WO1998020146A3 - Stabilized transient gene expression - Google Patents
Stabilized transient gene expression Download PDFInfo
- Publication number
- WO1998020146A3 WO1998020146A3 PCT/US1997/019860 US9719860W WO9820146A3 WO 1998020146 A3 WO1998020146 A3 WO 1998020146A3 US 9719860 W US9719860 W US 9719860W WO 9820146 A3 WO9820146 A3 WO 9820146A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gene expression
- transient gene
- cells
- transient expression
- stabilized
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7135—Compounds containing heavy metals
- A61K31/714—Cobalamins, e.g. cyanocobalamin, i.e. vitamin B12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/131—Amines acyclic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
- A61K31/136—Amines having aromatic rings, e.g. ketamine, nortriptyline having the amino group directly attached to the aromatic ring, e.g. benzeneamine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
- A61K31/137—Arylalkylamines, e.g. amphetamine, epinephrine, salbutamol, ephedrine or methadone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/192—Carboxylic acids, e.g. valproic acid having aromatic groups, e.g. sulindac, 2-aryl-propionic acids, ethacrynic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
- A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/205—Amine addition salts of organic acids; Inner quaternary ammonium salts, e.g. betaine, carnitine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4164—1,3-Diazoles
- A61K31/4172—Imidazole-alkanecarboxylic acids, e.g. histidine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Abstract
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP10521611A JP2000509998A (en) | 1996-11-01 | 1997-10-31 | Stabilized transient gene expression |
EP97946432A EP0941356A2 (en) | 1996-11-01 | 1997-10-31 | Stabilized transient gene expression |
AU51601/98A AU732445B2 (en) | 1996-11-01 | 1997-10-31 | Stabilized transient gene expression |
CA002270137A CA2270137A1 (en) | 1996-11-01 | 1997-10-31 | Stabilized transient gene expression |
US09/093,449 US6146847A (en) | 1996-11-01 | 1998-06-08 | Stabilized transient gene expression |
US10/047,649 US20030073654A1 (en) | 1996-11-01 | 2002-01-16 | Stabilized transient gene expression |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3010996P | 1996-11-01 | 1996-11-01 | |
US60/030,109 | 1996-11-01 | ||
US83374797A | 1997-04-11 | 1997-04-11 | |
US08/833,747 | 1997-04-11 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US83374797A Continuation-In-Part | 1996-11-01 | 1997-04-11 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/093,449 Continuation-In-Part US6146847A (en) | 1996-11-01 | 1998-06-08 | Stabilized transient gene expression |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1998020146A2 WO1998020146A2 (en) | 1998-05-14 |
WO1998020146A3 true WO1998020146A3 (en) | 1998-08-20 |
Family
ID=26705677
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1997/019860 WO1998020146A2 (en) | 1996-11-01 | 1997-10-31 | Stabilized transient gene expression |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0941356A2 (en) |
JP (1) | JP2000509998A (en) |
CN (1) | CN1238807A (en) |
AU (1) | AU732445B2 (en) |
CA (1) | CA2270137A1 (en) |
WO (1) | WO1998020146A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533722B (en) * | 2010-12-24 | 2014-03-12 | 北京义翘神州生物技术有限公司 | Method for improving transient expression of recombinant proteins in mammalian cells through temperature jump |
CN106811483B (en) * | 2017-02-16 | 2020-04-07 | 甘肃农业大学 | Method for efficiently transfecting eukaryotic cells |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0486917A2 (en) * | 1990-11-17 | 1992-05-27 | BEHRINGWERKE Aktiengesellschaft | Antiviral activity from the rep gene of adenoassociated virus-2 |
WO1994016737A1 (en) * | 1993-01-26 | 1994-08-04 | Weiner David B | Compositions and methods for delivery of genetic material |
WO1995026718A1 (en) * | 1994-04-01 | 1995-10-12 | Apollon, Inc. | Compositions and methods for delivery of genetic material |
