WO1997049431A2 - UTILISATION D'OLIGOSACCHARIDES POUR NEUTRALISER DES TOXINES DE $i(E. COLI) - Google Patents

UTILISATION D'OLIGOSACCHARIDES POUR NEUTRALISER DES TOXINES DE $i(E. COLI) Download PDF

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Publication number
WO1997049431A2
WO1997049431A2 PCT/CA1997/000436 CA9700436W WO9749431A2 WO 1997049431 A2 WO1997049431 A2 WO 1997049431A2 CA 9700436 W CA9700436 W CA 9700436W WO 9749431 A2 WO9749431 A2 WO 9749431A2
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WIPO (PCT)
Prior art keywords
αgal
pharmaceutical composition
infection
oligosaccharide
synsorb
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PCT/CA1997/000436
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English (en)
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WO1997049431A3 (fr
Inventor
David J. Rafter
Bradley P. Thompson
Peter N. Mclaine
Peter C. Rowe
Elaine Orrbine
Terrance P. Klassen
Glen D. Armstrong
Paul R. Goodyer
Andrew M. Mackenzie
George A. Wells
Hermy Lior
François AUCLAIR
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Synsorb Biotech, Inc.
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Priority claimed from US08/786,930 external-priority patent/US5849714A/en
Application filed by Synsorb Biotech, Inc. filed Critical Synsorb Biotech, Inc.
Priority to CA 2243118 priority Critical patent/CA2243118A1/fr
Priority to EP97926934A priority patent/EP0914162A2/fr
Priority to IL12764997A priority patent/IL127649A0/xx
Priority to AU31616/97A priority patent/AU733712B2/en
Publication of WO1997049431A2 publication Critical patent/WO1997049431A2/fr
Publication of WO1997049431A3 publication Critical patent/WO1997049431A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7016—Disaccharides, e.g. lactose, lactulose
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • This invention relates to the treatment of diarrhea caused by pathogenic E. coli infection. More specifically, this invention relates to methods for the neutralization of shiga-like toxins (SLT) associated with enteric E. coli infection which methods inhibit progression of this infection into hemolytic uremic syndrome (HUS).
  • SLT shiga-like toxins
  • HUS hemolytic uremic syndrome
  • Garegg, P. J. , et al. "A Synthesis of 8-Methoxycarbonyloct-l-yl O- ⁇ -D-Galactopyranosy l-( 1 -3)-0-0-D-Galactopyranosy l-( 1 -4)-2- Acetamido-2-Deoxy-/3-D-Glucopyranoside" , Carbohy. Res. , 136: 207-213 (1985).
  • Diarrhea caused by strains of pathogenic E. coli has been found to be associated with the production of a variety of enterotoxins.
  • Some pathogenic E. coli produce enterotoxins that are closely related to the shiga toxin associated with Shigella-cmsed dysentery.
  • the first member of the family of shiga-like toxins (SLT) to be isolated was cytotoxic for African Green Monkey (Vero) cells and was originally called verotoxin. Since its structural similarity to shiga toxin has been established by sequencing of the relevant genes, this toxin is now more commonly called shiga-like toxin I (SLTI) [5,6,7] .
  • SLT shiga-like toxins
  • SLTII SLT-specific immunodeficiency virus
  • Various types of SLTII have been described and have been assigned various designations depending on the strain of E. coli from which they are isolated and the host affected. Thus variants have been designated SLTII; vtx2ha; SLTIIvh; vtx2hb; SLTIIc; SLTIIvp and so forth.
  • All of the SLT's are multimeric proteins composed of an enzymatic (A) subunit and multiple (B) subunits.
  • the B oligomer is the binding portion of the toxin that allows it to bind to host cell receptors.
  • the B subunits of SLTI, SLTII and SLTIIvh recognize host cell globoseries glycolipid receptors containing at minimum the disaccharide subunit ⁇ Gal(l-4)0Gal at the non- reducing terminus; SLTII vp has been shown to bind to the receptors containing this subunit but not necessarily to the non-reducing end [2, 44-51].
  • the A subunit has an enzymatic activity (N-glycosidase) that depurinates 28S ribosomal RNA in mammalian cells. This enzymatic activity abolishes the ability of the toxin- infected cell to perform protein synthesis.
  • SLT The site for SLT action is endothelial cells found in the kidneys and mesenteric vasculature, and SLTs may cause damage that can result in renal failure and hemoglobin in the urine. SLTs are the causative agent in the hemolytic-uremic syndrome. SLTs may also be partially involved in the pathogenesis of hemorrhagic colitis (bloody diarrhea).
  • HUS hemolytic uremic syndrome
  • One reported method for the treatment of such infections is to orally administer a pharmaceutically inert affinity support comprising an ⁇ Gal(l ⁇ 4)j8Gal subunit to the infected patient [10].
