WO1997036170A1 - Appareil a electrophorese - Google Patents
Appareil a electrophorese Download PDFInfo
- Publication number
- WO1997036170A1 WO1997036170A1 PCT/US1997/004934 US9704934W WO9736170A1 WO 1997036170 A1 WO1997036170 A1 WO 1997036170A1 US 9704934 W US9704934 W US 9704934W WO 9736170 A1 WO9736170 A1 WO 9736170A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- buffer tank
- electrophoresis
- gel
- disposed
- lid
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots
Definitions
- the first and second electrodes and first and second conductors may be made from a nickel foil having an adhesive on one side so that the electrodes and the conductors may be securely attached to the buffer tank and the lid.
- the electrodes corrode and become unusable after several runs, either the electrodes or the entire apparatus may be replaced.
- the substantially vertical and substantially horizontal electrophoresis apparatus could also be loaded and run in an automated system.
- the registration key is used by the automated system to locate the wells in the gel relative to the apparatus.
- An automated loader having micropipette tips automatically loads the samples to be tested into the wells.
- a device can be used to puncture holes in the lid through which the samples may be loaded.
- the lid is removed before loading the samples in the wells.
- the automated system then applies the necessary electricity to run the electrophoresis process.
- the automated system may also photograph the results ofthe testing for subsequent study and analysis.
- first conductor 204 and second conductor 208 are shown on the first side of lip 112.
- First electrode 404 is shown adjacent first conductor 204 on lip 1 12.
- First electrode 404 extends downwardly into an electrophoretic area 420 created inside buffer tank 104.
- second electrode 408 is shown adjacent second conductor 208 on lip 112.
- Second electrode 408 extends downwardly into electrophoretic area 420. Electrodes 404 and 408 extend downwardly all the way to the bottom of buffer tank 104 so that when buffer solution is poured into buffer tank 104, a portion of electrodes 404 and 408 come into contact with the buffer solution.
- the electrodes can be made from Nickel tape. Altematively, the electrodes could be made from stainless steel tape. As discussed above with respect to the conductors, other metal foil materials can be used to make the electrodes, as would be apparent to one of skilled in the relevant art. Similarly, as discussed above, metal materials can be deposited onto the buffer tank to form the electrodes ofthe present invention.
- FIG. 5 is a simplified perspective view of buffer tank 104 showing electrophoretic area 420.
- first electrode 404 is an L-shaped piece of material. A small portion of first conductor 204 is also visible on lip 112.
- second electrode 408 is not visible from this perspective view, in the preferred embodiment, the shape of second electrode 408 matches that shown of first electrode 404.
- Second electrode 1304 extends downwardly from the bottom of lid 1208 so that when lid 1208 is placed on top of buffer tank 1204, second electrode 1304 contacts the buffer solution in tray 1250 to complete the connection.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU25489/97A AU2548997A (en) | 1996-03-27 | 1997-03-27 | Electrophoresis apparatus |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US62235896A | 1996-03-27 | 1996-03-27 | |
US08/622,358 | 1996-03-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1997036170A1 true WO1997036170A1 (fr) | 1997-10-02 |
Family
ID=24493887
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1997/004934 WO1997036170A1 (fr) | 1996-03-27 | 1997-03-27 | Appareil a electrophorese |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU2548997A (fr) |
WO (1) | WO1997036170A1 (fr) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000068677A1 (fr) * | 1999-05-06 | 2000-11-16 | University Of Southampton | Gel d'electrophorese |
WO2001016589A1 (fr) * | 1999-09-01 | 2001-03-08 | Mirador Dna Design Inc. | Cassette d'electrophorese thermoformees a jeter |
WO2003066514A2 (fr) * | 2002-02-08 | 2003-08-14 | University Of Louisville Research Foundation, Inc.Belknap Campus | Dispositif a puce de detection electrochimique par electrophorese capillaire et circuits de support |
