WO1998013076A1 - Reseau fibreux ensemence avec des cellules de culture et destines au remplacement de tissus - Google Patents

Reseau fibreux ensemence avec des cellules de culture et destines au remplacement de tissus Download PDF

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Publication number
WO1998013076A1
WO1998013076A1 PCT/US1997/015994 US9715994W WO9813076A1 WO 1998013076 A1 WO1998013076 A1 WO 1998013076A1 US 9715994 W US9715994 W US 9715994W WO 9813076 A1 WO9813076 A1 WO 9813076A1
Authority
WO
WIPO (PCT)
Prior art keywords
seeded
wound
membrane
tissue
lattice
Prior art date
Application number
PCT/US1997/015994
Other languages
English (en)
Inventor
Dennis P. Orgill
Charles E. Butler
Ioannis V. Yannas
Carolyn C. Compton
Original Assignee
Brigham And Women's Hospital
Massachusetts Institute Of Technology
Shriners Hospitals For Children
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brigham And Women's Hospital, Massachusetts Institute Of Technology, Shriners Hospitals For Children filed Critical Brigham And Women's Hospital
Priority to AU42645/97A priority Critical patent/AU4264597A/en
Publication of WO1998013076A1 publication Critical patent/WO1998013076A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Abstract

La présente invention se rapporte à une méthode de régénération de tissus chez un mammifère souffrant d'une brûlure ou d'une lésion. La méthode consiste à couvrir la lésion avec une membrane comprenant un réseau fibreux biodégradable et, en option, une barrière extérieure d'humidité extérieure; le réseau est ensemencé avec des cellules de culture obtenues à partir d'une culture ayant atteint la sous-confluence. Cette méthode permet d'infiltrer la matrice greffée par des cellules mésenchymateuses et par des vaisseaux sanguins, qui proviennent d'un tissu sous-jacent sain, pour former un nouveau derme, et de provoquer la prolifération des kératinocytes pour former un nouvel épiderme. La greffe qui en résulte se distingue par d'excellents taux de prise; elle a l'apparence d'un tissu normal, dont elle possède également la croissance, la maturation et la différentiation.
PCT/US1997/015994 1996-09-24 1997-09-10 Reseau fibreux ensemence avec des cellules de culture et destines au remplacement de tissus WO1998013076A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU42645/97A AU4264597A (en) 1996-09-24 1997-09-10 Cultured cell-seeded fibrous lattice for tissue replacement

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71781596A 1996-09-24 1996-09-24
US08/717,815 1996-09-24

Publications (1)

Publication Number Publication Date
WO1998013076A1 true WO1998013076A1 (fr) 1998-04-02

Family

ID=24883609

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/015994 WO1998013076A1 (fr) 1996-09-24 1997-09-10 Reseau fibreux ensemence avec des cellules de culture et destines au remplacement de tissus

Country Status (2)

Country Link
AU (1) AU4264597A (fr)
WO (1) WO1998013076A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004039248A2 (fr) * 2002-10-31 2004-05-13 The General Hospital Corporation Reparation ou remplacement de tissus ou d'organes
EP1419791A1 (fr) * 2001-07-30 2004-05-19 Japan Tissue Engineering Co., Ltd. Materiau de base de regeneration de tissu, materiau implantaire et procede de production correspondant

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983001384A1 (fr) * 1981-10-26 1983-04-28 Massachusetts Inst Technology Technique d'ensemencement cellulaire comprenant l'utilisation de treillis fibreux
US4963489A (en) * 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
WO1991013638A1 (fr) * 1990-03-05 1991-09-19 Smith & Nephew Plc Produits de culture cellulaire
WO1991016010A1 (fr) * 1990-04-24 1991-10-31 Mark Eisenberg Equivalents composites de peau vivante
WO1992010217A1 (fr) * 1990-12-05 1992-06-25 Vitaphore Wound Healing Inc. Pansements et procedes de fabrication
WO1993025660A1 (fr) * 1992-06-11 1993-12-23 Brigham And Women's Hospital Systeme et procede pour la transplantation de cellules
US5489304A (en) * 1994-04-19 1996-02-06 Brigham & Women's Hospital Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft
WO1996012510A1 (fr) * 1994-10-25 1996-05-02 Boehringer Mannheim Gmbh Biomateriau contenant des cellules epitheliales et son utilisation en tant que materiau de greffe
WO1997006750A1 (fr) * 1995-08-11 1997-02-27 The Regents Of The University Of California Keratinocytes humains portes par une membrane hydrophile et procedes d'utilisation pour la fermeture de plaies
WO1997006837A1 (fr) * 1995-08-16 1997-02-27 Integra Lifesciences Corporation Greffes de peau artificielle perforee
WO1997026023A1 (fr) * 1996-01-19 1997-07-24 Eth, Eidgenössische Technische Hochschule Zürich Pansements pour blessure et appareil associe

