WO1998042874B1 - Biomolecular processor - Google Patents
Biomolecular processorInfo
- Publication number
- WO1998042874B1 WO1998042874B1 PCT/US1998/006029 US9806029W WO9842874B1 WO 1998042874 B1 WO1998042874 B1 WO 1998042874B1 US 9806029 W US9806029 W US 9806029W WO 9842874 B1 WO9842874 B1 WO 9842874B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- test
- molecules
- carrying
- nucleic acid
- target
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract 13
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 9
- 108020004707 nucleic acids Proteins 0.000 claims abstract 9
- 238000010828 elution Methods 0.000 claims abstract 7
- 238000000034 method Methods 0.000 claims abstract 5
- 230000009089 cytolysis Effects 0.000 claims abstract 3
- 230000002934 lysing Effects 0.000 claims abstract 3
- 239000000523 sample Substances 0.000 claims 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- 239000012085 test solution Substances 0.000 claims 5
- 238000005259 measurement Methods 0.000 claims 4
- 239000003463 adsorbent Substances 0.000 claims 3
- 239000011616 biotin Substances 0.000 claims 3
- 238000006243 chemical reaction Methods 0.000 claims 3
- 238000009396 hybridization Methods 0.000 claims 3
- 239000000377 silicon dioxide Substances 0.000 claims 3
- 229920000160 (ribonucleotides)n+m Polymers 0.000 claims 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M buffer Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 2
- 238000001821 nucleic acid purification Methods 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 238000004166 bioassay Methods 0.000 claims 1
- 230000029918 bioluminescence Effects 0.000 claims 1
- 238000005415 bioluminescence Methods 0.000 claims 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 238000005382 thermal cycling Methods 0.000 claims 1
- 210000004369 Blood Anatomy 0.000 abstract 2
- 239000008280 blood Substances 0.000 abstract 2
- 210000002381 Plasma Anatomy 0.000 abstract 1
- 210000002700 Urine Anatomy 0.000 abstract 1
- 238000003149 assay kit Methods 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
Abstract
A process and apparatus for isolating and purifying nucleic acids and other target molecules directly from blood, plasma, urine, cell cultures and the like by totally automated means, without centrifugation, aspiration or vacuum; after mixing and heating a nucleic acid containing sample with lysis reagent in an environmentally isolated compartment, nucleic acids are absorbed onto a binding filter and eluted in a small volume using heated elution reagent; a preferred embodiment purifies nucleic acids and automatically detects target sequences from a sample of fresh blood. Another embodiment purifies target molecules from a multitude of samples held in microtiter plates; test kits for each embodiment include disposable isolation and detection devices and associated reagents.
Claims
-29-
v AMENDED CLAIMS
[received by the International Bureau on 3 December 1998 (03.12.98); original claim 17 cancelled; new claims 22-28 added; remaining claims unchanged (3 pages)]
18. A kit for carrying out the process according to Claim 1, for use with a machine constructed in accordance with Claims 2, 3, 4 or 7 comprising a disposable device constructed in accordance with Claims 2 , 3, 4 or 7 together with such lysis, wash and elution reagents as may be required to carry out the nucleic acid purification process.
19. A kit for carrying out the process according to Claim 1, for use with a machine constructed in accordance with Claim 5 comprising a multiplicity of disposable devices constructed in accordance with Claims 5 and 6, together with such lysis, wash and elution reagents as may be required to carry out the nucleic acid purification process.
20. An apparatus for mixing purified target molecules with components of an assay comprising
a) a first part which does not make physical contact with the starting sample material or molecules
-30-
22. An apparatus constructed according to Claim
21 in which at least one said disposable second part of Claim 2 is joined with at least one said disposable second part of Claim 20 in such manner that the two devices together comprise a single disposable device, said device having means for withdrawing a nucleic acid or other target molecule - containing sample inside itself and sealing such sample from the environment, with means for isolating and purifying the nucleic acid or other target molecule from the sample, with means for combining the purified molecules with components of at least one nucleic acid or other analytical test, with means for subjecting the said at least one test mixture to an exposure to at least one temperature optimal for carrying out the test or to multiple temperatures, with means for combining the said test mixture with at least one additional test reagent or with multiple reagents optimal for carrying out the test and for detecting the test results, and with means for measuring the results of a test without opening the apparatus or exposing any molecules of the test within the apparatus to the external environment.
23. An apparatus constructed according to Claim
22 in which the means for detecting test results is by measurements of light emission or light adsorption within a transparent portion of the apparatus, such as but not limited to measurements of an increase or a decrease in fluorescence, chemiluminescence, bioluminescence or light adsorption of the test mixture, of the test mixtures, such measurements being made without opening the device containing the test mixture or exposing any molecules of the test within the apparatus to the external environment.
24. The use of a test solution as an elution reagent for eluting purified target molecules from a solid matrix, said test solution being composed of reagents for determining the presence and/or amount of target nucleic acids or other molecules.
-31-
25. The use of a test solution, heated to between 20 to 100 degrees Celsius, but preferably to 95 degrees, composed of NTPs, buffers, primer DNA sequences and nucleic acid polymerases for carrying out the polymerase chain reaction, or RT-PCR, as an elution reagent for target RNA or DNA adsorbed onto silica or other adsorbents, or bound by hybridization or avidin-biotin or by another means, before carrying out thermal cycling.
26. The use of a test solution, heated to between 20 and 100 degrees Celsius, but preferably to an optimum annealing temperature for the NASBA reaction, composed of enzymes, buffers and primer sequences for carrying out the NASBA reaction, as an elution reagent for target RNA adsorbed onto silica or other adsorbents, or bound by hybridization or avidin-biotin or by another means, before carrying out the NASBA reaction.
