses can be administered if necessary as determined by the physician.
- preferred single dose IV or SC is preferably less than 1 ug/kg of NGF, although higher doses can be given when steps are taken to ameliorate the hyperalgesia and myalgias, as would be known in the art.
- a preferred regimen for peripheral neuropathy is 0.1 ug/kg, three times per week SC or 0.3 ug/kg, once weekly SC.
- a "patient” for the purposes of the present invention includes both humans and other animals and organisms. Thus the methods are applicable to both human therapy and veterinary applications.
- the administration of the NGF variant neurotrophins of the present invention can be done in a variety of ways, e.g., those routes known for specific indications, including, but not limited to, orally, subcutaneously, intravenously, intracerebrally, intranasally, transdermally, intraperitoneally, intramuscularly, intrapulmonary, vaginally, rectally, intraarterially, intralesionally, intraventricularly in the brain, or intraocularly.
- the NGF variant neurotrophins may be administered continuously by infusion into the fluid reservoirs of the CNS, although bolus injection is acceptable, using techniques well known in the art, such as pumps or implantation.
- the NGF variant neurotrophins may be directly applied as a solution or spray. Sustained release systems can be used. Generally, where the disorder permits, one should formulate and dose the NGF variant for site-specific delivery. Administration can be continuous or periodic. Administration can be accomplished by a constant- or programmable-flow implantable pump or by periodic injections. Semipermeable, implantable membrane devices are useful as means for delivering drugs in certain circumstances. For example, cells that secrete soluble NGF variant can be encapsulated, and such devices can be implanted into a patient, for example, into the brain or spinal chord (CSF) of a patient suffering from Parkinson's Disease. See, U.S. Patent No.
- CSF spinal chord
- the present invention includes an implantation device, for preventing or treating nerve damage or damage to other cells as taught herein, containing a semipermeable membrane and a cell that secretes NGF variant encapsulated within the membrane, the membrane being permeable to NGF variant, and impermeable to factors from the patient detrimental to the cells.
- the patient's own cells, transformed to produce NGF variant ex vivo could be implanted directly into the patient, optionally without such encapsulation.
- the methodology for the membrane encapsulation of living cells is familiar to those of ordinary skill in the art, and the preparation of the encapsulated cells and their implantation in patients may be accomplished readily as is known in the art.
- the secreted NGF variant is a human mature NGF backbone variant when the patient is human.
- the implants are preferably non-immunogenic and/or prevent immunogenic implanted cells from being recognized by the immune system.
- a preferred location for the implant is the cerebral spinal fluid of the spinal cord.
- compositions of the present invention comprise a NGF variant neurotrophin in a form suitable for administration to a patient.
- the pharmaceutical compositions are in a water soluble form, and may include such physiologically acceptable materials as carriers, excipients, stabilizers, buffers, salts, antioxidants, hydrophilic polymers, amino acids, carbohydrates, ionic or nonionic surfactants, and polyethylene or propylene glycol.
- the NGF variant neurotrophins may be in a time-release form for implantation, or may be entrapped in microcapsules using techniques well known in the art.
- NGF variant is preferably formulated in physiologically acceptable acetate buffer from pH 5 to pH 6, to markedly increase stability in these compositions.
- Acetate concentrations can range from 0.1 to 200 mM, more preferably from 1 to 50 mM, and even more 5 to 30 mM, and most preferably from 10 to 20 mM.
- One preferred embodiment has 20 mM acetate and another has 10 mM acetate in the solution.
- a preferred acetate salt for enhancing stability and buffering capacity is sodium acetate.
- physiologically acceptable acetate salts can be used, for example potassium acetate.
- Suitable pH ranges for the preparation of the compositions herein are from 5 to 6, preferably 5.4 to 5.9, more preferably 5.5 to 5.8.
- a preferred pH is 5.5, which enhances stability and buffering capacity.
- Another preferred embodiment is pH 5.8.
- the formulation contains a pharmaceutically acceptable salt, preferably sodium chloride, and preferably at about physiological concentrations.
- a pharmaceutically acceptable salt preferably sodium chloride
- Low concentrations are preferred, e.g., less than about 0.3 M to about .05 M, preferably from 0.16 to 0.20 M NaCl, more preferably 0.13 to 0.15 M.