  • This support passes into the intestinal tract of the patient whereupon the ⁇ Gal(l ⁇ 4)
  • the toxin bound to this solid support is eliminated from the body as part of the stool. This procedure is one of the first, if only, reported methods for removing such toxins from the body which, in turn, inhibits manifestation of the conditions associated with toxin accumulation.
  • This invention is directed to the surprising and unexpected discovery that the clinical incidence of HUS arising from enterohemorrhagic E. coli infection is significantly reduced by the time critical administration of a pharmaceutical composition comprising a pharmaceutically inert affinity support comprising an ⁇ Gal(l ⁇ 4)/JGal subunit which subunit binds the SLT.
  • a pharmaceutical composition comprising a pharmaceutically inert affinity support comprising an ⁇ Gal(l ⁇ 4)/JGal subunit which subunit binds the SLT.
  • this invention is directed to a method for inhibiting the development of hemolytic uremic syndrome in a patient arising from enterohemorrhagic E. coli infection mediated by shiga-like toxins which method comprises administering to said patient an effective amount of a pharmaceutical composition comprising a pharmaceutically inert affinity support comprising an ⁇ :Gal(l ⁇ 4)/JGal subunit which is bound to said support through a non-peptidyl linker arm, wherein said subunit binds SLT toxin and wherein this pharmaceutical composition is administered within about 3 days of presentation of the infection.
  • the pharmaceutical composition is administered to the patient prior to organ involvement other than involvement of the intestine.
  • the presentation of the infection is determined after the identification of at least one condition associated with an SLT mediated E. coli infection.
  • Such conditions include, for example, patients with diarrhea and one of the following: abdominal cramping, blood in the stool, rectal prolapse, detection of a verotoxin-producing E. coli in the patient's stool; ingestion of food suspected of containing a verotoxin-producing E. coli; or close contact with an individual known to have an SLT mediated infection.
  • the presentation of the infection is manifested by bloody diarrhea.
  • the initial clinical evaluation that the individual is afflicted with an SLT mediated E. coli infection is confirmed via diagnostic evaluation of the stool.
  • One diagnostic tool commercially available for detecting SLT mediated E. coli infection is sold by Meridian Diagnostic, Inc., Cincinnati, Ohio, USA 45244 under the name Premier EHEC.
  • the invention provides a method for inhibiting the development of hemolytic uremic syndrome in a patient presenting an enterohemorrhagic E. coli infection mediated by shiga-like toxin, which method comprises administering to the patient an effective amount of a pharmaceutical composition comprising a pharmaceutically inert affinity support comprising an oligosaccharide selected from the group consisting of ⁇ Gal(l ⁇ 4)/?Gal, ⁇ Gal(l ⁇ 4)j3Gal(l ⁇ 4)j3GlcNAc and ⁇ Gal(l ⁇ 4)0Gal(l ⁇ 4)/?Glc which oligosaccharide is bound to the support through a non-peptidyl linker arm, wherein this pharmaceutical composition is administered within about 3 days of presentation of the infection.
  • the pharmaceutical composition is administered to the patient prior to organ involvement other than the intestine.
  • Figure 1A and B demonstrate the toxicity of bacterial extracts obtained using polymyxin-B with respect to their ability to kill Vero cells in the presence and absence of various SYNSORBs.
  • Figure 2A and B demonstrate the toxicity of bacterial extracts obtained using lysozyme with respect to their ability to kill Vero cells in the presence and absence of various SYNSORBs.
  • Figure 3 A demonstrates that as little as 10 mg of P k trisaccharide
  • SYNSORB removes > 90% of SLT toxins from bacterial extracts.
  • Figure 3B demonstrates that the binding of the SLT toxins occurred within 5 minutes of mixing extracts with the P k SYNSORB.
  • Figure 4 demonstrates that difficulty in eluting the bound I 125 labelled SLTI from various SYNSORBs utilizing a variety of eluants.
  • Figure 5 demonstrates that > 90% SLTI, SLTII/IIc and SLTII activity was neutralized by co-incubation of Vero cells and SLT extracts for three days, with as little as 10 mg of P k trisaccharide SYNSORB. DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • this invention relates to methods for the neutralization of shiga-like toxins (SLT) associated with enteric E. coli infection which methods inhibit progression of this infection into hemolytic uremic syndrome (HUS).
  • SLT shiga-like toxins
  • HUS hemolytic uremic syndrome
  • shiga-like toxin or "SLT” or “verotoxin” refers to a group of toxins produced by enterohemorrhagic E. coli that resemble the Shigella- produced shiga toxins as is commonly understood in the art. These toxins comprise an enzymatically active A subunit and a multimeric receptor binding B subunit.
  • SLTs include SLTI and the various grouped toxins designated in the art as SLTII.
