WO1997046626A1 - Inhibition of bacterial growth - Google Patents

Inhibition of bacterial growth Download PDF

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Publication number
WO1997046626A1
WO1997046626A1 PCT/GB1996/001374 GB9601374W WO9746626A1 WO 1997046626 A1 WO1997046626 A1 WO 1997046626A1 GB 9601374 W GB9601374 W GB 9601374W WO 9746626 A1 WO9746626 A1 WO 9746626A1
Authority
WO
WIPO (PCT)
Prior art keywords
coating composition
biocide
powder
powder coating
substrate
Prior art date
Application number
PCT/GB1996/001374
Other languages
French (fr)
Inventor
Rainer Clover
Harold Brodie
Original Assignee
Biocote Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25680715&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1997046626(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to DE1996614004 priority Critical patent/DE69614004T2/en
Priority to GB9825646A priority patent/GB2328691B/en
Priority to CA 2258256 priority patent/CA2258256C/en
Priority to AU60100/96A priority patent/AU734162B2/en
Priority to AT96917571T priority patent/ATE203263T1/en
Priority to JP50029498A priority patent/JP2000511554A/en
Priority to DK96917571T priority patent/DK0904323T3/en
Application filed by Biocote Limited filed Critical Biocote Limited
Priority to PT96917571T priority patent/PT904323E/en
Priority to ES96917571T priority patent/ES2160248T3/en
Priority to EP19960917571 priority patent/EP0904323B1/en
Priority to BR9612636A priority patent/BR9612636A/en
Priority to PCT/GB1996/001374 priority patent/WO1997046626A1/en
Publication of WO1997046626A1 publication Critical patent/WO1997046626A1/en
Priority to US09/201,287 priority patent/US5980620A/en
Priority to FI982617A priority patent/FI982617A/en
Priority to US09/412,066 priority patent/US6129782A/en
Priority to GR20010401789T priority patent/GR3036913T3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/03—Powdery paints
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/30—Derivatives containing the group >N—CO—N aryl or >N—CS—N—aryl
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/03—Powdery paints
    • C09D5/033—Powdery paints characterised by the additives
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/14—Paints containing biocides, e.g. fungicides, insecticides or pesticides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/31504—Composite [nonstructural laminate]
    • Y10T428/31678—Of metal
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/31504—Composite [nonstructural laminate]
    • Y10T428/31678—Of metal
    • Y10T428/31692—Next to addition polymer from unsaturated monomers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/31504—Composite [nonstructural laminate]
    • Y10T428/31678—Of metal
    • Y10T428/31692—Next to addition polymer from unsaturated monomers
    • Y10T428/31696—Including polyene monomers [e.g., butadiene, etc.]

