WO1998037209A3 - Expressionsvektor zur dauerhaften expression einer fremd-dna - Google Patents

Expressionsvektor zur dauerhaften expression einer fremd-dna Download PDF

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Publication number
WO1998037209A3
WO1998037209A3 PCT/DE1998/000539 DE9800539W WO9837209A3 WO 1998037209 A3 WO1998037209 A3 WO 1998037209A3 DE 9800539 W DE9800539 W DE 9800539W WO 9837209 A3 WO9837209 A3 WO 9837209A3
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WO
WIPO (PCT)
Prior art keywords
expression
expression vector
foreign dna
permanent
vector
Prior art date
Application number
PCT/DE1998/000539
Other languages
English (en)
French (fr)
Other versions
WO1998037209A2 (de
Inventor
Ingrid Grummt
Friedrich Grummt
Original Assignee
Deutsches Krebsforsch
Ingrid Grummt
Friedrich Grummt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforsch, Ingrid Grummt, Friedrich Grummt filed Critical Deutsches Krebsforsch
Priority to US09/367,927 priority Critical patent/US6300126B1/en
Priority to EP98914811A priority patent/EP0968296A2/de
Priority to JP53616498A priority patent/JP2001512320A/ja
Publication of WO1998037209A2 publication Critical patent/WO1998037209A2/de
Publication of WO1998037209A3 publication Critical patent/WO1998037209A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft einen Expressionsvektor mit einer Fremd-DNA, wobei am 3'-Ende der Fremd-DNA eine Sequenz vorliegt, die verhindert, daß die Replikation des Expressionsvektors in entgegengesetzter Richtung zu seiner Transkription verläuft. Ferner betrifft die Erfindung ein einen solchen Expressionsvektor enthaltendes Mittel sowie die Verwendung beider zur dauerhaften Expression einer Fremd-DNA in Zellen.
PCT/DE1998/000539 1997-02-24 1998-02-24 Expressionsvektor zur dauerhaften expression einer fremd-dna WO1998037209A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US09/367,927 US6300126B1 (en) 1997-02-24 1998-02-24 Expression vector for the permanent expression of foreign DNA
EP98914811A EP0968296A2 (de) 1997-02-24 1998-02-24 Expressionsvektor zur dauerhaften expression einer fremd-dna
JP53616498A JP2001512320A (ja) 1997-02-24 1998-02-24 外来ｄｎａの恒久的発現のための発現ベクター

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19707273.9 1997-02-24
DE19707273A DE19707273C1 (de) 1997-02-24 1997-02-24 Expressionsvektor zur dauerhaften Expression einer Fremd-DNA

Publications (2)

Publication Number Publication Date
WO1998037209A2 WO1998037209A2 (de) 1998-08-27
WO1998037209A3 true WO1998037209A3 (de) 1998-11-26

Family

ID=7821266

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/000539 WO1998037209A2 (de) 1997-02-24 1998-02-24 Expressionsvektor zur dauerhaften expression einer fremd-dna

Country Status (5)

Country Link
US (1) US6300126B1 (de)
EP (1) EP0968296A2 (de)
JP (1) JP2001512320A (de)
DE (1) DE19707273C1 (de)
WO (1) WO1998037209A2 (de)

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
GERBER J.K. ET AL.: "Termination of mammalian rDNA replication: polar arrest of replication fork movement by transcription termination factor TTF-I", CELL, vol. 90, 8 August 1997 (1997-08-08), pages 559 - 567, XP002079811 *
KUHN A. AND GRUMM I.: "3'end formation of mouse pre-rRNA involves both transcription termination and a specific processiing reaction", GENES AND DEVELOPMENT, vol. 3, 1989, pages 224 - 231, XP002079816 *
KUHN A. ET AL.: "The mouse ribosomal gene terminator consists of three functionally separable esquence elements", EMBO JOURNAL, vol. 7, no. 5, 1988, EYNSHAM, OXFORD GB, pages 1497 - 1502, XP002079814 *
LITTLE R.D ET AL.: "Initiation and termination of DNA replication in human rRNA genes", MOLECULAR AND CELLULAR BIOLOGY, vol. 13, no. 10, October 1993 (1993-10-01), WASHINGTON US, pages 6600 - 6613, XP002079812 *
LIU B. ET AL.: "A transcribing RNA Polymerase molecule survives DNA replication without aborting its growing RNA chain", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 91, October 1994 (1994-10-01), WASHINGTON US, pages 10660 - 10664, XP002079817 *
LIU. B AND ALBERTS B.: "Head-On collision between a DNA replication apparatus and RNA Polymerase transcription complex", SCIENCE, vol. 267, 24 February 1995 (1995-02-24), LANCASTER, PA US, pages 1131 - 1137, XP002079813 *
LOPEZ-ESTRANO C. ET AL.: "Co-localization of polar replication fork barriers and rRNA transcription terminators in mouse rDNA", JOURNAL OF MOLECULAR BIOLOGY, vol. 277, no. 2, 1998, pages 249 - 256, XP002079819 *
PFLEIDERER C. ET AL.: "An undecamer DNA sequence directs termination of human ribosomal gene transcription", NUCLEIC ACID RESEARCH, vol. 18, no. 16, 1990, OXFORD, GB, pages 4727 - 4736, XP002079815 *
VIGUERA E. ET AL.: "The ColE1 unidirectional origin acts as a polar replication fork pausing site", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 37, 13 September 1996 (1996-09-13), MD US, pages 22414 - 22421, XP002079818 *

Also Published As

Publication number Publication date
WO1998037209A2 (de) 1998-08-27
DE19707273C1 (de) 1998-09-24
EP0968296A2 (de) 2000-01-05
JP2001512320A (ja) 2001-08-21
US6300126B1 (en) 2001-10-09

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