- the sodium chloride concentration is 136 mM. In another preferred embodiment the concentration is 142 mM.
- the formulations of the invention can contain a pharmaceutically acceptable preservative.
- the preservative concentration ranges from 0.1 to 2.0%, typically v/v.
- Suitable preservatives include those known in the pharmaceutical arts. Benzyl alcohol, phenol, m-cresol, methylparaben, and propylparaben are preferred preservatives.
- Benzyl alcohol is a particularly preferred preservative that results in enhanced NGF stability.
- a particularly preferred benzyl alcohol concentration is 0.7 to 1.2%, more preferably 0.8 to 1.0%, with a particularly preferred concentration of 0.9%.
- the formulations of the invention can include a pharmaceutically acceptable surfactant.
- Preferred surfactants are non-ionic detergents.
- Preferred surfactants include Tween 20 and pluronic acid (F68). F68 is particularly preferred for enhancing NGF variant stability.
- Suitable surfactant concentrations are 0.005 to 0.02%.
- a preferred concentration for surfactant is 0.01%.
- Surfactants are used to minimize particulate formation.
- the composition contains an NGF variant concentration of 0.1 mg/ml, a sodium acetate concentration of 20 mM, pH 5.5, a sodium chloride concentration of 136 mM, and benzyl alcohol concentration at 0.9% (v/v).
- NGF variant concentration is 2.0 mg/ml
- the sodium acetate concentration is 10 mM
- pH 5.5 a sodium chloride concentration
- benzyl alcohol concentration is 142 mM.
- kits for NGF variant administration which includes a vial or receptacle containing a pharmaceutical composition of the invention comprising a pharmaceutically effective amount of NGF variant and a pharmaceutically acceptable acetate-containing buffer.
- a preferred vial volume is one suitable for multi-dose use— allowing repeated withdrawal of sample.
- the increased stability attained with the formulations of the invention allow multi-dose liquid formulation.
- a multi-dose vial will provide sufficient formulation to supply sufficient dosage for one patient for one month, preferably one week.
- the composition volume generally ranges from 0.3 to 10.0 ml and more preferably from 1.6 to 2.0 ml, depending on dose concentration, frequency and ease of use.
- a volume of 1.8 ml is convenient when either 0 3 ug/kg or 0 1 ug/kg are used, allowing 7 or 24 doses, respectively
- a light sensitive component such as benzyl alcohol
- the vial is protected from intense light
- the vial walls can comprise light transmission reducing materials
- translucent amber or brown vials or an opaque vial can be used
- the vial contains multi-dose formulation
- a selected multi-dose liquid formulation can be filled in 3 cc Type I glass vial with 1 8 mL fill volume Selection of stopper will be based on compatibility of different types of stopper with the selected formulation
- compositions of the invention are typically stored at 2 to 8 degrees C
- the formulations are stable to numerous freeze thaw cycles as shown herein
- the formulation is prepared with the above acetate concentrations
- a preferred means of preparing a formulation is to dialyze a bulk NGF variant solution mto the final formulation buffer Final NGF variant concentrations are achieved by appropriate adjustment of the formulation with formulation buffer absent NGF variant
- compositions hereof including lyophi zed forms are prepared in general by compounding the components using generally available pharmaceutical compounding techniques, known per se Likewise, standard lyophilization procedures and equipment well-known in the art are employed
- a particular method for preparing a pharmaceutical composition of NGF variant hereof comprises employing purified (according to any standard protein purification scheme) NGF variant, preferably rhNGF variant, in any one of several known buffer exchange methods, such as gel filtration or dialysis
- the NGF variant-encoding gene constructs discussed herein can be inserted mto target cells using any method known in the art, including but not limited to transfection, electroporation, calcium phosphate/DEAE dextran methods, and cell gun
- the constructs and engineered target cells can be used for the production of transgenic animals bearing the above-mentioned constructs as transgenes, from which NGF variant-expressing target cells may be selected using the methods discussed
- NGF variant can be delivered by gene therapy using NGF variant-encoding nucleic acid Selective
- Gene therapy includes both conventional gene therapy where a lasting effect is achieved by a single treatment, and the administration of gene therapeutic agents, which involves the one time or repeated administration of a therapeutically effective DNA or mRNA
- Techniques suitable for the transfer of nucleic acid into mammalian cells in vitro include the use of liposomes, electroporation, microinjection, cell fusion, DEAE- dextran, the calcium phosphate precipitation method, etc
- the currently preferred m vivo gene transfer techniques include transfection with viral (typically retroviral) vectors and viral coat protein- posome mediated transfection (Dzau et al , Trends in Biotechnology,
- proteins which bind to a cell surface membrane protein associated with endocytosis may be used for targeting and/or to facilitate uptake, e.g. capsid proteins or fragments thereof tropic for a particular cell type, antibodies for proteins which undergo internalization in cycling, and proteins that target intracellular localization and enhance intracellular half-life.