Definitions

  • This invention relates to the inhibition of bacterial growth and particularly to the inhibition of bacterial growth on a substrate.
  • the invention provides a method of inhibiting the growth of bacteria on a substrate, the method comprising forming a substantially dry powder coating composition containing a biocide, applying the powder coating composition to form a coating on the substrate, the biocide being capable of retaining effective biocidal activity in the coating.
  • composition is applied to the substrate by spraying by electrostatic or tribocharge means.
  • the invention provides a coating composition for use in the method of the immediately preceding paragraph, the composition containing 0.1 to 20% by weight of a biocide, the powder being substantially dry and being adapted for application to a substrate by electrostatic or tribocharged spraying.
  • the biocide is present in an amount of from 0.1 to 10% by weight.
  • the invention also provides a coated article made by the method of and/or comprising a coating according to the present invention.
  • the invention provides a powder mixture comprising a biocide powder and a powder or powders to form the matrix of the coating composition.
  • biocide will conveniently have the same physical characteristics as the matrix powder for ease of application.
  • the biocide may be incorporated in the powder to form the matrix of the coating. Whichever method is used, it is desirable that the biocide be homogeneously mixed throughout the powder composition. In practice, this may for example be achieved during manufacture of the powder composition.
  • the final powder therefore, in one preferred embodiment, has particulates each of which contains biocide, matrix powder and any other included ingredients. As each particulate contains the biocide it is homogeneously distributed throughout the coating composition and so cannot separate out.
  • such a powder may be made by adding the biocide at the initial mixing stage of the constituents which are to be converted into the desired matrix powder.
  • the precursors of the desired powder e.g.
  • the resin base and its hardener, together with any other additives, are mixed, heated and extruded to sheet form, the sheet is granulated and then ground to the desired powder size. Excellent mixing of the constituents is thereby achieved and all the constituents in the desired proportions are present in each of the individual particles of the final powder.
  • the coating composition preferably includes pigments and the powder to form the matrix of the coating composition, therefore, includes in this embodiment suitable pigmentation.
  • the coating composition is preferably formed as a dry mixture of the powder constituents.
  • the biocide may be selected to be active against Gram positive and/or Gram negative bacteria, algae, filamentous fungi or yeasts and may be a general purpose biocide that is active against more than one such category.
  • biocides may be suitably employed in the invention and the average skilled man of the art will readily be able to determine by routine experimentation whether the biocidal activity of any particular biocide will be sufficiently retained for his particular needs in the coating.
  • the necessary criteria are that the biocide can be provided in a suitable powder form and that it can survive the coating process.
  • biocides Particularly suitable classes of biocides have been found to include:
  • methyl ureas e.g. 3.- (3,4 - dichlorophenyl) - 1,1 - dimethyl urea
  • the matrix of the coating composition may be provided by any suitable material that can be manufactured in powder form, gives the desired properties, e.g. durability, to the applied coating and bonds satisfactorily to the substrate surface.
  • the article to which the coating is applied is preferably metal, although the invention is not limited thereto and includes, for example, domestic appliances (so-called white and brown goods), work surfaces for domestic and industrial use, architectural and other engineering products, such as hand rails, door handles and plates; garden furniture; and the like.
  • Typical substrates may be. for example, ferrous metals, zinc-coated steels, aluminium and the like. The article may thus be used in any environment where hygiene is advantageous.
  • powdered coating compositions of the invention may be applied, as indicated above, by electrostatic spraying which may be manual or automatic, or by tribocharged spraying.
  • Particularly suitable materials to fo ⁇ n the matrix of the coating composition include epoxy, polyesters and epoxy-polyesters and are available in powder form, pre- pigmented and to provide a variety of desired surface finishes - gloss, matt and textured.
  • the invention is not so limited and is also apphcable to the use of other polymeric powder materials, e.g. thermoplastic materials, e.g. nylon, acrylics, polyethylene, polypropylene and eva.
  • the matrix powders may have a specific gravity of from 1.2 to 1.9 and a particle size of which 100% is less than 100 microns and 40 to 60% is greater than 34 microns. They may have stoving temperatures, for example, of at least 120°, e.g. 140° to 210°C depending, of course, as the physical characteristics of the specific material used.
  • the biocide when used as a separate powder, is preferably chosen to have a siinilar particle size distribution to that of the matrix powder.
  • the amount of biocide incorporated in the powder composition will vary depending on the intended end use and the strength and nature of the particular biocide. Thus suitable amounts can readily be determined from the known mic values of the particular biocide. For example, up to 20% by weight of biocide based on the total powder composition may be used. Typically useful proportions are from 0.1 to 10% by weight of the total powder composition, especially from 2 to 6% by weight.
  • ingredients may be included in the powder composition, for example fluidity agents, dispersants, preservatives.
  • the powder mixture incorporating the biocide powder has good shelf life depending on the particular biocide used but, alternatively, may be mixed shortly prior to use.
  • the substrate must be thoroughly clean before application of the powder mixture and cleaning, e.g. by shot-blasting and/or chemical means may be carried out by conventional means.
  • Pigmented powdered coating compositions were made by mixing the following powder materials in the proportions shown
  • BIOCIDE was a 2,4,4 ' - tricholoro - 2 ' hydroxy diphenylether supplied by CIBA GEIGY as IRGASAN DP 300.
  • the matrix powder was a proprietory polyester powder. The powdered mixture was sprayed electro staticaUy onto one metal surface which had previously been degreased shot-blasted to provide several sample shdes coated on one surface only.
  • a powdered coating composition was made by mixing the following powder materials in the proportions shown.
  • the BIOCIDE was as in Example 1.
  • the matrix powder was a polyester supplied by H B Fuller Coatings Limited, Birmingham, England under the identification OMEGA PC P6/035.
  • the powder mixture was sprayed on to microscope glass shdes of about 25mm width at a temperature of 200°C for five minutes.
  • Bacterial lawn plates of eight different bacteria were prepared as follows.
  • the coated shdes were placed, coated face upwards in Petri dishes containing Agar nutrient as for Example 1.
  • a thin film of the nutrient covered the test shdes.
  • Each bacteria was applied in a line across a dish and continuing over the test slide. As before, a set of three samples were tested in each instance, i.e. in each Run.
  • DOI Degree of Inhibition
  • ZOI Zone of Inhibition
  • Example 2 was repeated but using the six organisms hsted in Table 3 below and using biocide concentrations of from 0 to 20% by weight. The Zones of Inhibition were again measured and are hsted in Table 3. TABLE 3

