WO1997036170A1 - Appareil a electrophorese - Google Patents

Appareil a electrophorese Download PDF

Info

Publication number
WO1997036170A1
WO1997036170A1 PCT/US1997/004934 US9704934W WO9736170A1 WO 1997036170 A1 WO1997036170 A1 WO 1997036170A1 US 9704934 W US9704934 W US 9704934W WO 9736170 A1 WO9736170 A1 WO 9736170A1
Authority
WO
WIPO (PCT)
Prior art keywords
buffer tank
electrophoresis
gel
disposed
lid
Prior art date
Application number
PCT/US1997/004934
Other languages
English (en)
Inventor
Arthur H. Haeffner, Jr.
Michael W. Schuette
Original Assignee
Life Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies, Inc. filed Critical Life Technologies, Inc.
Priority to AU25489/97A priority Critical patent/AU2548997A/en
Publication of WO1997036170A1 publication Critical patent/WO1997036170A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots

Definitions

  • the first and second electrodes and first and second conductors may be made from a nickel foil having an adhesive on one side so that the electrodes and the conductors may be securely attached to the buffer tank and the lid.
  • the electrodes corrode and become unusable after several runs, either the electrodes or the entire apparatus may be replaced.
  • the substantially vertical and substantially horizontal electrophoresis apparatus could also be loaded and run in an automated system.
  • the registration key is used by the automated system to locate the wells in the gel relative to the apparatus.
  • An automated loader having micropipette tips automatically loads the samples to be tested into the wells.
  • a device can be used to puncture holes in the lid through which the samples may be loaded.
  • the lid is removed before loading the samples in the wells.
  • the automated system then applies the necessary electricity to run the electrophoresis process.
  • the automated system may also photograph the results ofthe testing for subsequent study and analysis.
  • first conductor 204 and second conductor 208 are shown on the first side of lip 112.
  • First electrode 404 is shown adjacent first conductor 204 on lip 1 12.
  • First electrode 404 extends downwardly into an electrophoretic area 420 created inside buffer tank 104.
  • second electrode 408 is shown adjacent second conductor 208 on lip 112.
  • Second electrode 408 extends downwardly into electrophoretic area 420. Electrodes 404 and 408 extend downwardly all the way to the bottom of buffer tank 104 so that when buffer solution is poured into buffer tank 104, a portion of electrodes 404 and 408 come into contact with the buffer solution.
  • the electrodes can be made from Nickel tape. Altematively, the electrodes could be made from stainless steel tape. As discussed above with respect to the conductors, other metal foil materials can be used to make the electrodes, as would be apparent to one of skilled in the relevant art. Similarly, as discussed above, metal materials can be deposited onto the buffer tank to form the electrodes ofthe present invention.
  • FIG. 5 is a simplified perspective view of buffer tank 104 showing electrophoretic area 420.
  • first electrode 404 is an L-shaped piece of material. A small portion of first conductor 204 is also visible on lip 112.
  • second electrode 408 is not visible from this perspective view, in the preferred embodiment, the shape of second electrode 408 matches that shown of first electrode 404.
  • Second electrode 1304 extends downwardly from the bottom of lid 1208 so that when lid 1208 is placed on top of buffer tank 1204, second electrode 1304 contacts the buffer solution in tray 1250 to complete the connection.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention porte sur un appareil à électrophorèse (100). Un premier mode de réalisation de la présente invention est utilisé pour séparer des protéines ou des acides nucléiques par électrophorèse. Ce mode de réalisation comprend un réservoir tampon (104) qui contient une zone d'électrophorèse. Une première électrode est montée sur une première partie de l'appareil et une seconde électrode est montée sur une seconde partie de l'appareil. Un premier conducteur et un second conducteur sont placés sur le réservoir tampon (104) et fournissent des voies conductrices partant d'une source d'énergie (120) pour aller, respectivement, à la première électrode et à la seconde électrode. Un gel est introduit dans le réservoir tampon (104) et deux coussinets de mousse à alvéoles ouvertes, saturés dans une solution tampon, sont placés dans le réservoir tampon (104). Un couvercle (136) vient fermer le dessus du réservoir tampon.
PCT/US1997/004934 1996-03-27 1997-03-27 Appareil a electrophorese WO1997036170A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU25489/97A AU2548997A (en) 1996-03-27 1997-03-27 Electrophoresis apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US62235896A 1996-03-27 1996-03-27
US08/622,358 1996-03-27

Publications (1)

Publication Number Publication Date
WO1997036170A1 true WO1997036170A1 (fr) 1997-10-02

Family

ID=24493887

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/004934 WO1997036170A1 (fr) 1996-03-27 1997-03-27 Appareil a electrophorese

Country Status (2)