- the technique of receptor-mediated endocytosis is described, for example, by Wu et al., J. Biol. Chem., 262:4429-4432 (1987); and Wagner et al, Proc. Natl. Acad. Sci. USA, 87:3410-3414 (1990).
- the invention provides neurotrophic molecules with the activity of NT-3 and the superior bioavailability of NGF.
- the invention provides neurotrophic molecules having unexpectedly superior pan-neurotrophic activity compared to previous factors, thus having the additional advantage of obviating any problems associated with a mixture of individual neurotrophins.
- T81 and H84 the residues analogous to NT-3 K81 and Q84 ( Figures 2, 3B), have been shown to be involved in NGF binding to trkA (55) and it is therefore possible that they contribute to the specificity.
- V 18, G23 , T81, and H84 have been shown to be involved in trkA binding.
- the other non-conserved NT-3 residue, T23 is located in an area that is conserved within each member of the neurotrophins across species but is divergent between NT-3, BDNF and NGF. Near T23 and located on the same face of the molecule are El 8 and L20 ( Figure 3A).
- NGF and NT-3 were previously cloned, sequenced and subcloned into a vector which allows for production of double and single-stranded DNA in E. coli, as well as expression of the neurotrophins in a mammalian system under control of the cytomegalovirus promoter (83). Mutagenesis on this vector was performed according to the method of Kunkel (66). After transformation into the E. coli strain XL 1- Blue (Stratagene, San Diego, CA), colonies were screened for the presence of the desired mutation by sequencing double-stranded DNA using the Sequenase version 2.0 kit (U. S. Biochemical Co ⁇ ., Cleveland, OH). The entire DNA sequence coding for the mature NGF and NGF variants was verified for all positive clones. Double- stranded DNA was isolated from XL-1 Blue with the QIAGEN DNA purification kit (Qiagen Inc., Chatsworth CA).
- Plasmid DNA containing either the NGF or variant coding sequences was introduced into the human fetal kidney cell line 293 by calcium phosphate precipitation (70). The 75% confluent cells were co-transfected with 10 ⁇ g of plasmid DNA and 1 ⁇ g of AdVA plasmid per 15 mm cell culture dish and incubated for 15 h in serum-containing medium. Then the medium was removed and exchanged with serum-free medium (PS04) supplemented with 10 mg/L recombinant bovine insulin, 1 mg/L transferrin and trace elements.
- PS04 serum-free medium
- the ELISA assay did not detect any NGF in supematants from mock transfected cells.
- a wild-type NGF expression was performed and quantified by ELISA in parallel in order to obtain a comparative wild type concentration for receptor binding studies. All variants were expressed, quantified and assayed at least twice.
- the variants NGF1 and NGF2 carried the mutations T81K H84Q and G23T/V18E/V20L, respectively.
- the five point mutations were combined in the variant NGF12.
- Receptor immunoadhesin proteins were constructed using human trkA and trkC extracellular domains fused to immunoglobulin constant domains (88). Binding assays were performed as described (88) using a 96- well plate format. The final concentration of labeled neurotrophin in each well was approximately 30 pM for trkA and trkC binding assays. Purified recombinant human NT-3, BDNF and NGF (Genentech) were iodinated as described (101). Usually, 20 ⁇ g of the neurotrophins were iodinated to specific activities ranging from 2000 - 3000 Ci/mmol. The labeled material was stored at 4° C and used within 2 weeks of preparation.