  • organ involvement refers to clinically defined organ involvement mediated by SLTs which correlates to the natural progression of the disease.
  • Organs other than the intestines include, by way of example, the kidney, heart, elements of the central nervous system ("CNS") (i.e. , brain, spinal cord, etc.), liver, and the like.
  • CNS central nervous system
  • Conventional blood chemistry tests can evaluate liver, heart, and kidney involvement whereas clinical symptoms including dementia, convulsions, disorientation, etc. are employed to determine
  • Hemolytic uremic syndrome is considered to be present if there is renal injury and either hemolysis or thrombocytopenia. Renal injury requires an elevation in the serum creatinine concentration ( > 50 ⁇ mol/L for those less than 5 years, or > 60 ⁇ mol/L for those 5-6 years) or a difference in the recorded creatinine values during the acute phase of the illness of more than 50% or at least 10 red blood cells per high power field on urine microscopy.
  • Hemolysis is judged to be present if the hemoglobin concentration is ⁇ 105g/L or if there are red blood cell fragments on the smear or if a red blood cell transfusion was administered before the hemoglobin dropped to ⁇ 105g/L.
  • Thrombocytopenia is defined as a platelet concentration of less than 150 X 10 9 /L.
  • biocompatible refers to chemical inertness with respect to animal or human tissues or body fluids. Biocompatible materials are non- sensitizing.
  • compatible linker arm refers to a moiety which serves to space the oligosaccharide structure from the biocompatible solid support and which is bifunctional wherein one functional group is capable of covalently binding to a reciprocal functional group of the support and the other functional group is capable of binding to a reciprocal functional group of the oligosaccharide structure.
  • Compatible linker arms preferred in the present invention are non-peptidyl linker arms. That is to say that the linker arms do not employ a peptide group to link the oligosaccharide structure to the solid support.
  • solid support refers to an inert, solid material to which the oligosaccharide sequences is bound via a compatible linker arm. Where use is in vivo, the solid support will be biocompatible.
  • the solid supports to which the oligosaccharide structures of the present invention are bound may be in the form of particles.
  • a large variety of biocompatible solid support materials are known in the art. Examples thereof are silica, synthetic silicates such as porous glass, biogenic silicates such as diatomaceous earth, silicate-containing minerals such as kaolinite, and synthetic polymers such as polystyrene, polypropylene, and polysaccharides.
  • Solid supports made of inorganic materials are preferred.
  • the solid supports have a particle size of from about 10 to 500 microns for in vivo use. In particular, particle sizes of 100 to 200 microns are preferred.
  • SYNSORB refers to synthetic 8-methoxycarbonyloctyl oligosaccharide structures covalently coupled to CHROMOSORB PTM (Manville Corp. , Denver, Colorado) [11], which is a derivatized silica particle.
  • Synthetic oligosaccharide sequences covalently attached to a biocompatible solid support e.g., CHROMOSORB PTM (SYNSORB) may be used to bind SLT toxins or verotoxins. These compositions are useful to prevent HUS and associated conditions. SYNSORB is particularly preferred for these compositions because it is non-toxic and resistant to mechanical and chemical decomposition. SYNSORBs have been found to pass unaffected through the rat gastrointestinal tract. They were found to be eliminated completely and rapidly (99% eliminated in 72 hours) following oral administration. Additionally, the high density of oligosaccharide moieties on SYNSORB is particularly useful for binding verotoxin.
  • Linking moieties that are used in indirect bonding are preferably organic bifunctional molecules of appropriate length (at least one carbon atom) which serve simply to distance the oligosaccharide structure from the surface of the solid support.
  • compositions of this invention are preferably represented by the formula:
  • OLIGOSACCHARIDE- Y-R n - SOLID SUPPORT
  • OLIGOSACCHARIDE comprises the ⁇ Gal(l ⁇ 4)/3Gal subunit and contains at least two 2 sugar units and preferably no more than 6 sugar units which oligosaccharide binds to the shiga-like toxin
  • Y is oxygen, sulfur or nitrogen
  • R is an aglycon linking arm of at least 1 carbon atom
  • SOLID SUPPORT is as defined above
  • n is greater than or equal to 1.
  • Preferred aglycons are from 1 to about 10 carbon atoms. Oligosaccharide sequences containing about 2 to 10 saccharide units may be used. Sequences with about 2 to 3 saccharide units are preferred.
  • n is such that the composition contains about 0.25 to 2.50 micromoles oligosaccharide per gram of composition.
  • linking arms are known in the art.
  • a linking arm comprising a para-nitrophenyl group (i.e. , -OC 6 H 4 pNO 2 ) has been disclosed [29].
  • the nitro group is reduced to an amino group which can be protected as N -trifluoroacetamido.
  • the trifluoroacetamido group is removed thereby unmasking the amino group.