Country Link
AU (1) AU2548997A (fr)
WO (1) WO1997036170A1 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000068677A1 (fr) * 1999-05-06 2000-11-16 University Of Southampton Gel d'electrophorese
WO2001016589A1 (fr) * 1999-09-01 2001-03-08 Mirador Dna Design Inc. Cassette d'electrophorese thermoformees a jeter
WO2003066514A2 (fr) * 2002-02-08 2003-08-14 University Of Louisville Research Foundation, Inc.Belknap Campus Dispositif a puce de detection electrochimique par electrophorese capillaire et circuits de support
WO2003074257A1 (fr) * 2002-03-07 2003-09-12 Mirador Dna Design Inc. Appareil de fabrication d'une plaque d'electrophorese jetable et procede de fabrication
US7988839B2 (en) 2005-09-20 2011-08-02 University Of Louisville Research Foundation, Inc. Capillary electrophoresis systems and methods
EP2856130A4 (fr) * 2012-05-31 2016-01-06 Ge Healthcare Bio Sciences Ab Plateau d'électrophorèse et procédé de réalisation d'une expérience d'électrophorèse
US20160231272A1 (en) * 2015-02-10 2016-08-11 Bio-Rad Laboratories, Inc. Dry protein transfer
WO2022040062A1 (fr) * 2020-08-19 2022-02-24 Helena Laboratories Corporation Tampon de buvard pour électrophorèse
WO2023069756A1 (fr) * 2021-10-21 2023-04-27 Zadeh Cameron O Appareil de gel électrophorétique à multiplexage à haut débit et procédés associés

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3407133A (en) * 1965-06-18 1968-10-22 Baxter Laboratories Inc Expendable electrophoresis apparatus
US3715295A (en) * 1971-09-02 1973-02-06 Tlc Corp Disposable electrophoresis unit
US4622124A (en) * 1985-01-11 1986-11-11 International Biotechnologies, Inc. Device for horizontal electroblotting
US5013420A (en) * 1990-04-19 1991-05-07 Life Technologies Inc. Gel electrophoresis/electro-blot apparatus

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3407133A (en) * 1965-06-18 1968-10-22 Baxter Laboratories Inc Expendable electrophoresis apparatus
US3715295A (en) * 1971-09-02 1973-02-06 Tlc Corp Disposable electrophoresis unit
US4622124A (en) * 1985-01-11 1986-11-11 International Biotechnologies, Inc. Device for horizontal electroblotting
US5013420A (en) * 1990-04-19 1991-05-07 Life Technologies Inc. Gel electrophoresis/electro-blot apparatus

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000068677A1 (fr) * 1999-05-06 2000-11-16 University Of Southampton Gel d'electrophorese
GB2353866A (en) * 1999-05-06 2001-03-07 Univ Southampton Gel electrophoresis
GB2353866B (en) * 1999-05-06 2003-12-03 Univ Southampton Gel electrophoresis
WO2001016589A1 (fr) * 1999-09-01 2001-03-08 Mirador Dna Design Inc. Cassette d'electrophorese thermoformees a jeter
WO2003066514A2 (fr) * 2002-02-08 2003-08-14 University Of Louisville Research Foundation, Inc.Belknap Campus Dispositif a puce de detection electrochimique par electrophorese capillaire et circuits de support
WO2003066514A3 (fr) * 2002-02-08 2004-07-08 Univ Louisville Res Found Dispositif a puce de detection electrochimique par electrophorese capillaire et circuits de support
US7344628B2 (en) 2002-02-08 2008-03-18 The University Of Louisville Research Foundation Capillary electrophoresis-electrochemical detection microchip device and supporting circuits
WO2003074257A1 (fr) * 2002-03-07 2003-09-12 Mirador Dna Design Inc. Appareil de fabrication d'une plaque d'electrophorese jetable et procede de fabrication
US7988839B2 (en) 2005-09-20 2011-08-02 University Of Louisville Research Foundation, Inc. Capillary electrophoresis systems and methods
EP2856130A4 (fr) * 2012-05-31 2016-01-06 Ge Healthcare Bio Sciences Ab Plateau d'électrophorèse et procédé de réalisation d'une expérience d'électrophorèse
US9360452B2 (en) 2012-05-31 2016-06-07 Ge Healthcare Bio-Sciences Ab Electrophoresis tray and a method of running an electrophoresis experiment
US20160231272A1 (en) * 2015-02-10 2016-08-11 Bio-Rad Laboratories, Inc. Dry protein transfer
CN107209144A (zh) * 2015-02-10 2017-09-26 生物辐射实验室股份有限公司 干法蛋白质转移
US9945809B2 (en) * 2015-02-10 2018-04-17 Bio-Rad Laboratories, Inc. Dry protein transfer
CN107209144B (zh) * 2015-02-10 2021-02-19 生物辐射实验室股份有限公司 干法蛋白质转移
WO2022040062A1 (fr) * 2020-08-19 2022-02-24 Helena Laboratories Corporation Tampon de buvard pour électrophorèse
WO2023069756A1 (fr) * 2021-10-21 2023-04-27 Zadeh Cameron O Appareil de gel électrophorétique à multiplexage à haut débit et procédés associés

Also Published As

Publication number Publication date
AU2548997A (en) 1997-10-17

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