Abstract

The invention provides a means of inhibiting bacterial growth particularly on a coated substrate. It comprises a method of forming a substantially dry powder coating composition containing a biocide, applying the powder coating composition to form a coating on the substrate, the biocide being capable of retaining effective biocidal activity in the coating.

Description

INHIBITION OF BACTERIAL GROWTH
This invention relates to the inhibition of bacterial growth and particularly to the inhibition of bacterial growth on a substrate.
It is an object of the invention to provide a method of inhibiting bacterial growth when a coating composition is applied to a substrate.
It is also an object of the invention to provide a coating composition for use in the method of the invention.
In one aspect the invention provides a method of inhibiting the growth of bacteria on a substrate, the method comprising forming a substantially dry powder coating composition containing a biocide, applying the powder coating composition to form a coating on the substrate, the biocide being capable of retaining effective biocidal activity in the coating.
Preferably the composition is applied to the substrate by spraying by electrostatic or tribocharge means.
In another aspect the invention provides a coating composition for use in the method of the immediately preceding paragraph, the composition containing 0.1 to 20% by weight of a biocide, the powder being substantially dry and being adapted for application to a substrate by electrostatic or tribocharged spraying. Preferably the biocide is present in an amount of from 0.1 to 10% by weight. The invention also provides a coated article made by the method of and/or comprising a coating according to the present invention.
In one embodiment the invention provides a powder mixture comprising a biocide powder and a powder or powders to form the matrix of the coating composition.
In this embodiment the biocide will conveniently have the same physical characteristics as the matrix powder for ease of application.
Alternatively, the biocide may be incorporated in the powder to form the matrix of the coating. Whichever method is used, it is desirable that the biocide be homogeneously mixed throughout the powder composition. In practice, this may for example be achieved during manufacture of the powder composition. The final powder, therefore, in one preferred embodiment, has particulates each of which contains biocide, matrix powder and any other included ingredients. As each particulate contains the biocide it is homogeneously distributed throughout the coating composition and so cannot separate out. Thus, for example, such a powder may be made by adding the biocide at the initial mixing stage of the constituents which are to be converted into the desired matrix powder. In a typical powder manufacturing process, the precursors of the desired powder, e.g. the resin base and its hardener, together with any other additives, are mixed, heated and extruded to sheet form, the sheet is granulated and then ground to the desired powder size. Excellent mixing of the constituents is thereby achieved and all the constituents in the desired proportions are present in each of the individual particles of the final powder.
The coating composition preferably includes pigments and the powder to form the matrix of the coating composition, therefore, includes in this embodiment suitable pigmentation. The coating composition is preferably formed as a dry mixture of the powder constituents.
The biocide may be selected to be active against Gram positive and/or Gram negative bacteria, algae, filamentous fungi or yeasts and may be a general purpose biocide that is active against more than one such category.
Many biocides may be suitably employed in the invention and the average skilled man of the art will readily be able to determine by routine experimentation whether the biocidal activity of any particular biocide will be sufficiently retained for his particular needs in the coating. The necessary criteria are that the biocide can be provided in a suitable powder form and that it can survive the coating process.
Particularly suitable classes of biocides have been found to include:
trichloro hydroxy diphenyl ethers, e.g. 2,4,4 * - trichloro - 2^ - hydroxy diphenyl ether,
methyl ureas, e.g. 3.- (3,4 - dichlorophenyl) - 1,1 - dimethyl urea,
imidazolcarbamates.
The matrix of the coating composition may be provided by any suitable material that can be manufactured in powder form, gives the desired properties, e.g. durability, to the applied coating and bonds satisfactorily to the substrate surface.
The article to which the coating is applied is preferably metal, although the invention is not limited thereto and includes, for example, domestic appliances (so-called white and brown goods), work surfaces for domestic and industrial use, architectural and other engineering products, such as hand rails, door handles and plates; garden furniture; and the like. Typical substrates may be. for example, ferrous metals, zinc-coated steels, aluminium and the like. The article may thus be used in any environment where hygiene is advantageous.
The powdered coating compositions of the invention may be applied, as indicated above, by electrostatic spraying which may be manual or automatic, or by tribocharged spraying.