- Variants were assayed for binding affinity to the trkA and trkC receptor at least twice for each of the multiple expressions. This procedure allowed estimation of the error in affinity determination for each of the variants. All data were analyzed by applying a four-parameter fit procedure on the data set with the Kaleidagraph software package. Binding results in Table 3 are expressed as IC50mut/IC50wt ratio. IC50 is the concentration of variant resulting in 50%o inhibition of binding of native neurotrophin. In competitive displacement binding assays, the NT-3 wild type displayed an affinity of 21.0 ⁇ 4.9 pM for trkC while NGF bound to this receptor with an affinity reduced by 3587-fold compared to NT-3 (Table 3).
- the affinity to trkC was substantially increased in a synergistic manner.
- Affinities to trkA and trkC are shown relative to NGF and NT-3, respectively.
- the results for variant affinities are expressed as the average of at least four independent binding experiments using proteins from two different expressions ⁇ SD. NT-3 residues are shown in italic.
- EXAMPLE III NGF variants with mutations in the central b-strand bundle retain trkA binding
- NGFl 2 variants with increased affinity to trkC Comparison of the model of human NT-3 (101) and the X-ray structure of mouse NGF (59) revealed several residues close to the main specificity determinants that differ between the two molecules. In order to further increase the affinity of NGF12 to trkC, some of these residues were changed to the analogous NT-3 amino acids. Adding the changes F54Y/K57R to NGF12 did not improve trkC binding (NGF125, Table 3). In contrast, addition of Y79Q/F86Y did enhance binding 2-fold compared to NGF12 (NGF124, Table 3).
- variant NGFl 31 contained the five changes V20L, G23T, V18E, T29I, H84Q and F86Y and bound to trkC with an affinity that is only 3.3-fold reduced compared to NT-3. These changes did not affect trkA binding (Table 3). NGF126 and NGFl 31 bind equally well to trkC and trkA. Both variants have the mutations V20L, G23T, H84Q and F86Y in common; NGF126 has the additional mutations V18E and T81K while NGF131 has the additional mutation T29I.
- NGF130 which possesses these four mutations, bound to trkC similar to NGF126 and NGF131 (Table 3).
- EXAMPLE VI NGF variants induce trkC receptor autophosphorylation
- NGF variants to stimulate trk receptor autophosphorylation on PC12 cell lines was determined. Approximately 1 x 10 7 PC12 cells (26) were treated at 37° C for 5 min with 100 ng/ml neurotrophin. NP-40 plate lysis and immunoprecipitation with an anti-trkA specific polyclonal antiserum (from Dr. Louis Reichardt, University of California, San Francisco) or anti-frkC specific polyclonal antiserum 656 (101) was performed as previously described (26). The phosphotyrosine content was analyzed by Western blot using monoclonal antibody 4G10 as previously described (23; 26). All tyrosine autophosphorylation assays were performed at least twice for each neurotrophin assayed.
- PC 12 cells that were engineered to constitutively express rat frkC respond to NT-3 by induction of strong autophosphorylation of trkC and formation of neurite extensions (26).
- Purified NGF (NGF/P) as well as supernatant of NGF-expressing 293 cells (NGF/U) resulted in a strong signal for frkA autophosphorylation (Figure 4A) but did not induce autophosphorylation of frkC ( Figure 5A).
- Purified NT-3 (NT-3/P) and supernatant of NT-3-expressing 293 cells (NT-3 U) induced autophosphorylation of trkC ( Figure 5A) but not trkA ( Figure 4A).
- NGFRI and NGFR5 were assayed for induction of autophosphorylation of PC12/trkC cells and NGFRI resulted in a very weak signal while the NGFR5 response was between that of NGF12 and NGFRI ( Figure 5B). These results correlate with the determined affinities of the variants for trkC. Variants of NGF12 (NGF123, NGF124, NGF125 andNGF1234) that further increased the affinity to trkC resulted in signals fortrkC autophosphorylation that were similar to that elicited by NT-3 ( Figure 5B).
- NGF variants induce trkC cellular signaling
- the ability of NGF variants to stimulate differentiation of PC12 cells expressing trkC was determined. This indicates the ability of a variant to induce trkC cellular signaling.
- Approximately 10 3 PC12 cells expressing trkC (26; 100) were plated onto 35 mm collagen-coated tissue culture dishes containing a total of 2 ml of medium.
- PC12/frkC cells were assayed at neurotrophin concentrations ranging from 250 pg/ml to 100 ng/ml.