  • linking arm containing sulfur has been disclosed [30]. Specifically, the linking arm is derived from a 2-bromoethyl group which, in a substitution reaction with thionucleophiles, has been shown to lead to linking arms possessing a variety of terminal functional groups such as -OCH 2 CH 2 SCH 2 CO 2 CH 3 and -OCH 2 CH 2 SC 6 H 4 -pNH 2 . These terminal functional groups permit reaction to complementary functional groups on the solid support, thereby forming a covalent linkage to the solid support. Such reactions are well known in the art.
  • a 6-trifluoroacetamido-hexyl linking arm (-O-(CH 2 ) 6 -NHCOCF 3 ) has been disclosed [31] in which the trifluoroacetamido protecting group can be removed, unmasking the primary amino group used for coupling.
  • linking arms include the 7- methoxycarbonyl-3,6,dioxaheptyl linking arm [32]
  • allyl linking arms can be derivatized in the presence of 2-aminoethanethiol [36] to provide for a linking arm -OCH ⁇ CH J SCH J CH J NH ⁇
  • Other suitable linking arms have also been disclosed [12-14, 16, 17].
  • the aglycon linking arm is a hydrophobic group and most preferably, the aglycon linking arm is a hydrophobic group selected from the group consisting of -(CH 2 ) 8 C(O)-, -(CH 2 ) 5 OCH 2 CH 2 CH 2 - and -(CH 2 ) 8 CH 2 O-.
  • Non-peptidyl linking arms are preferred for use as the compatible linking arms of the present invention.
  • glycopeptides contain several, often different, oligosaccharides linked to the same protein.
  • Glycopeptides are also difficult to obtain in large amounts and require expensive and tedious purification.
  • BSA or HSA conjugates is not desirable, for example, due to questionable stability in the gastrointestinal tract when given orally.
  • compositions useful in the conduct of the methods of the invention comprise the ⁇ Gal(l-4)/3Gal disaccharide subunit which subunit can be used alone or in conjunction with a higher oligosaccharide, e.g. , the cuGal(l-4)
  • 8Gal disaccharide subunit is preferably found at the non-reducing terminus of an oligosaccharide.
  • the oligosaccharide is coupled to a solid support or coupled directly, preferably through a linking arm such as that described by Lemieux et al. [11].
  • the di and trisaccharide units may also be coupled directly to pharmaceutically acceptable carriers or constitute a portion of an oligosaccharide coupled to such carriers.
  • the chemical synthesis of all or part of the oligosaccharide glycosides first involves formation of a glycosidic linkage on the anomeric carbon atom of the reducing sugar or monosaccharide.
  • an appropriately protected form of a naturally occurring or of a chemically modified saccharide structure (the glycosyl donor) is selectively modified at the anomeric center of the reducing unit so as to introduce a leaving group comprising halides, trichloroacetimidate, acetyl, thioglycoside, etc.
  • the donor is then reacted under catalytic conditions well known in the art with an aglycon or an appropriate form of a carbohydrate acceptor which possesses one free hydroxyl group at the position where the glycosidic linkage is to be established.
  • aglycon moieties are known in the art and can be attached with the proper configuration to the anomeric center of the reducing unit.
  • the saccharide glycoside can be used to effect coupling of additional saccharide unit(s) or chemically modified at selected positions or, after conventional deprotection, used in an enzymatic synthesis.
  • chemical coupling of a naturally occurring or chemically modified saccharide unit to the saccharide glycoside is accomplished by employing established chemistry well documented in the literature [12-28].
  • the oligosaccharide structure(s) is covalently bound or noncovalently
  • the covalent bonding may be via reaction between functional groups on the support and the compatible linker arm of the oligosaccharide structure.
  • the inert affinity supports comprising an ⁇ Gal(l ⁇ 4)/3GaI subunit which is bound to said support through a non-peptidyl linker arm used in the methods of this invention are made by methods known in the art [11 , 12] as follows. In each case the 8-methoxycarbonyloctyl glycoside of the respective hapten is activated and ligated to a silylaminated solid support, wherein the matrix is comprised of SiO 2 , followed by the acetylation of the remaining amine groups on the solid support.
  • These formulations are:
  • P,-di which contains at least 0.60 ⁇ mol/g ⁇ Gal(l-4)/3Gal disaccharide
  • P,-tri which contains at least 0.91 ⁇ mol/g ⁇ Gal(l-4)/3Gal(l-4) / 3GlcNAc trisaccharide
  • compositions comprising one or more oligosaccharide structures, which bind SLT toxin and/or verotoxin, attached to a solid support.
  • compositions When used for oral administration, which is preferred, these compositions may be formulated in a variety of ways. It will preferably be in liquid or semisolid form. Compositions including a liquid pharmaceutically inert carrier such as water may be considered for oral administration. Other pharmaceutically compatible liquids or semisolids, may also be used. The use of such liquids and semisolids is well known to those of skill in the art.