Particularly suitable materials to foπn the matrix of the coating composition include epoxy, polyesters and epoxy-polyesters and are available in powder form, pre- pigmented and to provide a variety of desired surface finishes - gloss, matt and textured. However, the invention is not so limited and is also apphcable to the use of other polymeric powder materials, e.g. thermoplastic materials, e.g. nylon, acrylics, polyethylene, polypropylene and eva.
Typically the matrix powders may have a specific gravity of from 1.2 to 1.9 and a particle size of which 100% is less than 100 microns and 40 to 60% is greater than 34 microns. They may have stoving temperatures, for example, of at least 120°, e.g. 140° to 210°C depending, of course, as the physical characteristics of the specific material used.
The biocide, when used as a separate powder, is preferably chosen to have a siinilar particle size distribution to that of the matrix powder.
The amount of biocide incorporated in the powder composition will vary depending on the intended end use and the strength and nature of the particular biocide. Thus suitable amounts can readily be determined from the known mic values of the particular biocide. For example, up to 20% by weight of biocide based on the total powder composition may be used. Typically useful proportions are from 0.1 to 10% by weight of the total powder composition, especially from 2 to 6% by weight.
Other ingredients may be included in the powder composition, for example fluidity agents, dispersants, preservatives.
The powder mixture incorporating the biocide powder has good shelf life depending on the particular biocide used but, alternatively, may be mixed shortly prior to use.
As is well appreciated in the coating art, the substrate must be thoroughly clean before application of the powder mixture and cleaning, e.g. by shot-blasting and/or chemical means may be carried out by conventional means.
Specific embodiments of the invention are further described in the following Examples:
Example 1
Pigmented powdered coating compositions were made by mixing the following powder materials in the proportions shown
Parts by weight
BIOCIDE - 0 to 6
Matrix powder - 100 to 94
The BIOCIDE was a 2,4,4 ' - tricholoro - 2 ' hydroxy diphenylether supplied by CIBA GEIGY as IRGASAN DP 300. The matrix powder was a proprietory polyester powder. The powdered mixture was sprayed electro staticaUy onto one metal surface which had previously been degreased shot-blasted to provide several sample shdes coated on one surface only.
Bacterial lawn plates of five different bacteria, as listed in Table 1 below, in Tryptone Soya Agar were prepared and a sample slide was placed, coated side down, in the centre of each plate. The plates were incubated at 37°C for 24-48 hours and observed for zones of inhibition.
The results are listed in Table 1 below, being based on 3 replicates per slide:
TABLE 1
Organism Percentage of Biocide
O 2 4 6
E.Coli — +— +— + —
P. aeruginosa — +— +— +—
S.typhimurium — — — —
S.aureus +— +— +» +—
S.faecalis — — +— —
+-- indicates inhibition no inhibition Example 2
A powdered coating composition was made by mixing the following powder materials in the proportions shown.
Parts by weight
BIOCIDE 6
Matrix powder 94
The BIOCIDE was as in Example 1.
The matrix powder was a polyester supplied by H B Fuller Coatings Limited, Birmingham, England under the identification OMEGA PC P6/035.
The powder mixture was sprayed on to microscope glass shdes of about 25mm width at a temperature of 200°C for five minutes.
Bacterial lawn plates of eight different bacteria, as listed in Table 2 below, were prepared as follows. The coated shdes were placed, coated face upwards in Petri dishes containing Agar nutrient as for Example 1. A thin film of the nutrient covered the test shdes. Each bacteria was applied in a line across a dish and continuing over the test slide. As before, a set of three samples were tested in each instance, i.e. in each Run.
In Table 2 are listed the Degree of Inhibition (DOI) and the Zone of Inhibition (ZOI) in mm. The DOI indicates the amount of the width of each line of bacteria that disappeared and the DOI indicates the amount of the length of the line of organisms across and on either side of its slide that disappeared. Run l Run 2
Organism DOI ZOI(mm) DOI ZOI(mm)
Staphylococcus Complete 55 Complete 55 aureas (Oxford)
Staphylococcus Complete 50 Complete 45 aureas (Resistant)
Bnterococcus 50% 25 50% 25 faecalis
Escherichia-coli Complete 30 Complete 30
Salmonella Complete 30 Complete 30 typhimurium
Pseudomonas NIL NIL aeruginosa
Corynebacterium 50% 25 50% 25 diptheria
(non-toxic)
Bacillus Complete 35 Complete 35 subtilis
Example 3
Example 2 was repeated but using the six organisms hsted in Table 3 below and using biocide concentrations of from 0 to 20% by weight. The Zones of Inhibition were again measured and are hsted in Table 3. TABLE 3
Organism Biocide concentration (%w/w)
0 6 10 15 20
E.coli 0 31 43.7 57.3 49.3
Strep faecalis 0 25 25 25 26.7
S. aureas (Oxford) 42 52.3 76.7 100 100
S aureas (Resistant) 42.3 53.7 100 100 100
P. aeruginosa 0 0 0 0 0
B. subtilis 0 26 32 41.6 43
Salmonella typhimurium 0 25.3 32 39 43
It will be appreciated that the invention is not limited to the specific embodiments described. For example, another suitable technique for applying the coating to a metal substrate is the so-called plastics coating technique in which the substrate is preheated and dipped into a bed of the powder. Such a technique also forms part of this invention.