- the proportion of neurite-bearing cells was determined by counting the number of cells containing processes at least twice the length of the cell body after 3 days.
- NGF 12 exhibited a dose-response which was shifted to higher values compared to native NT-3 and those variants with the poorest binding (NGFl, NGF2, NGFRI and NGFR5) did not reach maximal response even at the highest dose tested (Table 4).
- NGF 126 was as potent in neurite induction as native NT-3 (Table 4) and though variants NGF126, NGF130 and NGF131 were equivalent in trkC binding (Table 3), NGF 126 was more efficacious in inducing neurites, reaching maximal response at 1 ng/ml compared to 10 ng/ml for NGF130 and NGF131 (Table 4).
- these NGF variants could induce neurites through trkC in an environment where trkA competes with trkC for their binding.
- the NGF 12, NGFl and NGF2 variants acted similarly as NGF in inducing neurites (data not shown) as would be expected from the binding of these variants to trkA (Table 3).
- Variant 250 Dg/m 1 1 ng/ml lO ng/i nl 100 ng/ml
- a Values are the percent of counted cells that carried neurites which were at least twice the length of the cell body.
- the neurotrophins transduce their signal into the cell by interaction with the trk receptor tyrosine kinases (95).
- the neurofrophins and the trks both form highly homologous protein families. Within each family the different members probably have similar structures, but individual members of the two families interact with each other in a very specific manner. This inherent specificity of neurofrophins is necessary for their biological function and therefore information on the mechanisms of specificity determination contributes to an understanding of function and evolution of the neurotrophin family.
- Molecular modeling and alanine scanning mutagenesis of human NT-3 (101) and domain deletions/swaps of the human trks (102) determined the binding epitopes of this ligand/receptor system.
- the binding site extends around the central ⁇ -strand barrel and, in contrast to the NGF binding site for trkA, does not include residues from loops and the first six residues of the N-terminus ( Figure 3).
- Non-conserved residues that are part of the binding site include T23, K81 and Q84.
- Residue T23, together with LI 8 and E20, are located in an area which is conserved across all species within each of the members of the neurotrophin family, but is divergent between NT-3, NGF and BDNF.
- NGF 12 was able to bind to trkC, induce autophosphorylation of trkC expressed on PC 12 cells and did not lose affinity to trkA (Table 3, Figures 4, 5). As indicated herein the change from Glycine23 to Threonine dominates the recruitment effect in
- NGF12 Additional variants of NGF12 in which each of the mutated residues was individually changed back to the original NGF amino acid (i.e. E18V, L20V, T23G, K81T, Q84H) demonstrated that in NT-3 the most important determinant for trkC specificity is T23 followed by Q84 and L20.
- the change from glycine to threonine in NGF may introduce a sidechain that is critical in binding to trkC; alternatively, the G23T change might effect a change of the backbone conformation and thereby influence the conformation of sidechains in its vicinity.
- the change from histidine to glutamine removes a potentially charged residue which might be repulsive to trkC or the glutamine sidechain may be required for specific hydrogen bonding.
- the preference for leucine over valine at position 20 may be due to rather stringent spatial requirements of a hydrophobic interaction at the binding site.
- the amino acid at position 86 is important for neurotrophin specificity to trkC. Adding the NT-3 residue to NGF 12 effected a 4.5-fold improvement in binding to trkC while maintaining binding to trkA (variant NGF126, Table 3).
- Residue 86 is proximal to the most important determinant for binding to trkC, R103 ( Figure 3), further confirming the dominance of this structural region for trkC recognition.
- NGF residue 86 is a phenylalanine and in NT-3 it is a tyrosine suggesting, without limitation to any one theory, that the sidechain hydroxyl group may be involved in a specific hydrogen bond required for trkC recognition.
- residues at positions 18 and 29 may fine tune the specificity of NT-3 for trkC.
- the effect at position 29 seems to be dependent on the character of the amino acid at position 86.
- T29I was introduced into NGF12 (which has F86)
- trkC binding was slightly reduced whereas when T29I was introduced into two other variants (which have Y86) the binding was slightly improved (compare NGF 124 versus NGF1234, NGF130 versus NGF131, Table 3).
- residue 29 is proximal to the important R103 (Figure 3).
