FR2348494A1 - Glycine-conjugated bile acids determination - using radioactively labelled tyramine derivs. of glycine-conjugated bile acids (BE 17.10.77) - Google Patents

Glycine-conjugated bile acids determination - using radioactively labelled tyramine derivs. of glycine-conjugated bile acids (BE 17.10.77)

Info

Publication number
FR2348494A1
FR2348494A1 FR7711324A FR7711324A FR2348494A1 FR 2348494 A1 FR2348494 A1 FR 2348494A1 FR 7711324 A FR7711324 A FR 7711324A FR 7711324 A FR7711324 A FR 7711324A FR 2348494 A1 FR2348494 A1 FR 2348494A1
Authority
FR
France
Prior art keywords
glycine
conjugated bile
tyramine
bile acid
bile acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7711324A
Other languages
French (fr)
Other versions
FR2348494B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of FR2348494A1 publication Critical patent/FR2348494A1/en
Application granted granted Critical
Publication of FR2348494B1 publication Critical patent/FR2348494B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Measuring the content of a specific, glycine-conjugated bile acid selected from cholylglycine (I), chenodeoxycholylglycine (II), deoxycholylglycine (III) and sulpholithocholylglycine (IV) in a sample comprises (a) providing an antiserum which is specific for one of the glycine-conjugated bile acids. Process further comprises (b) mixing the sample with the antiserum and with a tyramine deriv. of the same glycine-conjugated bile acid, the tyramine deriv. being labelled with a labelling agent; (c) incubating the mixt. so that the antibodies of the antiserum are bound to the glycine-conjugated bile acid of the sample and the labelled glycine-conjugated bile acid tyramine deriv. Process further comprises (d) the bound antibodies and the unbound material into separate fractions; and (e) measuring the radioactivity of one of the sepd. fractions the amt. of the specific bile acid conjugate being determined by comparison with standard samples. Sulpholithocholylglycyltryamine (IVa), chenodeoxycholylglycyltyramine (IIa), cholylglycyltyramine (Ia) and deoxycholylglycyltyramine (IIIa) are claimed. Process is used in the determn. of the ratio of prim. to sec. bile a acid conjugates, which in turn is useful in the diagnosis of hepatic damage due to infection or chemicals. Method can be used directly on serum sample, and can be carried out directly on analytical instruments without special solns. or ampoules.
FR7711324A 1976-04-16 1977-04-14 Glycine-conjugated bile acids determination - using radioactively labelled tyramine derivs. of glycine-conjugated bile acids (BE 17.10.77) Granted FR2348494A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US67758676A 1976-04-16 1976-04-16

Publications (2)

Publication Number Publication Date
FR2348494A1 true FR2348494A1 (en) 1977-11-10
FR2348494B1 FR2348494B1 (en) 1983-06-24

Family

ID=24719331

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7711324A Granted FR2348494A1 (en) 1976-04-16 1977-04-14 Glycine-conjugated bile acids determination - using radioactively labelled tyramine derivs. of glycine-conjugated bile acids (BE 17.10.77)

Country Status (4)

Country Link
JP (1) JPS5851000B2 (en)
BE (1) BE853669A (en)
DE (1) DE2716801C3 (en)
FR (1) FR2348494A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2916783A1 (en) * 1978-04-26 1979-10-31 Abbott Lab BALIC ACID TEST

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0256292U (en) * 1988-10-14 1990-04-24
JP2638521B2 (en) * 1994-11-24 1997-08-06 有限会社ビー・エス・アール Determination of liver disease by measurement of serum bile acids by ELISA
CN108982860A (en) * 2018-07-20 2018-12-11 北京百奥泰康生物技术有限公司 A kind of reagent using latex immunoturbidimetry technology measurement human serum content of glycocholic acid
CN108982859A (en) * 2018-07-20 2018-12-11 北京百奥泰康生物技术有限公司 A kind of reagent measuring human serum content of glycocholic acid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2164705A1 (en) * 1971-12-21 1973-08-03 Abbott Lab

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2164705A1 (en) * 1971-12-21 1973-08-03 Abbott Lab

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CA1974 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2916783A1 (en) * 1978-04-26 1979-10-31 Abbott Lab BALIC ACID TEST
FR2424536A1 (en) * 1978-04-26 1979-11-23 Abbott Lab METHOD FOR DETERMINING BILIARY ACIDS IN BIOLOGICAL FLUIDS

Also Published As

Publication number Publication date
DE2716801C3 (en) 1981-07-16
JPS52128215A (en) 1977-10-27
BE853669A (en) 1977-10-17
DE2716801B2 (en) 1980-07-03
FR2348494B1 (en) 1983-06-24
JPS5851000B2 (en) 1983-11-14
DE2716801A1 (en) 1977-10-20

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