Claims

1. A method of inhibiting the growth of bacteria on a substrate, the method comprising forming a substantially dry powder coating composition containing a biocide, applying the powder coating composition to form a coating on the
substrate, the biocide being capable of retaining effective biocidal activity in the coating.
2. A method according to Claim 1 , in which the composition is applied to the substrate by spraying by electrostatic or tribocharged means.
3. A method according to Claim 1 or 2, wherein the biocide is a powder and is mixed with a powder coating composition.
4. A method according to Claim 1 or 2. in which the biocide is mixed with the precursors of the powder or powders which are to foπn the matrix of the powder coating composition.
5. A method according to any preceding Claim, in which pigmentation is mixed into the powder coating composition.
6. A method according to any preceding Claim, wherein the coating is applied to the substrate using a stoving temperature of 140° to 210°C.
7. A method according to any preceding Claim, wherein the coating composition is applied to a metal substrate.
8. A method according to any preceding Claim, wherein the biocide is
incorporated in the powder coating composition in an amount of from 0.1 to 20% by weight.
9. A method according to Claim 8, wherein the biocide is incorporated in the powder coating composition in an amount of from 2 to 6% by weight.
10. For use in the method of any preceding Claim, a powder coating composition containing 0.1 to 20% by weight of a biocide, the powder being substantially dry and being adapted for apphcation to a substrate by electrostatic or tribocharged spraying or by dipping the preheated substrate.
1 1. A powder coating composition according to Claim 10, wherein the biocide is present in an amount of from 2 to 6% by weight.
12. A powder coating composition according to Claim 10 or 11, wherein the
biocide and the matrix of the coating composition are powders of substantially the same physical characteristics.
13. A powder coating composition according to Claim 10, 11 or 12, wherein the biocide is a trichloro hydroxy diphenyl ether.
14. A powder coating composition according to Claim 10, 1 1 or 12, wherein the biocide is 2,4,4] - trichloro - 21 - hydroxy diphenyl ether.
15. A powder coating composition according to Claim 10, 11 or 12, wherein the biocide is a methyl urea.
16. A powder coating composition to claim 15, wherein the biocide is 3 - (3,4 - dichlorophenyl) - 1 , 1 - dimethyl urea.
17. A powder coating composition according to Claim 10, 1 1 or 12 wherein the biocide is an imidazolcarbamate.
18. A powder coating composition according to any one of Claims 10 to 17, which comprises a polyester or epoxy polyester powder.
19. A powder coating composition according to any one of Claims 10 to 18, whjch comprises a thermoplastic powder, e.g. of nylon
20. A powder coating composition according to any one of Claims 10 to 19, which comprises a matrix powder of specific gravity from 1.2 to 1.9 and a particle size less than 100 microns.
21. A powder coating composition according to any one of Claims 10 to 20, in which the particulates of the powder each contain the biocide.
22. An article comprising a substrate having a coating apphed by the method of any one of Claims 1 to 9 or as defined in any one of Claims 10 to 21.
PCT/GB1996/001374 1996-06-05 1996-06-05 Inhibition of bacterial growth WO1997046626A1 (en)