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458678A (en) * 1981-10-26 1984-07-10 Massachusetts Institute Of Technology Cell-seeding procedures involving fibrous lattices
WO1983001384A1 (fr) * 1981-10-26 1983-04-28 Massachusetts Inst Technology Technique d'ensemencement cellulaire comprenant l'utilisation de treillis fibreux
US4963489A (en) * 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
EP0681846A2 (fr) * 1990-03-05 1995-11-15 Smith & Nephew P.L.C. Produits de culture cellulaire
WO1991013638A1 (fr) * 1990-03-05 1991-09-19 Smith & Nephew Plc Produits de culture cellulaire
WO1991016010A1 (fr) * 1990-04-24 1991-10-31 Mark Eisenberg Equivalents composites de peau vivante
WO1992010217A1 (fr) * 1990-12-05 1992-06-25 Vitaphore Wound Healing Inc. Pansements et procedes de fabrication
WO1993025660A1 (fr) * 1992-06-11 1993-12-23 Brigham And Women's Hospital Systeme et procede pour la transplantation de cellules
US5489304A (en) * 1994-04-19 1996-02-06 Brigham & Women's Hospital Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft
WO1996012510A1 (fr) * 1994-10-25 1996-05-02 Boehringer Mannheim Gmbh Biomateriau contenant des cellules epitheliales et son utilisation en tant que materiau de greffe
WO1997006750A1 (fr) * 1995-08-11 1997-02-27 The Regents Of The University Of California Keratinocytes humains portes par une membrane hydrophile et procedes d'utilisation pour la fermeture de plaies
WO1997006837A1 (fr) * 1995-08-16 1997-02-27 Integra Lifesciences Corporation Greffes de peau artificielle perforee
WO1997026023A1 (fr) * 1996-01-19 1997-07-24 Eth, Eidgenössische Technische Hochschule Zürich Pansements pour blessure et appareil associe

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
I V YANNAS ET AL.: "WOUND TISSUE CAN UTILIZE A POLYMERIC TEMPLATE TO SYNTHESIZE A FUNCTIONAL EXTENSION OF SKIN", SCIENCE, vol. 215, January 1982 (1982-01-01), pages 174 - 176, XP002050504 *
S T BOYCE AND J F HANSBROUGH: "BIOLOGIC ATTACHMENT, GROWTH AND DIFFERENTIATION OF CULTURED HUMAN EPIDERMAL KERATINOCYTES ON A GRAFTABLE COLLAGEN AND CHONDROITIN-6-SULFATE SUBSTRATE", SURGERY, vol. 103, no. 4, April 1988 (1988-04-01), pages 421 - 431, XP002050503 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1419791A1 (fr) * 2001-07-30 2004-05-19 Japan Tissue Engineering Co., Ltd. Materiau de base de regeneration de tissu, materiau implantaire et procede de production correspondant
EP1419791A4 (fr) * 2001-07-30 2006-02-01 Japan Tissue Eng Co Ltd Materiau de base de regeneration de tissu, materiau implantaire et procede de production correspondant
WO2004039248A2 (fr) * 2002-10-31 2004-05-13 The General Hospital Corporation Reparation ou remplacement de tissus ou d'organes
WO2004039248A3 (fr) * 2002-10-31 2004-10-14 Gen Hospital Corp Reparation ou remplacement de tissus ou d'organes

Also Published As

Publication number Publication date
AU4264597A (en) 1998-04-17

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