- the mutation V18E contributed only slightly to trkC binding (cf. NGF12 and NGFR2, Table 3).
- NGF126 which includes V18E, elicited neurite outgrowth equivalent to native NT-3 (i.e. reaching maximal response at 1 ng/ml)
- NGF130 required 10-fold more neurotrophin to elicit the maximal response (10 ng/ml) (Table 4).
- trkC non-cognate receptor
- trkA cognate receptor
- the major specificity determinants on NT-3 for trkC include L20, T23, Q84 and Y86.
- Residues El 8 and 129 may play a minor role in specificity.
- the six residues form two clusters: 129, Q84 and Y86 are proximal to R103, the most important residue involved in trkC binding, and El 8 and T23 are located together but distant from the R103 cluster ( Figure 3). This suggests, without limitation to any one theory, that trkC uses at least two unique, spatially distant sites to discriminate between the various neurotrophins.
- the present work establishes the importance of residues located in the central ⁇ -strand bundle for function and specificity of NT-3.
- non-conserved amino acids of NT-3-T23 and Q84 ⁇ can be substituted in NGF and recruit trkC binding.
- non-conserved residues in NT-3--E18, L20, 129, and Y86 ⁇ can also contribute to the specificity of the NGF variant/trkC interaction, though exhibiting a reduced effect compared to T23 and Q84.
- Replacing these six residues in NGF with their NT- 3 counte ⁇ arts maintains full affinity to trkA.
- NT-3 residues in the central ⁇ -strand bundle impart specificity
- NGF the N- terminal residues impart specificity.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Neurology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Neurosurgery (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54714498A JP2001527402A (en) | 1997-04-25 | 1998-04-23 | NGF mutant |
AU71546/98A AU7154698A (en) | 1997-04-25 | 1998-04-23 | Ngf variants |
CA002286558A CA2286558A1 (en) | 1997-04-25 | 1998-04-23 | Ngf variants |
IL13225198A IL132251A0 (en) | 1997-04-25 | 1998-04-23 | Ngf variants |
EP98918666A EP0977860A1 (en) | 1997-04-25 | 1998-04-23 | Ngf variants |
IL132251A IL132251A (en) | 1997-04-25 | 1999-10-06 | NGF VARIANTS HAVING AMINO ACID SUBSTITUTIONS THAT IMPART trkC BINDING |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/845,541 US6333310B1 (en) | 1994-06-03 | 1997-04-25 | NGF variants |
US08/845,541 | 1997-04-25 | ||
US84104597A | 1997-04-29 | 1997-04-29 | |
US08/841,045 | 1997-04-29 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998049308A1 true WO1998049308A1 (en) | 1998-11-05 |
Family
ID=27126232
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1998/008242 WO1998049308A1 (en) | 1997-04-25 | 1998-04-23 | Ngf variants |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0977860A1 (en) |
JP (1) | JP2001527402A (en) |
AU (2) | AU7154698A (en) |
CA (1) | CA2286558A1 (en) |
IL (1) | IL132251A0 (en) |
WO (1) | WO1998049308A1 (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1146899A1 (en) * | 1999-01-15 | 2001-10-24 | Regeneron Pharmaceuticals, Inc. | Methods of using a neurotrophin and its analogues for the treatment of gastrointestinal hypomotility disorders |
WO2001098361A2 (en) * | 2000-06-22 | 2001-12-27 | Genentech, Inc. | Agonist anti-trk-c monoclonal antibodies |
WO2002007774A2 (en) * | 2000-07-19 | 2002-01-31 | The Regents Of The University Of California | Methods for therapy of neurodegenerative disease of the brain |
US6683058B1 (en) | 1998-04-15 | 2004-01-27 | Regents Of The University Of California | Methods for therapy of neurodegenerative disease of the brain |
WO2005040335A2 (en) * | 2003-10-23 | 2005-05-06 | Monika Holmberg | A novel mutated nerve growth factor beta gene, proteins encoded thereby, and products and methods related thereto |
EP1620127A2 (en) * | 2003-03-20 | 2006-02-01 | Rinat Neuroscience Corp. | Methods for treating taxol-induced gut disorder |
