WO1997024621A3 - Reagents for measuring lead levels via the quantification of porphobilinogen - Google Patents

Reagents for measuring lead levels via the quantification of porphobilinogen Download PDF

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Publication number
WO1997024621A3
WO1997024621A3 PCT/US1996/019544 US9619544W WO9724621A3 WO 1997024621 A3 WO1997024621 A3 WO 1997024621A3 US 9619544 W US9619544 W US 9619544W WO 9724621 A3 WO9724621 A3 WO 9724621A3
Authority
WO
WIPO (PCT)
Prior art keywords
porphobilinogen
quantification
reagents
fluorescein
levels via
Prior art date
Application number
PCT/US1996/019544
Other languages
French (fr)
Other versions
WO1997024621A2 (en
Inventor
Maciej Adamczyk
Jeffrey R Fishpaugh
Kevin Heuser
John M Ramp
Martin Wong
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Priority to EP96943648A priority Critical patent/EP0876616A2/en
Priority to JP9524338A priority patent/JP2000506603A/en
Publication of WO1997024621A2 publication Critical patent/WO1997024621A2/en
Publication of WO1997024621A3 publication Critical patent/WO1997024621A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D207/00—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D207/02—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D207/30—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
    • C07D207/32—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C07D207/33—Heterocyclic compounds containing five-membered rings not condensed with other rings, with one nitrogen atom as the only ring hetero atom with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms with substituted hydrocarbon radicals, directly attached to ring carbon atoms
    • C07D207/337—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

Immunoassay methods and reagents for measuring the levels of lead in a test sample via the quantification of porphobilinogen use antibodies prepared with compounds of Formulas (II) and (III), wherein X and Y are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to an immunogenic carrier material P wherein P is preferably bovine serum albumin. Also described are the synthesis of fluorescein tracers of the structure of Formulas (V) and (VII), wherein A and C are linking groups consisting of 0 to 2 heteroatoms and 0 to 8 carbon atoms linked to a detectable moiety Q, and wherein Q is a detectable moiety, preferably fluorescein or a fluorescein derivative.
PCT/US1996/019544 1995-12-29 1996-12-16 Reagents for measuring lead levels via the quantification of porphobilinogen WO1997024621A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP96943648A EP0876616A2 (en) 1995-12-29 1996-12-16 Reagents for measuring lead levels via the quantification of porphobilinogen
JP9524338A JP2000506603A (en) 1995-12-29 1996-12-16 A reagent for measuring lead levels by porphobilinogen determination

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58115195A 1995-12-29 1995-12-29
US08/581,151 1995-12-29

Publications (2)

Publication Number Publication Date
WO1997024621A2 WO1997024621A2 (en) 1997-07-10
WO1997024621A3 true WO1997024621A3 (en) 1997-08-14

Family

ID=24324093

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/019544 WO1997024621A2 (en) 1995-12-29 1996-12-16 Reagents for measuring lead levels via the quantification of porphobilinogen

Country Status (4)

Country Link
EP (1) EP0876616A2 (en)
JP (1) JP2000506603A (en)
CA (1) CA2239969A1 (en)
WO (1) WO1997024621A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1357110A1 (en) * 2002-04-24 2003-10-29 Reinhard Neier Synthesis for the preparation of porphobilinogen, the compound as such as stabilized salt and intermediates thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993001310A1 (en) * 1991-07-11 1993-01-21 University Of Maryland At Baltimore Lead assay
WO1995004159A1 (en) * 1993-08-03 1995-02-09 Fox Chase Cancer Center Blood lead diagnostic assay
WO1995017679A1 (en) * 1993-12-21 1995-06-29 Abbott Laboratories Automated lead assay

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993001310A1 (en) * 1991-07-11 1993-01-21 University Of Maryland At Baltimore Lead assay
US5354652A (en) * 1991-07-11 1994-10-11 The University Of Maryland At Baltimore Lead assay
WO1995004159A1 (en) * 1993-08-03 1995-02-09 Fox Chase Cancer Center Blood lead diagnostic assay
WO1995017679A1 (en) * 1993-12-21 1995-06-29 Abbott Laboratories Automated lead assay

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
J. R. FISHPAUGH ET AL.: "Synthesis of porphobilinogen immunocomponents for the development of a lead assay.", ABSTRACTS OF PAPERS AMERICAN CHEMICAL SOCIETY, vol. 212, no. 1-2, 25 August 1996 (1996-08-25) - 29 August 1996 (1996-08-29), pages orgn 360, XP002032435 *
M. ADAMCZYK ET AL.: "Preparation of 2-carboxy-3,4-substituted pyrrole haptens and synthesis of porphobilinogen.", TETRAHEDRON LETTERS, vol. 37, no. 14, 1 April 1996 (1996-04-01), pages 2325 - 2326, XP002032434 *

Also Published As

Publication number Publication date
JP2000506603A (en) 2000-05-30
EP0876616A2 (en) 1998-11-11
WO1997024621A2 (en) 1997-07-10
CA2239969A1 (en) 1997-07-10

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