WO1998031786A3 - Novel microorganisms - Google Patents

Novel microorganisms Download PDF

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Publication number
WO1998031786A3
WO1998031786A3 PCT/GB1998/000156 GB9800156W WO9831786A3 WO 1998031786 A3 WO1998031786 A3 WO 1998031786A3 GB 9800156 W GB9800156 W GB 9800156W WO 9831786 A3 WO9831786 A3 WO 9831786A3
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
novel microorganisms
novel
pathogenic
vaccines
Prior art date
Application number
PCT/GB1998/000156
Other languages
French (fr)
Other versions
WO1998031786A2 (en
Inventor
Jeremy Mark Wells
Page Richard William Falla Le
Christophe Francois Gu Gilbert
Original Assignee
Microbial Technics Limited
Jeremy Mark Wells
Page Richard William Falla Le
Christophe Francois Gu Gilbert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microbial Technics Limited, Jeremy Mark Wells, Page Richard William Falla Le, Christophe Francois Gu Gilbert filed Critical Microbial Technics Limited
Priority to JP53395898A priority Critical patent/JP2001510342A/en
Priority to EP98900912A priority patent/EP0973864A1/en
Priority to AU56719/98A priority patent/AU5671998A/en
Publication of WO1998031786A2 publication Critical patent/WO1998031786A2/en
Publication of WO1998031786A3 publication Critical patent/WO1998031786A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/09—Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus, streptococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Novel non-invasive or non-pathogenic gram-positive microorganisms are provided which are transformed or transfected with DNA coding for one or more enzymes responsible for the production of a polysaccharide immunogen from a pathogenic bacterium. Vaccines comprising such microorganisms and their use in therapy are also provided, as are suitable DNA constructs and vectors.
PCT/GB1998/000156 1997-01-17 1998-01-19 Novel microorganisms WO1998031786A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP53395898A JP2001510342A (en) 1997-01-17 1998-01-19 New microorganism
EP98900912A EP0973864A1 (en) 1997-01-17 1998-01-19 Novel microorganisms
AU56719/98A AU5671998A (en) 1997-01-17 1998-01-19 Novel microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9700939.3 1997-01-17
GBGB9700939.3A GB9700939D0 (en) 1997-01-17 1997-01-17 Therapy

Publications (2)

Publication Number Publication Date
WO1998031786A2 WO1998031786A2 (en) 1998-07-23
WO1998031786A3 true WO1998031786A3 (en) 1998-11-05

Family

ID=10806153

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/000156 WO1998031786A2 (en) 1997-01-17 1998-01-19 Novel microorganisms

Country Status (6)

Country Link
EP (1) EP0973864A1 (en)
JP (1) JP2001510342A (en)
AU (1) AU5671998A (en)
GB (1) GB9700939D0 (en)
WO (1) WO1998031786A2 (en)
ZA (1) ZA98387B (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1624064A3 (en) * 1998-07-27 2006-05-10 Microbial Technics Limited Nucleic acids and proteins from streptococcus pneumoniae
EP1144640A3 (en) * 1998-07-27 2001-11-28 Microbial Technics Limited Nucleic acids and proteins from streptococcus pneumoniae
US20030134407A1 (en) 1998-07-27 2003-07-17 Le Page Richard William Falla Nucleic acids and proteins from Streptococcus pneumoniae
US6936252B2 (en) 1998-07-27 2005-08-30 Microbial Technics Limited Streptococcus pneumoniae proteins and nucleic acid molecules
US7780961B2 (en) 2001-05-03 2010-08-24 Actogenix N.V. Self-containing Lactococcus strain
EP1513545B1 (en) 2002-06-19 2008-03-19 Actogenix N.V. Methods and means to promote gut absorption
ATE552843T1 (en) 2003-02-06 2012-04-15 Aduro Biotech LISTERIA WHOSE INVESTIGATION INTO NON-PHAGOCYTIC CELLS IS ATTENUATED, VACCINES CONTAINING THESE LISTERIA AND THEIR USES
US7695725B2 (en) 2003-02-06 2010-04-13 Aduro Biotech Modified free-living microbes, vaccine compositions and methods of use thereof
JP4839209B2 (en) 2003-02-06 2011-12-21 アンザ セラピューティクス，インコーポレイテッド Modified free-living microorganisms, vaccine compositions, and methods of use thereof
US20050220777A1 (en) * 2003-11-21 2005-10-06 Stefan Roos Selection of lactic acid bacteria enhancing the immune response against Streptococcus pneumoniae
US20080219960A1 (en) * 2004-12-16 2008-09-11 Masja Nathalie Nierop Groot Novel Efficient Production Process for Capsular Polysaccharides of Pathogenic Grampositive Bacteria by Heterologous Expression and Secretion of Complex Polysaccharides in Non-Pathogenic, Non-Invasive Gram-Positive Bacteria
EP1957100B1 (en) 2005-11-29 2016-07-13 Intrexon Actobiotics NV Induction of mucosal tolerance to antigens
EP2125010B1 (en) 2007-01-25 2014-06-04 Actogenix N.V. Treatment of immune disease by mucosal delivery of antigens using genetically modified lactobacillus
JP2012504116A (en) 2008-09-29 2012-02-16 アクトジェニックス・エヌブイ Reduction of microbial colonization in the mucosa

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497524A2 (en) * 1991-01-28 1992-08-05 Merck & Co. Inc. Polysaccharide antigens from streptococcus pneumoniae

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0497524A2 (en) * 1991-01-28 1992-08-05 Merck & Co. Inc. Polysaccharide antigens from streptococcus pneumoniae

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ARRECUBIETA, C. ET AL.: "Type 3-specific synthase of Streptococcus pneumoniae (Cap3B) directs Type 3 polysaccharide biosynthesis in Escherichia coli and in pneumococcal strains of different serotypes", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 184, no. 2, 1 August 1996 (1996-08-01), pages 449 - 455, XP002076310 *
DILLARD, J.P. ET AL.: "Characterization of the cassette containing genes for type 3 capsular polysaccharide biosynthesis in Streptococcus pneumoniae", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 181, no. 3, 1 March 1995 (1995-03-01), pages 973 - 983, XP002076313 *
GUIDOLIN, A. ET AL.: "Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F", INFECTION AND IMMUNITY, vol. 62, no. 12, December 1994 (1994-12-01), pages 5384 - 5396, XP002015452 *
NORTON, P.M. ET AL.: "Factors affecting the immunogenicity of tetanus toxin fragment C expressed in Lactococcus lactis", FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, vol. 14, no. 2-3, June 1996 (1996-06-01), pages 167 - 177, XP000615283 *
WELLS, J.M. ET AL.: "Lactic acid bacteria as vaccine delivery vehicles", ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, vol. 70, no. 2-4, October 1996 (1996-10-01), pages 317 - 330, XP002076311 *

Also Published As

Publication number Publication date
WO1998031786A2 (en) 1998-07-23
ZA98387B (en) 1999-07-16
AU5671998A (en) 1998-08-07
GB9700939D0 (en) 1997-03-05
EP0973864A1 (en) 2000-01-26
JP2001510342A (en) 2001-07-31

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