  • compositions which may be mixed with semisolid foods such as applesauce, ice cream or pudding may also be preferred.
  • Formulations such as SYNSORBs, which do not have a disagreeable taste or aftertaste are preferred.
  • a nasogastric tube may also be used to deliver the compositions directly into the stomach.
  • Solid compositions may also be used, and may optionally and conveniently be used in formulations containing a pharmaceutically inert carrier, including conventional solid carriers such as lactose, starch, dextrin or magnesium stearate, which are conveniently presented in tablet or capsule form.
  • a pharmaceutically inert carrier including conventional solid carriers such as lactose, starch, dextrin or magnesium stearate, which are conveniently presented in tablet or capsule form.
  • the SYNSORB itself may also be used without the addition of inert pharmaceutical carriers, particularly for use in capsule form.
  • a pharmaceutically inert carrier is employed the carrier is typically employed in the amount ranging from about 1 to about 99 percent based on the total weight of the composition and more preferably from about 75 to about 95 weight percent.
  • Doses are selected to provide neutralization and elimination of SLT toxin and/or elimination of E. coli found in the gut of the affected patient.
  • Preferred doses are from about 0.25 to 1.25 micromoles of oligosaccharide/kg body weight/day, more preferably about 0.5 to 1.0 micromoles of oligosaccharide/kg body weight/day.
  • SYNSORB compositions described above this means about 0.5 to 1.0 gram SYNSORB/kg body weight/day, which gives a concentration of SYNSORB in the gut of about 20 mg/ml.
  • Administration is expected to be 2 to 4 times daily, preferably for a period of one week.
  • the specific dose level and schedule of administration will, of course, vary for each individual depending on factors such as the particular oligosaccharide structure employed, the age and condition of the subject, the extent of the disease condition, all of which are well within the skill of the art.
  • oligosaccharide-containing compositions of the present invention during a period of up to seven days will be useful in treating SLT-associated diarrhea and associated conditions.
  • formulations may also be considered for other means of administration such as per rectum.
  • the usefulness of these formulations may depend on the particular composition used and the particular subject receiving the treatment.
  • These formulations may contain a liquid carrier that may be oily, aqueous, emulsified or contain certain solvents suitable to the mode of administration.
  • compositions may be formulated in unit dose form, or in multiple or subunit doses.
  • orally administered liquid compositions should preferably contain about 1 micromole oligosaccharide/ml .
  • SLT toxin may be neutralized by oligosaccharide sequences comprising the ⁇ Gal(l ⁇ 4)
  • oligosaccharide sequences covalently attached to solid supports via non- peptidyl compatible linker arms have been found to neutralize SLT toxin effectively.
  • SYNSORBs which bind and neutralize SLT toxin activity.
  • oligosaccharide sequences attached to solid supports useful in the present invention include those which bind SLT toxin.
  • the binding affinity of an oligosaccharide to SLT toxin is readily detectable by a simple in vitro test, as for example, set forth in Example 1 below.
  • oligosaccharide sequences attached to solid supports which bind SLT toxin means those compositions which reduce endpoint titers from cytotoxic activity in vero cell assays by at least 50% and preferably by at least 95% , using the assay set forth in the Examples section.
  • oligosaccharide sequences attached to solid supports useful in the present invention are those which can bind SLT toxin significantly better
  • the effect of the compositions of the invention in neutralizing SLTs can be measured by comparing activity of the SLT with and without treatment with the compositions. Activity of the SLTs can be assayed by taking advantage of the toxicity of these compounds to Vero cells. Vero cells (ATCC CCL81) can be obtained from the American Type Culture Collection, Rockville MD.
  • the initial clinical evaluation that the individual is afflicted with an SLT mediated E. coli infection is confirmed via diagnostic evaluation of the stool.
  • One diagnostic tool commercially available for detecting SLT mediated E. coli infection is sold by Meridian Diagnostic, Inc. , Cincinnati, Ohio, USA 45244 under the name Premier EHEC.
  • ASA acetylated silylaminated hydrophobic
  • LPS lipopolysaccharide
  • SDS sodium dodecyl sulfate
  • Examples 1-3 are from Armstrong, et al. [10] and are included herein to establish that oligosaccharides comprising a ⁇ Gal(l ⁇ 4)0Gal subunit possess similar in vitro properties.
  • Example 4 below illustrates in vivo results for the P k trisaccharide and demonstrates the affect of timing of administration of the composition in reducing the incidence of HUS.