Priority Applications (16)

Application Number Priority Date Filing Date Title
PT96917571T PT904323E (en) 1996-06-05 1996-06-05 INACTIVATION OF BACTERIAL GROWTH
ES96917571T ES2160248T3 (en) 1996-06-05 1996-06-05 BACTERIAL GROWTH INHIBITION.
GB9825646A GB2328691B (en) 1996-06-05 1996-06-05 Inhibition of microbial growth
EP19960917571 EP0904323B1 (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth
AT96917571T ATE203263T1 (en) 1996-06-05 1996-06-05 INHIBITION OF BACTERIAL GROWTH
JP50029498A JP2000511554A (en) 1996-06-05 1996-06-05 Bacterial growth inhibition
DK96917571T DK0904323T3 (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth
DE1996614004 DE69614004T2 (en) 1996-06-05 1996-06-05 INHIBITION OF BACTERIAL GROWTH
PCT/GB1996/001374 WO1997046626A1 (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth
CA 2258256 CA2258256C (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth
AU60100/96A AU734162B2 (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth
BR9612636A BR9612636A (en) 1996-06-05 1996-06-05 Bacterial growth inhibition
US09/201,287 US5980620A (en) 1996-06-05 1998-11-30 Inhibition of bacterial growth
FI982617A FI982617A (en) 1996-06-05 1998-12-04 Inhibition of bacterial growth
US09/412,066 US6129782A (en) 1996-06-05 1999-10-04 Inhibition of bacterial growth
GR20010401789T GR3036913T3 (en) 1996-06-05 2001-10-17 Inhibition of bacterial growth

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/GB1996/001374 WO1997046626A1 (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth
CA 2258256 CA2258256C (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/201,287 Continuation US5980620A (en) 1996-06-05 1998-11-30 Inhibition of bacterial growth

Publications (1)

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WO1997046626A1 true WO1997046626A1 (en) 1997-12-11

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PCT/GB1996/001374 WO1997046626A1 (en) 1996-06-05 1996-06-05 Inhibition of bacterial growth

Country Status (15)

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US (2) US5980620A (en)
EP (1) EP0904323B1 (en)
JP (1) JP2000511554A (en)
AT (1) ATE203263T1 (en)
AU (1) AU734162B2 (en)
BR (1) BR9612636A (en)
CA (1) CA2258256C (en)
DE (1) DE69614004T2 (en)
DK (1) DK0904323T3 (en)
ES (1) ES2160248T3 (en)
FI (1) FI982617A (en)
GB (1) GB2328691B (en)
GR (1) GR3036913T3 (en)
PT (1) PT904323E (en)
WO (1) WO1997046626A1 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999018162A1 (en) * 1997-10-03 1999-04-15 E.I. Du Pont De Nemours And Company, Inc. Biocidal powder coating composition, its preparation and use
EP1353761A1 (en) * 2000-11-15 2003-10-22 SSW Holding Company, Inc. Coating with anti-microbial agent for refrigerator shelving
GB2442440A (en) * 2006-10-04 2008-04-09 Philip Reed Anti-microbial cover for door furniture
US7357949B2 (en) 2001-12-21 2008-04-15 Agion Technologies Inc. Encapsulated inorganic antimicrobial additive for controlled release
EP1925201A1 (en) * 2006-11-09 2008-05-28 Werner Zapletal Method for antimicrobial treatment of powder
US8063116B2 (en) 2006-08-25 2011-11-22 Sciessent Llc Antimicrobial powder coatings and method
US8518449B2 (en) 2006-08-25 2013-08-27 Sciessent Llc Polymer particle coating method
US9861102B2 (en) 2016-05-26 2018-01-09 Markesbery Blue Pearl LLC Methods for disinfection
US11425911B2 (en) 2017-05-25 2022-08-30 Markesbery Blue Pearl LLC Method for disinfection of items and spaces