WO2008006893A1 (en) * | 2006-07-13 | 2008-01-17 | Lay Line Genomics S.P.A. | Muteins of hngf, therapeutic uses and pharmaceutical compositions |
EP1901767A2 (en) * | 2005-06-10 | 2008-03-26 | Searete LLC. | Methods and systems for neural maintenance and regeneration |
IT201800009384A1 (en) * | 2018-10-11 | 2020-04-11 | Cosmo Srl | Peptide for cosmetic application |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995033829A1 (en) * | 1994-06-03 | 1995-12-14 | Genentech, Inc. | Pantropic neurotrophic factors |
-
1998
- 1998-04-23 EP EP98918666A patent/EP0977860A1/en not_active Withdrawn
- 1998-04-23 IL IL13225198A patent/IL132251A0/en unknown
- 1998-04-23 JP JP54714498A patent/JP2001527402A/en not_active Ceased
- 1998-04-23 WO PCT/US1998/008242 patent/WO1998049308A1/en not_active Application Discontinuation
- 1998-04-23 CA CA002286558A patent/CA2286558A1/en not_active Abandoned
- 1998-04-23 AU AU71546/98A patent/AU7154698A/en not_active Abandoned
-
2002
- 2002-08-01 AU AU2002300365A patent/AU2002300365B9/en not_active Expired
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995033829A1 (en) * | 1994-06-03 | 1995-12-14 | Genentech, Inc. | Pantropic neurotrophic factors |
Non-Patent Citations (5)
Cited By (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8486385B2 (en) | 1998-04-15 | 2013-07-16 | Regents Of The University Of California | Methods for therapy of neurodegenerative disease of the brain |
US6683058B1 (en) | 1998-04-15 | 2004-01-27 | Regents Of The University Of California | Methods for therapy of neurodegenerative disease of the brain |
EP1146899A4 (en) * | 1999-01-15 | 2003-03-05 | Regeneron Pharma | Methods of using a neurotrophin and its analogues for the treatment of gastrointestinal hypomotility disorders |
EP1146899A1 (en) * | 1999-01-15 | 2001-10-24 | Regeneron Pharmaceuticals, Inc. | Methods of using a neurotrophin and its analogues for the treatment of gastrointestinal hypomotility disorders |
JP2002534479A (en) * | 1999-01-15 | 2002-10-15 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | Methods of treating gastrointestinal hypomotility disorders using neurotrophins and analogs thereof |
US6656474B1 (en) | 1999-01-15 | 2003-12-02 | Regeneron Pharmaceuticals, Inc. | Methods of using a neurotrophin and its analogues for the treatment of gastrointestinal hypomotility disorders |
US7615383B2 (en) | 2000-06-22 | 2009-11-10 | Genentech, Inc. | Methods for treating neuropathy by agonist anti-trk-C monoclonal antibodies |
US7384632B2 (en) | 2000-06-22 | 2008-06-10 | Genentech, Inc. | Agonist anti-trk-C monoclonal antibodies |
WO2001098361A3 (en) * | 2000-06-22 | 2002-05-02 | Genentech Inc | Agonist anti-trk-c monoclonal antibodies |
WO2001098361A2 (en) * | 2000-06-22 | 2001-12-27 | Genentech, Inc. | Agonist anti-trk-c monoclonal antibodies |
AU2001261758B2 (en) * | 2000-07-19 | 2005-07-21 | Regents Of The University Of California | Methods for therapy of neurodegenerative disease of the brain |
WO2002007774A3 (en) * | 2000-07-19 | 2003-01-16 | Univ California | Methods for therapy of neurodegenerative disease of the brain |
WO2002007774A2 (en) * | 2000-07-19 | 2002-01-31 | The Regents Of The University Of California | Methods for therapy of neurodegenerative disease of the brain |
EP2060267A3 (en) * | 2000-07-19 | 2009-08-05 | The Regents Of The University Of California | Methods for therapy of neurodegenerative disease of the brain |
EP1620127A2 (en) * | 2003-03-20 | 2006-02-01 | Rinat Neuroscience Corp. | Methods for treating taxol-induced gut disorder |
EP1620127A4 (en) * | 2003-03-20 | 2007-04-04 | Rinat Neuroscience Corp | Methods for treating taxol-induced gut disorder |
WO2005040335A3 (en) * | 2003-10-23 | 2006-03-30 | Monika Holmberg | A novel mutated nerve growth factor beta gene, proteins encoded thereby, and products and methods related thereto |