  • Example 1 SYNSORB - Verotoxicity Neutralization Assays. E. coli strains 0157:H-(E32511), which produces SLTII/SLTIIc and 026:H11(H19) which produces SLTI only or stain C600(933W), which produces SLTII only, were grown overnight at 37 °C on tryptic soy broth (Difco, Detroit, MI) agar plates. Polymyxin and lysozyme extracts were prepared as described previously [1,2].
  • the first neutralization assay was designed to test the ability of SYNSORBs to absorb SLT activity from the E. coli extracts for 30 min. at room temperature in 1.5 mL microcentrifuge tubes (Fisher) with 2 to 50 mg SYNSORB on an end-over-end rotator. The tubes were then removed from the apparatus and after the SYNSORB had settled to the bottom (a few seconds), serial five-fold dilutions of the absorbed extracts were prepared in unsupplemented MEM. Twenty (20) ⁇ L of each dilution was added to the appropriate wells in 96 well microtiter plates containing Vero cells. Bacterial extracts to which no SYNSORB was added served as controls.
  • the percentage of neutralization was computed from the equation: 100- (100[CD 50 oligosaccharide SYNSORB-treated extracted + CD 50 acetylated silyl- aminated (ASA) SYNSORB-treated extract]).
  • the non-parametric Mann- Whitney test using the two-tailed statistic T was employed to compute the significance level of difference between groups of independent observations [4] .
  • the second neutralization assay was designed to test the ability of P k trisaccharide SYNSORB to protect Vero cells from SLT activity over 3 days at 37 °C. This assay involved incubating 180 ⁇ L of serial five-fold dilutions of polymyxin extracts in ethylene oxide-sterilized 1.5 mL microcentrifuge tubes each containing 2, 5 or 10 mg of P k trisaccharide
  • Example 2 Iodinated SLTI Binding Assay Purified SLTI was iodinated in 12 x 75 mm acid- washed glass culture tubes coated with 40 ⁇ g of Iodo Gen (Pierce Chemical Co. , Rockford, Illinois,
  • SLTI purified SLTI was incubated for 1 min. with 20 MBq I25 -I labeled sodium iodide in 100 ⁇ L PBS.
  • the reaction mixture was passed through a glass wool-plugged Pasteur pipette into 200 ⁇ L PBS containing a solution of cysteine (1 mg/mL) in PBS as described by Armstrong, G.D. et al. [52]
  • 200 ⁇ L of PBS containing 1 % BSA was added to the mixture and the iodinated SLTI was purified by passing the solution through a 1 cm x 30 cm Sephadex-G 25 gel filtration column with 0.1 % BSA in PBS.
  • the efficiency of the iodination reaction was determined by measuring the number of counts that were incorporated into trichloroacetic acid precipitated protein. Aliquots of the iodinated SLTI was stored at -90°C.
  • the assays were performed in PBS containing 0.15% BSA to reduce nonspecific binding. 2 mg of the SYNSORB were incubated for 30 min. on an end-over-end rotator with approximately 20,000 dpm of the iodinated SLTI prepared above (specific activity, 2.2 x 10 7 dpm/ ⁇ g, CD 50 in the Verocytotoxicity assay, 0.4 pg/mL), in 0.5 mL PBS/BSA). The SYNSORB was then washed with 3 x 1 mL portions of PBS/BSA to remove unbound counts. The derivatized SYNSORB were counted in an LKB Rackgamma model 1270 Gamma Counter. Similarly, ASA was also employed to determine the effect of using this material. The results are shown in Table 1.
  • the SLT bound to P k -tri SYNSORB could be partially released using 0.1 M acetic acid, 6 M guanidine HCI, or by heating in boiling water bath for 30 min. in 10% SDS. However, neither 0.5 M lactose, 0.5 M galactose, or 0.2 M EDTA could displace the bound SLTI ( Figure 4).
  • P k trisaccharide SYNSORB was incubated for the various times at 37 °C in 0.01 M HCI to simulate conditions in the stomach, then washed extensively in PBS to remove the HCI. The HCl-treated SYNSORB was then tested for SLTI and SLTII neutralizing activity in the Vero cytotoxicity assay as described hereinabove, and by Armstrong, G.D. et al. [53].
  • E. coli 026:H11 (SLTI) or 0157:H (SLTII) were grown overnight at 37 °C on Tryptic Soy Agar (TSA).
  • TSA Tryptic Soy Agar
  • the bacteria from 5 plates were harvested in 3.0 mL PBS containing 0.1 mg/mL Polymyxin B sulfate. The resulting suspension was then clarified by centrifugation.
  • incubation with HCI does not appreciably diminish neutralizing activity.
  • various SYNSORBs were incubated for 2 hours at 37 °C in buffer or in rat intestinal sacs. The incubated SYNSORBs were assayed for neutralizing activity against SLTI and SLTII, generally as described above. Briefly, SYNSORBs recovered from the rat intestines were sonicated for 30 to 60 seconds in a Branson Model B-220 Ultrasonic Cleaner to disrupt clumps of aggregated material . The sonicated SYNSORBs were then washed 4 times with 5 mL of double distilled, deionized H 2 O and dried under vacuum.