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT904323E (en) * 1996-06-05 2002-01-30 Biocote Ltd INACTIVATION OF BACTERIAL GROWTH
US6168800B1 (en) * 1998-08-20 2001-01-02 Medwrap Corporation Antimcrobial multi-layer island dressing
WO2001056778A1 (en) * 2000-02-04 2001-08-09 Certainteed Corporation Semipermeable coating for building materials
US6972145B1 (en) * 2000-02-04 2005-12-06 Certainteed Corporation Semipermeable coating for building materials
ATE328479T1 (en) * 2000-09-21 2006-06-15 Ciba Sc Holding Ag MIXTURES OF PHENOLIC AND INORGANIC MATERIALS THAT EXHIBIT ANTIMICROBIAL ACTIVITY
JP2002277816A (en) * 2001-03-21 2002-09-25 Minolta Co Ltd Image display device
US20030118658A1 (en) * 2001-12-21 2003-06-26 Trogolo Jeffrey A. High aspect ratio encapsulated inorganic antimicrobial additive for controlled release
US7135436B2 (en) * 2003-05-05 2006-11-14 J.F. Daley International, Ltd. Solid algicide, preparation and usage in recirculating water
US20050170001A1 (en) * 2003-12-05 2005-08-04 Adrien Lapeyre Polyamide-based powder and its use for obtaining an antibacterial coating
US20050159503A1 (en) * 2004-01-20 2005-07-21 Kim Young J. Antimicrobial-containing coating powder and method
US8600045B2 (en) * 2004-04-05 2013-12-03 Language Line Services, Inc. Configuration for antimicrobial multi-handest telephone system
US7901705B2 (en) * 2006-02-28 2011-03-08 Tyco Healthcare Group Lp Antimicrobial releasing polymers
WO2007100881A2 (en) * 2006-02-28 2007-09-07 Tyco Healthcare Group Lp Antimicrobial medical devices
WO2007109735A2 (en) * 2006-03-22 2007-09-27 Mississippi State University A nonleachable composition and method to use
US8772197B2 (en) * 2007-08-17 2014-07-08 Massachusetts Institute Of Technology Compositions for chemical and biological defense
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DE102013005479A1 (en) 2013-03-28 2014-10-02 Institut für Kunststofftechnologie und -recycling e.V. Process for the preparation of powder coating compositions with antimicrobial activity
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GB201320784D0 (en) 2013-11-25 2014-01-08 Xeros Ltd Improved cleaning Apparatus and method
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US9476010B2 (en) 2014-10-21 2016-10-25 Eastman Kodak Company Using imprinted multi-layer biocidal particle structure

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817762A (en) * 1973-04-18 1974-06-18 Du Pont Mildewcide for paint
US3988294A (en) * 1974-03-11 1976-10-26 R. T. Vanderbilt Company, Inc. Surface coating compositions containing antimicrobic ureas
FR2409250A1 (en) * 1977-11-21 1979-06-15 Ciba Geigy Ag NEW 3-HYDROXYDIPHENYLETHERS, THEIR PREPARATION PROCESS AND THEIR USE AS MICROBICIDES
JPH0625561A (en) * 1992-07-10 1994-02-01 Toagosei Chem Ind Co Ltd Antimicrobial powder coating composition
JPH0657032A (en) * 1992-08-06 1994-03-01 Toagosei Chem Ind Co Ltd Antimicrobial nylon resin composition
JPH0860036A (en) * 1994-08-19 1996-03-05 Nippon Ester Co Ltd Antibacterial antifungal polyester resin composition for powder coating

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817761A (en) * 1973-04-18 1974-06-18 Du Pont Mildewcide for paint
JPS59143635A (en) * 1983-02-07 1984-08-17 井上　鉄彦 Pasting coating for preventing mold using powdered body paint and manufacture thereof
JPS6025561A (en) * 1983-07-20 1985-02-08 Mitsubishi Heavy Ind Ltd Electrostatic desalting apparatus
JPS6057032A (en) * 1983-09-07 1985-04-02 Toyota Motor Corp Rubber vibration insulator
US4663364A (en) * 1984-09-05 1987-05-05 Kao Corporation Biocidal fine powder, its manufacturing method and a suspension for agricultural use containing the above powder
US5238749A (en) * 1986-03-27 1993-08-24 Clinitex Corporation Antimicrobial coating process and product
GB8621094D0 (en) * 1986-09-01 1986-10-08 Ici Plc Loading of polymer additives
GB8927375D0 (en) * 1989-12-04 1990-01-31 Swan Thomas & Co Ltd Powder coatings
FR2657085A1 (en) * 1990-01-16 1991-07-19 Toa Gosei Chem Ind PROCESS FOR STORING LOW TEMPERATURE CURING PULVERULENT COATINGS AND LOW TEMPERATURE CURING PULVERULENT COATING COMPOSITIONS HAVING EXCELLENT STORAGE STABILITY.
US5179414A (en) * 1991-01-22 1993-01-12 Compag Computer Corporation Apparatus for developing an image on a photoconductive surface
PT904323E (en) * 1996-06-05 2002-01-30 Biocote Ltd INACTIVATION OF BACTERIAL GROWTH

