WO1998007451A1 - Asepsie a l'air chaud - Google Patents

Asepsie a l'air chaud Download PDF

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Publication number
WO1998007451A1
WO1998007451A1 PCT/AU1997/000537 AU9700537W WO9807451A1 WO 1998007451 A1 WO1998007451 A1 WO 1998007451A1 AU 9700537 W AU9700537 W AU 9700537W WO 9807451 A1 WO9807451 A1 WO 9807451A1
Authority
WO
WIPO (PCT)
Prior art keywords
air
decontamination
cradle
sterilised
heating
Prior art date
Application number
PCT/AU1997/000537
Other languages
English (en)
Inventor
Robert Thomas Mullins
Andrew William Hinsch
Original Assignee
Forbio Limited
Shelbourne Investments Pty. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forbio Limited, Shelbourne Investments Pty. Ltd. filed Critical Forbio Limited
Priority to AU38415/97A priority Critical patent/AU3841597A/en
Publication of WO1998007451A1 publication Critical patent/WO1998007451A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/04—Heat
    • A61L2/06—Hot gas
    • A61L2/07—Steam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A61L2/0023—Heat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/04—Heat
    • A61L2/06—Hot gas
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/24—Apparatus using programmed or automatic operation

Definitions

  • THIS INVENTION is related to the decontamination and/or sterilisation of equipment. More particularly, the present invention is of use in the decontamination and/or sterilisation of equipment which comes into contact with biological tissue, especially plant tissue.
  • Microbial contamination is critical for success in the micro-propagation of plants by tissue culture.
  • Microbial contaminants can originate from the environment wherein the tissue culture is performed, from operators undertaking the tissue culture, or from the plants themselves.
  • the equipment used to cut and manipulate plants is a key vector for the spread of this microbial contamination between plants.
  • tweezers and scalpels used to cut and manipulate plants are sterilised between each tray of plants processed by the operator. Sterilisation is usually achieved by heat and/or chemicals.
  • Sterilisation is usually achieved by heat and/or chemicals.
  • tweezers and scalpels are dipped in a solution of ethanol and then heated using (a) small ovens known as "bacti-cinerators", (b) heat beads, (c) d e flame of a gas burner, or (d) high pressure steam in an autoclave.
  • the surfaces of the equipment used are exposed to temperatures of several hundred degrees centigrade for periods ranging from a few seconds to several minutes.
  • This control of contamination is more important for automated than manual tissue culture because of the larger number of plants processed through the automated procedures and the consequent increased potential to spread micro-organisms from one plant to another.
  • tissue culture include:
  • Decontamination procedures in other industries include the use of a "Hot Air Gun”.
  • such guns have been used for de-contaminating medical equipment such as syringes during their manufacture.
  • a decontamination device for an automated system of the type which includes an apparatus at least one surface of which requires decontamination and/or sterilisation, there is provided a decontamination device wherein said device is capable of decontaminating and/or sterilising said surface by the application of air at an elevated temperature to said surface.
  • said device comprises:
  • a body which houses an element for heating air to a required temperature to effect said decontamination and/or sterilisation
  • a cradle attached to said body, said cradle adapted to support a tool or other item to be sterilised;
  • an air nozzle attached to said body to direct hot said air to said cradle affixed to an end of said nozzle.
  • 1.5 litres of air per second are supplied by said device generating a zone of air at between 250° and 350°C.
  • said surface is one which comes into contact with biological tissue.
  • said biological tissue is plant tissue.
  • said device can be regulated to operate for only specified intervals during the processing of plants, typically after each container of plants has passed through the automated system.
  • the air is heated for 90 seconds before the surfaces to be decontaminated and/or sterilised are exposed to the heated air and the air flow continues for 120 seconds after the heating is switched off to cool said device before its re-use.
  • the device as herein described is particularly suited for incorporation into automated systems to decontaminate the tools used for cutting and handling plants, the systems being the subject of Australian patent nos. 591678 and 638868, the entire contents of which are incorporated herein by reference.
  • a method of rendering a surface aseptic by the application of air at an elevated temperature to said surface is provided.
  • said application of air to said surface is by use of a decontamination device as hereinbefore described.
  • FIG. 1 is a schematic illustration of a device according to the present invention adapted for incorporation into an automated plant tissue division machine.
  • FIG. 2 is a schematic illustration of the device of FIG 1 incorporated into an automated plant tissue division machine.
  • FIG. 3 is a graphical presentation of test results on microbial populations present after using the device and method of the present invention.
  • the device (1) comprises an air intake (2) leading to a body (3) which houses the element (not depicted) for heating the incoming air to the required temperature. Power is supplied to the element along an electrical cord (4).
  • a shaped air nozzle (5) is attached to the body (3) which directs the hot air to a U- shaped cradle (6) affixed to the end of the nozzle.
  • the U-shaped cradle (6) can support a tool or other item to be sterilised.
  • a multiple of devices (1) are affixed to an automated plant tissue division apparatus (7), each device (1) with a nozzle (5) and cradle (6) shaped and orientated as necessary to supply the required support to the tools.
  • 1.5 litres of air per second pass through the device to generate a zone of air at between 250° and 350°C.
  • the air and heating element are activated for 90 seconds before the surfaces of the cradle (6) are ready to be decontaminated.
  • the air flow continues for 120 seconds after the heating elements are switched off to allow the device (1) to cool.
  • the cutting surfaces are washed in baths of disinfectant cleaning solutions, typically 70% ethanol, to remove biological material.
  • disinfectant cleaning solutions typically 70% ethanol
  • the disinfectant solutions are usually agitated by stirring, by bubbling of air therethrough or by ultra- sonication.
  • a peat/sand filtrate which was used as a source of microbial contaminants, was prepared. Filamentous fungi, yeasts and bacterial organisms were present in this filtrate.
  • the cutting tool was first dipped into the filtrate and then immersed in an ultra- sonicated 70% ethanol bath for 30 seconds. Subsequently, the tool was exposed to hot air for varying periods (0 seconds, 30 seconds, 1 minute, 1.5 minutes and 2 minutes) after which it was allowed to cool for 15 seconds before being rubbed over and pushed into the surface of a single plate of potato dextrose agar (PDA)(200g potatoes, 20g glucose, 20g technical agar, 11 water). All plates were sealed with plastic film and incubated under near UV light at 25 °C for 14 days.
  • PDA potato dextrose agar
  • Treatment efficacies are expressed graphically in FIG 3 in terms of the average number of microbial colonies which were recovered per plate of PDA.
  • the device and method of the present invention thus offer a number of advantages for automated systems for plant propagation and micro-propagation, including:
  • the only inputs required can be a compressed air supply and electricity
  • the device is small and is easily built into the structure of existing automated systems.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