WO2003074257A1 (fr) * | 2002-03-07 | 2003-09-12 | Mirador Dna Design Inc. | Appareil de fabrication d'une plaque d'electrophorese jetable et procede de fabrication |
US7988839B2 (en) | 2005-09-20 | 2011-08-02 | University Of Louisville Research Foundation, Inc. | Capillary electrophoresis systems and methods |
EP2856130A4 (fr) * | 2012-05-31 | 2016-01-06 | Ge Healthcare Bio Sciences Ab | Plateau d'électrophorèse et procédé de réalisation d'une expérience d'électrophorèse |
US20160231272A1 (en) * | 2015-02-10 | 2016-08-11 | Bio-Rad Laboratories, Inc. | Dry protein transfer |
WO2022040062A1 (fr) * | 2020-08-19 | 2022-02-24 | Helena Laboratories Corporation | Tampon de buvard pour électrophorèse |
WO2023069756A1 (fr) * | 2021-10-21 | 2023-04-27 | Zadeh Cameron O | Appareil de gel électrophorétique à multiplexage à haut débit et procédés associés |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3407133A (en) * | 1965-06-18 | 1968-10-22 | Baxter Laboratories Inc | Expendable electrophoresis apparatus |
US3715295A (en) * | 1971-09-02 | 1973-02-06 | Tlc Corp | Disposable electrophoresis unit |
US4622124A (en) * | 1985-01-11 | 1986-11-11 | International Biotechnologies, Inc. | Device for horizontal electroblotting |
US5013420A (en) * | 1990-04-19 | 1991-05-07 | Life Technologies Inc. | Gel electrophoresis/electro-blot apparatus |
-
1997
- 1997-03-27 AU AU25489/97A patent/AU2548997A/en not_active Abandoned
- 1997-03-27 WO PCT/US1997/004934 patent/WO1997036170A1/fr active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3407133A (en) * | 1965-06-18 | 1968-10-22 | Baxter Laboratories Inc | Expendable electrophoresis apparatus |
US3715295A (en) * | 1971-09-02 | 1973-02-06 | Tlc Corp | Disposable electrophoresis unit |
US4622124A (en) * | 1985-01-11 | 1986-11-11 | International Biotechnologies, Inc. | Device for horizontal electroblotting |
US5013420A (en) * | 1990-04-19 | 1991-05-07 | Life Technologies Inc. | Gel electrophoresis/electro-blot apparatus |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000068677A1 (fr) * | 1999-05-06 | 2000-11-16 | University Of Southampton | Gel d'electrophorese |
GB2353866A (en) * | 1999-05-06 | 2001-03-07 | Univ Southampton | Gel electrophoresis |
GB2353866B (en) * | 1999-05-06 | 2003-12-03 | Univ Southampton | Gel electrophoresis |
WO2001016589A1 (fr) * | 1999-09-01 | 2001-03-08 | Mirador Dna Design Inc. | Cassette d'electrophorese thermoformees a jeter |
WO2003066514A2 (fr) * | 2002-02-08 | 2003-08-14 | University Of Louisville Research Foundation, Inc.Belknap Campus | Dispositif a puce de detection electrochimique par electrophorese capillaire et circuits de support |
WO2003066514A3 (fr) * | 2002-02-08 | 2004-07-08 | Univ Louisville Res Found | Dispositif a puce de detection electrochimique par electrophorese capillaire et circuits de support |
US7344628B2 (en) | 2002-02-08 | 2008-03-18 | The University Of Louisville Research Foundation | Capillary electrophoresis-electrochemical detection microchip device and supporting circuits |
WO2003074257A1 (fr) * | 2002-03-07 | 2003-09-12 | Mirador Dna Design Inc. | Appareil de fabrication d'une plaque d'electrophorese jetable et procede de fabrication |
US7988839B2 (en) | 2005-09-20 | 2011-08-02 | University Of Louisville Research Foundation, Inc. | Capillary electrophoresis systems and methods |
EP2856130A4 (fr) * | 2012-05-31 | 2016-01-06 | Ge Healthcare Bio Sciences Ab | Plateau d'électrophorèse et procédé de réalisation d'une expérience d'électrophorèse |
US9360452B2 (en) | 2012-05-31 | 2016-06-07 | Ge Healthcare Bio-Sciences Ab | Electrophoresis tray and a method of running an electrophoresis experiment |
US20160231272A1 (en) * | 2015-02-10 | 2016-08-11 | Bio-Rad Laboratories, Inc. | Dry protein transfer |
CN107209144A (zh) * | 2015-02-10 | 2017-09-26 | 生物辐射实验室股份有限公司 | 干法蛋白质转移 |
US9945809B2 (en) * | 2015-02-10 | 2018-04-17 | Bio-Rad Laboratories, Inc. | Dry protein transfer |
CN107209144B (zh) * | 2015-02-10 | 2021-02-19 | 生物辐射实验室股份有限公司 | 干法蛋白质转移 |
WO2022040062A1 (fr) * | 2020-08-19 | 2022-02-24 | Helena Laboratories Corporation | Tampon de buvard pour électrophorèse |
WO2023069756A1 (fr) * | 2021-10-21 | 2023-04-27 | Zadeh Cameron O | Appareil de gel électrophorétique à multiplexage à haut débit et procédés associés |
Also Published As
Publication number | Publication date |
---|---|
AU2548997A (en) | 1997-10-17 |
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