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817762A (en) * 1973-04-18 1974-06-18 Du Pont Mildewcide for paint
US3988294A (en) * 1974-03-11 1976-10-26 R. T. Vanderbilt Company, Inc. Surface coating compositions containing antimicrobic ureas
FR2409250A1 (en) * 1977-11-21 1979-06-15 Ciba Geigy Ag NEW 3-HYDROXYDIPHENYLETHERS, THEIR PREPARATION PROCESS AND THEIR USE AS MICROBICIDES
JPH0625561A (en) * 1992-07-10 1994-02-01 Toagosei Chem Ind Co Ltd Antimicrobial powder coating composition
JPH0657032A (en) * 1992-08-06 1994-03-01 Toagosei Chem Ind Co Ltd Antimicrobial nylon resin composition
JPH0860036A (en) * 1994-08-19 1996-03-05 Nippon Ester Co Ltd Antibacterial antifungal polyester resin composition for powder coating

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 9409, Derwent World Patents Index; Class A82, AN 94-072114, XP002024460 *
DATABASE WPI Section Ch Week 9413, Derwent World Patents Index; Class A23, AN 94-106899, XP002024461 *
PATENT ABSTRACTS OF JAPAN vol. 96, no. 7 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999018162A1 (en) * 1997-10-03 1999-04-15 E.I. Du Pont De Nemours And Company, Inc. Biocidal powder coating composition, its preparation and use
EP1353761A1 (en) * 2000-11-15 2003-10-22 SSW Holding Company, Inc. Coating with anti-microbial agent for refrigerator shelving
EP1353761A4 (en) * 2000-11-15 2004-10-20 Ssw Holding Co Inc Coating with anti-microbial agent for refrigerator shelving
US7357949B2 (en) 2001-12-21 2008-04-15 Agion Technologies Inc. Encapsulated inorganic antimicrobial additive for controlled release
US8063116B2 (en) 2006-08-25 2011-11-22 Sciessent Llc Antimicrobial powder coatings and method
US8518449B2 (en) 2006-08-25 2013-08-27 Sciessent Llc Polymer particle coating method
GB2442440A (en) * 2006-10-04 2008-04-09 Philip Reed Anti-microbial cover for door furniture
EP1925201A1 (en) * 2006-11-09 2008-05-28 Werner Zapletal Method for antimicrobial treatment of powder
US9861102B2 (en) 2016-05-26 2018-01-09 Markesbery Blue Pearl LLC Methods for disinfection
US10603396B2 (en) 2016-05-26 2020-03-31 Markesbery Blue Pearl LLC Methods and system for disinfection
US11425911B2 (en) 2017-05-25 2022-08-30 Markesbery Blue Pearl LLC Method for disinfection of items and spaces

Also Published As

Publication number Publication date
GB9825646D0 (en) 1999-01-13
GB2328691B (en) 2000-12-06
FI982617A (en) 1999-02-02
GB2328691A8 (en) 1999-03-25
PT904323E (en) 2002-01-30
DE69614004T2 (en) 2002-03-21
AU6010096A (en) 1998-01-05
EP0904323B1 (en) 2001-07-18
JP2000511554A (en) 2000-09-05
ATE203263T1 (en) 2001-08-15
ES2160248T3 (en) 2001-11-01
GB2328691A (en) 1999-03-03
BR9612636A (en) 1999-07-20
FI982617A0 (en) 1998-12-04
US6129782A (en) 2000-10-10
CA2258256A1 (en) 1997-12-11
AU734162B2 (en) 2001-06-07
CA2258256C (en) 2002-02-26
EP0904323A1 (en) 1999-03-31
US5980620A (en) 1999-11-09
DK0904323T3 (en) 2001-11-05
DE69614004D1 (en) 2001-08-23
GR3036913T3 (en) 2002-01-31

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