WO1996023898A1 (en) * | 1995-01-31 | 1996-08-08 | Novo Nordisk A/S | A method of detecting biologically active substances |
-
1997
- 1997-10-31 AU AU51601/98A patent/AU732445B2/en not_active Ceased
- 1997-10-31 CA CA002270137A patent/CA2270137A1/en not_active Abandoned
- 1997-10-31 WO PCT/US1997/019860 patent/WO1998020146A2/en not_active Application Discontinuation
- 1997-10-31 JP JP10521611A patent/JP2000509998A/en active Pending
- 1997-10-31 EP EP97946432A patent/EP0941356A2/en not_active Withdrawn
- 1997-10-31 CN CN97180186A patent/CN1238807A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0486917A2 (en) * | 1990-11-17 | 1992-05-27 | BEHRINGWERKE Aktiengesellschaft | Antiviral activity from the rep gene of adenoassociated virus-2 |
WO1994016737A1 (en) * | 1993-01-26 | 1994-08-04 | Weiner David B | Compositions and methods for delivery of genetic material |
WO1995026718A1 (en) * | 1994-04-01 | 1995-10-12 | Apollon, Inc. | Compositions and methods for delivery of genetic material |
WO1996023898A1 (en) * | 1995-01-31 | 1996-08-08 | Novo Nordisk A/S | A method of detecting biologically active substances |
Non-Patent Citations (12)
Title |
---|
ANDERSON J.W. & BRIDGES S.R.: "Short-chain fatty acid fermentation products of plant fiber affect glucose metabolism of isolated rat hepatocytes", PRO. SOC. EXP. BIOL. MED., vol. 177, 1984, pages 372 - 376, XP002067120 * |
BATTAGLIA C. ET AL: "Structural basis for beta 1 integrin-ediated cell adhesion to a large heparan sulfate proteoglycan from basement membranes", EUR. J. CELL. BIOL., vol. 61, no. 1, June 1993 (1993-06-01), pages 92 - 99, XP002065999 * |
BEIS I. & THEOPHILIDIS G.: "Phosphofructokinase in the plerocercoids of S. solidus", INT. J. PARASITOLOGY, vol. 12, no. 5, 1982, pages 389 - 394, XP002054819 * |
GOFFE AS & GOFFE RA: "Chemical stabilizingagents for transient DNA in cell culture".163rd AAAS National Meeting and Science Innovation Exposition, Poster A106. Seattle, WA, USA, February 13-18, 1997. * |
GOLDSTEIN S. ET AL.: "Enhanced transfection efficiency and improved cell survival after electroporation of G2/M-synchronized cells and treatment with sodium butyrate", NUCL . ACIDS RES., vol. 17, no. 10, 1989, pages 3959 - 3972, XP002054817 * |
GORMAN C.M. & HOWARD B.H.: "Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate", NUCL. ACIDS RES., vol. 11, no. 21, 1983, pages 7631 - 7648, XP002054816 * |
MISLICK K.A. & BALDSCHWIELER J.D.: "Evidence for the role of proteoglycans in cation-mediated gene transfer", PROC. NATL. ACAD. SCI. USA, vol. 93, October 1996 (1996-10-01), pages 12349 - 12354, XP002065997 * |
MORAND C. ET AL.: "Importance of the modulation of glycolysis in the control of lactate metabolism by fatty acids in isolated rat hepatocytes from fed rats", ARCH. BIOCHEM. BIOPHYS, vol. 309, no. 2, March 1994 (1994-03-01), pages 254 - 260, XP002067121 * |
NORDENBERG J ET AL.: "Inhibition of growth of human melanoma cells and enhancement of alkaline phosphatase", HAREFUAH, vol. 111, no. 1/2, - 1986, pages 59, 21 - 23, XP002065996 * |
PIEPKORN M.W. & CHAPMAN D.L.: "Glycosaminoglycans and the substrate attachment of murine myeloma, 3T3 and cutaneous fibrosarcoma cells.", LAB. INVEST., vol. 53, no. 1, July 1985 (1985-07-01), pages 22 - 29, XP002065998 * |
SATOH K. ET AL.: "Effect of drugs on gene expression in mammalian cells: a highly efficient procedure to test large numbers of samples", NUCL. ACIDS RES., vol. 21, no. 18, 1993, pages 4429 - 4430, XP002056202 * |
WARDEN D ET AL: "INFLUENCE OF DIETHYLAMINOETHYL-DEXTRAN ON UPTAKE AND DEGRADATION OFPOLYOMA VIRUS DEOXYRIBONUCLEIC ACID BY MOUSE EMBRYO CELLS", JOURNAL OF VIROLOGY, vol. 4, no. 4, 1 October 1969 (1969-10-01), pages 380 - 387, XP000569882 * |
Also Published As
Publication number | Publication date |
---|---|
WO1998020146A2 (en) | 1998-05-14 |
CN1238807A (en) | 1999-12-15 |
JP2000509998A (en) | 2000-08-08 |
CA2270137A1 (en) | 1998-05-14 |
AU732445B2 (en) | 2001-04-26 |
EP0941356A2 (en) | 1999-09-15 |
AU5160198A (en) | 1998-05-29 |
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