WO2005040335A2 (en) * | 2003-10-23 | 2005-05-06 | Monika Holmberg | A novel mutated nerve growth factor beta gene, proteins encoded thereby, and products and methods related thereto |
EP1901767A2 (en) * | 2005-06-10 | 2008-03-26 | Searete LLC. | Methods and systems for neural maintenance and regeneration |
EP1901767A4 (en) * | 2005-06-10 | 2012-01-04 | Searete Llc | Methods and systems for neural maintenance and regeneration |
US8267920B2 (en) | 2005-06-10 | 2012-09-18 | The Invention Science Fund | Methods and systems for neural maintenance and regeneration |
US8309057B2 (en) | 2005-06-10 | 2012-11-13 | The Invention Science Fund I, Llc | Methods for elevating neurotrophic agents |
US8318136B2 (en) | 2005-06-10 | 2012-11-27 | The Invention Science Fund I, Llc | Methods and systems for neural maintenance and regeneration |
WO2008006893A1 (en) * | 2006-07-13 | 2008-01-17 | Lay Line Genomics S.P.A. | Muteins of hngf, therapeutic uses and pharmaceutical compositions |
IT201800009384A1 (en) * | 2018-10-11 | 2020-04-11 | Cosmo Srl | Peptide for cosmetic application |
WO2020075108A1 (en) * | 2018-10-11 | 2020-04-16 | Cosmo S.R.L. | Peptides for use in treating and preventing skin aging and photo-aging |
CN112823039A (en) * | 2018-10-11 | 2021-05-18 | 罗马联合化学中心有限责任公司简写C4T有限责任公司 | Peptides for treating and preventing skin aging and photoaging |
Also Published As
Publication number | Publication date |
---|---|
CA2286558A1 (en) | 1998-11-05 |
JP2001527402A (en) | 2001-12-25 |
AU2002300365B2 (en) | 2006-07-13 |
EP0977860A1 (en) | 2000-02-09 |
AU2002300365B9 (en) | 2007-03-15 |
IL132251A0 (en) | 2001-03-19 |
AU2002300365A1 (en) | 2003-01-30 |
AU7154698A (en) | 1998-11-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6333310B1 (en) | NGF variants | |
US6365373B2 (en) | Nucleic acids encoding NGF variants | |
US8101571B2 (en) | Treatment methods using NGF variants | |
Urfer et al. | The binding epitopes of neurotrophin‐3 to its receptors trkC and gp75 and the design of a multifunctional human neurotrophin. | |
Battleman et al. | HSV-1 vector-mediated gene transfer of the human nerve growth factor receptor p75hNGFR defines high-affinity NGF binding | |
Ip et al. | Ciliary neurotrophic factor and its receptor complex | |
US7119066B2 (en) | Modified ciliary neurotrophic factor (CNTF) | |
CN101123978A (en) | Neublastin variants | |
AU2002300365B2 (en) | NGF Variants | |
US7144983B1 (en) | Pantropic neurotrophic factors | |
AU677979B2 (en) | Multifunctional neurotrophic factors | |
EP0589961B1 (en) | Assay systems for neurotrophin activity | |
US6866851B1 (en) | GFRα1-RET specific agonists and methods therefor | |
Vantini | The pharmacological potential of neurotrophins: a perspective | |
Cuello | Neurotrophins and neurodegenerative diseases | |
TREANOR et al. | Therapeutic Use of Neurotrophic | |
D DAWBARN et al. | Neurotrophins and neurodegenerative | |
Neet et al. | Selectivity of Cell Signaling in the Neuronal Response Based on NGF Mutations and Peptidomimetics in the Treatment of Alzheimers Disease |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 132251 Country of ref document: IL |
|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
ENP | Entry into the national phase |
Ref document number: 2286558 Country of ref document: CA Ref country code: CA Ref document number: 2286558 Kind code of ref document: A Format of ref document f/p: F |
|
ENP | Entry into the national phase |
Ref country code: JP Ref document number: 1998 547144 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1998918666 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 71546/98 Country of ref document: AU |
|
WWP | Wipo information: published in national office |
Ref document number: 1998918666 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1998918666 Country of ref document: EP |