  • Control SYNSORBs were treated in a similar manner.
  • the polymyxin-extract described above was diluted to 8 mL with PBS.
  • Five mg of SYNSORB was added to 0.9 mL portions of the diluted polymyxin extract. These were then incubated at room temperature for 1 hour on an end-over-end rotator.
  • the resulting supernatant solutions were analyzed for SLTI or SLTII activity. Percent neutralization was calculated relative to the CD 50 s of polymyxin extracts incubated with the ASA control SYNSORB. These results are shown in Tables 3-6.
  • Table 3 shows the results of neutralization of SLTI activity by SYNSORB incubated in buffer
  • Table 4 shows neutralization of SLTI activity by SYNSORB incubated in intestinal sacs
  • Table 5 shows neutralization of SLTII activity by SYNSORB incubated in buffer
  • Table 6 shows neutralization of SLTII activity by SYNSORB incubated in rat intestinal sacs.
  • a Average of triplicate determinations ⁇ standard deviation of the mean.
  • b Control SYNSORB containing only the acetylated silylaminated (ASA) hydrophobic 8-methoxycarbonyloctyl linkage arm.
  • ASA acetylated silylaminated
  • a complete blood count, peripheral blood smear, serum urea and creatinine urinalysis and serum for anti-0157 antibodies was obtained from all subjects prior to enrollment. Serum samples from enrollment and day 60 after enrollment were tested for anti-0157 LPS antibodies using a passive hemagglutination assay. An anti-0157 LPS titer of > 1 :500 was considered consistent with recent infection.
  • E. coli by standard biochemical tests, and isolates were serogrouped by agglutination using 0157 antisera. Stool samples from those with negative routine cultures were forwarded to the National Laboratory for Enteric Pathogens at the Center for Disease Control in Ottawa, Canada. These samples were evaluated for the presence of other verotoxin-producing E. coli.
  • Eligible subjects were stratified on the basis of age and center and then randomized. Patients received either SYNSORB-Pk mixed in baby food or an equal volume of ground corn meal placebo with similar taste and texture. The primary outcome of interest was the proportion of patients with evidence of HUS at day seven of treatment.
  • the study drug was administered twice daily for seven days.
  • the dose of SYNSORB-Pk was approximately 500 mg/kg/day.
  • the placebo was ground corn meal, selected because it had a mildly gritty texture similar to SYNSORB- Pk and like SYNSORB-Pk had no taste.
  • both SYNSORB-Pk and the corn meal placebo were mixed in a commercially available baby food (fruit). If a dose was vomited within 30 minutes, it was readministered.
  • Subjects continued to receive the study drug for seven days if their stool culture results identified E. coli 0157 or if no bacterial pathogen was identified. If the stool culture identified an alternate cause for symptoms, such as Salmonella, Shigella, Campy lobacter or Yersinia species, the subject stopped taking the medication.
  • the primary outcome measure for the trial was the proportion of subjects with hemolytic uremic syndrome by the time of laboratory re- evaluation 8 -10 days after enrollment. Hemolytic uremic syndrome was considered to be present if there was renal injury and either hemolysis or thrombocytopenia. Renal injury required an elevation in the serum creatinine concentration ( > 50 ⁇ mol/L for those less than 5 years, or > 60 ⁇ mol/L for those 5-6 years) or a difference in the recorded creatinine values during the acute phase of the illness of more than 50% or at least 10 red blood cells per high power field on urine microscopy.
  • Hemolysis was judged present if the hemoglobin concentration was ⁇ 105g/L or if there were red blood cell fragments on the smear or if a red blood cell transfusion was administered before the hemoglobin dropped to ⁇ 105g/L. All peripheral blood smears were forwarded to the central study laboratory at the Children's Hospital of Eastern Ontario in Ottawa, where they were graded by laboratory technicians blinded to the treatment status of the subject.
  • Thrombocytopenia was defined as a platelet concentration of less than
  • results of this evaluation were analyzed to determine the effect of this protocol on those patients with a verified SLT mediated pathogenic E. coli infection and who were compliant with the treatment regimen.
  • the analysis of these results were divided into two groups, i.e. , those treated within three days of first presenting symptoms of E. coli infection and those treated after three days of first presenting symptoms of E. coli infection.
  • Tables 7 and 8 below set forth the results of this evaluation.