27. The use of any target molecule test solution, heated to between 20 and 100 degrees Celsius, but preferably to an optimum test temperature, as an elution reagent for target molecules adsorbed onto silica or other adsorbents, or bound by hybridization or avidin-biotin or by another means, before carrying out the target molecule test.
28. An apparatus constructed according to claims 2, 20, and 22 in which the first part has means for receiving a multiplicity of samples held in sealed sample tubes and means for holding a multiplicity of second disposable parts constructed according to claim 22, with means for withdrawing samples from said sample tubes into said second parts to purify target molecules within said second parts, to combine purified target molecules with test reagents and to measure the test results of said multiple samples using multiple said second parts, without opening said second parts or exposing any molecules within to the external environment, said measurements being made sequentially or simultaneously.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU67790/98A AU6779098A (en) | 1997-03-24 | 1998-03-23 | Biomolecular processor |
| EP98913175A EP0972080B1 (en) | 1997-03-24 | 1998-03-23 | Biomolecular processor |
| DE69829466T DE69829466T2 (en) | 1997-03-24 | 1998-03-23 | BIOMOLECULAR PROCESSOR |
| AT98913175T ATE291637T1 (en) | 1997-03-24 | 1998-03-23 | BIOMOLECULAR PROCESSOR |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4123797P | 1997-03-24 | 1997-03-24 | |
| US60/041,237 | 1997-03-24 |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US09381603 A-371-Of-International | 1999-09-22 | ||
| US10/243,521 Continuation US20030027203A1 (en) | 1997-03-24 | 2002-09-12 | Biomolecular processor |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| WO1998042874A2 WO1998042874A2 (en) | 1998-10-01 |
| WO1998042874A3 WO1998042874A3 (en) | 1998-12-23 |
| WO1998042874B1 true WO1998042874B1 (en) | 1999-01-28 |
Family
ID=21915486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1998/006029 WO1998042874A2 (en) | 1997-03-24 | 1998-03-23 | Biomolecular processor |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0972080B1 (en) |
| AT (1) | ATE291637T1 (en) |
| AU (1) | AU6779098A (en) |
| DE (1) | DE69829466T2 (en) |
| WO (1) | WO1998042874A2 (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19746874A1 (en) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Isolation of nucleic acids |
| DE69938976D1 (en) * | 1999-03-11 | 2008-08-07 | Whatman Inc | Solid medium and methods for storage and rapid purification of nucleic acids |
| WO2001007451A2 (en) * | 1999-07-23 | 2001-02-01 | Tepnel Medical Limited | Isolation of biomolecules |
| US7229595B2 (en) * | 2001-06-15 | 2007-06-12 | Molecular Devices Corporation | Filtration column devices and methods of filtering therewith |
| US7749388B2 (en) | 2001-06-15 | 2010-07-06 | Life Technologies Corporation | Low volume filtration column devices and methods of filtering therewith |
| US7556733B2 (en) | 2001-06-15 | 2009-07-07 | Mds Analytical Technologies (Us) Inc. | Low volume filtration column devices and methods of filtering therewith |
| AU2002324289A1 (en) * | 2001-07-24 | 2003-02-17 | Societe D'amenagement Urbain Et Rural | Automated process for detecting pathogenic organisms in water |
| FR2827873B1 (en) * | 2001-07-24 | 2003-09-26 | Amenagement Urbain & Rural | AUTOMATED METHOD FOR DETECTING PATHOGENS IN WATER |
| EP1417306A2 (en) * | 2001-08-10 | 2004-05-12 | Xantos Biomedicine AG | High-throughput dna-isolation and transfection for analysing the function of genes or genetic products |
| DE20117746U1 (en) * | 2001-11-02 | 2002-04-25 | InViTek Gesellschaft für Biotechnik & Biodesign mbH, 13125 Berlin | Reaction rooms containing complex storage-stable reagent formulations and test kit for the detection and isolation of pathogenic microbial nucleic acids |
| DK3472351T3 (en) | 2016-06-15 | 2020-11-09 | Univ Muenchen Ludwig Maximilians | Single molecule detection or quantification using DNA nanotechnology |
| CN109696346B (en) * | 2017-10-20 | 2023-10-13 | 莱伯泰科(天津)科技有限公司 | Isotope pretreatment device |
| CN113030477B (en) * | 2019-12-25 | 2024-01-26 | 深圳迈瑞生物医疗电子股份有限公司 | Measurement method, measurement device for specific protein and storage medium |
| US20230294090A1 (en) * | 2020-06-19 | 2023-09-21 | National Institute For Viral Disease Control And Prevention, Chinese Center For Disease Control And | Sample processing and detection apparatus and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4775635A (en) * | 1985-04-12 | 1988-10-04 | E. I. Du Pont De Nemours And Company | Rapid assay processor |
| JPH04187077A (en) * | 1990-11-22 | 1992-07-03 | Shimadzu Corp | Apparatus for extraction and purification of nucleic acid |
| JP2832586B2 (en) * | 1995-08-04 | 1998-12-09 | 株式会社トミー精工 | DNA extraction and purification method |
| JP2000507927A (en) * | 1996-03-06 | 2000-06-27 | アクゾ・ノベル・エヌ・ベー | Automatic nucleic acid extraction from samples |
-
1998
- 1998-03-23 DE DE69829466T patent/DE69829466T2/en not_active Expired - Fee Related
- 1998-03-23 AT AT98913175T patent/ATE291637T1/en not_active IP Right Cessation
- 1998-03-23 WO PCT/US1998/006029 patent/WO1998042874A2/en active IP Right Grant
- 1998-03-23 AU AU67790/98A patent/AU6779098A/en not_active Abandoned
- 1998-03-23 EP EP98913175A patent/EP0972080B1/en not_active Expired - Lifetime
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