Dispositif conçu pour être utilisé dans un système automatisé du type comprenant un appareil dont au moins une surface nécessite d'être soit décontaminée, soit stérilisée, en particulier, l'équipement venant en contact avec un tissu biologique, en particulier, un tissu végétal. On effectue la décontamination ou la stérilisation de ladite surface en lui appliquant de l'air à température élevée. Ce dispositif (1) comprend une admission d'air (2) conduisant à un corps (3) logeant un élément servant à réchauffer l'air d'arrivée à la température nécessaire. Du courant électrique alimente l'élément par un cordon électrique (4). Un ajutage d'air (5) profilé est relié au corps (3) et dirige l'air chaud vers un support arqué (6) en forme de U fixé à l'extrémité de l'ajutage afin de supporter un outil ou un autre article à stériliser. On peut fixer une grande variété de dispositifs (1) à un appareil automatisé (7) de séparation de tissu végétal. En fonctionnement, 1,5 litres d'air à la seconde passent à travers le dispositif afin de générer une zone d'air dont la température est située entre 250 °C et 350 °C. L'air et l'élément de réchauffement sont activés pendant 90 secondes avant que les surfaces du support arqué (6) soient prêtes à être décontaminées. L'air continue à s'écouler pendant 120 secondes après l'arrêt des éléments de réchauffement, ce qui permet au dispositif (1) de refroidir. On peut éventuellement, avant le traitement thermique, laver la surface à décontaminer dans un bain de solution de nettoyage désinfectante, à 70° d'éthanol dans les cas typiques, de manière à supprimer tout matériau biologique.
PCT/AU1997/000537 1996-08-20 1997-08-20 Asepsie a l'air chaud WO1998007451A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU38415/97A AU3841597A (en) 1996-08-20 1997-08-20 Hot air asepsis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPO1762A AUPO176296A0 (en) 1996-08-20 1996-08-20 Hot air asepsis
AUPO1762 1996-08-20

Publications (1)

Publication Number Publication Date
WO1998007451A1 true WO1998007451A1 (fr) 1998-02-26

Family

ID=3796075

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU1997/000537 WO1998007451A1 (fr) 1996-08-20 1997-08-20 Asepsie a l'air chaud

Country Status (2)

Country Link
AU (1) AUPO176296A0 (fr)
WO (1) WO1998007451A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935604A (en) * 1988-09-29 1990-06-19 Dentronix, Inc. Method and apparatus for hot air sterilization of medical instruments
EP0427051A1 (fr) * 1989-11-07 1991-05-15 Tetra Laval Holdings & Finance SA Procédé de production d'un fluide gazeux contenant de l'hydrogène peroxyde
WO1996030058A1 (fr) * 1995-03-28 1996-10-03 Karl Schad Appareil de nettoyage et/ou de sterilisation d'instruments chirurgicaux
EP0758611A1 (fr) * 1995-08-11 1997-02-19 Societe Des Produits Nestle S.A. Procédé et appareil pour stériliser des surfaces

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935604A (en) * 1988-09-29 1990-06-19 Dentronix, Inc. Method and apparatus for hot air sterilization of medical instruments
EP0427051A1 (fr) * 1989-11-07 1991-05-15 Tetra Laval Holdings & Finance SA Procédé de production d'un fluide gazeux contenant de l'hydrogène peroxyde
WO1996030058A1 (fr) * 1995-03-28 1996-10-03 Karl Schad Appareil de nettoyage et/ou de sterilisation d'instruments chirurgicaux
EP0758611A1 (fr) * 1995-08-11 1997-02-19 Societe Des Produits Nestle S.A. Procédé et appareil pour stériliser des surfaces

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DERWENT ABSTRACT, Accession No. 92-380596/46, Class P34; & SU,A,1 704 788 (KIEV FOOD IND TECHN INST), 15 January 1992. *

Also Published As

Publication number Publication date
AUPO176296A0 (en) 1996-09-12

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