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Abstract

L'invention repose sur une découverte surprenante et inattendue selon laquelle l'incidence clinique du syndrome hémolytique et urémique (SHU) consécutive à une infection entérohémorrhagique par E. Coli peut se trouver notablement réduite par l'administration en temps voulu d'une préparation pharmaceutique comprenant un support à affinité pharmaceutique inerte comportant une sous unité αGal(1>4)βGal se fixant aux toxines de type Shiga (SLT). On a en particulier découvert que l'incidence clinique du SHU consécutive à une infection entérohémorrhagique par le E. Coli se trouvait réduite lorsque ladite préparation pharmaceutique était administrée dans les trois jours suivant l'apparition de l'infection. Par contre, l'administration de ladite préparation pharmaceutique après cette période ou lorsque d'autres organes que l'intestin sont touchés par l'infection en réduit sensiblement la capacité à réduire l'incidence du SHU.
PCT/CA1997/000436 1996-06-21 1997-06-20 UTILISATION D'OLIGOSACCHARIDES POUR NEUTRALISER DES TOXINES DE $i(E. COLI) WO1997049431A2 (fr)

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CA 2243118 CA2243118A1 (fr) 1996-06-21 1997-06-20 Utilisation d'oligosaccharides pour neutraliser des toxines de e. coli
EP97926934A EP0914162A2 (fr) 1996-06-21 1997-06-20 UTILISATION D'OLIGOSACCHARIDES POUR NEUTRALISER DES TOXINES DE $i(E. COLI)
IL12764997A IL127649A0 (en) 1996-06-21 1997-06-20 Use of oligosaccharides for neutralising e. coli toxins
AU31616/97A AU733712B2 (en) 1996-06-21 1997-06-20 Use of oligosaccharides for neutralising E. coli toxins

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999042136A1 (fr) * 1998-02-23 1999-08-26 Synsorb Biotech, Inc. Composes et procedes pour le traitement de la dysenterie bacterienne, au moyen d'antibiotiques et de compositions a base d'oligosaccharide fixant les toxines
WO2000051644A1 (fr) * 1999-03-04 2000-09-08 The Governors Of The University Of Alberta Traitement de la diarrhee provoquee par escherichia coli enteropathogenes

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EP0305968A2 (fr) * 1987-08-31 1989-03-08 Yeda Research And Development Company Limited Compositions contre des maladies protozoiques
WO1991016072A1 (fr) * 1990-04-16 1991-10-31 President And Fellows Of Harvard College Conjugues hydroxyapatites-antigenes et procedes permettant de produire une reponse immune poly-ig
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WO1995029701A1 (fr) * 1994-05-03 1995-11-09 Yeda Research And Development Co., Ltd. Vaccins d'immunisation a immuniser par voie orale contre des agents d'infection

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EP0101027A1 (fr) * 1982-08-18 1984-02-22 Herbert Prof. Dr. Rupprecht Composés organiques liés à un support de silice, procédé de leur préparation et leur emploi
WO1986004064A1 (fr) * 1984-12-27 1986-07-17 Biocarb Ab Compose et composition utilises pour la therapie et le diagnostic; utilisation de ce compose et de cette composition dans le traitement therapeutique et l'isolement de shigatoxine
EP0305968A2 (fr) * 1987-08-31 1989-03-08 Yeda Research And Development Company Limited Compositions contre des maladies protozoiques
WO1991016072A1 (fr) * 1990-04-16 1991-10-31 President And Fellows Of Harvard College Conjugues hydroxyapatites-antigenes et procedes permettant de produire une reponse immune poly-ig
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ARMSTRONG G.D. ET AL: "A phase I study of chemically synthesized verotoxin (shiga-like toxin) Pk- trisaccharide receptors attached to Chromosorb for preventing hemolytic- uremic syndrome" JOURNAL OF INFECTIOUS DISEASES, 1995, VOL. 171, NO. 4, PAGE(S) 1042-1045, XP002049420 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999042136A1 (fr) * 1998-02-23 1999-08-26 Synsorb Biotech, Inc. Composes et procedes pour le traitement de la dysenterie bacterienne, au moyen d'antibiotiques et de compositions a base d'oligosaccharide fixant les toxines
US6224891B1 (en) 1998-02-23 2001-05-01 Synsorb Biotech, Inc. Compounds and methods for the treatment of bacterial dysentery using antibiotics and toxin binding oligosaccharide compositions
AU738081B2 (en) * 1998-02-23 2001-09-06 Synsorb Biotech Inc. Compounds and methods for the treatment of bacterial dysentery using antibiotics and toxin binding oligosaccharide compositions
WO2000051644A1 (fr) * 1999-03-04 2000-09-08 The Governors Of The University Of Alberta Traitement de la diarrhee provoquee par escherichia coli enteropathogenes
US6291435B1 (en) 1999-03-04 2001-09-18 The Governs Of The University Of Alberta Treatment of diarrhea caused by enteropathogenic Escherichia coli

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WO1997049431A3 (fr) 1998-03-12
AU3161697A (en) 1998-01-14

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